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See detailEnregistrement de l'activité électrophysiologique des neurones monoaminergiques chez le rat
Scuvée-Moreau, Jacqueline ULg; Dubois, Michel ULg; Dresse, Albert ULg

in Archives Internationales de Physiologie et de Biochimie (1977), LXXXV(3), 603-612

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See detailPhotoelectric light curves of minor planets 599 Luisa and 128 Nemesis during the 1976 opposition
Debehogne, H.; Surdej, Anna ULg; Surdej, Jean ULg

in Astronomy and Astrophysics. Supplement Series (1977), 30

Asteroids 599 Luisa and 128 Nemesis were observed photometrically at the European Southern Observatory during the 1976 opposition. The light curve of 599 Luisa shows two well-shaped minima and maxima ... [more ▼]

Asteroids 599 Luisa and 128 Nemesis were observed photometrically at the European Southern Observatory during the 1976 opposition. The light curve of 599 Luisa shows two well-shaped minima and maxima together with a short-time-scale feature. The synodic period of rotation found for 599 Luisa 9 hr 33 min 58 (+ or -46) sec, and the maximum amplitude of the light curve is 0.18 mag. Minor planet 128 Nemesis could only be observed during one night and appears to be a slowly rotating asteroid. [less ▲]

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See detailIndex du «Corpus hermeticum»
Delatte, Louis; Govaerts, Suzanne; Denooz, Joseph ULg

Book published by Edizioni dell'Ateneo e Bizzarri (1977)

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See detailEtude tridimensionnelle du noyau de fibroblastes en culture in vitro.
Bassleer, R.; Heinen, Ernst ULg

in Archives de Biologie (1977), 88(1), 53-9

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See detailTreatment of gas gangrene. Interest of hyperbaric oxygen therapy.
Blaise, G.; Noel, F.; Lamy, Maurice ULg

in Acta Anaesthesiologica Belgica (1977), 28(1), 41-52

We report 27 cases of gas gangrene treated at the University Hospital of Liege since the Anesthesiology Department has been using a caisson for hyperbaric oxygen therapy. Frequency of gas gangrene does ... [more ▼]

We report 27 cases of gas gangrene treated at the University Hospital of Liege since the Anesthesiology Department has been using a caisson for hyperbaric oxygen therapy. Frequency of gas gangrene does not appear to have decreased during recent years. The etiologies now encountered are mainly of post-traumatic and post-operative origin (especially surgery on the digestive tract); arterial insufficiency is a predisposing factor. The prognosis depends on the speed with which an effectual treatment is started; the latter consists in intensive hyperbaric oxygen therapy, antibiotic therapy and resuscitation. Surgery should be as conservative as possible and is undertaken only when progress of the disease has been checked by hyperbaric oxygen therapy. The association of these various modes of treatment proves effectual despite a still significant mortality. [less ▲]

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See detailRegulation of growth hormone messenger RNA by thyroid and glucocorticoid hormones
Martial, Joseph ULg; Baxter, John D; Goodman, Howard M et al

in Proceedings of the National Academy of Sciences of the United States of America (1977), 74(5), 1816-20

Thyroid and glucocorticoid hormones stimulate growth hormone synthesis in cultured rat pituitary cells (GC). We have compared changes in growth hormone production and mRNA in these cells. Triiodothyronine ... [more ▼]

Thyroid and glucocorticoid hormones stimulate growth hormone synthesis in cultured rat pituitary cells (GC). We have compared changes in growth hormone production and mRNA in these cells. Triiodothyronine (10 nM) and dexamethasone (1 micron) stimulated increases in growth hormone production by 2.5- and 3.8-fold, respectively. There were corresponding increases in the capacity of RNA from hormone-treated cells to direct synthesis of pregrowth hormone in a wheat germ cell-free translation system, suggesting hormone-regulated increases in growth hormone mRNA. Hormone-induced changes in mRNA were also demonstrated by determining the kinetics of hybridization of a cDNA probe prepared from RNA enriched (about 70%) for growth hormone translational activity with RNA from control and hormone-treated cells. These results suggest that thyroid and glucocorticoid hormones can regulate growth hormone production by influencing the levels of its mRNA. [less ▲]

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See detailRegulation of growth hormone gene expression: synergistic effects of thyroid and glucocorticoid hormones
Martial, Joseph ULg; Seeburg, Peter H; Guenzi, Doris et al

in Proceedings of the National Academy of Sciences of the United States of America (1977), 74(10), 4293-4295

Cultured rat pituitary cells (GC) respond to thyroid and glucocorticoid hormones by increases in growth hormone production and growth hormone mRNA. When these cells are transferred from medium containing ... [more ▼]

Cultured rat pituitary cells (GC) respond to thyroid and glucocorticoid hormones by increases in growth hormone production and growth hormone mRNA. When these cells are transferred from medium containing normal animal serum (with 1.8 mug of thyroxine per dl) to a medium containing serum from a thyroidectomized calf, "hypothyroid medium" (with no detectable thyroid hormone), growth hormone production decreases markedly. In cells maintained for 5 days in hypothyroid medium, triiodothyronine induces within 50 hr a 17-fold increase in growth hormone production whereas glucocorticoids, during the same time, produce a negligible (3-fold or less) stimulation. In combination, the two hormones promote a 45-fold stimulation. In all instances the changes in growth hormone production are paralleled by changes in the levels of growth hormone mRNA as measured by cell-free translation. The transfer to hypothyroid medium and the hormonal induction do not affect the relative activities of other mRNAs whose products are detectable on polyacrylamide gels. These studies indicate that thyroid hormone can be an activator of the expression of the growth hormone gene. The results also show that triiodothyronine controls the magnitude of the effect of glucocorticoids on growth hormone mRNA, and provide a model for "permissive" triiodothyronine action. The synergistic effect of these two classes of hormone suggests that they increase levels of growth hormone mRNA by different mechanisms. [less ▲]

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See detailNucleotide sequence and amplification in bacteria of structural gene for rat growth hormone
Seeburg, P. H.; Shine, J.; Martial, Joseph ULg et al

in Nature (1977), 270(5637), 486-94

The primary structure of DNA containing the sequence for rat pituitary growth hormone mRNA has been determined. DNA was obtained by reverse transcription of polyadenylated RNA from cultured pituitary ... [more ▼]

The primary structure of DNA containing the sequence for rat pituitary growth hormone mRNA has been determined. DNA was obtained by reverse transcription of polyadenylated RNA from cultured pituitary cells and from recombinant bacterial plasmids. The amino acid sequences for rat growth hormone and its precursor form have been deduced from the determined nucleotide sequences. [less ▲]

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See detailNucleotide sequence of part of the gene for human chorionic somatomammotropin: purification of DNA complementary to predominant mRNA species
Seeburg, Peter H; Shine, John; Martial, Joseph ULg et al

in Cell (1977), 12(1), 157-65

A novel purification procedure for DNA complementary to individual mRNA species has been developed by using restriction endonuclease cleavage of cDNA transcribed from a complex mixture of mRNAs. This ... [more ▼]

A novel purification procedure for DNA complementary to individual mRNA species has been developed by using restriction endonuclease cleavage of cDNA transcribed from a complex mixture of mRNAs. This procedure has allowed us to isolate and analyze DNA fragments complementary to the mRNA coding for the human peptide hormone chorionic somatomammotropin (HCS). The mRNA for this hormone is a major constituent of placental polyadenylated RNA as shown by in vitro translation of placental RNA and by nucleic acid hybridization using HCS cDNA as a specific probe. The purification of HCS cDNA was achieved by Hae III and Hha I restriction endonuclease cleavage of single-stranded cDNA synthesized in vitro from total polyadenylated placental RNA. Polyacrylamide gel electrophoresis of the products allowed detection and purification of discrete DNA fragments. A comparison of the nucleotide sequence of these fragments with that predicted from the amino acid sequence of HCS demonstrated that the fragments are transcripts of HCS mRNA, containing most of the translated and 3′ untranslated regions. The latter region is characterized by a UAG termination codon immediately adjacent to the translated region (a second in phase UAG occurs 9 nucleotides away) and a palindromic sequence (GUGACCCCUCCCCAGUG) centered 27 nucleotides from the termination codon. The purification scheme outlined for HCS cCNA should be applicable to DNA sequences complementary to mRNA species which represent at least 2% of any polyadenylated RNA preparation. This was demonstrated by restriction endonuclease cleavage of cDNA synthesized from a mixture of purified rabbit globin and total polyadenylated human placental RNAs. [less ▲]

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See detailConstruction and analysis of recombinant DNA for human chorionic somatomammotropin
Shine, J.; Seeburg, P. H.; Martial, Joseph ULg et al

in Nature (1977), 270(5637), 494-499

DNA complementary to mRNA coding for the human polypeptide hormone, chorionic somatomammotropin, has been purified by specific restriction endonuclease digestion and religation before cloning into ... [more ▼]

DNA complementary to mRNA coding for the human polypeptide hormone, chorionic somatomammotropin, has been purified by specific restriction endonuclease digestion and religation before cloning into bacterial plasmids. The primary structure of a major portion of this mRNA species is deduced from the nucleotide sequence of the recombinant DNA. [less ▲]

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See detailTestostérone et sexualité chez l'homme
Legros, Jean-Jacques ULg; Mormont, Christian ULg; Servais, Jean

in Expansion Scientifique Française (1977)

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See detailThe Metastable Décomposition of Methane.
Flamme, J. P.; Wankenne, H.; Locht, Robert ULg et al

in International Conference on the Physics of Electronic and Atomic Collisions: abstract of papers. Volume 1. (1977)

The metastable decomposition of CH(D)4+ into CH(D)3+ and H(D) is investigated in a tandem mass spectrometer. The results are interpreted in terms of rotational predissociation of CH(D)4+. A semi-classical ... [more ▼]

The metastable decomposition of CH(D)4+ into CH(D)3+ and H(D) is investigated in a tandem mass spectrometer. The results are interpreted in terms of rotational predissociation of CH(D)4+. A semi-classical model is developed. [less ▲]

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See detailThe Dissociative Ionization of Carbon Monoxide.
Locht, Robert ULg

in Chemical Physics (1977), 22

The dissociative ionization of CO by low energy electron impact into C+ and O (or O-) and O+ and C (or C-) is investigated using ion energy and mass analysis. The kinetic energy distributions are studied ... [more ▼]

The dissociative ionization of CO by low energy electron impact into C+ and O (or O-) and O+ and C (or C-) is investigated using ion energy and mass analysis. The kinetic energy distributions are studied as a function of the impinging electron energy. The threshold energies of C+ and O+ are measured as a function of the released kinetic energy. The formation of the products in their ground level and with a small amount of kinetic energy mainly occurs through predissociation. The CO+ electronic states involved in these processes are tentatively identified. New evidences have been found for the importance of dissociative ionization via autoionization. [less ▲]

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See detailSimultaneous photometry and spectroscopy of the peculiar B [e] star with infrared excess HD45677
Klutz, M.; Surdej, Anna ULg; Surdej, Jean ULg et al

in Kippenhahn, R.; Rahe, J.; Strohmeier, W. (Eds.) THE INTERACTION OF VARIABLE STARS WITH THEIR ENVIRONMENT Proceedings of IAU Colloquium 42 (1977)

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See detailPerception de l'espace et quartiers urbains
Merenne-Schoumaker, Bernadette ULg

in G.E.O. (1977), (2), 33-34

Theoretical mechanisms of perception of space and study areas perceived and experienced

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See detailStructural basis of hemadsorbing sites during the formation of syncytia in measles-infected cells
Rentier, Bernard ULg; Dubois-Dalcq, Monique

in Journal of Cell Biology (1977), 75

The surfaces of cells Infected with measles virus adsorb monkey red blood cells (RBC). In this study, the structure and localization of viral hemadsorbing (HAD) sites and their relationship with antigenic ... [more ▼]

The surfaces of cells Infected with measles virus adsorb monkey red blood cells (RBC). In this study, the structure and localization of viral hemadsorbing (HAD) sites and their relationship with antigenic sites and with cell fusion were investigated in measles virus infected Vero cells. Transmission and scanning electron microscopy were combined with immunolabeling, using human anti-measles IgG (Ab) and protein A from Staph, aureus coupled to peroxidase.. In the early syncytia (50-100 2 in diameter), HAD sites were clustered in the center but scattered at the periphery. In contrast, mature giant cells (400 to 500 2) had all HAD sites in the central area which displayed scattered villi. The periphery of the mature giant cells and the mononucleated cells did not hemadsorb but were covered with numerous villi. In the central area, RBC were firmly attached to villi and ridges over nucleocapsids but rarely to viral buds. Villi and ridges under the RBC were covered with antigenic sites which were not detected at the periphery of the mature syncytia. When living cells were treated with Ab at 4°C, complete inhibition of hemadsorption was only observed when RBC were applied to the cells in the cold. In other experiments, cells reacted with Ab at 4°C were washed, brought to 37°C and treated with RBC immediately or after 1-24 hrs. After 1-2 hrs, some HAD sites were present only at the periphery of immature giant cells, suggesting that RBC receptors had already emerged in membrane areas where fusion started again. After 24 hrs, the distribution of HAD and antigenic sites was the same as on cells not exposed to Ab. It seems that when fusion begins, HAD sites appeared on the periphery of the syncytia and then move spontaneously towards the center. With cessation of fusion, HAD sites disappear from the periphery of the giant cell. Receptors for RBC are closely associated with antigenic sites and correlated with viral induced fusion but not with virus production. [less ▲]

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