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See detailStructural basis of hemadsorbing sites during the formation of syncytia in measles-infected cells
Rentier, Bernard ULiege; Dubois-Dalcq, Monique

in Journal of Cell Biology (1977), 75

The surfaces of cells Infected with measles virus adsorb monkey red blood cells (RBC). In this study, the structure and localization of viral hemadsorbing (HAD) sites and their relationship with antigenic ... [more ▼]

The surfaces of cells Infected with measles virus adsorb monkey red blood cells (RBC). In this study, the structure and localization of viral hemadsorbing (HAD) sites and their relationship with antigenic sites and with cell fusion were investigated in measles virus infected Vero cells. Transmission and scanning electron microscopy were combined with immunolabeling, using human anti-measles IgG (Ab) and protein A from Staph, aureus coupled to peroxidase.. In the early syncytia (50-100 2 in diameter), HAD sites were clustered in the center but scattered at the periphery. In contrast, mature giant cells (400 to 500 2) had all HAD sites in the central area which displayed scattered villi. The periphery of the mature giant cells and the mononucleated cells did not hemadsorb but were covered with numerous villi. In the central area, RBC were firmly attached to villi and ridges over nucleocapsids but rarely to viral buds. Villi and ridges under the RBC were covered with antigenic sites which were not detected at the periphery of the mature syncytia. When living cells were treated with Ab at 4°C, complete inhibition of hemadsorption was only observed when RBC were applied to the cells in the cold. In other experiments, cells reacted with Ab at 4°C were washed, brought to 37°C and treated with RBC immediately or after 1-24 hrs. After 1-2 hrs, some HAD sites were present only at the periphery of immature giant cells, suggesting that RBC receptors had already emerged in membrane areas where fusion started again. After 24 hrs, the distribution of HAD and antigenic sites was the same as on cells not exposed to Ab. It seems that when fusion begins, HAD sites appeared on the periphery of the syncytia and then move spontaneously towards the center. With cessation of fusion, HAD sites disappear from the periphery of the giant cell. Receptors for RBC are closely associated with antigenic sites and correlated with viral induced fusion but not with virus production. [less ▲]

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See detailLexique français-turc / turc-français
Marcillet-Jaubert, J.; Tunca, Önhan ULiege

in Dictionnaire illustré multilingue de l'architecture du Proche Orient ancien (1977)

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See detailCaractéristiques des bateaux et des canaux adaptés à l'évolution de la navigation intérieure
Marchal, Jean ULiege

in Bulletin de l'Association Technique Maritime et Française (1977)

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See detailContributions théorique et expérimentale au calcul de la résistance à l'avancement des convois poussés en navigation dans les voies d'eau de sections limitées
Marchal, Jean ULiege

in Annales des Travaux Publics de Belgique = Tijdschrift der Openbare Werken van Belgie (1977), (1 et 2), 77

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See detailBilan des recherches théoriques et expérimentales relatives à la navigation intérieure
Marchal, Jean ULiege

in Bulletin de l’Association Internationale Permanente des Congrès de Navigation [Bulletin de l'AIPCN] (1977), II(27), 31

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See detailEtude du déferlement des ondes, l'onde régulière et le clapotis
Marchal, Jean ULiege; Van Geuns, W.

in Ceres (1977)

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See detailComportement des bateaux poussés lors du passage, du trématage ou du croisement dans un canal rectiligne
Marchal, Jean ULiege; Sronck, R.

in Bulletin de l’Association Internationale Permanente des Congrès de Navigation [Bulletin de l'AIPCN] (1977)

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See detailNucleotide sequence of a human gene coding for a polypeptide hormone
Seeburg, P. H.; Shine, J.; Martial, Joseph ULiege et al

in Transactions of the Association of American Physicians (1977), 90

In summary, a general approach is presented to purify and sequence DNA fragments of a specific gene starting with a heterogeneous mixture of mRNAs. The methodology has been applied to the determination of ... [more ▼]

In summary, a general approach is presented to purify and sequence DNA fragments of a specific gene starting with a heterogeneous mixture of mRNAs. The methodology has been applied to the determination of the DNA sequence of a portion of the gene for human chorionic somatomammotropin. Most of the possible translation codons of the genetic code were found to be used. Some selectivity in the codon choices was found, and this may be important for RNA or gene regulation or structure. The stop codon UAG was found and a second stop codon in the same reading frame was found nine bases farther down. Finally, a "palindrome" sequence was detected in the 3' noncoding region. [less ▲]

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See detailBovine follitropin: Isolation and characterization of the native hormone and its alpha and ß subunits
Beckers, Jean-François ULiege; Closset, Jean ULiege; Maghuin-Rogister, Guy ULiege et al

in Biochimie (1977), 59

1. A reproducible procedure was developed for the purification of bovine follitropin. 2. The methode involved ammonium sulfate precipitation, ion exchange and adsorption chromatography, concanavaline-A ... [more ▼]

1. A reproducible procedure was developed for the purification of bovine follitropin. 2. The methode involved ammonium sulfate precipitation, ion exchange and adsorption chromatography, concanavaline-A-Sepharose chromatography and gel filtration. 3. A specific radioligand receptor assay was used to monitor each chromatographical step. 4. The potency of highly purified bovine follitropin as measured by Steelman and Pohley bioassay was 62 times the NIH-FSH-B1 standard preparation. 5. Contaminations of bovine follitropin by other glycoprotein hormones such as thyrotropin and lutropin amounted to 3 and 0.45 per cent by weight respectively as measured by specific radioimmunoassays and radioligand receptor assays. 6. The subunits alpha and beta of bovine follitropin were obtained by incubation in acidic urea, the chains being then separated by anion exchange chromatography. The subunits were submitted to complete characterization. The amino terminal residue of the alpha subunit is phenylalanine while a half cystine residue was found at the amino-terminal end of the beta chain. 8. Cross-contamination of the alpha and beta subunit preparations was measured by specific radioimmunoassays and amounted to 0.02 and 0.1 per cent by weight respectively. [less ▲]

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See detailTranslation kernels for velocity dependent interactions
Coz, M.; Rochus, Pierre ULiege

in Journal of Mathematical Physics (1977), 18(11), 2232--2240

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See detailThe translation kernel in the $n$-dimensional scattering problem
Coz, Marcel; Rochus, Pierre ULiege

in Journal of Mathematical Physics (1977), 18(11), 2223--2231

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See detailEffects of transfusion on serum iron, serum lactate dehydrogenase and platelets in megaloblastic anemia
Fillet, Georges ULiege; Andrien, J.-M.; Bury, Jean

in American Journal of Clinical Pathology (1977), 68

In 11 patients with megaloblastic anemia, transfusion of packed erythrocytes or washed erythrocytes invariably resulted in a decline in plasma iron concentration to a range of 20-90 µg/dl (3.6 - 16 µmol/l ... [more ▼]

In 11 patients with megaloblastic anemia, transfusion of packed erythrocytes or washed erythrocytes invariably resulted in a decline in plasma iron concentration to a range of 20-90 µg/dl (3.6 - 16 µmol/l) after 36 or 48 hours. The same phenomenon was observed in two of six cases of ineffective erythropoiesis without megaloblastosis and in none of five cases of aplastic anemia. The observed changes did not result from a specific hematinic response or from iron uptake by a non-erythroid compartment. In megaloblastic anemia, alteration in marrow function in response to transfusion was reflected by plasma iron kinetics and serum lactate dehydrogenase values, which indicated marked reductions in both marrow hyperplasia and ineffective erythropoieseis. Transfusion in megaloblastic anemia was also responsible for a 50% reduction in platelet count after 2 to 6 days. The significance of these changes is discussed. [less ▲]

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See detailUse of the Logic 03 for controlled ventilation in hyperbaric oxygen therapy.
Degauque, C.; Lamy, Maurice ULiege; Stas, M.

in Acta Anaesthesiologica Belgica (1977), 28(4), 251-9

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See detailReport of an incident during the use of the Servo-ventilator 900 in paediatric anesthesia.
Degauque, C.; Philippart, C.; Lamy, Maurice ULiege

in Acta Anaesthesiologica Belgica (1977), 28(3), 199-207

The authors report the occurrence of an incident during the use of the Servo-ventilator 900 for paediatric anesthesia; it consisted in self-oscillation of the gas column contained in the patient ... [more ▼]

The authors report the occurrence of an incident during the use of the Servo-ventilator 900 for paediatric anesthesia; it consisted in self-oscillation of the gas column contained in the patient respiratory circuit. After having analysed the incident and outlined the various ways of avoiding it, attention is drawn to the importance of the total circuit volume of the respirator. The respiratory circuit chosen for ventilating children, has a compliance of 1.3 ml/cm H2O. [less ▲]

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See detailNouvelle méthode de détermination de la structure glycéridique
Wathelet, Jean-Paul ULiege; Séverin, Michel; Ledieu, Jacques et al

in Revue Française des Corps Gras (1977), 4

Laméthocle tle calcul de la structure glycéridique décrite par DEROANNE esr améliorée en ajustant, par itérations successives, la distribution de VAN DER WAL, non seulement avec les coefficients obtenus ... [more ▼]

Laméthocle tle calcul de la structure glycéridique décrite par DEROANNE esr améliorée en ajustant, par itérations successives, la distribution de VAN DER WAL, non seulement avec les coefficients obtenus après dosage des triglycérides par chromatographie sur chouche mince imprëgnée de nitrate d'argent, mais également ovec les coe.fficients calculés après sëparation des tiglvcérides par chromatographie en phase gazeuse. Un programme en Fortran IV est mis au point. [less ▲]

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See detailAstronomical photographic atlas
Heck, Andre; Manfroid, Jean ULiege

Book published by Desoer (1977)

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See detailThyroid hormonelike actions of 3,3',5'-L-triiodothyronine nad 3,3'-diiodothyronine
Papavasiliou, S. S.; Martial, Joseph ULiege; Latham, K. R. et al

in Journal of Clinical Investigation (1977), 60(6), 1230-9

l-Thyroxine is converted to 3,5,3'-l-triiodothyronine (T(3)) as well as to 3,3',5'-l-triiodothyronine (reverse T(3)). One product of further deiodination is 3,3'-diiodothyronine (3,3'T(2)). The serum ... [more ▼]

l-Thyroxine is converted to 3,5,3'-l-triiodothyronine (T(3)) as well as to 3,3',5'-l-triiodothyronine (reverse T(3)). One product of further deiodination is 3,3'-diiodothyronine (3,3'T(2)). The serum levels of reverse T(3) and 3,3'T(2) change considerably in various physiological and disease states. We previously found that reverse T(3) and 3,3'T(2) bind to the solubilized hepatic nuclear "receptors" for thyroid hormones. This led us to study binding and actions of these metabolites in cultured rat pituitary cells in which glucose consumption and growth hormone production are regulated by T(3) and l-thyroxine.Reverse T(3) and 3,3'T(2) stimulated growth hormone production and glucose consumption and inhibited nuclear binding of radioactive T(3). Either metabolite produced maximal effects that equaled those of T(3), and neither inhibited the T(3) response. Further, additive effects were observed when reverse T(3) was combined with submaximal concentrations of T(3).In serum-free and serum-containing media, concentrations of 3,3'T(2) 50- to 70- and 10- to 100-fold greater, respectively, than those of T(3) were required for equivalent stimulations and for inhibition of nuclear binding by T(3). The relative activity differences under the two conditions can be attributed to weaker serum protein binding of 3,3'T(2) than T(3). With cells in serum-free media, reverse T(3) was a less avid competitor than 3,3'T(2) for T(3) binding by the nuclear receptors, and was less potent than 3,3'T(2) (0.001 the potency of T(3)) in inducing growth hormone production or glucose oxidation. In incubations with serum-containing media, reverse T(3) was an ineffective competitor for T(3) binding, and had only 0.1 the inducing potency of 3,3'T(2) (0.001 the potency of T(3)). The weaker activity of reverse T(3) relative to 3,3'T(2) in serum-containing media could be explained by stronger serum binding of reverse T(3) than 3,3'T(2). In addition, after long-term incubation of cells with radioactive reverse T(3), much of the cell-associated radioactivity was recovered as 3,3'T(2).These studies suggest that reverse T(3) and 3,3'T(2) can stimulate thyroid hormone-regulated functions as weak agonists by acting via the same receptors that mediate T(3) actions. Moreover, some of the effects of reverse T(3) may be due to 3,3'T(2) produced by deiodination of reverse T(3). [less ▲]

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See detailPhotometry of HR 3413.
Renson, Pierre ULiege; Sterken, C.

in IAU Archives of unpublished observations of variable stars file number :, 55, 1 (1977) (1977), 55

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See detailHeterology and homology between the ribonucleic acid species of plant viruses as evaluated by molecular hybridizations.
Kummert, Jean; Demeure, M.; Portetelle, Daniel ULiege et al

in Biochemical Society Transactions (1977), 5(4), 946-947

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