Evidence for a particular binding capacity of rat peritoneal macrophages to rat glomerular mesangial cells in vitro.
; ; et al
in European Journal of Clinical Investigation (1982), 12(3), 239-46
The adhesion of normal rat peritoneal macrophages to normal rat glomerular epithelial or mesangial cells has been studied in vitro after a 60 min incubation at 37 degree C. After washing, the cell ... [more ▼]
The adhesion of normal rat peritoneal macrophages to normal rat glomerular epithelial or mesangial cells has been studied in vitro after a 60 min incubation at 37 degree C. After washing, the cell preparations were examined by phase contrast or scanning electron microscopy. Quantitative studies were also performed using macrophages labelled with 99mTc tin colloids. Peritoneal macrophages predominantly adhered to the cultured mesangial cells. The percent-age of labelled macrophages adhering to these cells was about 10 times higher than that of labelled macrophages adhering to the cultured epithelial cells. This percentage increased proportionally to the number of labelled macrophages added, and was strongly reduced by the prior incubation of macrophagic cells with aggregated IgG, with anti-fibronectin IgG, or with F(ab')2 fragments of anti-fibronectin IgG. Furthermore, the macrophage-mesangial cell interaction was significantly reduced by the prior incubation of mesangial cells with anti-fibronectin IgG or with F(ab')2 fragments of anti-fibronectin IgG. The data demonstrate that normal rat peritoneal macrophages preferentially adhere in vitro to normal rat glomerular mesangial cells, and that this binding may be modulated, at least, by: (a) the Fc receptor binding activity of macrophages; (b) the fibronectin molecules available at the surface of macrophages and of mesangial cells. [less ▲]Detailed reference viewed: 9 (0 ULg)
Immunofluorescence localization of fibronectin in chondrosarcoma cartilage matrix.
; Foidart, Jean-Michel ; et al
in Cancer Research (1982), 42(6), 2384-91
In this study, we have compared the extracellular matrix components and the in vitro adhesion characteristics of normal rat epiphysial chondrocytes with those from the Swarm rat chondrosarcoma, which has ... [more ▼]
In this study, we have compared the extracellular matrix components and the in vitro adhesion characteristics of normal rat epiphysial chondrocytes with those from the Swarm rat chondrosarcoma, which has many of the biochemical characteristics of normal cartilage. With the use of immunofluorescence techniques, tissue slices and chondrocytes in culture were tested for the presence of collagen types I and II, cartilage-characteristic proteoglycan, and fibronectin. Both normal and tumor matrix contained type II collagen and cartilage proteoglycan, but only the tumor matrix contained fibronectin. In culture, tumor-derived chondrocytes continued to accumulate fibronectin in their matrix, even after deposition of type II collagen and proteoglycans, while normal chondrocytes did not. When the attachment characteristics of both types of chondrocytes were compared, tumor chondrocytes required fibronectin for attachment, while normal chondrocytes used another attachment factor that had been identified previously as chondronectin. These studies suggest that, although biochemically similar to normal chondrocytes, tumor chondrocytes are no longer able to express the regulatory mechanisms for fibronectin accumulation. [less ▲]Detailed reference viewed: 10 (0 ULg)
Serum CBG, free and total cortisol and circadian patterns of adrenal function in normal pregnancy
; Foidart, Jean-Michel ; et al
in Journal of Steroid Biochemistry (1982), 16(2), 165-9
Parameters of cortisol metabolism were studied in pregnancy and early post partum and in non pregnant women. Total serum cortisol was measured by a specific radioimmunoassay and its unbound- and CBG-bound ... [more ▼]
Parameters of cortisol metabolism were studied in pregnancy and early post partum and in non pregnant women. Total serum cortisol was measured by a specific radioimmunoassay and its unbound- and CBG-bound fractions by equilibrium dialysis at 37 degrees C. CBG was measured directly, by an immunodiffusion method (true CBG) and indirectly, by the Scatchard analysis of its interaction with labelled cortisol (PT). During pregnancy the increases in true CBG and total cortisol concentrations were grossly parallel but the PT/true CBG ratio fell from almost 1 in non-pregnant and delivered women to 0.6 during the second half of pregnancy. At 6 p.m., unbound cortisol was significantly higher in outpatients during the 2d half of pregnancy than in non pregnant controls (0.017 vs 0.009 10(-6) mol/l). In hospitalized resting patients, unbound cortisol concentrations were the same in pregnant and non pregnant women except for a significantly higher level of unbound cortisol at midnight during the second half of pregnancy. Circadian variations of cortisol secretion persisted throughout pregnancy. It is concluded that in pregnancy (1) hypothalamic control of maternal adrenal secretion still prevails, (2) the increase in total cortisol is essentially due to that of CBG, (3) the unbound cortisol is normal or, at times, slightly increased and (4) variations in the PT/true CBG ratios may be explained by the presence of steroids (progesterone and 17-OH-progesterone) with a high affinity for CBG. The physiological significance of the mild hypercortisolism of pregnancy is discussed. [less ▲]Detailed reference viewed: 16 (0 ULg)
Effets du cis-dichlorodiammineplatine (II) (cis-Pt II) sur l'ultrastructure de la chromatine de cellules animales ou vegetales et repercussions sur le cycle cellulaire.
De Pauw-Gillet, Marie-Claire ; Heinen, Ernst ; Houssier, Claude et al
in Comptes Rendus des Séances de la Société de Biologie et de ses Filiales (1982), 176(3), 364-8
Cultivated animal cells (mouse peritoneal macrophages, chick fibroblasts or mouse Ehrlich tumor cells) or Zea mays root tips are treated with cis-Pt (II). Various effects (chromatin dispersion or ... [more ▼]
Cultivated animal cells (mouse peritoneal macrophages, chick fibroblasts or mouse Ehrlich tumor cells) or Zea mays root tips are treated with cis-Pt (II). Various effects (chromatin dispersion or condensation, pycnosis) are observed under some experimental conditions in all cell types. Cytoplasmic ribosomes in helical aggregates appear but only in vegetal cells. Mitosis and cell cycle disturbances due to cis-Pt (II) are probably related to chromatin alterations. We suggest that the latter and helical polyribosomes are produced by cis-Pt (II) reaction with nucleic acids in these structures. [less ▲]Detailed reference viewed: 29 (10 ULg)
Mercuric chloride induced autoimmune disease in Brown-Norway rats: sequential search for anti-basement membrane antibodies and circulating immune complexes.
; ; et al
in European Journal of Clinical Investigation (1982), 12(2), 127-33
Mercuric chloride induces in the Brown-Norway rat a biphasic autoimmune disease characterized initially by linear IgG deposits along the glomerular basement membrane followed later by granular IgG ... [more ▼]
Mercuric chloride induces in the Brown-Norway rat a biphasic autoimmune disease characterized initially by linear IgG deposits along the glomerular basement membrane followed later by granular IgG deposition. In the present study, anti-glomerular basement membrane antibodies and immune complex-like material were sequentially assessed in serial serum samples. Both were transiently found at the same period. Glomerular linear IgG deposits were present on day 11 but circulating anti-glomerular basement membrane antibodies were only found later on day 16. Circulating immune complexes were first detectable on day 8 before the earliest granular IgG deposits were first observed in the spleen vessels on day 16. The disappearance of circulating anti-glomerular basement membrane antibodies and of circulating immune complexes, although HgCl2 injections were pursued, is in agreement with the self-limited character of mercuric chloride induced autoimmune disease and suggests the induction of immunosuppressive mechanisms. [less ▲]Detailed reference viewed: 13 (0 ULg)
Antibodies to laminin in Chagas' disease
; ; et al
in Journal of Experimental Medicine (1982), 155(4), 1161-71
We have found that sera from humans with Chagas' disease and Rhesus monkeys infected with Trypanosoma cruzi contain IgM and IgG antibodies, which react with structures in a variety of connective tissues ... [more ▼]
We have found that sera from humans with Chagas' disease and Rhesus monkeys infected with Trypanosoma cruzi contain IgM and IgG antibodies, which react with structures in a variety of connective tissues. These antibodies react with laminin but not with various other purified connective tissue components like collagen types I, III, IV, and V, fibronectin, heparan sulfate (BM-1) proteoglycan, or chondronectin. The tissue-reacting antibodies were isolated by absorption to a laminin-Sepharose column. The bound fraction contained all the tissue-reacting antibodies. These antibodies strongly stained trypomastigotes and amastigotes, but weakly stained epimastigotes. These studies show that sera from T. cruzi-infected primates contain antilaminin antibodies, which may be produced by those host in response to a laminin-like molecule present in the parasite. [less ▲]Detailed reference viewed: 17 (2 ULg)
8) Continuous Fermentation : Improvement of Cell Immobilization by Zeta Potential Measurement.
; Paquot, Michel ; et al
in Biotechnol. Bioeng. Symp., 12 (1982)Detailed reference viewed: 15 (0 ULg)
10) Caractérisation de deux celluloses microcristallines et étude de leur comportement électrocinétique
in Lebensm.-Wiss.u.- Technol. (1982)Detailed reference viewed: 63 (6 ULg)
Les industries agro-alimentaires, la biotechnologie, l'exploitation de la biomasse, pôles de développement pour les années 1985-2000. Partie 3. La biomasse: un patrimoine à gérer.
in Bulletin de la Chambre de Commerce Belgique-Luxembourg-Pays Arabes (1982)Detailed reference viewed: 6 (0 ULg)
Electronic properties of simple metals, electronegativity scales and alloy formation
Magain, Pierre ;
in Physica B: Condensed Matter (1982), 112Detailed reference viewed: 16 (1 ULg)
Density-functional theory of the pressure distribution at metal surfaces
; Magain, Pierre
in Physica Status Solidi B. Basic Research (1982), 110Detailed reference viewed: 5 (2 ULg)
Une nouvelle station française de lichens foliicoles dans le Massif central occidental (Aveyron)
in Cryptogamie. Bryologie, Lichenologie (1982), 3(1), 73-76Detailed reference viewed: 13 (0 ULg)
Quelques aspects d'un cycle annuel du zooplancton en baie de Calvi (Corse)
in Annales de la Société Royale Zoologique de Belgique (1982), 112Detailed reference viewed: 14 (3 ULg)
Effet de l'ozone sur le virus de la rhinotrachéite infectieuse bovine (Bovid herpesvirus 1)
; Thiry, Etienne ; Pastoret, Paul-Pierre et al
in Annales de Médecine Vétérinaire (1982), 126Detailed reference viewed: 17 (0 ULg)
Fréquence de l'infection du pigeon par le Pigeon herpèsivurs 1 et le virus de la maladie de Newcastle dans le nord de la France
; Vindevogel, Henri ; Pastoret, Paul-Pierre et al
in Recueil de Médecine Vétérinaire (1982), 158Detailed reference viewed: 17 (0 ULg)
Mise au point d'un dispositif de mesure de la résistance au roulement de pneumatiques agraires
Doctoral thesis (1982)Detailed reference viewed: 23 (5 ULg)
Transfert radiatif dans des enveloppes en expansion rapide: moment W1 d'un profil de raie de type P Cygni et taux de perte de masse
Master's dissertation (1982)Detailed reference viewed: 11 (4 ULg)
The Dissociative Electroionization of Nitrous Oxide. The N+ and O+ Dissociation Channels.
Locht, Robert ; ;
in Schmid, E. R. (Ed.) Book of Abstracts of the 9th International Mass Spectrometry Conference (1982)
The first results on the dissociative elctroionization of nitrous oxide in the N+ and O+ dissociation channels are reported in the 15-32 eV electron energy range. Dissociation mechanisms at several ... [more ▼]
The first results on the dissociative elctroionization of nitrous oxide in the N+ and O+ dissociation channels are reported in the 15-32 eV electron energy range. Dissociation mechanisms at several critical energies are presented. [less ▲]Detailed reference viewed: 10 (1 ULg)
Les immeubles industriels communs à étages : une solution d'intégration industrielle en milieu urbain ?
in L'usine dans la ville (1982)
Comparative study in different countries of this new formula in industrial locationDetailed reference viewed: 29 (0 ULg)