Showing results 521 to 540 of 548
 The protection of creatine kinase MM sub-bands by EDTA during storage.Chapelle, Jean-Paul  ; ; in Clinica Chimica Acta (1981), 115(3), 255-62 We examined the effect of a 5 mmol/l concentration of EDTA on the stabilization of the five serum creatine kinase MM isoenzymes, resolved by thin-layer isoelectric focusing. In patient sera, total CK and ... [more ▼] We examined the effect of a 5 mmol/l concentration of EDTA on the stabilization of the five serum creatine kinase MM isoenzymes, resolved by thin-layer isoelectric focusing. In patient sera, total CK and CK-MB activities were stable during storage of the samples for two months at 4 degrees C even in the absence of EDTA. However, EDTA stabilized the labile MM and MM1 sub-bands, which are the first to appear in the blood after the release from the damaged tissue and its addition to blood samples intended for determining the MM sub-band pattern is recommended. The stabilizing effect of EDTA was emphasized at higher temperatures. EDTA protected the CK-MM pattern in myocardium extracts made in normal serum and incubated at 37 degrees C during 40 h, but was unnecessary when myocardium was homogenized in heat-inactivated serum. It is thought that EDTA could act by inhibiting a heat-labile component of human serum. [less ▲] Detailed reference viewed: 1 (0 ULg)Early assessment of risk in patients with acute myocardial infarction.Chapelle, Jean-Paul ; ; et alin European Heart Journal (1981), 2(3), 187-96 Detailed reference viewed: 9 (0 ULg)Plasma alpha-1-acid glycoprotein and haptoglobin changes in acute myocardial infarction: relationship to early mortalityChapelle, Jean-Paul ; ; et alPoster (1980, November) Detailed reference viewed: 7 (0 ULg)Significance of plasma myoglobin levels in the assessment of acute myocardial infarctionChapelle, Jean-Paul Conference (1980, September 29) Detailed reference viewed: 2 (0 ULg)Intérêt du dosage de la myoglobine dans la stratification précoce du risque chez les patients atteints d'infarctus du myocarde aigu; Albert, Adelin ; Chapelle, Jean-Paul Poster (1980, September) Detailed reference viewed: 15 (0 ULg)Early Risk Scale in Acute Myocardial InfarctionAlbert, Adelin ; Chapelle, Jean-Paul ; Kulbertus, Henri Poster (1980, June) Detailed reference viewed: 5 (0 ULg)Infarct size estimates from myocardial thallium and phosphate scans. prognostic implications; ; et al Poster (1980, March) Detailed reference viewed: 3 (0 ULg) Further heterogeneity demonstarted for serum creatine kinase isoenzyme MMChapelle, Jean-Paul ; in Clinical Chemistry (1980), 26(3), 457-62 Serum creatine kinase (EC 2.1.3.2) isoenzyme MM was resolved by isoelectric focusing into a five-band pattern, a pattern that gradually changed after the onset of myocardial infarction. Similar changes ... [more ▼] Serum creatine kinase (EC 2.1.3.2) isoenzyme MM was resolved by isoelectric focusing into a five-band pattern, a pattern that gradually changed after the onset of myocardial infarction. Similar changes were also demonstrated in patients undergoing coronary-bypass surgery. The evolution of two CK-MB sub-bands was studied in both cases. We found that three electrophoretic bands (CK-MM, pI 7.10; MM1, pI 6.88; MB1, pI 5.61) were predominant in patterns for sera collected during the early phase of myocardial infarction, but rapidly disappeared during the following hours, whereas bands of increased electrophoretic mobility (MM2, pI 6.70; MM3, pI 6.45; MM4, pI 6.25; MB2, pI 5.34) gradually increased. MM3 was always the major band at the end of the observation period in acute myocardial infarction (mean, 61.4% of total creatine kinase activity 36 h after the peak value for total creatine kinase in serum). The CK-MM bands were also present in the serum of patients without heart disease. Changes in the electrophoretic pattern were induced by a thermolabile factor in normal human serum, which transformed the muscular or myocardial MM and MM1 bands after their release into the blood stream [less ▲] Detailed reference viewed: 9 (1 ULg)High predictive value of LDH in acute myocardial infarctionChapelle, Jean-Paul Conference (1980, February 04) Detailed reference viewed: 3 (0 ULg)Predictive value of serum enzyme determinations in acute myocardial infarction.Chapelle, Jean-Paul ; Albert, Adelin ; et alin Clinica Chimica Acta (1980), 106(1), 29-38 Detailed reference viewed: 8 (1 ULg)Predictive value of serum enzyme determinations in acute myocardial infarction.Chapelle, Jean-Paul ; ; et alin Clinica Chimica Acta (1980), 106(1), 29-38 Detailed reference viewed: 2 (0 ULg)Monitoring enzymatique par courbes étalonnées de percentiles chez l'opéré cardiaque; Chapelle, Jean-Paul ; Albert, Adelin et alPoster (1979, October 06) Detailed reference viewed: 6 (1 ULg)Strategie nouvelle en matiere d'interpretation des donnees en biologie medicale.; Albert, Adelin ; Plomteux, Guy et alin Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1979), 134(11), 659-77 Detailed reference viewed: 12 (1 ULg)Strategie nouvelle en matiere d'interpretation des donnees en biologie medicale.; ; Plomteux, Guy et alin Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1979), 134(11), 659-77 Detailed reference viewed: 11 (0 ULg)Applications de la focalisation isoélectrique en chimie cliniqueChapelle, Jean-Paul Conference (1978, December 14) Detailed reference viewed: 14 (0 ULg)Signification diagnostique et pronostique des modifications biochimiques enregistrées après chirurgie cardiaque coronarienneChapelle, Jean-Paul ; Albert, Adelin ; et alPoster (1978, October) Detailed reference viewed: 5 (0 ULg)Biochemical approach to the Prognosis of acute myocardial infarctionChapelle, Jean-Paul ; Albert, Adelin ; et alPoster (1978, June 25) Detailed reference viewed: 6 (0 ULg)Infarctus du myocarde. Diagnostic précoce, évaluation quantitative des lésions.Chapelle, Jean-Paul Conference (1978, April 27) Detailed reference viewed: 2 (0 ULg)Intérêt des paramètres biochimiques dans le pronostic à court terme de l'infarctus du myocardeChapelle, Jean-Paul ; ; Conference (1978, March 23) Detailed reference viewed: 9 (0 ULg)Interférence de l'adenylate kinase dans le dosage de la CK-MB dans la phase précoce de l'infarctus du myocardeChapelle, Jean-Paul ; ; Conference (1978, March 23) Detailed reference viewed: 10 (0 ULg) |
||
