Release of cardiac biomarkers after an intense physical exercise: preliminary results

Poster (2010, March 20)

Background: Over the past 2 decades, there has been a large interest in cardiac markers elevations, which are often seen following endurance sport events. These elevations were transient, with levels ... [more ▼]

Background: Over the past 2 decades, there has been a large interest in cardiac markers elevations, which are often seen following endurance sport events. These elevations were transient, with levels decreasing to pre-event concentrations within 24-48 hours. This might be explained by the relatively short half-life of studied markers, or water imbalance during and after the event. Therefore, the present preliminary study aimed to examine the increase in N-terminal pro-brain natriuretic peptide (NT-proBNP), highly sensitivity cardiac troponin T (hsTnT) and I (TnI II), myoglobin, creatine kinase muscle – brain (CK-MB), myeloperoxydase (MPO) and Highly sensitive C-reactive protein (hs-CRP) elevations after prolonged strenuous exercise . Materials and methods: Blood samples (EDTA plasma and heparinised plasma) were drawn at baseline, after 45, 90, 105, 165, 225, 285, 345, 690 and 1440 minutes in two healthy persons (29 year, trained 6 hours per week; 23 year, untrained). Each subjects runs at the maximal possibility during 2 hours. Results: For the untrained person, level of NT-proBNP exceeded the upper reference limits 12 hours after exercise but increased in all times. HsTnT and TnI II levels were upper the reference limit respectively 45 minutes and directly after exercise and increased up to 4 hours after exercise. We reported a decrease of these concentrations above the reference limits after 24 hours. Myoglobin increased after 45 minutes until 5 hours after exercises. It decreased after the 5th hour to be normalized 24 hours after exercise. CK-MB increased directly after the exercise and was upper the reference limits 165 minutes after the exercise. Level of MPO was very high just after exercise and decreased quickly in the following hours to be just upper the limit references 24 hours after exercise. HsCRP levels increased after 105 minutes and continued to increase after 24 hours. For the trained subject, we noted the same profile of increase of cardiac markers levels stayed but in the range of reference. Conclusion: These cases are extremely interesting. Indeed, this observation suggested a physiological counter regulatory process rather a simple increase of myocardial damage related to the intensity of exercise. In fact, for this moment, we do not know if the release of cardiac markers is physiological or pathological thus it must be studied. This preliminary study on endurance training suggested that intensively is determinants of the rate and the magnitude of subsequent cardiac marker release. These results suggested that an adaptation mechanism could exist. Benefits and possible long-term negative aspects of prolonged exercise should be evaluated with a more important population of athletes. [less ▲]

Comparaison des taux de BNP et de NT-proBNP chez des patients insuffisants rénaux pour prévenir l'insuffisance cardiaque

in Biotribune Magazine (2010), 34

Background: The aim of the study is to compare the performances of BNP and NT-proBNP for diagnosing heart failure (HF) in a population of patients with high incidence of chronic renal insufficiency (CRI ... [more ▼]

Background: The aim of the study is to compare the performances of BNP and NT-proBNP for diagnosing heart failure (HF) in a population of patients with high incidence of chronic renal insufficiency (CRI, plasma creatinine > 1.5 mg/dl). Patients and methods: Ninety-eight patients were included in this study. BNP and NT-proBNP determinations were performed by an immunofluorescent assay (Biosite®) and by an electrochemiluminescence sandwich immuno assay (Roche Diagnostic®), respectively. Results and discussion: BNP and NTproBNP level are correlated in CRI and non CRI . Both assays are useful to rule out CRI pts suspected of HF. However, in renal failure pts, higher decision limits should be used for improving the positive predictive value of the assays. [less ▲]

BCL-3 degradation involves its polyubiquitination through a FBW7-independent pathway and its binding to the proteasome subunit PSMB1.

in Journal of Biological Chemistry (2010), 285(33), 2583125840

The oncogenic protein BCL-3 activates or represses gene transcription through binding with the NF-kappaB proteins p50 and p52 and is degraded through a phospho- and GSK3-dependent pathway. However, the ... [more ▼]

The oncogenic protein BCL-3 activates or represses gene transcription through binding with the NF-kappaB proteins p50 and p52 and is degraded through a phospho- and GSK3-dependent pathway. However, the mechanisms underlying its degradation remain poorly understood. Yeast-two-hybrid analysis led to the identification of the proteasome subunit PSMB1 as a BCL-3-associated protein. The binding of BCL-3 to PSMB1 is required for its degradation through the proteasome. Indeed, PSMB1-depleted cells are defective in degrading polyubiquitinated BCL-3. The N-terminal part of BCL-3 includes lysines 13 and 26 required for the K48-linked polyubiquitination of BCL-3. Moreover, the E3 ligase FBW7 known to polyubiquitinate a variety of substrates phosphorylated by GSK3 is dispensable for BCL-3 degradation. Thus, our data defined an unique motif of BCL-3 that is needed for its recruitment to the proteasome and identified PSMB1 as a key protein required for the proteasome-mediated degradation of a nuclear and oncogenic IkappaB protein. [less ▲]

Measurement uncertainty of 25-OH vitamin D determination with different commercially available kits: impact on the clinical cut offs

in Osteoporosis International (2010), 21

Component-resolved-diagnosis and validation of an allergen-microarray technology.

in Allergy (2010), 65(suppl. 92), 93

Corrélation aldostérone et parathormone en cas d'hyperparathyroïdie primaire vs secondaire?

in Annales de Biologie Clinique (2010), 68(6), 703-4

Evaluation biologique du stress oxydant : application en routine clinique.

in Nutritions & Endocrinologie (2009), HS Stress Oxydant

CRD and technical validation of ImmunoCAPISAC

Conference (2009, November 19)

Myéloperoxydase, LDL oxydées et anticorps anti-LDL oxydées sont-ils interdépendants ?

Poster (2009, November 03)

Measrement of HBA1c using the new vitros 5600 integrated system.

Poster (2009, November)