References of "Habraken, Yvette"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailBronchoalveolar lavage fluids of patients with lung injury activate the transcription factor nuclear factor-kappa beta in an alveolar cell line
Nys, Monique ULg; Deby-Dupont, G.; Habraken, Yvette ULg et al

in Clinical Science (2002), 103(6), 577-585

In bronchoalveolar lavage (BAL) fluid from ventilated patients, cytotoxic oxidant activity is correlated with neutrophil activation. The aim of the present study was to investigate the hypothesis that BAL ... [more ▼]

In bronchoalveolar lavage (BAL) fluid from ventilated patients, cytotoxic oxidant activity is correlated with neutrophil activation. The aim of the present study was to investigate the hypothesis that BAL fluid induces activation of the transcription nuclear factor-kappaB (NF-kappaB) in human alveolar cells, in correlation with inflammatory mediators. We measured endotoxin, inflammatory cytokines [Interleukin-1beta (IL-1beta), IL-8], nitrated proteins and the activity of myeloperoxidase (MPO) in BAL fluid from ventilated patients developing bronchopneumonia (n = 19 samples) or with acute respiratory distress syndrome (ARDS) (n = 14), and from ARDS/infection-free patients (n = 11). We also exposed alveolar cells to the BAL fluid or to human MPO, H2O2 or HOCl, and tested nuclear extracts for the activation of NF-kappaB. IL-1beta, IL-8, nitrated protein, MPO and endotoxin levels were significantly higher in BAL fluid from patients with bronchopneumonia than in that from the ARDS and ARDS/infection-free groups. A correlation was observed between IL-8 and MPO values (r = 0.82). The level of NF-kappaB activity induced by the BAL fluid was correlated with levels of IL-1beta (P < 0.001), IL-8 (P < 0.005) and MPO (P < 0.002), and with the neutrophil count (P < 0.002), and was higher for BAL fluid from the bronchopneumonia group. NF-kappaB activation by MPO was also demonstrated. The activation of NF-kappaB by BAL fluid, especially that from bronchopneumonia patients, suggests that a similar phenomenon may occur in vivo, leading to potential amplification of the inflammatory reaction. [less ▲]

Detailed reference viewed: 50 (8 ULg)
See detailActivation of the NF-kappaB transcription factor by Camptothecin
Habraken, Yvette ULg; Piette, Jacques ULg

Poster (2002, January 30)

We used Camptothecin (CPT) a DNA topoisomerase I inhibitor to introduce double-strand breaks (DSB) in DNA. CPT and derivatives presently used in chemotherapy are known to have maximal cytotoxicity for ... [more ▼]

We used Camptothecin (CPT) a DNA topoisomerase I inhibitor to introduce double-strand breaks (DSB) in DNA. CPT and derivatives presently used in chemotherapy are known to have maximal cytotoxicity for cells in S phase. We have shown that the activation of NF-κB and its transcriptional activity are enhanced in S phase HeLa cells. The CPT-induced activation of NF- κB is slow but stable. However, the composition of the complex evolves with time from mostly p50/p65 after 2 h to almost exclusively p52 after 24 h. The signal transduction progresses through the activation of the IKK complex, the phosphorylation of IκBα on S32 and S36 and the degradation of the inhibitor by the 26S proteasome. The transient expression of a kinase inactive mutant of NIK abolishes the activation of NF-κB by CPT indicating that this kinase could play a role upstream of the IKK complex whereas a dominant negative form of MEKK1 has no effect. To better understand the early steps of the signaling cascade initiated by the DSB, we compare the induction of NF-κB in both NBS-/- and NBS+/+ fibroblasts. NBS turn out to be implicated in NF-κB activation by X-rays, it does not seems to be important for activation by CPT supporting the idea that the components of the signaling cascade are not identical. [less ▲]

Detailed reference viewed: 19 (0 ULg)
See detailActivation of the NF-kappaB transcription factor by DNA damage
Habraken, Yvette ULg; Piette, Jacques ULg

Poster (2002, January 07)

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailS Phase Dependence and Involvement of Nf-Kappab Activating Kinase to Nf-Kappab Activation by Camptothecin
Habraken, Yvette ULg; Piret, Bernard; Piette, Jacques ULg

in Biochemical Pharmacology (2001), 62(5), 603-16

Camptothecin (CPT) and derivatives are topoisomerase I poisons currently used as anticancer drugs. Their cytotoxicity is maximal for cells in S phase. Using asynchronous and S phase-synchronized HeLa ... [more ▼]

Camptothecin (CPT) and derivatives are topoisomerase I poisons currently used as anticancer drugs. Their cytotoxicity is maximal for cells in S phase. Using asynchronous and S phase-synchronized HeLa cells, we showed that both the nuclear factor-kappaB (NF-kappaB) activation and its transcriptional activity, induced by CPT treatment, are enhanced in S phase cells. After CPT treatment, NF-kappaB activation reached a maximum within 2-3 hr and was still detectable after 24 hr. The nature of the complex evolved with time, forming mostly p50/p65 after 2 hr to almost exclusively p52 after 24 hr. In HeLa cells, the different steps of the induction were readily observable in S phase synchronized cells, whereas they were barely noticeable in a randomly growing cell population. The signal progressed through the activation of the IKK complex, the phosphorylation of IkappaBalpha, and the degradation of phosphorylated-IkappaBalpha and -IkappaBbeta. The stable expression of wild-type HA-tagged-IkappaBalpha or mutated HA-tagged-IkappaBalpha (S32,36A) allowed us to confirm the essential role of Ser32 and Ser36. NF-kappaB-activating kinase (NIK) could play a role upstream of the IKK complex, as the transient expression of a kinase inactive mutant NIK(K429,430A) abolished the activation of NF-kappaB by CPT. A kinase inactive mutant of mitogen-activated protein/ERK kinase kinase 1 (MEKK1), another kinase susceptible of acting upstream of the signalsome, did not. Cytotoxicity studies with clonal populations expressing different amounts of wild-type or mutated IkappaBalpha revealed that the overexpression of wild-type IkappaBa in large amount increases the sensitivity of HeLa cells to CPT more efficiently than a lower level of expression of non-phosphorylable IkappaBalpha. [less ▲]

Detailed reference viewed: 33 (5 ULg)
See detailActivation of the NF-kappaB transcription factor by Camptothecin in HeLa cells
Habraken, Yvette ULg; Piret, Bernard; Piette, Jacques ULg

Poster (2000, February 22)

Detailed reference viewed: 12 (1 ULg)
See detailDNA repair mechanisms: implication in cancer
Habraken, Yvette ULg

Post doctoral thesis (1999)

Detailed reference viewed: 14 (0 ULg)
Full Text
Peer Reviewed
See detailATP dependeny assembly of a ternary complex consisting of DNA Mismatch and the yeast Msh2-Msh6 and Mlh1-Pms1 protein complexes
Habraken, Yvette ULg; Sung, Patrick; Prakash, Louise et al

in Journal of Biological Chemistry (1998), 273(16), 9837-41

Detailed reference viewed: 6 (0 ULg)
Full Text
Peer Reviewed
See detailEnhancement of MSH2-MSH3-mediated mismatch repair recognition by the yeast MLH1-PMS1 complex
Habraken, Yvette ULg; Sung, Patrick; Prakash, Louise et al

in Current Biology (1997), 7(10), 790-3

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailEvidence of involvement of yeast proliferating cell nuclear antigen in DNA mismatch repair
Johnson, Robert; Kovvali, G.; Guzder, Sami et al

in Journal of Biological Chemistry (1996), 271(45), 27897-90

Detailed reference viewed: 6 (1 ULg)
Full Text
Peer Reviewed
See detailTranscription factor TFIIH and DNA endonuclease Rad2 constitute yeast nucleotide excision repair factor 3: implications for nucleotide excision repair and Cockayne syndrome
Habraken, Yvette ULg; Sung, Patrick; Prakash, Louise et al

in Proceedings of the National Academy of Sciences of the United States of America (1996), 93(20), 10718-22

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailBinding of insertion/deletion DNA mismatches by the heterodimer of yeast mismatch repair protein MSH2 and MSH3
Habraken, Yvette ULg; Sung, Patrick; Prakash, Louise et al

in Current Biology (1996), 6(9), 1185-7

Detailed reference viewed: 12 (1 ULg)
Full Text
Peer Reviewed
See detailRAD26, the yeast homolog of human Cockayne 's syndrome group B, encodes a DNA dependent ATPase
Guzder, Sami; Habraken, Yvette ULg; Sung, Patrick et al

in Journal of Biological Chemistry (1996), 271(31), 18314-7

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailStructure-specific nuclease activity in yeast nucleotide excision repair protein RAD2
Habraken, Yvette ULg; Sung, Patrick; Prakash, Louise et al

in Journal of Biological Chemistry (1995), 270(50), 30194-8

Detailed reference viewed: 17 (0 ULg)
Full Text
Peer Reviewed
See detailReconstitution of yeast nucelotide excision repair with purified RAD proteins, replication protein A and transcription factor TFIIH
Guzder, Sami; Habraken, Yvette ULg; Sung, Patrick et al

in Journal of Biological Chemistry (1995), 270(22), 12973-6

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailA conserved 5' to 3' exonuclease activity in yeast and human nucleotide excision repair protein RAD2 and XPG
Habraken, Yvette ULg; Sung, Patrick; Prakash, Louise et al

in Journal of Biological Chemistry (1994), 269(50), 31342-5

Detailed reference viewed: 8 (0 ULg)
Peer Reviewed
See detailHolliday junction cleavage by yeast Rad1 protein
Habraken, Yvette ULg; Sung, Patrick; Prakash, Louise et al

in Nature (1994), 371

Detailed reference viewed: 19 (0 ULg)
Full Text
Peer Reviewed
See detailHuman xeroderman group G gene encodes a DNA endonuclease
Habraken, Yvette ULg; Sung, Patrick; Prakash, Louise et al

in Nucleic Acids Research (1994), 22(16), 3312-6

Detailed reference viewed: 5 (2 ULg)
Peer Reviewed
See detailYeast excision repair RAD2 encodes a single-stranded DNA endonuclease
Habraken, Yvette ULg; Sung, Partick; Prakash, Louise et al

in Nature (1993), 366

Detailed reference viewed: 7 (0 ULg)