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See detailExocellular β-lactamases of Streptomyces albus G and strains R39 and K11
Johnson, Kenneth; Dusart, Jean; Campbell, James N. et al

in Antimicrobial Agents and Chemotherapy (1973), 3(2), 289-298

The beta-lactamases excreted by the highly benzylpenicillin-susceptible Streptomyces strain R39 and the highly benzylpenicillin-resistant Streptomyces albus G were isolated and purified. Neither beta ... [more ▼]

The beta-lactamases excreted by the highly benzylpenicillin-susceptible Streptomyces strain R39 and the highly benzylpenicillin-resistant Streptomyces albus G were isolated and purified. Neither beta-lactamase exhibited dd-carboxypeptidase activity. Both were anionic at pH 8.3, did not require metal ions, and were not sensitive to iodine, but were inhibited by Cu(2+) and readily inactivated by heat. p-Chloromercuribenzoate, iodoacetate, p-aminobenzoate, and substrates and inhibitors of dd-carboxypeptidase had no effect on beta-lactamase activity. The K(m) and V(max) values for beta-lactamase activity were studied with 6-aminopenicillanic acid and with various penicillins and cephalosporins. The beta-lactamase from the related strain K11 of Streptomyces, which is intermediate in its susceptibility to benzylpenicillin, was partially purified, and its activity was compared on the various substrates. [less ▲]

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See detailOrganisation d'une bibliothèque de programmes statistiques pour ordinateur : exemples
Carletti, G.; Claustriaux, Jean-Jacques ULg; Dagnelie, P. et al

in Revue Belge de Statistique, d'Informatique et de Recherche Opérationnelle = Belgisch Tijdschrift voor Statistiek, Informatica en Operationeel Onderzoek (1973), 12(4), 2-16

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See detailPeptide inhibitors of Streptomyces DD-carboxypeptidases
Nieto, Manuel; Perkins, Harnold R.; Leyh-Bouille, Mélina et al

in Biochemical Journal (1973), 131(1), 163-171

1. Peptides that inhibit the dd-carboxypeptidases from Streptomyces strains albus G and R61 were synthesized. They are close analogues of the substrates of these enzymes. The enzymes from albus G and R61 ... [more ▼]

1. Peptides that inhibit the dd-carboxypeptidases from Streptomyces strains albus G and R61 were synthesized. They are close analogues of the substrates of these enzymes. The enzymes from albus G and R61 strains are in general inhibited by the same peptides, but the enzyme from strain R39 differs considerably. 2. The two C-terminal residues of the peptide substrates and inhibitors appear to be mainly responsible for the initial binding of the substrate to the enzymes from albus G and R61 strains. The side chain in the third residue from the C-terminus seems critical in inducing catalytic activity. 3. Experimental evidence is presented suggesting that the amide bond linking the two C-terminal residues has a cis configuration when bound to the enzymes from strains albus G and R61. 4. The peptide inhibitors are not antibiotics against the same micro-organisms. [less ▲]

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See detailLe test de closure: mesure de la lisibilité et de la compréhension
De Landsheere, Gilbert ULg

Book published by Labor (1973)

This book describes the cloze test, an instructional tool that has been used in the United States for the past 15 years but has not yet been extensively researched in francophone countries. The first ... [more ▼]

This book describes the cloze test, an instructional tool that has been used in the United States for the past 15 years but has not yet been extensively researched in francophone countries. The first section presents a synthesis of the most important American studies and outlines a theoretical framework for researchers and teachers of French who do not yet use cloze tests. This section includes a definition and history of the cloze test; a description of the test as a comprehension measure, as a diagnostic measure, and as an instrument of linguistic research and instruction; a discussion of the validity of the technique from the theoretical and experimental point of view; and examples of its application. Section 2 reports on exploratory research with Belgian populations ranging in age from 9 to 18. A final section reports that this study confirmed conclusions of American research, i.e., that the cloze test is an economical instrument which provides a valid direct measure of readability and comprehension; and that the test is an important diagnostic, research, and instructional tool. Appendices include the six texts chosen for the test and a test sample. A bibliography completes the volume. (Author/AM) [less ▲]

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See detailCorrélations entre divers paramètres anthropométriques, l'eau corporelle et le potassium total
Delwaide, P. A.; Crenier, E. J.; Albert, Adelin ULg

in Biométrie Humaine (1973), 8

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See detailStructure of the wall peptidoglycan of streptomyces R39 and the specificity profile of its exocellular DD-carboxypeptidase-transpeptidase for peptide acceptors
Ghuysen, Jean-Marie ULg; Leyh-Bouille, Mélina; Campbell, James N. et al

in Biochemistry (1973), 12(7), 1243-1251

Benzylpenicillin and cephaloridine reacted with the exocellular dd-carboxypeptidase–transpeptidase from Streptomyces R39 to form equimolar and inactive antibiotic–enzyme complexes. At saturation, the ... [more ▼]

Benzylpenicillin and cephaloridine reacted with the exocellular dd-carboxypeptidase–transpeptidase from Streptomyces R39 to form equimolar and inactive antibiotic–enzyme complexes. At saturation, the molar ratio of chromogenic cephalosporin 87-312 to enzyme was 1.3:1, but this discrepancy might be due to a lack of accuracy in the measurement of the antibiotic. Spectrophotometric studies showed that binding of cephaloridine and cephalosporin 87-312 to the enzyme caused opening of their β-lactam rings. Benzylpenicillin and cephalosporin 87-312 competed for the same site on the free enzyme, suggesting that binding of benzylpenicillin also resulted in the opening of its β-lactam ring. In Tris–NaCl–MgCl2 buffer at pH7.7 and 37°C, the rate constants for the dissociation of the antibiotic–enzyme complexes were 2.8×10−6, 1.5×10−6 and 0.63×10−6s−1 (half-lives 70, 130 and 300h) for benzylpenicillin, cephalosporin 87-312 and cephaloridine respectively. During the process, the protein underwent reactivation. The enzyme that was regenerated from its complex with benzylpenicillin was as sensitive to fresh benzylpenicillin as the native enzyme. With [14C]benzylpenicillin, the released radioactive compound was neither benzylpenicillin nor benzylpenicilloic acid. The Streptomyces R39 enzyme thus behaved as a β-lactam-antibiotic-destroying enzyme but did not function as a β-lactamase. Incubation at 37°C in 0.01m-phosphate buffer, pH7.0, and in the same buffer supplemented with sodium dodecyl sulphate caused a more rapid reversion of the [14C]benzylpenicillin–enzyme complex. The rate constants were 1.6×10−5s−1 and 0.8×10−4s−1 respectively. Under these conditions, however, there was no concomitant reactivation of the enzyme and the released radioactive compound(s) appeared not to be the same as before. The Streptomyces R39 enzyme and the exocellular dd-carboxypeptidase–transpeptidase from Streptomyces R61 appeared to differ from each other with regard to the topography of their penicillin-binding site. [less ▲]

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See detailExocellular and membrane-bound transpeptidases in Streptomyces
Dusart, Jean; Marquet, A.; Ghuysen, Jean-Marie ULg

in 1st International Congress for Bacteriology, Jerusalem, 2-7 September, 1973. Abstracts. Vol. II. Free papers. (1973)

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See detailThe first long acting "retard" thymoanaleptic or antidepressant: noveril Tr. statistical addendum
Albert, Adelin ULg; Collard, J.; Fraipont, J. et al

in Arzneimittel Forschung (1973), 4

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See detailFotoelektronen Spectroscopie: een nieuwe analyse techniek.
Locht, Robert ULg; Delwiche, Jacques ULg

in Chemisch Weekblad (1973), 69(28/29),

Beside the IR-, Raman- and UV-spectroscopies the photoelectron spectroscopy becomes an important technique for the qualitative and quantitative analysis of unknown samples. Important informations related ... [more ▼]

Beside the IR-, Raman- and UV-spectroscopies the photoelectron spectroscopy becomes an important technique for the qualitative and quantitative analysis of unknown samples. Important informations related to the electronic and molecular structure can be obtained by this technique. [less ▲]

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See detailEtude de la fiabilité du SMA 12/60.
Vos, A.; Ers, P.; Albert, Adelin ULg et al

in Expansion scientifique française (1973)

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See detailMots oubliés du Dictionnaire liégeois de Henri Forir
Droixhe, Daniel ULg

in Musée herstalien (1973)

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See detailModern aspecs of treatment of decompression sickness
Lamy, Maurice ULg; Hanquet, M. M.

in Acta Anaesthesiologica Belgica (1973), 24(3), 215-29

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See detailGamma-glutamylphenylalanine in Dolichos seeds
Dardenne, G. A.; Thonart, Philippe ULg

in Phytochemistry (1973), 12

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See detailUnivers mental et stratégies des évêques : la déclaration sur l'avortement
Mormont, Marc ULg; Mougenot, Catherine ULg; Rucquoy, Danielle

in Social Compass (1973), 10

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See detailApplications de programmes d’ordinateur aux textes relatifs aux ousiai : méthode et traitement
Denooz, Joseph ULg

in Actes du XIIIe Congrès international de papyrologie (1973)

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See detailUltraviolet observations of equatorial dayglow above the F[SUB]2[/SUB] peak.
Boksenberg, A.; Gérard, Jean-Claude ULg

in Journal of Geophysical Research (1973), 78

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See detailTraitement automatique de papyrus grecs
Tomsin, Alfred; Évrard, Étienne; Denooz, Joseph ULg et al

Book published by LASLA (1973)

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See detailRecherches sur le traitement automatique de la langue latine
Denooz, Joseph ULg

in Revue de l'Organisation Internationale pour l'Etude des Langues Anciennes par Ordinateur (1973), (1), 1-89

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See detailPlissements superposés à Belle-Isle-en-Mer (Morbihan)
Darimont, Anne ULg

in Annales de la Société Géologique de Belgique (1973), 96

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