References of "1982"
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See detailEtude isoenzymatique de la creatine kinase chez les traumatises craniens en coma.
Hans, Pol ULg; Born, J. D.; Chapelle, Jean-Paul ULg et al

in Neuro-Chirurgie (1982), 28(1), 9-12

We measured the c.s.f. and serum creatine kinase activities in the first hours following the trauma, for 24 severe head injury patients. We found a good correlation between total CK and CKBB isoenzymes ... [more ▼]

We measured the c.s.f. and serum creatine kinase activities in the first hours following the trauma, for 24 severe head injury patients. We found a good correlation between total CK and CKBB isoenzymes levels in the c.s.f. only. The c.s.f. values are a quantitative index of brain dysfunction assessed by the Liege coma scale, associating the Glasgow coma scale and the study of the brain stem reflexes. There is no relationship between CK activity and intracranial pressure. [less ▲]

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See detailThe sodium-potassium cotransport in red blood celles of hypertensive patients: advantages and limits
Krzesinski, Jean-Marie ULg; Rorive, Georges ULg

in Belgian G.D.R. Medical Week, Ministry of Public Health and Family (1982)

Established essential HTA is considered to result from the influence of both heredity and environment. Among the environmental factors, dietetary sodium has been proposed to play an important role ... [more ▼]

Established essential HTA is considered to result from the influence of both heredity and environment. Among the environmental factors, dietetary sodium has been proposed to play an important role inducing an increase in peripheral vascular resistance. The primary abnormality in essential HTA could consist in a genetically transmitted membrane defect impairing the cellular mechanisms which normally compensate a sodium load. The analysis of this defect implies more certainly elucidation of the effects of sodium ions at the blood vessel musculature level. But smooth muscle cells are unfortunately rather inaccessible. Yet it would be of great interest to dispose of a simple laboratory test to detect primary HTA. Therefore, on account of their availability and ease of manipulation, blood cells have been studied and already twenty years ago, LOSSE (12) recorded an increase of the intracellular sodium concentration in erythrocytes of hypertensive patients. Since then, different groups described different abnormalities' in the movement of ions across the blood cells membranes. Indeed modifications of the passive permeability (11, 12, 13), the sodium potassium pump (1, 5, 14), the sodium lithium countertransport (2, 7, 18) and the sodium potassium cotransport (3, 8, 9,10) have been successively demonstrated. GARAY et al. (9,10,11) observed a decrease in this last transport system for essential hypertensives and a high proportion of their young offspring. Conversely for normotensive patients, without a family history of HTA or in secondary hypertensives, a normal cotransport was found. The finding of an abnormal cotransport in a young normotensive may thus elicit preventive measures such as appropriate sodium diet, prevention of excessive weight gain, clinical follow up during oral contraception or pregnancy. The first observations showed a very clearcut difference between the two populations. However, many authors using the same test procedure, found a large overlap suggesting the measurement of this cotransport will not prove useful to discriminate primary HTA. However, the methodology was not always the same and difference could also arise from the definition of normal control groups which needed to be carefully selected. Thus in this study, we would evaluate the modification of the Na+ K+ cotransport in primary and secondary HTA, using strictly the experimental methodology originally described by GARAY et al. (9, 10). Afterwards, it would be interesting to study the effect of obesity, diabetes and the menstrual cycles on this transport system. [less ▲]

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See detailPurification and properties of the exocellular β-lactamase of Actinomadura strain R39
Duez, Colette ULg; Frère, Jean-Marie ULg; Ghuysen, Jean-Marie ULg et al

in Biochimica et Biophysica Acta - General Subjects (1982), 700

The exocellular beta-lactamase (penicillin amido-beta-lactamhydrolase, EC 3.5.2.6) of Actinomadura R39 consists of one single polypeptide chain of molecular weight about 15 200. It exhibits a highly ... [more ▼]

The exocellular beta-lactamase (penicillin amido-beta-lactamhydrolase, EC 3.5.2.6) of Actinomadura R39 consists of one single polypeptide chain of molecular weight about 15 200. It exhibits a highly asymmetrical shape, has a low isoelectric point (at pH 5.0) and contains about 9.3% (w/w) of a polydeoxyribonucleotide with which it forms a rather stable complex. Removal of a substantial amount of this deoxyribonucleotide by treatment with DNAase I has no effect on the enzyme activity. The beta-lactamase has a wide spectrum of activity. Penicillins and delta 3-cephalosporins can be either good or poor substrates. Oxacillin, which is a poor substrate of most beta-lactamases from Gram-positive bacteria, is a good substrate of the beta-lactamase of Actinomadura R39. Its best substrate, however, is nitrocefin (kcat/Km: 2300 000 M-1.s-1; catalytic centre activity: 210 s-1). The kcat/Km values observed with some penicillins and delta 3-cephalosporins are similar to the values of the bimolecular rate constants that govern the formation of the acyl-enzyme intermediates between these antibiotics and the serine D-alanyl-D-alanine peptidase that is also secreted by the same strain Actinomadura R39. Such a relationship, however, is not observed with all the beta-lactam compounds tested. [less ▲]

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See detailFacteurs climatiques et reproduction du Pinson des arbres (Fringilla coelebs L.) sur le plateau des Hautes-Fagnes
Metzmacher, Maxime ULg

in Cahiers d’Ethologie Appliquée (1982), 2

The influence of climatic and behavioural factors on the progress and success of the breeding cycle of chaffinches (Fringilla coelebs) has been investigated on the Hautes-Fagnes plateau (Belgium). From ... [more ▼]

The influence of climatic and behavioural factors on the progress and success of the breeding cycle of chaffinches (Fringilla coelebs) has been investigated on the Hautes-Fagnes plateau (Belgium). From april to july 1980, three line transects (two of 1,6 and one of 1,2 km) in Norway spruce plantations were regularly prospected. The estimation of the number of territorial males was improved by a sonographic analysis of their songs, The breeding season has been marked twice by unfavourable weather conditions. In april, a cold-wave produced flocks reformation, but after then the cycle could evolve normally. In june-july, heavy rains seem to have affected many broods. The 1968 (watched by Mairy) and 1980 breeding cycles have been compared ; both emphazise the Chaffinch's breeding cycle sensitivity to climatic hazards. [less ▲]

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See detailHydrolyse Enzymatique de la Cellulose régénérée.
Paquot, Michel ULg; Thonart, Philippe ULg

in Holzforschung : International Journal of the Biology, Chemistry, Physics, & Technology of Wood (1982), 36

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See detailPatients with myocardial infarction and normal coronary arteriogram.
Legrand, Victor ULg; Deliege, M.; Henrard, L. et al

in Chest (1982), 82(6), 678-85

Eighteen patients who survived an acute myocardial infarction were found to have a normal coronary arteriogram. Seven patients were younger than 35 years and six were female. The myocardial infarction was ... [more ▼]

Eighteen patients who survived an acute myocardial infarction were found to have a normal coronary arteriogram. Seven patients were younger than 35 years and six were female. The myocardial infarction was nontransmural in 11 cases. The mean follow-up was 21.6 months. Eleven patients developed residual chest pain at rest early after myocardial infarction. One, treated by beta-blockers, suffered a recurrent myocardial infarction. Eight became asymptomatic, and two improved under antispastic therapy. Another patient developed a severe form of variant angina three months after myocardial infarction; she died following plexectomy. Finally, two patients experienced rare episodes of angina at rest. The stress ECG was negative in all cases. Provocative test for spasm was positive in three out of nine patients. Diffuse narrowing associated with chest pain was demostrated in two patients at angiography. Thus, myocardial infarction and subsequent normal coronary angiogram are mainly found in young female patients, and infarction is often nontransmural. Clinical evidence of vasospastic phenomena and increased vasomotor tone are found in most patients. Whenever residual chest pain is controlled by antispastic therapy, the follow-up course seems benign. [less ▲]

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See detailMéthodes d'analyse des localisations commerciales : les apports de l'enquête de terrain
Merenne-Schoumaker, Bernadette ULg

in Annales de Géographie (1982), (506), 417-424

An analysis of methods of commercial locations. The results of a ground survey. This article presents a survey and an outlook on commercial location in three differents sectors whereby the results are ... [more ▼]

An analysis of methods of commercial locations. The results of a ground survey. This article presents a survey and an outlook on commercial location in three differents sectors whereby the results are particularly interesting: spatial organization of equipments, dynamism of evolutions and motivations of differents locations. In every case, we have tried to demonstrate the methods to use and the primary possible methods that can be investigated. All methodolo¬gical problems are not yet resolved, but we can observe how the development of marketing geography of retailing depends on collecting technics and the modality of methods. [less ▲]

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See detailImmunolocalization of laminin, fibronectin, and collagens I to V in the normal and mastotic breast
Gordenne, W.; Foidart, Jean-Michel ULg; Lapière, C. M.

in Journal de Gynécologie, Obstétrique et Biologie de la Reproduction (1982), 11(5), 549-54

Our study tries by immunofluorescence to specify the nature of the connective tissue of the breasts, and especially of the scaffolding of the fatty tissue and the matrix of the lobules and of the ... [more ▼]

Our study tries by immunofluorescence to specify the nature of the connective tissue of the breasts, and especially of the scaffolding of the fatty tissue and the matrix of the lobules and of the lactiferous ducts. In consists of a study of 35 samples which were taken from 13 women whose ages ranged from 40 to 75 and whose breasts were considered on clinical examination and on radiography as normal or abnormal. We have analyzed the distribution of anti-collagen types I, III, IV and V and anti-fibronectin and anti-laminin antibodies in certain sites in the breast which were chosen because of the diversity of forms they could take from one woman to another. In each case of the distribution of protein markers seemed to be regular without, as can be found in certain cases of scar or tumour formation, a change in the distribution of the matrix proteins. Therefore, as already suggested by certain histologists, benign abnormal breast conditions (fibrosis) should be considered as simple variations from the normal. This conclusion agrees with classical histological findings. [less ▲]

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See detailBasement membrane components and galactosylhydroxylysyl glucosyltransferase in suction blisters of human skin.
Oikarinen, A.; Savolainen, E. R.; Tryggvason, K. et al

in British Journal of Dermatology (1982), 106(3), 257-66

Basement membrane components and collagen biosynthesis were studied in suction blisters in human skin. The basement membrane components were characterized by immunofluorescence using specific antibodies ... [more ▼]

Basement membrane components and collagen biosynthesis were studied in suction blisters in human skin. The basement membrane components were characterized by immunofluorescence using specific antibodies to type IV collagen, laminin and fibronectin, and collagen biosynthesis was studied by assaying galactosylhydroxylysyl glucosylatransferase. In suction blisters, the separation of epidermis and dermis occurred above the lamina lucida, indicating that the basement membrane, composed of lamina lucida and lamina densa, forms a mechanically strong entity. During the regeneration phase of blisters, type Iv collagen and laminin were not observed in the old epidermal blister roof. This indicates that keratinocytes when separated from the underlying basement membrane or connective tissue do not synthesize laminin or type IV collagen. Galactosylhydroxylysyl glucosyltransferase activity could be demonstrated in blister fluid and was about the same as in serum when expressed on the basis of protein in fresh blisters. It increased by 2-3 fold during the repair of blisters, indicating that there was local production of this enzyme. Further studies revealed that pure epidermis contained galactosylyhdroxylysyl glucosyltransferase and hydroxyprolineand this suggests that epidermis may synthesize some collagen type which, according to these studies, is not type IV (basement memebrane) collagen. [less ▲]

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See detailActinic granulomas and relapsing polychondritis.
Pierard, Gérald ULg; Henrijean, A.; Foidart, Jean-Michel ULg et al

in Acta Dermato Venereologica (1982), 62(6), 531-3

A patient developed concomitantly chondritis of the two auricles, diffuse cutaneous vasculitis and actinic granulomas. Alterations in skin and cartilage were prominent in the elastic tissue. Anticollagen ... [more ▼]

A patient developed concomitantly chondritis of the two auricles, diffuse cutaneous vasculitis and actinic granulomas. Alterations in skin and cartilage were prominent in the elastic tissue. Anticollagen type II antibodies were absent from the serum and there was no deposit of immunoreactants in cartilages. In this form of relapsing polychondritis, the pathomechanism resembles that of diffuse actinic arteritis as proposed by O'Brien. It is concluded that relapsing polychondritis may represent a heterogeneous syndrome with regard to its pathogenesis. [less ▲]

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See detailStimulation of retinoic acid of synthesis and turnover of basement membrane in mouse embryonal carcinoma-derived endoderm cells.
Salomon, D. S.; Liotta, L. A.; Rennard, S. I. et al

in Collagen and Related Research (1982), 2(2), 93-110

The effect of retinoic acid on the synthesis and degradation of basement membrane components by endoderm cells derived from mouse embryonal carcinoma (EC) cells was studied in a serum-free, defined medium ... [more ▼]

The effect of retinoic acid on the synthesis and degradation of basement membrane components by endoderm cells derived from mouse embryonal carcinoma (EC) cells was studied in a serum-free, defined medium. By immunofluorescence these cells accumulate type IV collagen, laminin, and fibronectin after growth in media containing epidermal growth factor (EGF), fibroblast growth factor (FGF), insulin, transferrin, and Pedersen fetuin. Collagen accounted for 2 to 4% of the newly synthesized proteins, of which 90% were found in the culture media. This collagen was identified as Pro-type IV be gel electrophoresis and enzymatic susceptibility. The EC cells preferentially attached to type IV collagen in vitro and such attachment was mediated by laminin. Treatment of EC cells with retinoic acid caused an increased accumulation of collagen (10 to 15% of secreted proteins) and also stimulated the elaboration of latent protease which degraded laminin and type IV collagen. The laminin-degrading activity was plasminogen dependent. The type IV collagen-degrading activity was a metal protease which could be activated by trypsin or plasmin. It is likely that at least part of the laminin degrading activity is plasmin (mediated through plasminogen activator), since highly purified plasmin is shown to degrade native laminin. [less ▲]

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See detailMagnetic solid-phase enzyme immunoassay for the detection of anti-glomerular basement membrane antibodies.
Druet, E.; Mahieu, P.; Foidart, Jean-Michel ULg et al

in Journal of Immunological Methods (1982), 48(2), 149-57

A new enzyme immunoassay has been developed for the demonstration of antiglomerular basement membrane antibodies. Magnetically responsive polyacrylamide-agarose beads (Magnogel) activated with ... [more ▼]

A new enzyme immunoassay has been developed for the demonstration of antiglomerular basement membrane antibodies. Magnetically responsive polyacrylamide-agarose beads (Magnogel) activated with glutaraldehyde were used to bind sonicated insoluble rat glomerular basement membranes. Both the collagenous and the non-collagenous moieties were demonstrated to be fixed on the beads. Sera from brown Norway rats with anti-glomerular basement membrane antibodies induced by HgCl2 injections were incubated with the beads. After washing, the fixed rat IgG were revealed using alkaline phosphatase labelled Fab fragments from anti-rat IgG sheep, IgGs. Comparison with a radioimmunoassay showed that results were reliable. This enzyme immunoassay has several advantages which may render this assay of considerable clinical usefulness. [less ▲]

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See detailDemonstration of laminin, a basement membrane glycoprotein, in routinely processed formalin-fixed human tissues.
Ekblom, P.; Miettinen, M.; Rapola, J. et al

in Histochemistry (1982), 75(3), 301-7

Laminin was demonstrated by immunoperoxidase and immunofluorescence staining in sections of normal human tissues fixed in formalin and routinely processed in paraffin. Exposure of the sections to a ... [more ▼]

Laminin was demonstrated by immunoperoxidase and immunofluorescence staining in sections of normal human tissues fixed in formalin and routinely processed in paraffin. Exposure of the sections to a solution of pepsin (Burns et al. (1980) Histochemistry 67:73-78) revealed the antigenicity of this basement membrane glycoprotein. Sections from paraffin blocks stored for years at room temperature could be stained with this procedure. Normal human tissues, developing fetal tissues and tumors could be stained with this method. The staining patterns were similar to those seen in unfixed frozen sections. It thus appears that basement membrane components can be detected by immunohistological means from routinely processed histological samples, once the sections are pretreated with proteases. Staining for laminin could be used in embryonic studies and in histopathology to study the relation of cells to basement membranes and for the visualization of normal and abnormal vascularization. [less ▲]

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See detailEvidence for a particular binding capacity of rat peritoneal macrophages to rat glomerular mesangial cells in vitro.
Dubois, Ch; Goffinet, G.; Foidart, J. B. et al

in European Journal of Clinical Investigation (1982), 12(3), 239-46

The adhesion of normal rat peritoneal macrophages to normal rat glomerular epithelial or mesangial cells has been studied in vitro after a 60 min incubation at 37 degree C. After washing, the cell ... [more ▼]

The adhesion of normal rat peritoneal macrophages to normal rat glomerular epithelial or mesangial cells has been studied in vitro after a 60 min incubation at 37 degree C. After washing, the cell preparations were examined by phase contrast or scanning electron microscopy. Quantitative studies were also performed using macrophages labelled with 99mTc tin colloids. Peritoneal macrophages predominantly adhered to the cultured mesangial cells. The percent-age of labelled macrophages adhering to these cells was about 10 times higher than that of labelled macrophages adhering to the cultured epithelial cells. This percentage increased proportionally to the number of labelled macrophages added, and was strongly reduced by the prior incubation of macrophagic cells with aggregated IgG, with anti-fibronectin IgG, or with F(ab')2 fragments of anti-fibronectin IgG. Furthermore, the macrophage-mesangial cell interaction was significantly reduced by the prior incubation of mesangial cells with anti-fibronectin IgG or with F(ab')2 fragments of anti-fibronectin IgG. The data demonstrate that normal rat peritoneal macrophages preferentially adhere in vitro to normal rat glomerular mesangial cells, and that this binding may be modulated, at least, by: (a) the Fc receptor binding activity of macrophages; (b) the fibronectin molecules available at the surface of macrophages and of mesangial cells. [less ▲]

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See detailImmunofluorescence localization of fibronectin in chondrosarcoma cartilage matrix.
Kimata, K.; Foidart, Jean-Michel ULg; Pennypacker, J. P. et al

in Cancer Research (1982), 42(6), 2384-91

In this study, we have compared the extracellular matrix components and the in vitro adhesion characteristics of normal rat epiphysial chondrocytes with those from the Swarm rat chondrosarcoma, which has ... [more ▼]

In this study, we have compared the extracellular matrix components and the in vitro adhesion characteristics of normal rat epiphysial chondrocytes with those from the Swarm rat chondrosarcoma, which has many of the biochemical characteristics of normal cartilage. With the use of immunofluorescence techniques, tissue slices and chondrocytes in culture were tested for the presence of collagen types I and II, cartilage-characteristic proteoglycan, and fibronectin. Both normal and tumor matrix contained type II collagen and cartilage proteoglycan, but only the tumor matrix contained fibronectin. In culture, tumor-derived chondrocytes continued to accumulate fibronectin in their matrix, even after deposition of type II collagen and proteoglycans, while normal chondrocytes did not. When the attachment characteristics of both types of chondrocytes were compared, tumor chondrocytes required fibronectin for attachment, while normal chondrocytes used another attachment factor that had been identified previously as chondronectin. These studies suggest that, although biochemically similar to normal chondrocytes, tumor chondrocytes are no longer able to express the regulatory mechanisms for fibronectin accumulation. [less ▲]

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See detailSerum CBG, free and total cortisol and circadian patterns of adrenal function in normal pregnancy
Demey-Ponsart, E.; Foidart, Jean-Michel ULg; Sulon, J. et al

in Journal of Steroid Biochemistry (1982), 16(2), 165-9

Parameters of cortisol metabolism were studied in pregnancy and early post partum and in non pregnant women. Total serum cortisol was measured by a specific radioimmunoassay and its unbound- and CBG-bound ... [more ▼]

Parameters of cortisol metabolism were studied in pregnancy and early post partum and in non pregnant women. Total serum cortisol was measured by a specific radioimmunoassay and its unbound- and CBG-bound fractions by equilibrium dialysis at 37 degrees C. CBG was measured directly, by an immunodiffusion method (true CBG) and indirectly, by the Scatchard analysis of its interaction with labelled cortisol (PT). During pregnancy the increases in true CBG and total cortisol concentrations were grossly parallel but the PT/true CBG ratio fell from almost 1 in non-pregnant and delivered women to 0.6 during the second half of pregnancy. At 6 p.m., unbound cortisol was significantly higher in outpatients during the 2d half of pregnancy than in non pregnant controls (0.017 vs 0.009 10(-6) mol/l). In hospitalized resting patients, unbound cortisol concentrations were the same in pregnant and non pregnant women except for a significantly higher level of unbound cortisol at midnight during the second half of pregnancy. Circadian variations of cortisol secretion persisted throughout pregnancy. It is concluded that in pregnancy (1) hypothalamic control of maternal adrenal secretion still prevails, (2) the increase in total cortisol is essentially due to that of CBG, (3) the unbound cortisol is normal or, at times, slightly increased and (4) variations in the PT/true CBG ratios may be explained by the presence of steroids (progesterone and 17-OH-progesterone) with a high affinity for CBG. The physiological significance of the mild hypercortisolism of pregnancy is discussed. [less ▲]

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See detailEffets du cis-dichlorodiammineplatine (II) (cis-Pt II) sur l'ultrastructure de la chromatine de cellules animales ou vegetales et repercussions sur le cycle cellulaire.
De Pauw, Marie-Claire ULg; Heinen, Ernst ULg; Houssier, Claude ULg et al

in Comptes Rendus des Séances de la Société de Biologie et de ses Filiales (1982), 176(3), 364-8

Cultivated animal cells (mouse peritoneal macrophages, chick fibroblasts or mouse Ehrlich tumor cells) or Zea mays root tips are treated with cis-Pt (II). Various effects (chromatin dispersion or ... [more ▼]

Cultivated animal cells (mouse peritoneal macrophages, chick fibroblasts or mouse Ehrlich tumor cells) or Zea mays root tips are treated with cis-Pt (II). Various effects (chromatin dispersion or condensation, pycnosis) are observed under some experimental conditions in all cell types. Cytoplasmic ribosomes in helical aggregates appear but only in vegetal cells. Mitosis and cell cycle disturbances due to cis-Pt (II) are probably related to chromatin alterations. We suggest that the latter and helical polyribosomes are produced by cis-Pt (II) reaction with nucleic acids in these structures. [less ▲]

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See detailMercuric chloride induced autoimmune disease in Brown-Norway rats: sequential search for anti-basement membrane antibodies and circulating immune complexes.
Bellon, B.; Capron, M.; Druet, E. et al

in European Journal of Clinical Investigation (1982), 12(2), 127-33

Mercuric chloride induces in the Brown-Norway rat a biphasic autoimmune disease characterized initially by linear IgG deposits along the glomerular basement membrane followed later by granular IgG ... [more ▼]

Mercuric chloride induces in the Brown-Norway rat a biphasic autoimmune disease characterized initially by linear IgG deposits along the glomerular basement membrane followed later by granular IgG deposition. In the present study, anti-glomerular basement membrane antibodies and immune complex-like material were sequentially assessed in serial serum samples. Both were transiently found at the same period. Glomerular linear IgG deposits were present on day 11 but circulating anti-glomerular basement membrane antibodies were only found later on day 16. Circulating immune complexes were first detectable on day 8 before the earliest granular IgG deposits were first observed in the spleen vessels on day 16. The disappearance of circulating anti-glomerular basement membrane antibodies and of circulating immune complexes, although HgCl2 injections were pursued, is in agreement with the self-limited character of mercuric chloride induced autoimmune disease and suggests the induction of immunosuppressive mechanisms. [less ▲]

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See detailAntibodies to laminin in Chagas' disease
Szarfman, A.; Terranova, V. P.; Rennard, S. I. et al

in Journal of Experimental Medicine (1982), 155(4), 1161-71

We have found that sera from humans with Chagas' disease and Rhesus monkeys infected with Trypanosoma cruzi contain IgM and IgG antibodies, which react with structures in a variety of connective tissues ... [more ▼]

We have found that sera from humans with Chagas' disease and Rhesus monkeys infected with Trypanosoma cruzi contain IgM and IgG antibodies, which react with structures in a variety of connective tissues. These antibodies react with laminin but not with various other purified connective tissue components like collagen types I, III, IV, and V, fibronectin, heparan sulfate (BM-1) proteoglycan, or chondronectin. The tissue-reacting antibodies were isolated by absorption to a laminin-Sepharose column. The bound fraction contained all the tissue-reacting antibodies. These antibodies strongly stained trypomastigotes and amastigotes, but weakly stained epimastigotes. These studies show that sera from T. cruzi-infected primates contain antilaminin antibodies, which may be produced by those host in response to a laminin-like molecule present in the parasite. [less ▲]

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See detail8) Continuous Fermentation : Improvement of Cell Immobilization by Zeta Potential Measurement.
Michaux, M.; Paquot, Michel ULg; Baijot, B. et al

in Biotechnol. Bioeng. Symp., 12 (1982)

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