References of "Roland, Isabelle"
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See detailModulation of the arachidonic cascade with omega 3 fatty acids or analogues: Potential therapeutic benefits
Roland, Isabelle ULg; de Leval, X.; Evrard, Brigitte ULg et al

in Mini-Reviews in Medicinal Chemistry (2004), 4(6), 659-668

Increasing interest in the role of omega 3 fatty acids has arisen in these latest years since evidence of their implication in the cardioprotective fish based diet of the Inuit has been demonstrated ... [more ▼]

Increasing interest in the role of omega 3 fatty acids has arisen in these latest years since evidence of their implication in the cardioprotective fish based diet of the Inuit has been demonstrated. Furthermore, several in vitro, in vivo and epidemiological studies support the benefit of this fatty acids intake in various pathological states such as in the cardiovascular, cancer, inflammation, psychiatric, paediatric, pulmonary, dermatological and ophthalmologic fields. This review will focus on metabolism and pharmacological implication of w3 fatty acids intake as well as its interest in the prevention or treatment of the above-mentioned pathologies. [less ▲]

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See detailPreparation and in vivo toxicity study of solid lipid microparticles as carrier for pulmonary administration
Sanna, V.; Kirschvink, Nathalie; Gustin, Pascal ULg et al

in AAPS PharmScitech (2004), 5(2), 27

The purpose of this research was to investigate the effects of processing conditions on the characteristics of solid lipid microparticles (SLM) with a potential application as carriers for pulmonary ... [more ▼]

The purpose of this research was to investigate the effects of processing conditions on the characteristics of solid lipid microparticles (SLM) with a potential application as carriers for pulmonary administration. Compritol ( 5.0% wt/ wt) SLM dispersions were prepared by rotor-stator homogenization, at different surfactant concentrations and emulsification times. The SLM were characterized, in terms of morphology and size, after lyophilization and sterilization by autoclaving process. In vivo assessment was carried out in rats by intratracheal instillation of either placebo or SLM dispersion, and by bronchoalveolar lavage for cytological analysis. Mean particle size of 4 to 5 mum was achieved using 0.3% and 0.4% ( wt/ wt) of emulsifier ( Poloxamer 188) and emulsification times of 2 and 5 minutes. The particles showed spherical shape and smooth surface. The morphology of microparticles, the size, and the size distribution were not substantially modified after lyophilization and sterilization. Total cell counts showed no significant differences between placebo and SLM 0.5% or 2.5% groups. Regarding cytology, percentage of polymorphonuclear neutrophils and macrophages did not significantly differ between groups. These results suggest that a single intratracheal administration of the SLMs does not induce a significant inflammatory airway response in rats and that the SLMs might be a potential carrier for encapsulated drug via the pulmonary route. [less ▲]

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See detailCharacterisation of a novel topical nanoemulsion vehicle with spreading properties
Roland, Isabelle ULg; Gillard, Patricia ULg; Delattre, Luc ULg et al

in Proceedings of Eur conference on drug delivery and pharmaceutical technology (2004)

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See detailSystematic characterization of oil-in-water emulsions for formulation design
Roland, Isabelle ULg; Piel, Géraldine ULg; Delattre, Luc ULg et al

in International Journal of Pharmaceutics (2003), 263(1-2), 85-94

Oil-in-water emulsions varying in surfactant concentration and manufacturing process were prepared. About 10 experiments were performed to characterize them. The goal of this research was to find Out ... [more ▼]

Oil-in-water emulsions varying in surfactant concentration and manufacturing process were prepared. About 10 experiments were performed to characterize them. The goal of this research was to find Out which tests should systematically be carried Out to assess efficiently the stability and the properties of ail emulsified preparation. Thus, formulation design requires at least the measurement of the droplet size, the determination of the zeta potential, a TurbiScan(R) analysis, the investigation of the stability under centrifugation and freeze/thaw cycles. If the emulsion contains an active substance, stability under storage at 4 degreesC and microscopic analysis are relevant. Quality Control Should be improved by measurements of viscosity and pH. (C) 2003 Elsevier B.V. All rights reserved. [less ▲]

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See detailSolid lipid Nanoparticles for drug Pumonary Administration
Sanna, Vanna; Kirschvink, Nathalie; Gustin, Pascal ULg et al

in Proceedings of 30th International Symposium on Controlled Release of Bioactive Materials (2003)

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See detailFormulation and characterisation of submicronic emulsions
Roland, Isabelle ULg; Evrard, Brigitte ULg; Erpicum, Thomas et al

Conference (2001)

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See detailFormulation of subcronic emulsions by using a high-pressure homogeniser
Roland, Isabelle ULg; Evrard, Brigitte ULg; Erpicum, Thomas et al

Conference (2001)

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See detailHypoxia-induced activation of HIF-1: role of HIF-1alpha-Hsp90 interaction.
Minet, E.; Mottet, Denis ULg; Michel, G. et al

in FEBS Letters (1999), 460(2), 251-6

The protein chaperone heat shock protein 90 (Hsp90) is a major regulator of different transcription factors such as MyoD, a basic helix loop helix (bHLH) protein, and the bHLH-Per-aryl hydrocarbon nuclear ... [more ▼]

The protein chaperone heat shock protein 90 (Hsp90) is a major regulator of different transcription factors such as MyoD, a basic helix loop helix (bHLH) protein, and the bHLH-Per-aryl hydrocarbon nuclear translocator (ARNT)-Sim (PAS) factors Sim and aryl hydrocarbon receptor (Ahr). The transcription factor hypoxia-inducible factor-1alpha (HIF-1alpha), involved in the response to hypoxia, also belongs to the bHLH-PAS family. This work was aimed to investigate the putative role of Hsp90 in HIF-1 activation by hypoxia. Using a EGFP-HIF-1alpha fusion protein, co-immunoprecipitation experiments evidenced that the chimeric protein expressed in COS-7 cells interacts with Hsp90 in normoxia but not in hypoxia. We also demonstrated that Hsp90 interacts with the bHLH-PAS domain of HIF-1alpha. Moreover, Hsp90 is not co-translocated with HIF-1alpha into the nucleus. At last, we showed that Hsp90 activity is essential for HIF-1 activation in hypoxia since it is inhibited in the presence of geldanamycin. These results indicate that Hsp90 is a major regulator in HIF-1alpha activation. [less ▲]

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