References of "Caers, Jo"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailRapamycin prevents experimental sclerodermatous chronic graft-versus-host disease in mice
Belle, Ludovic ULg; Binsfeld, Marilène ULg; DUBOIS, Sophie ULg et al

Conference (2012)

Background: The most widely used mice model of chronic graft-versus-host disease (cGvHD) is an MHC-matched bone marrow transplantation model of sclerodermatous cGvHD. A limitation of that model is that ... [more ▼]

Background: The most widely used mice model of chronic graft-versus-host disease (cGvHD) is an MHC-matched bone marrow transplantation model of sclerodermatous cGvHD. A limitation of that model is that mortality is relatively low, making difficult to study the impact of potentially therapeutic compounds. Aims: To develop a more severe model of cGVHD and to assess the impact of Rapamycin administration in that model. Results: Lethally irradiated Balb/C mice were injected with 10x106 bone marrow cells and 70x106 splenocytes from B10.D2 donor mice. Twenty-one days later, all mice developed cGvHD. For the severe model, donor B10.D2 mice were injected with 0.5x106 splenocytes from Balb/C twenty-one days before transplantation. All mice from the severe model (n=8) died a median of 32 days while 3 of 7 mice in the classical model survived beyond day 52. Mean survival was decreased in the severe model compared to the classical model (32 days versus 37 days; p=0.0185). Recipient mice in the severe group experienced higher weight loss, hair loss and skin fi brosis. Numbers of T lymphocytes (231.9 ± 151.4 versus 951 ± 532.8; p=0.0032) and CD4+ T cells (63.25 ± 41.93 versus 135.0 ± 14.39; p=0.0018) per microliter of blood at day 21 were lower in the severe group than in the classical model. Moreover, number of regulatory T cells (Tregs) was decreased in the severe model (1.250 ± 0.8864 versus 8.000 ± 6.753; p=0.0151). We then investigated whether rapamycin administration could prevent GVHD in the severe model. All (n=8) mice treated with PBS (placebo) died a median of 32 days after transplantation, while 6 of 8 mice given 1 mg/kg/day i.p. rapamycin survived beyond day 52 (p=0.0012). Number of Tregs/μl was higher at day 21 in rapamycin-treated mice than in mice given PBS (2.000±1.195 versus 1.250±0.8864; p=0.0796). Moreover, number of naïve CD4+T (10.00±4.192 versus 30.25±5.185; p= 0.0089) and effector memory T cells (EMT) (30.67±3.180 versus 67.33±7.881; p= 0.0125) were higher in rapamycin mice. Finally, proliferation of EMT (assessed by fl ow cytometry using Ki-67) was higher in PBS than in rapamycin mice (45.28%±4.084 versus 31.90%± 2.003; p=0.0474). Conclusion: We have developed a mice model of severe cGVHD. Interestingly, rapamycin prevented death from cGVHD in that model, perhaps through in vivo expansion of Treg. [less ▲]

Detailed reference viewed: 44 (15 ULg)
Full Text
Peer Reviewed
See detailA novel mouse model for multiple myeloma (MOPC315.BM) that allows noninvasive spatiotemporal detection of osteolytic disease.
Hofgaard, PO; Jodal, HC; Bommert, K et al

in PLoS ONE (2012), 7(12), 51892

Multiple myeloma (MM) is a lethal human cancer characterized by a clonal expansion of malignant plasma cells in bone marrow. Mouse models of human MM are technically challenging and do not always ... [more ▼]

Multiple myeloma (MM) is a lethal human cancer characterized by a clonal expansion of malignant plasma cells in bone marrow. Mouse models of human MM are technically challenging and do not always recapitulate human disease. Therefore, new mouse models for MM are needed. Mineral-oil induced plasmacytomas (MOPC) develop in the peritoneal cavity of oil-injected BALB/c mice. However, MOPC typically grow extramedullary and are considered poor models of human MM. Here we describe an in vivo-selected MOPC315 variant, called MOPC315.BM, which can be maintained in vitro. When injected i.v. into BALB/c mice, MOPC315.BM cells exhibit tropism for bone marrow. As few as 10(4) MOPC315.BM cells injected i.v. induced paraplegia, a sign of spinal cord compression, in all mice within 3-4 weeks. MOPC315.BM cells were stably transfected with either firefly luciferase (MOPC315.BM.Luc) or DsRed (MOPC315.BM.DsRed) for studies using noninvasive imaging. MOPC315.BM.Luc cells were detected in the tibiofemoral region already 1 hour after i.v. injection. Bone foci developed progressively, and as of day 5, MM cells were detected in multiple sites in the axial skeleton. Additionally, the spleen (a hematopoietic organ in the mouse) was invariably affected. Luminescent signals correlated with serum myeloma protein concentration, allowing for easy tracking of tumor load with noninvasive imaging. Affected mice developed osteolytic lesions. The MOPC315.BM model employs a common strain of immunocompetent mice (BALB/c) and replicates many characteristics of human MM. The model should be suitable for studies of bone marrow tropism, development of osteolytic lesions, drug testing, and immunotherapy in MM. [less ▲]

Detailed reference viewed: 31 (1 ULg)
Full Text
See detailAdaptation of a Murine Chronic GVH Model to Study Graft versus Myeloma Effect after Allogeneic Transplantation
Binsfeld, Marilène ULg; Belle, Ludovic ULg; Hannon, Muriel ULg et al

in Belgian Journal of Hematology (2012), Abstracts book(Supplement of 27th General Meeting of the Belgian Hematological Society), 16

Detailed reference viewed: 6 (4 ULg)
Full Text
Peer Reviewed
See detailLymphome du manteau : prise en charge 2011
Bonnet, Christophe ULg; CAERS, Jo ULg; DE PRIJCK, Bernard ULg et al

in Revue Médicale Suisse (2011), 7

Le lymphome du manteau (LM) représente 6% des lymphomes non hodgkiniens (LNH). Le diagnostic repose sur l'immunophénotypage et la démonstration de la présence de la location entre les chromosomes 11 et 14 ... [more ▼]

Le lymphome du manteau (LM) représente 6% des lymphomes non hodgkiniens (LNH). Le diagnostic repose sur l'immunophénotypage et la démonstration de la présence de la location entre les chromosomes 11 et 14, avec surexpression de la cycline D1. Le traitement de première ligne du sujet jeune associe trois cures de R-CHOP21 alternées avec trois cures de R-DHAP21, suivies d'une autogreffe conditionnée par irradiation corporelle totale, cyclophosphamide et aracytine. Le sujet de plus de 65 ans peut bénéficier de huit cures de R-CHOP21. L'intérêt du traitement de maintenance est en cours d'évaluation. L'allogreffe de cellules souches hématopoïétiques offre une chance de guérison aux patients en rechute en bon état général. Les traitements ciblés permettront une amélioration du pronostic de cette maladie. [less ▲]

Detailed reference viewed: 53 (4 ULg)
Full Text
Peer Reviewed
See detailBiological aspects of angiogenesis in multiple myeloma.
Otjacques, Eléonore ULg; Binsfeld, Marilène ULg; Noël, Agnès ULg et al

in International Journal of Hematology (2011), 94(6), 505-18

Multiple myeloma (MM) is a hematological malignancy characterized by the aberrant expansion of malignant plasma cells within the bone marrow (BM). One of the hallmarks of this disease is the close ... [more ▼]

Multiple myeloma (MM) is a hematological malignancy characterized by the aberrant expansion of malignant plasma cells within the bone marrow (BM). One of the hallmarks of this disease is the close interaction between myeloma cells and neighboring cells within the BM. Angiogenesis, through the activation of endothelial cells, plays an essential role in MM biology. In the current review, we describe the angiogenesis process in MM by identifying the interacting cells, the pro- and anti-angiogenic cytokines modulated, and the extracellular matrix degrading proteases liable to participate in the pathophysiology. Finally, we highlight the impact of hypoxia (through hypoxia-inducible factor-1) and constitutive activation of nuclear factor-κB in this tumor-induced neo-vascularization. [less ▲]

Detailed reference viewed: 28 (6 ULg)
Full Text
Peer Reviewed
See detailPrise en charge actuelle des syndromes myélodysplasiques
CAERS, Jo ULg; BONNET, Christophe ULg; GRAUX, Carlos et al

in Revue Médicale Suisse (2011), 7

Detailed reference viewed: 15 (6 ULg)
Full Text
Peer Reviewed
See detailDiffuse xanthomatosis as a presenting feature of multiple myeloma.
Segner, Sophie; Theate, Ivan; Poire, Xavier et al

in European Journal of Haematology (2010), 84

Detailed reference viewed: 15 (3 ULg)
Full Text
Peer Reviewed
See detailRetroperitoneal fibrosis and multiple myeloma: fortuitous association?
Sinapi, Isabelle; Caers, Jo ULg; Connerotte, Thierry et al

in Revue de Médecine Interne (2010), 31(5), 4-6

Detailed reference viewed: 52 (1 ULg)
Full Text
Peer Reviewed
See detailThe effects of forodesine in murine and human multiple myeloma cells
Bieghs, Liesbeth; Caers, Jo ULg; De Bruyne, Elke et al

in Advances in Hematology (2010)

Detailed reference viewed: 20 (4 ULg)
Full Text
Peer Reviewed
See detailEnteroviral meningoencephalitis as complication of Rituximab therapy in a patient treated for diffuse large B-cell lymphoma
Servais, Sophie ULg; Caers, Jo ULg; Warling, Odette et al

in British Journal of Haematology (2010), 150(3), 379-381

Detailed reference viewed: 19 (6 ULg)
Full Text
Peer Reviewed
See detailThymosin Beta 4 has tumor suppressive effects and its decreased expression results in poor prognosis and decreased survival in multiple myeloma
Caers, Jo ULg; Hose, Dirk; Kuijpers, Ine et al

in Haematologica (2010), 95(1), 163-167

Detailed reference viewed: 16 (1 ULg)
Full Text
Peer Reviewed
See detailThymosin beta4 in multiple myeloma: friend or foe
Caers, Jo ULg; Otjacques, Eléonore ULg; Hose, Dirk et al

in Annals of the New York Academy of Sciences (2010), 1194(1), 125-130

Detailed reference viewed: 21 (6 ULg)
Peer Reviewed
See detailDecreased Thymosin Beta 4 Expression Results in Poor Prognosis and Decreased Survival in Multiple Myeloma
CAERS, Jo ULg; Hose, Dirk; Kuipers, Ine et al

Poster (2008)

Detailed reference viewed: 13 (1 ULg)
Full Text
Peer Reviewed
See detailUnraveling the biology of MM disease: cancer stem cells, acquired intracellular changes and interactions with the surrounding micro-environment.
Caers, Jo ULg; Van Valckenborgh, Els; Menu, Eline et al

in Bulletin du Cancer (2008), 95(3), 301-13

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailAntitumor and anti-angiogenic effects of aplidin in the 5T33MM model, syngeneic model of multiple myeloma
Caers, Jo ULg; De Raeve, Hendrik; Lepage, Doreen et al

in British Journal of Cancer (2008), 98(12), 1966-74

Detailed reference viewed: 9 (0 ULg)
Full Text
Peer Reviewed
See detailMultiple Myeloma, an update on diagnosis and treatment.
Caers, Jo ULg; Vande Broek, Isabelle; De Raeve, Hendrik et al

in European Journal of Haematology (2008), 81(5), 329-343

Detailed reference viewed: 7 (3 ULg)
Full Text
Peer Reviewed
See detailNeighboring adipocytes participate in the bone marrow microenvironment of multiple myeloma cells
CAERS, Jo ULg; Deleu, Sara; Belaid, Zakia et al

in Leukemia : Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K (2007), 21(7), 1580-4

Detailed reference viewed: 11 (1 ULg)