References of "Willems, Luc"
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See detailProtective Effects Of A Live Attenuated Bovine Leukaemia Virus Vaccine With Deletion In The R3 And G4 Genes
Reichert, M.; Cantor, Gh.; Willems, Luc ULg et al

in Journal of General Virology (2000), 81

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See detailBovine leukemia virus as a model for human T-cell leukemia virus
Willems, Luc ULg; Burny, A.; Dangoisse, O. et al

in Current Topics in Virology (1999)

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See detailBovine Leukemia Virus-Induced Persistent Lymphocytosis In Cattle Does Not Correlate With Increased Ex Vivo Survival Of B Lymphocytes
Dequiedt, Franck ULg; Cantor, Gh.; Hamilton, Vt. et al

in Journal of Virology (1999), 73(2),

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See detailLeukemia Viruses
Burny, A.; Bex, F.; Dequiedt, Franck ULg et al

in Encyclopedia of Immunology (1998)

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See detailConservative Mutations In The Immunosuppressive Region Of The Bovine Leukemia Virus Transmembrane Protein Affect Fusion But Not Infectivity In Vivo
GATOT, Jean-Stéphane ULg; Callebaut, I.; Mornon, Jp. et al

in Journal of Biological Chemistry (1998), 273(21),

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See detailIn Vitro And In Vivo Oncogenic Potential Of Bovine Leukemia Virus G4 Protein
Kerkhofs, P.; Heremans, H.; Burny, A. et al

in Journal of Virology (1998), 72(3),

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See detailPhosphorylation of bovine leukemia virus Tax protein is required for in vitro transformation but not for transactivation.
Willems, Luc ULg; Grimonpont, Cathy; Kerkhofs, Pierre et al

in Oncogene (1998), 16(17), 2165-76

The Tax proteins of the oncovirinae viruses are phosphorylated transcriptional activators that exhibit oncogenic potential. The role of phosphorylation in their functional activities remains unknown. As a ... [more ▼]

The Tax proteins of the oncovirinae viruses are phosphorylated transcriptional activators that exhibit oncogenic potential. The role of phosphorylation in their functional activities remains unknown. As a model for the Human T-cell leukemia virus type I (HTLV-I), Bovine Leukemia Virus (BLV) permits the characterization of viral replication and leukemogenesis in vivo. Here, we show that the BLV Tax protein is phosphorylated on serine residues 106 and 293 both in insect and in mammalian cells. These sites can also be efficiently phosphorylated by the cdc2 and MAP kinases in vitro. Mutation of these residues does not affect the capacity of the Tax protein to function as a transactivator. Indeed, the Tax proteins mutated at one or both serines increase LTR-directed viral transcription at levels similar to those obtained with wild-type Tax in cell culture. Moreover, inhibition of Tax phosphorylation by W7, a calmodulin antagonist, does not alter its transactivation activity. Thus, phosphorylation on serines 106 and 293 is not required for transactivation by Tax. However, simultaneous substitution of both serines into alanine residues destroys the capacity of Tax to cooperate with the Ha-ras oncogene to transform primary rat embryo fibroblasts and induce tumors in nude mice. When the serines were replaced with aspartic acid residues, the oncogenic potential of Tax was maintained indicating that the negative charge rather than the phosphate group itself was required for Tax oncogenicity. Finally, to assess the role of the serine residues in vivo, recombinant viruses which express the Tax mutants were constructed and injected into sheep. It appeared that the mutated proviruses replicate at levels similar to the wild-type virus in vivo. We conclude that Tax phosphorylation is dispensable for transactivation and viral replication in vivo but is required for its oncogenic potential in vitro. [less ▲]

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See detailMutations in the immunosuppressive peptide of bovine leukema virus affect fusion and infectivity in vivo
GATOT, Jean-Stéphane ULg; Kettmann, Richard ULg; Callebaut-Mornon, Isabelle et al

in Virus Research (1997), 47(2-Special Issue), 103

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See detailThe Cloning And Sequencing Of An Ovine C-Myc Cdna
Kiermer, V.; Dequiedt, Franck ULg; Masengo, R. et al

in DNA Sequence : The Journal of DNA Sequencing & Mapping (1997), 7(3-4),

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See detailThe Major Homology Region Of Bovine Leukaemia Virus P24(Gag) Is Required For Virus Infectivity In Vivo
Willems, Luc ULg; Kerkhofs, P.; Attenelle, L. et al

in Journal of General Virology (1997), 78

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See detailPhosphorylation of the Bovine Leukemia Virus transactivator p34Tax is required for transformation but not for transactivation.
Kettmann, Richard ULg; Grimonpont, C.; Kerkhofs, Pierre et al

in FASEB Journal (1997), 11(9), 1179

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See detailBovine Herpesvirus 1-Induced Apoptosis Occurs At The G0/G1 Phase Of The Cell Cycle
Hanon, Emilien ULg; Hoornaert, S.; Dequiedt, Franck ULg et al

in Virology (1997), 232(2), 351-358

We have previously shown that bovine herpesvirus 1 (BHV-1), even when inactivated, induces apoptotic cell death in mitogen-stimulated bovine peripheral blood mononuclear cells (PBMCs) (Hanon et al., 1996 ... [more ▼]

We have previously shown that bovine herpesvirus 1 (BHV-1), even when inactivated, induces apoptotic cell death in mitogen-stimulated bovine peripheral blood mononuclear cells (PBMCs) (Hanon et al., 1996, J. Virol. 70, 4116-4120). In order to gain insight into this process, we have investigated the cell cycle phase at which BHV-1 induces apoptosis in PBMCs. Our results show that the percentage of cells that progress through the S phase was always lower in BHV-1-infected PBMCs than in control cells. This effect was not due to a defective activation of mitogen-stimulated PBMCs since BHV-1 only slightly affected the percentage of cells expressing BoCD25, a well-known lymphocyte activation marker. Furthermore, mimosine and cyclosporine A, two chemicals that inhibit entry into the S phase of the cell cycle by different pathways, did not affect the ability of BHV-1 to induce apoptosis. BHV-1-induced apoptosis also occurred in unstimulated PBMCs and interestingly, this was associated with the expression of c-myc and BoCD25 proteins both of which are related to cell cycle progression. All together, these data provide evidence demonstrating that BHV-1-induced apoptosis occurs at the G0/G1 phase of the cell cycle. [less ▲]

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See detailOncogenic potential of Tax requires phosphorylation on serines 106 et 293.
Willems, Luc ULg; Grimonpont, Catherine; Kerkhofs, Pierre et al

in Virus Research (1997), 47(2 -Special Issue), 121-122

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See detailLack Of Mutation In The Waf1/Cip1 Gene During Bovine Leukemia Virus-Induced Leukemogenesis
Dequiedt, Franck ULg; Willems, Luc ULg; Kerkhofs, P. et al

in Veterinary Immunology and Immunopathology (1997), 59(3-4),

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See detailModulation Of Cd3-Gamma Gene Expression After Hiv Type 1 Infection Of The We17/10 T Cell Line Is Progressive And Occurs In Concert With Decreased Production Of Viral P24 Antigen
Willardgallo, Ke.; Delmellewibaut, C.; Segurazapata, I. et al

in Aids Research and Human Retroviruses (1996), 12(8),

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See detailCellular Pathways Involved In The Ex Vivo Expression Of Bovine Leukemia Virus
Kerkhofs, P.; Adam, E.; Droogmans, L. et al

in Journal of Virology (1996), 70(4),

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See detailThe Creb, Atf-1, And Atf-2 Transcription Factors From Bovine Leukemia Virus-Infected B Lymphocytes Activate Viral Expression
Adam, E.; Kerkhofs, P.; Mammerickx, M. et al

in Journal of Virology (1996), 70(3),

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See detailLack of LTR and ENV genetic variation during bovine leukemia virus-induced leukemogenesis.
Willems, Luc ULg; Kerkhofs, P.; Burny, A. et al

in Virology (1995), 206(1),

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