Improvement of the Detection of Neurodegenerative Alzheimer's Disease through a Specific Surface Chemistry Applied onto the Inner Surface of the Titration Well.
; ; Zorzi, Willy et al
in Journal of Biomaterials Science. Polymer Edition (2011)Detailed reference viewed: 15 (3 ULg)
Study of the Adhesion of Neurodegenerative Proteins on Plasma-Modified and Coated Polypropylene Surfaces.
; ; et al
in Journal of Biomaterials Science. Polymer Edition (2011)Detailed reference viewed: 35 (3 ULg)
Detection of Clostridium botulinum neurotoxins A and B in milk by ELISA and immuno-PCR at higher sensitivity than mouse bio-assay
; Elmoualij, Benaïssa ; et al
in Food Analytical Methods (2011), [Epub ahead of print]Detailed reference viewed: 54 (9 ULg)
Alzheimer’s diseases: towards biomarkers for an early diagnosis
Elmoualij, Benaïssa ; Dupiereux-Fettweis, Ingrid ; et al
Book published by InTech (2011)Detailed reference viewed: 36 (8 ULg)
MODULATING EFFECT OF THE RESPIRATORY RATE OF CARDIOMYOCYTES (H9C2 CELLS) AND MITOCHONDRIAL SWELLING BY DIAZOXIDE ANALOGUES: STRUCTURE–FUNCTION RELATIONSHIPS
Ceusters, Justine ; Mouithys-Mickalad, Ange ; De Tullio, Pascal et al
Poster (2011)Detailed reference viewed: 24 (5 ULg)
Adenosine thiamine triphosphate accumulates in Escherichia coli cells in response to specific conditions of metabolic stress
Gigliobianco, Tiziana ; Lakaye, Bernard ; et al
in BMC Microbiology (2010), 10Detailed reference viewed: 35 (19 ULg)
Thiamine triphosphate synthesis in rat brain occurs in mitochondria and is coupled to the respiratory chain
Gangolf, Marjorie ; ; Thiry, Marc et al
in Journal of Biological Chemistry (2010), 285Detailed reference viewed: 83 (34 ULg)
Ku proteins interact with activator protein-2 transcription factors and contribute to ERBB2 overexpression in breast cancer cell lines.
Nolens, Grégory ; Pignon, Jean-Christophe ; Koopmansch, Benjamin et al
in Breast Cancer Research [=BCR] (2009), 11(6),
INTRODUCTION: Activator protein-2 (AP-2) alpha and AP-2 gamma transcription factors contribute to ERBB2 gene overexpression in breast cancer. In order to understand the mechanism by which the ERBB2 gene ... [more ▼]
INTRODUCTION: Activator protein-2 (AP-2) alpha and AP-2 gamma transcription factors contribute to ERBB2 gene overexpression in breast cancer. In order to understand the mechanism by which the ERBB2 gene is overexpressed we searched for novel AP-2 interacting factors that contribute to its activity. METHODS: Ku proteins were identified as AP-2 alpha interacting proteins by glutathione serine transferase (GST)-pull down followed by mass spectrometry. Transfection of the cells with siRNA, expression vectors and reporter vectors as well as chromatin immunoprecipitation (ChIP) assay were used to ascertain the implication of Ku proteins on ERBB2 expression. RESULTS: Nuclear proteins from BT-474 cells overexpressing AP-2 alpha and AP-2 gamma were incubated with GST-AP2 or GST coated beads. Among the proteins retained specifically on GST-AP2 coated beads Ku70 and Ku80 proteins were identified by mass spectrometry. The contribution of Ku proteins to ERBB2 gene expression in BT-474 and SKBR3 cell lines was investigated by downregulating Ku proteins through the use of specific siRNAs. Depletion of Ku proteins led to downregulation of ERBB2 mRNA and protein levels. Furthermore, reduction of Ku80 in HCT116 cell line decreased the AP-2 alpha activity on a reporter vector containing an AP-2 binding site linked to the ERBB2 core promoter, and transfection of Ku80 increased the activity of AP-2 alpha on this promoter. Ku siRNAs also inhibited the activity of this reporter vector in BT-474 and SKBR3 cell lines and the activity of the ERBB2 promoter was further reduced by combining Ku siRNAs with AP-2 alpha and AP-2 gamma siRNAs. ChIP experiments with chromatin extracted from wild type or AP-2 alpha and AP-2 gamma or Ku70 siRNA transfected BT-474 cells demonstrated Ku70 recruitment to the ERBB2 proximal promoter in association with AP-2 alpha and AP-2 gamma. Moreover, Ku70 siRNA like AP-2 siRNAs, greatly reduced PolII recruitment to the ERBB2 proximal promoter. CONCLUSIONS: Ku proteins in interaction with AP-2 (alpha and gamma) contribute to increased ERBB2 mRNA and protein levels in breast cancer cells. [less ▲]Detailed reference viewed: 56 (17 ULg)
La décontamination des prions selon les méthodes plasmas
Elmoualij, Benaïssa ; Thellin, Olivier ; Zorzi, Willy
in Bioadh 2009 (2009, November 03)Detailed reference viewed: 4 (4 ULg)
Prion: Biosafety Level 3-unconventional transmissible agent
Zorzi, Willy ; Elmoualij, Benaïssa
Conference (2009, April 22)Detailed reference viewed: 17 (1 ULg)
A decade of improvements in quantification of gene expression and internal standard selection.
Thellin, Olivier ; Elmoualij, Benaïssa ; Heinen, Ernst et al
in Biotechnology Advances (2009)
Major improvements have been made in mRNA quantification and internal standard selection over the last decade. Our aim in this paper is to present the main developments that are of interest for practical ... [more ▼]
Major improvements have been made in mRNA quantification and internal standard selection over the last decade. Our aim in this paper is to present the main developments that are of interest for practical laboratory work, contrasting the situation as it is now with the one of ten years ago, and presenting some excellent examples of what can be done today. Specifically, we will mainly discuss Real-Time RT-PCR major improvements that have been performed in the following areas: the most commonly used quantification techniques, the mathematical and software tools created to help researchers in their work on internal standard selection, the availability of detection chemistries and technical information and of commercial tools and services. In addition to mRNA quantification, we will also discuss some aspects of non-coding RNA and protein quantification. In addition to technical improvements, the development of international cooperation and the creation of technical databases are likely to represent a major tool for the future in the standardization of gene expression quantification. [less ▲]Detailed reference viewed: 41 (3 ULg)
Creutzfeldt-jakob, Parkinson, lewy body dementia and Alzheimer diseases: from diagnosis to therapy.
Dupiereux-Fettweis, Ingrid ; Zorzi, Willy ; et al
in Central Nervous System Agents in Medicinal Chemistry (2009), 9(1), 2-11
Depositions of proteins in form of amyloid and non-amyloid plaques are common pathogenic signs of more than 20 degenerative diseases affecting the central nervous system or a variety of peripheral tissues ... [more ▼]
Depositions of proteins in form of amyloid and non-amyloid plaques are common pathogenic signs of more than 20 degenerative diseases affecting the central nervous system or a variety of peripheral tissues. Among the neuropathological conditions, Alzheimer's, Parkinson's and the prion diseases, such as Creutzfeldt-Jakob disease (CJD), present ambiguities as regarding their differential diagnosis. At present, their diagnosis must be confirmed by post-mortem examination of the brain. Currently the ante-mortem diagnosis is still based on the integration of multiple data (clinical, paraclinical and biological analyses) because no unique marker exists for such diseases. The detection of specific biomarkers would be useful to develop a differential diagnostic, distinguishing not only different neurodegenerative diseases but also the disease from the non-pathological effects of aging. Several neurodegenerative biomarkers are present at very low levels during the early stages of the disease development and their ultra-low detection is needed for early diagnosis, which should permit more effective therapeutic interventions, before the disease concerned can progress to a stage where considerable damage to the brain has already occurred. In the case of prion diseases, there are concerns regarding not only patient care, but the wider community too, with regard to the risk of transmission of prions, especially during blood transfusion, for which, four cases of variant CJD infection associated with transfusion of non-leukocyte-depleted blood components have been confirmed. Therefore the development of techniques with high sensitivity and specificity represent the major challenge in the field of the protein misfolding diseases. In this paper we review the current analytical and/or biochemical diagnostic technologies used mainly in prion, but also in Alzheimer and Parkinson diseases and emphasizing work on the protein detection as a surrogates and specific biomarker in the body fluid of patients (urine, CSF and blood). This review highlights the urgency of the development of early and sensitive diagnostics in terms of therapeutic challenge. [less ▲]Detailed reference viewed: 99 (5 ULg)
Immuno PCR : a new tool for the ultra-sensitive detection of biomolecules
Zorzi, Willy ; Elmoualij, Benaïssa
Conference (2008, December 11)Detailed reference viewed: 5 (0 ULg)
A proteomic study to investigate the role of the prion protein in the cellular defence against oxidative stress.
Dupiereux-Fettweis, Ingrid ; ; Zorzi, Willy et al
Poster (2008)Detailed reference viewed: 7 (0 ULg)