Indirect photometric detection of amino acids in capillary zone electrophoresis

in Journal de Pharmacie de Belgique (1991), 46

Dosage entièrement automatisé de l'indométhacine dans le plasma, par extraction liquide-solide sur cartouches à usage unique associée à la chromatographie liquide haute performance

Master of advanced studies dissertation (1991)

Fully automated determination of verapamil and norverapamil in plasma using liquid-solid extraction and HPLC with fluorescence detection

Poster (1991)

Optimisation of automated liquid-solid extraction using disposable cartridges in the HPLC analysis of drugs in plasma

Poster (1990)

A Fully Automated High-Performance Liquid Chromatographic Method for the Determination of Indomethacin in Plasma

in Journal of Pharmaceutical & Biomedical Analysis (1989), 7(12), 1819-27

A fully automated method is described, which enables the determination of indomethacin in plasma by reversed-phase HPLC following on-line sample enrichment and clean-up on a short pre-column. The plasma ... [more ▼]

A fully automated method is described, which enables the determination of indomethacin in plasma by reversed-phase HPLC following on-line sample enrichment and clean-up on a short pre-column. The plasma sample is introduced directly into the column switching system. The pre-column, filled with a pellicular bonded phase, is first washed with phosphate buffer, pH 7.4. The compounds retained on the pre-column are then eluted in the fore-flush mode and separated on an octadecylsilica column with a methanolic phosphate buffer (pH 7.4) mobile phase. Indomethacin is determined spectrophotometrically at 254 or 260 nm. The effect of changes in the pH and flow rate of the washing eluent are studied. Under the conditions selected, memory effects can be avoided, the absolute recovery of the drug is 70% and the limit of detection 10 ng ml-1 for a 100 microliter injection of plasma. At a concentration of 100 ng ml-1, the relative standard deviations (RSD) are 1.7% (within-day) and 3.5% (between-day), respectively. [less ▲]