References of "Thonart, Philippe"
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See detailPeptidomic comparison and characterization of the major components of the venom of the giant ant Dinoponera quadriceps collected in four different areas of Brazil.
Cologna, Camila Takeno; Cardoso, Jaqueline Dos Santos; Jourdan, Emmanuel et al

in Journal of Proteomics (2013), 94

Despite the noxious effects inflicted by Dinoponera ant's envenomation, the information about the biological properties and composition of their venom is still very limited. Ants from the genus Dinoponera ... [more ▼]

Despite the noxious effects inflicted by Dinoponera ant's envenomation, the information about the biological properties and composition of their venom is still very limited. Ants from the genus Dinoponera are believed to be the world's largest living ants with a body length of 3cm. Their occurrence is restricted to tropical areas of South America. In this work, we study the venom of the giant Dinoponera quadriceps ant collected in 4 different regions of Brazil. By using a combination of complementary mass spectrometric approaches, we aim at: (i) characterizing the venom composition of these ants; (ii) establishing a comparative analysis of the venom from four geographically different regions in Brazil. This approach demonstrates that ant venom is a copious source of new compounds. Several peptides were identified and selected for "de novo sequencing". Since most of the new peptides showed similarities with antimicrobial peptides (AMPs), antimicrobial assays were performed with the purpose of evaluating their activity. In regard to the comparative study of the four regions, we observed not only major differences in the venom compositions, but also that the venoms collected in closest areas are more similar than the ones collected in distant regions. These observations seem to highlight an adaption of the ant venoms to the local environment. Concerning the biological assays, the peptides called Dq-3162 and Da-3177 showed a wide-ranging antimicrobial activity. The characterization of new AMPs with a broad spectrum of activity and different scaffolds may aid scientists to design new therapeutic agents and understand the mechanisms of those peptides to interact with microbial membranes. The results obtained betoken the biotechnological potential of ant's venom. BIOLOGICAL SIGNIFICANCE: For the first time this manuscript describes an extensive proteomics characterization of the D. quadriceps venom. In addition this study reports the variation in venom composition of primitive ants from 4 geographically different areas of Brazil. The results reveal the presence of ~335 compounds for each venom/area and inter-colony variations were observed. 16 new peptides were characterized and 2 of them were synthesized and biologically assayed. These findings highlight the considerable and still unexplored diversity of ant's venom which could be used as valuable research tools in different areas of knowledge. [less ▲]

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See detailÉtude des interactions entre les transferts liquide/gaz et la physiologie microbienne dans un bioréacteur anaérobie à cuve agitée produisant de l’hydrogène
Baert, Jonathan ULg; de Lamotte, Anne ULg; Crine, Michel ULg et al

Scientific conference (2013, November 15)

Cette thèse de doctorat menée en partenariat avec le Laboratoire de génie chimique (LGC) de l’ULg a comme objectif de caractériser les interactions physico-biologiques durant les bioprocédés visant la ... [more ▼]

Cette thèse de doctorat menée en partenariat avec le Laboratoire de génie chimique (LGC) de l’ULg a comme objectif de caractériser les interactions physico-biologiques durant les bioprocédés visant la production d’hydrogène par fermentation anaérobie en cuve agité. [less ▲]

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See detailWastes of banana ‘s lignocellulosic biomass: a sustainable and renewable source of biogas production
Kamdem, Irenée ULg; Hiligsmann, Serge ULg; Vanderghem, Caroline ULg et al

Poster (2013, November 15)

We highlight in this poster, the results of biogas production and biochemical analysis based on the anaerobic digestion of each type of the lignocellulosic waste from a banana cultivar (Williams Cavendish ... [more ▼]

We highlight in this poster, the results of biogas production and biochemical analysis based on the anaerobic digestion of each type of the lignocellulosic waste from a banana cultivar (Williams Cavendish: triploid Musa AAA group). These wastes are usually abandoned in the plantation after the fruits have been harvested. There is great interest in obtaining energy from this generally neglected biomaterial, particularly in the contexts of global warming and sustainable development. [less ▲]

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See detailCharacterisation of fengycin homologues produced by B. amyloliquefaciens (ET) strain isolated from a salt lake (Eastern Algeria)
Ait Kaki, Asma ULg; Noreddine, K.C.; Kara Ali, M. et al

Poster (2013, November 15)

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See detailUse of on-line flow cytometry for the characterization of physiological behavior in stress conditions during the bioprocess
Brognaux, Alison ULg; Han, Shanshan; Soren, Sorensen et al

Poster (2013, November 15)

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See detailPrésentation des projets Interreg IVB BIOREFINE et RENEW
Tarayre, Cédric ULg; Delvigne, Frank ULg; Destain, Jacqueline ULg et al

Conference (2013, October 22)

Les projets BioRefine et Renew sont financés par le programme de coopération européen Interreg IVB. Leur but est de promouvoir les transferts de compétences, d'informations et de matières entre les ... [more ▼]

Les projets BioRefine et Renew sont financés par le programme de coopération européen Interreg IVB. Leur but est de promouvoir les transferts de compétences, d'informations et de matières entre les différents pays de la zone européenne du Nord-Ouest. Le projet BioRefine a pour but de récupérer les nutriments (N, P et K) ainsi que les éléments traces métalliques à partir de déchets bien spécifiques: le fumier, le lisier, les digestats de méthanisation et les boues de stations d'épuration. Cette récupération requiert l'élaboration de procédés industriels mis en place après une étude en laboratoire. Le projet Renew, lui, est beaucoup plus général. En ce qui concerne le rôle de Gembloux Agro-Bio Tech dans le projet, le travail sera essentiellement focalisé sur la production d'acide succinique à partir de déchets. Cette production sera assurée par la souche bactérienne Actinobacillus succinogenes. [less ▲]

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See detailProjet Termitofuel: les termites et leurs symbiontes pour mieux valoriser la biomasse ligno-cellulosique
Bauwens, Julien ULg; Tarayre, Cédric ULg; Brasseur, Catherine ULg et al

Poster (2013, October 19)

L’exploitation actuelle de la biomasse ligno-cellulosique, extrêmement abondante sur Terre, est liée à la production de déchets relativement peu valorisables. C’est pourquoi le projet TERMITOFUEL étudie ... [more ▼]

L’exploitation actuelle de la biomasse ligno-cellulosique, extrêmement abondante sur Terre, est liée à la production de déchets relativement peu valorisables. C’est pourquoi le projet TERMITOFUEL étudie la digestion du bois chez les termites, grâce à une approche pluridisciplinaire, en vue d’améliorer la production de bioéthanol de seconde génération. Au sein de l’Unité d’Entomologie Fonctionnelle et Evolutive, l’approche protéomique est employée afin d’identifier les micro-organismes présents dans le tube digestif des termites et caractériser les activités enzymatiques. [less ▲]

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See detailUse of on-line flow cytometry to detect segregation in the microbial population
Brognaux, Alison ULg; Zune, Quentin ULg; Han, Shanshan et al

Poster (2013, October 08)

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See detailANAEROBIC DIGESTION OF ORGANIC WASTES FROM AGRO-FOOD ACTIVITIES IN TUNISIA
Mhiri, F; Hiligsmann, Serge ULg; Sayahi, L. et al

Poster (2013, October 01)

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See detailMONITORING OF ANAEROBIC DIGESTION IN MSW LANDFILLS IN TUNISIA
Mhiri, F.; Hiligsmann, Serge ULg; Sayahi, L. et al

in Proceedings Sardinia 2013 (2013, October)

The monitoring of two controlled landfills in Tunisia was carried out for about 10 years by CITET and CWBI. Both landfills were managed by classical methodology i.e. disposal, compaction and, if any ... [more ▼]

The monitoring of two controlled landfills in Tunisia was carried out for about 10 years by CITET and CWBI. Both landfills were managed by classical methodology i.e. disposal, compaction and, if any, biogas and leachate extraction and treatment. One of them is located near Tunis and the other near Sousse with a warmer climate. They received more than 6,000 and 350 thousand cubic metre of MSW respectively from the two cities. Solid samples were collected by boring at different depths up to 18 m. They were analysed for dry weight and for total organic carbon, nitrogen and (hemi-) cellulose. The boreholes were equipped with 200 mm perforated PEHD tubes for further biogas and leachate sampling and analyses (COD, VFA, CH4 content, …) at different seasons. The results indicate an efficient mesophilic anaerobic digestion in both landfills. The high dry weight content (70 – 92%) of 13 solid samples collected in Sousse landfill contrasts however with the high leachate production in the Tunis landfill, filling about completely the majority of boreholes. The results of this monitoring confirm the trends formerly described about biological activity and environmental impact of landfills in Tunisia. Moreover a 200 L pilot-scale anaerobic digester was operated for about 3 years with solid wastes from Tunis landfill to assess the evolution of moisture content and organic matter. [less ▲]

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See detailANAEROBIC DIGESTION WITH BIOHYDROGEN PRODUCTION FROM ORGANIC WASTES : STATE-OF-THE ART
Hiligsmann, Serge ULg; Beckers, L.; Hamilton, C. et al

in Proceedings Sardinia 2013 (2013, October)

Hydrogen (H2) is being considered as an ideal and clean energy carrier since the utilization of hydrogen, either via combustion or via fuel cells, results in pure water. However, currently its production ... [more ▼]

Hydrogen (H2) is being considered as an ideal and clean energy carrier since the utilization of hydrogen, either via combustion or via fuel cells, results in pure water. However, currently its production relies mainly (i.e. 95%) on fossil fuels. The recent advances to produce biohydrogen from renewable sources such as biomass and particularly by fermentation of carbohydrate-rich substrates from agriculture and agro-industries appear promising. Such a process called “dark fermentation” enables both organic waste treatment and renewable energy production to be coupled. The soluble metabolites, mainly acetate and butyrate, contained in the spent medium of the dark fermentation bioreactor may be efficiently converted to methane in a second anaerobic digester treating other carbon sources. The paper reviews the state-of-the art on the challenges and prospective applications of dark fermentation as a first step of anaerobic digestion of municipal solid wastes to produce H2 and CH4. [less ▲]

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See detailStudy of the sorption behavior of Hibscus sabdariffa anthocyanins on a macroporous resin
Beye, Cheikh ULg; Souk Tounkara, Lat; Destain, Jacqueline ULg et al

in Journal of Food Process Engineering (2013), 36(5), 579-590

The adsorption of Hibiscus sabdariffa sp. anthocyanins onto a macroporous resin was studied in laboratory experiments. The anthocyanin aqueous extracts obtained from the calyces were used in agitated ... [more ▼]

The adsorption of Hibiscus sabdariffa sp. anthocyanins onto a macroporous resin was studied in laboratory experiments. The anthocyanin aqueous extracts obtained from the calyces were used in agitated batch experiments with a macroporous resin. The adsorption kinetics and isotherms of H. sabdariffa anthocyanins on a selected macroporous resin was studied to determine the parameters that have to be optimized to recover the anthocyanins from aqueous extracts of the calyces. The adsorption kinetic experimental data were fit into a pseudo second order kinetic model, which was then used to determine several parameters such as the contact time necessary to reach equilibrium as well as the maximum adsorption capacity. The best fit for the equilibrium adsorption isotherm experimental data was obtained with the Langmuir isotherm model. Based on qualitative and quantitative interpretation of the experimental data, the resin that was tested here appeared to have good adsorption capacity for the anthocyanins even though the necessary time to reach equilibrium was particularly long. Three aspects of the desorption of anthocyanins from the resin were studied: the speed of release of the anthocyanins (desorption kinetics), the extent of the anthocyanin desorption depending on the adsorption contact time and finally, the influence of the mobile phase's characteristics [less ▲]

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See detailStress microbien lors du processus d’extrapolation: Approche physique et biologique
Lejeune, Annick ULg; Thonart, Philippe ULg; Delvigne, Frank ULg

in Cerevisia : Belgian Journal of Brewing and Biotechnology (2013), 38(3), 89-101

Les micro-organismes sont utilisés dans de nombreux domaines (agro-alimentaire, pharmaceutique, environnemental, énergétique,…) que ce soit pour la production de biomasse ou de métabolites particuliers ... [more ▼]

Les micro-organismes sont utilisés dans de nombreux domaines (agro-alimentaire, pharmaceutique, environnemental, énergétique,…) que ce soit pour la production de biomasse ou de métabolites particuliers. Le passage de l’échelle du laboratoire à l’échelle industrielle est souvent problématique car les micro-organismes sont sensibles aux conditions environnementales développées au sein du volume réactionnel. De plus, la perte de l’efficacité d’homogénéisation des bioréacteurs industriels entraîne des perturbations au niveau du métabolisme des cellules. C’est pourquoi il est important d’étudier les conditions hydrodynamiques développées au sein du réacteur. Cet article présente des méthodes de calcul et de modélisation de ces conditions. L’impact de l’hydrodynamique sur le métabolisme microbien peut être étudié par l’utilisation de réacteurs scale-down. Les techniques nouvelles permettant de réaliser un scale-up prennent en compte des caractères physiologiques des cellules, qui estiment l’impact des conditions environnementales sur le métabolisme microbien et donc la bonne réussite du procédé. Enfin, l’étude de Saccharomyces cerevisiae, micro-organisme de grand intérêt biotechnologique, est réalisée. [less ▲]

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