The emerging role of lysine acetylation of non-nuclear proteins
Close, Pierre ; ; Gillard, Magali et al
in Cellular and Molecular Life Sciences : CMLS (2010), 67(8), 1255-1264
Lysine acetylation is a post-translational modification that critically regulates gene transcription by targeting histones as well as a variety of transcription factors in the nucleus. More recent reports ... [more ▼]
Lysine acetylation is a post-translational modification that critically regulates gene transcription by targeting histones as well as a variety of transcription factors in the nucleus. More recent reports have also demonstrated that numerous proteins located outside the nucleus are also acetylated and that this modification has profound consequences on their functions. This review describes the latest findings on the substrates acetylated outside the nucleus and on the acetylases and deacetylates that catalyse these modifications. Protein acetylation is emerging as a major mechanism by which key proteins are regulated in many physiological processes such as migration, metabolism and aging as well as in pathological circumstances such as cancer and neurodegenerative disorders. [less ▲]Detailed reference viewed: 120 (22 ULg)
Release of cardiac biomarkers after an intense physical exercise: preliminary results
Le Goff, Caroline ; ; Chapelle, Jean-Paul et al
Poster (2010, March 20)
Background: Over the past 2 decades, there has been a large interest in cardiac markers elevations, which are often seen following endurance sport events. These elevations were transient, with levels ... [more ▼]
Background: Over the past 2 decades, there has been a large interest in cardiac markers elevations, which are often seen following endurance sport events. These elevations were transient, with levels decreasing to pre-event concentrations within 24-48 hours. This might be explained by the relatively short half-life of studied markers, or water imbalance during and after the event. Therefore, the present preliminary study aimed to examine the increase in N-terminal pro-brain natriuretic peptide (NT-proBNP), highly sensitivity cardiac troponin T (hsTnT) and I (TnI II), myoglobin, creatine kinase muscle – brain (CK-MB), myeloperoxydase (MPO) and Highly sensitive C-reactive protein (hs-CRP) elevations after prolonged strenuous exercise . Materials and methods: Blood samples (EDTA plasma and heparinised plasma) were drawn at baseline, after 45, 90, 105, 165, 225, 285, 345, 690 and 1440 minutes in two healthy persons (29 year, trained 6 hours per week; 23 year, untrained). Each subjects runs at the maximal possibility during 2 hours. Results: For the untrained person, level of NT-proBNP exceeded the upper reference limits 12 hours after exercise but increased in all times. HsTnT and TnI II levels were upper the reference limit respectively 45 minutes and directly after exercise and increased up to 4 hours after exercise. We reported a decrease of these concentrations above the reference limits after 24 hours. Myoglobin increased after 45 minutes until 5 hours after exercises. It decreased after the 5th hour to be normalized 24 hours after exercise. CK-MB increased directly after the exercise and was upper the reference limits 165 minutes after the exercise. Level of MPO was very high just after exercise and decreased quickly in the following hours to be just upper the limit references 24 hours after exercise. HsCRP levels increased after 105 minutes and continued to increase after 24 hours. For the trained subject, we noted the same profile of increase of cardiac markers levels stayed but in the range of reference. Conclusion: These cases are extremely interesting. Indeed, this observation suggested a physiological counter regulatory process rather a simple increase of myocardial damage related to the intensity of exercise. In fact, for this moment, we do not know if the release of cardiac markers is physiological or pathological thus it must be studied. This preliminary study on endurance training suggested that intensively is determinants of the rate and the magnitude of subsequent cardiac marker release. These results suggested that an adaptation mechanism could exist. Benefits and possible long-term negative aspects of prolonged exercise should be evaluated with a more important population of athletes. [less ▲]Detailed reference viewed: 41 (8 ULg)
Anticorps anti-gliadines déamidées et maladie coeliaque : données actuelles et évaluation des faux positifs de cinq trousses de dosage
Lutteri, Laurence ; ; Chapelle, Jean-Paul
in Annales de Biologie Clinique (2010), 68(2), 149-56
Recently, anti-deamidated gliadin antihodies were proposed for the serological diagnosis of celiac disease. We evaluate the specificity of different anti-deamidated gliadin antibodies ELISA in comparison ... [more ▼]
Recently, anti-deamidated gliadin antihodies were proposed for the serological diagnosis of celiac disease. We evaluate the specificity of different anti-deamidated gliadin antibodies ELISA in comparison with conventional anti-native gliadin kits. Serum sainples froin 46 non celiac patients trere analyzed by five different quantitative ELISA for anti-native gliadin, antideamidated gliadin and anti-fransglutaininase neo-epitope antibodies together with a screening ELISA. Twenty-four percent of the patients deinonstrated anti-native gliadin lgA and 63% 1gO antibodies. Using anti-dearnidated gliadin antibodies, tire number of false positive IgA and, particularly, 1gG results,markedly decreased in the non celiac patients: 21 and 24% respectively with anti-Gliadin (GAF-3X) Euroimmun kit, 7 and 26% with Bindazyme 1-lurnan Anti-Gliadin (MGP) The Binding Site kit and 0 and 41% with Celiac G+ Immco kit. The new assay which makes use of the physiological complex of tissue transglutaminase cross-linked with deamidated gliadin peptides, called neo-epitope, did not improve the differential diagnosis of celiac disease with 30% of false positive results in IgG (2% in IgA). IJsing the Inova screening kit, a positive resuit for IgA and/or IgG anti-deamidated gliadin and!or anti- tissue transglutaminase antibodies was obtained in 24% of the non celiac patients. In conclusion, our study assessed the superiority, in ternis of specificity, of anti-deamidated gliadin antibodies, over the conventional anti-gliadin antibodies for die differential diagnosis of celiac disease. [less ▲]Detailed reference viewed: 255 (4 ULg)
Comparaison des taux de BNP et de NT-proBNP chez des patients insuffisants rénaux pour prévenir l'insuffisance cardiaque
Le Goff, Caroline ; Kaux, Jean-François ; Bovy, Christophe et al
in Biotribune Magazine (2010), 34
Background: The aim of the study is to compare the performances of BNP and NT-proBNP for diagnosing heart failure (HF) in a population of patients with high incidence of chronic renal insufficiency (CRI ... [more ▼]
Background: The aim of the study is to compare the performances of BNP and NT-proBNP for diagnosing heart failure (HF) in a population of patients with high incidence of chronic renal insufficiency (CRI, plasma creatinine > 1.5 mg/dl). Patients and methods: Ninety-eight patients were included in this study. BNP and NT-proBNP determinations were performed by an immunofluorescent assay (Biosite®) and by an electrochemiluminescence sandwich immuno assay (Roche Diagnostic®), respectively. Results and discussion: BNP and NTproBNP level are correlated in CRI and non CRI . Both assays are useful to rule out CRI pts suspected of HF. However, in renal failure pts, higher decision limits should be used for improving the positive predictive value of the assays. [less ▲]Detailed reference viewed: 193 (7 ULg)
Analytical validation of the BAP OSTASE on Liaison (DiaSorin).
Cavalier, Etienne ; Rozet, Eric ; Carlisi, Ignazia et al
in Clinical Chemistry & Laboratory Medicine (2010), 48(1), 67-72Detailed reference viewed: 105 (13 ULg)
BCL-3 degradation involves its polyubiquitination through a FBW7-independent pathway and its binding to the proteasome subunit PSMB1.
Keutgens, Aurore ; Zhang-Shao, Xin ; Shostak, Kateryna et al
in Journal of Biological Chemistry (2010), 285(33), 2583125840
The oncogenic protein BCL-3 activates or represses gene transcription through binding with the NF-kappaB proteins p50 and p52 and is degraded through a phospho- and GSK3-dependent pathway. However, the ... [more ▼]
The oncogenic protein BCL-3 activates or represses gene transcription through binding with the NF-kappaB proteins p50 and p52 and is degraded through a phospho- and GSK3-dependent pathway. However, the mechanisms underlying its degradation remain poorly understood. Yeast-two-hybrid analysis led to the identification of the proteasome subunit PSMB1 as a BCL-3-associated protein. The binding of BCL-3 to PSMB1 is required for its degradation through the proteasome. Indeed, PSMB1-depleted cells are defective in degrading polyubiquitinated BCL-3. The N-terminal part of BCL-3 includes lysines 13 and 26 required for the K48-linked polyubiquitination of BCL-3. Moreover, the E3 ligase FBW7 known to polyubiquitinate a variety of substrates phosphorylated by GSK3 is dispensable for BCL-3 degradation. Thus, our data defined an unique motif of BCL-3 that is needed for its recruitment to the proteasome and identified PSMB1 as a key protein required for the proteasome-mediated degradation of a nuclear and oncogenic IkappaB protein. [less ▲]Detailed reference viewed: 75 (35 ULg)
Comparison of three methods for fractionation and enrichment of low molecular weight proteins for SELDI-TOF-MS differential analysis
De Bock, Muriel ; De Seny, Dominique ; Meuwis, Marie-Alice et al
in Talanta (2010), 82
In most diseases, the clinical need for serum/plasma markers has never been so crucial, not only for diagnosis, but also for the selection of the most efficient therapies, as well as exclusion of ... [more ▼]
In most diseases, the clinical need for serum/plasma markers has never been so crucial, not only for diagnosis, but also for the selection of the most efficient therapies, as well as exclusion of ineffective or toxic treatment. Due to the high sample complexity, prefractionation is essential for exploring the deep proteome and finding specific markers. In this study, three different sample preparation methods (i.e., highly abundant protein precipitation, restricted access materials (RAM) combined with IMAC chromatography and peptide ligand affinity beads) were investigated in order to select the best fractionation step for further differential proteomic experiments focusing on the LMW proteome (MW inferior to 40,000 Da). Indeed, the aim was not to cover the entire plasma/serum proteome, but to enrich potentially interesting tissue leakage proteins. These three methods were evaluated on their reproducibility, on the SELDI-TOF-MS peptide/protein peaks generated after fractionation and on the information supplied. The studied methods appeared to give complementary information and presented good reproducibility (below 20%). Peptide ligand affinity beads were found to provide efficient depletion of HMW proteins and peak enrichment in protein/peptide profiles. [less ▲]Detailed reference viewed: 62 (16 ULg)
Measurement uncertainty of 25-OH vitamin D determination with different commercially available kits: impact on the clinical cut offs
Cavalier, Etienne ; Rozet, Eric ; Gadisseur, Romy et al
in Osteoporosis International (2010), 21Detailed reference viewed: 74 (10 ULg)
Multicentre evaluation of the Tosoh HbA1c G8 analyser
Chapelle, Jean-Paul ; Teixeira, Jelda ; et al
in Clinical Chemistry & Laboratory Medicine (2010), 48(3), 365-371Detailed reference viewed: 44 (3 ULg)
Component-resolved-diagnosis and validation of an allergen-microarray technology.
Gadisseur, Romy ; Chapelle, Jean-Paul ; Cavalier, Etienne
in Allergy (2010), 65(suppl. 92), 93Detailed reference viewed: 11 (1 ULg)
Component-resolved diagnosis in peanut and hazelnut allergy.
Gadisseur, Romy ; Chapelle, Jean-Paul ; Cavalier, Etienne et al
in Allergy (2010), 65(Suppl.92), 106Detailed reference viewed: 33 (1 ULg)
Corrélation aldostérone et parathormone en cas d'hyperparathyroïdie primaire vs secondaire?
Ho, Thi Thanh Giang ; Keutgens, Aurore ; Chapelle, Jean-Paul et al
in Annales de Biologie Clinique (2010), 68(6), 703-4Detailed reference viewed: 24 (6 ULg)
The ratio of PTH as measured by third and second generation assays as a marker for parathyroid carcinoma
Cavalier, Etienne ; Daly, Adrian ; Chapelle, Jean-Paul et al
in 12th European Congress of Endocrinology - ICE 2010 (2010)Detailed reference viewed: 7 (1 ULg)
Evaluation biologique du stress oxydant : application en routine clinique.
Pincemail, Joël ; Le Goff, Caroline ; Charlier, Carole et al
in Nutritions & Endocrinologie (2009), HS Stress OxydantDetailed reference viewed: 27 (6 ULg)
Estimation of the Stability of Parathyroid Hormone when Stored at -80°C for a Long Period
Cavalier, Etienne ; Delanaye, Pierre ; Hubert, Philippe et al
in Clinical Journal of the American Society of Nephrology (2009), 4(12), 1988-92Detailed reference viewed: 47 (22 ULg)
Myéloperoxydase, LDL oxydées et anticorps anti-LDL oxydées sont-ils interdépendants ?
Le Goff, Caroline ; Kaux, Jean-François ; Denooz, André et al
Poster (2009, November 03)Detailed reference viewed: 121 (7 ULg)
Cholestérol total, HDL, LDL et LDL oxydées : implications cardiovasculaires
Le Goff, Caroline ; Kaux, Jean-François ; Denooz, André et al
Poster (2009, November 03)Detailed reference viewed: 140 (7 ULg)
Measrement of HBA1c using the new vitros 5600 integrated system.
Teixeira, Jelda ; Denooz, André ; Chapelle, Jean-Paul
Poster (2009, November)Detailed reference viewed: 55 (14 ULg)