References of "Ramery, Eve"
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See detailExpression microarray as a tool to identify candidate blood biomarkers in horses suffering from inflammatory airway disease
Ramery, Eve ULg; Fraipont, Audrey ULg; Art, Tatiana ULg et al

in Veterinary Clinical Pathology (in press)

Background: Inflammatory airway disease (IAD) affects performance and well-being in horses. Diagnosis is primarily reached by bronchoalveolar lavage (BAL) cytology but this is invasive and requires ... [more ▼]

Background: Inflammatory airway disease (IAD) affects performance and well-being in horses. Diagnosis is primarily reached by bronchoalveolar lavage (BAL) cytology but this is invasive and requires sedation. Objectives: The purpose of this study was to identify candidate blood biomarkers of IAD using species-specific expression microarrays. Methods: Horse Gene Expression Microarrays were used to investigate global mRNA expression in circulating leukocytes from healthy and IAD-affected standardbreds and endurance horses. Results: Nine genes were significantly differentially regulated in standardbreds and 61 in endurance horses (P < 0.001). These genes were mainly related to inflammation (eg. ALOX15B, PLA2G12B and PENK), oxidant/antioxidant balance (eg. DUOXA2 and GSTO1-1) and stress (eg. V1aR, GRLF1, Homer-2 and MAOB). DUOXA2, ALOX15B, PLA2G12B, MAOB and GRLF1 variations of expression were further validated by RT-qPCR. The deregulation of the oxidant/antioxidant balance was demonstrated at the protein level by an increase of glutathione peroxidase (GPx) activity in heparinised whole blood of IAD-affected standardbreds (P = 0.0025) and endurance horses (P = 0.0028). There was good correlation (r = 0.7354) between BAL neutrophil percentage and whole blood GPx activity in all horses. Conclusions: There is accumulating evidence that, even when systemic clinical signs are not evident, circulating leukocyte gene expression can reflect responses of other tissues, leading to potential diagnostic applications in the future. Although not specific for IAD, whole blood GPx activity appears to reflect BAL neutrophil percentage. This finding should be further assessed by testing a larger number of horses. [less ▲]

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See detailRasa3 Controls Megakaryocyte Rap1 Activation, Integrin Signaling and Differentiation into Proplatelet
Molina Ortiz, Patricia ULg; Polizzi, Séléna; Ramery, Eve ULg et al

in PLoS Genetics (2014), 10(6), 1004420

Rasa3 is a GTPase activating protein of the GAP1 family which targets Ras and Rap1. Ubiquitous Rasa3 catalytic inactivation in mouse results in early embryonic lethality. Here, we show that Rasa3 ... [more ▼]

Rasa3 is a GTPase activating protein of the GAP1 family which targets Ras and Rap1. Ubiquitous Rasa3 catalytic inactivation in mouse results in early embryonic lethality. Here, we show that Rasa3 catalytic inactivation in mouse hematopoietic cells results in a lethal syndrome characterized by severe defects during megakaryopoiesis, thrombocytopenia and a predisposition to develop preleukemia. The main objective of this study was to define the cellular and the molecular mechanisms of terminal megakaryopoiesis alterations. We found that Rasa3 catalytic inactivation altered megakaryocyte development, adherence, migration, actin cytoskeleton organization and differentiation into proplatelet forming megakaryocytes. These megakaryocyte alterations were associated with an increased active Rap1 level and a constitutive integrin activation. Thus, these mice presented a severe thrombocytopenia, bleeding and anemia associated with an increased percentage of megakaryocytes in the bone marrow, bone marrow fibrosis, extramedular hematopoiesis, splenomegaly and premature death. Altogether, our results indicate that Rasa3 catalytic activity controls Rap1 activation and integrin signaling during megakaryocyte differentiation in mouse. [less ▲]

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See detailMeasurement of cerebrospinal fluid albumin in healthy dogs
Ramery, Eve ULg; Girod, Maud ULg; Allerton, Fergus et al

in Veterinary Clinical Pathology (2013)

Background: Measurement of CSF albumin aids diagnosis in human medicine but technical difficulties related to its low CSF concentration prohibit its routine use in veterinary medicine. High-resolution ... [more ▼]

Background: Measurement of CSF albumin aids diagnosis in human medicine but technical difficulties related to its low CSF concentration prohibit its routine use in veterinary medicine. High-resolution electrophoresis (HRE) has been described but often results in non-interpretable integration profiles preventing albumin determination. Fraction quantification using HRE may be more precise after concentration (cHRE) using a membrane microconcentrator technique but has not been evaluated in CSF with total protein levels below 20mg/dL. Immunoturbidimetry is routinely used for human CSF albumin measurement and was recently applied on canine samples with encouraging results. Objective: The purpose of this study was to compare HRE (including the use of a concentration step) and immunoturbidimetric assay for the measurement of albumin levels in normal canine CSF. Methods: 30 CSF specimens from 15 healthy dogs were evaluated. CSF total protein was measured by the pyrogallol red methoda and CSF albumin was determined by HREb (n=15), cHREc (n=30) and immunoturbidimetric assayd (n=30). Validation of the human immunoturbidimetric assay was performed using a purified canine albumin standarde. Results: Mean CSF total protein was 17.5 (range 7-39) mg/dL. HRE integration profiles were non-interpretable in all unconcentrated specimens. However, clear distinction of the major protein fractions was achieved for all cHRE specimens. CSF albumin levels were measureable in 29/30 specimens using immunoturbidimetry. Excellent correlation (Pearson r=0.92, p<0.001) was found between the two techniques. Conclusion: Immunoturbidimetry and cHRE may be used for routine measurement of CSF albumin. [less ▲]

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See detailMeasurement of serum albumin by protein electrophoresis and bromocresol green methods in canine and equine patients
Ramery, Eve ULg; Bureau, Fabrice ULg

in Veterinary Clinical Pathology (2012, December), 41(4), 5

Background: Bromocresol green method (BCG) is routinely used in veterinary laboratories to measure albumin. A good agreement between BCG and electrophoresis (SPE) in healthy domestic animals is reported ... [more ▼]

Background: Bromocresol green method (BCG) is routinely used in veterinary laboratories to measure albumin. A good agreement between BCG and electrophoresis (SPE) in healthy domestic animals is reported. However, in human medicine, at low albumin and high globulin concentrations, BCG also binds to α- and β-globulins fractions. Surprisingly, there is no recent literature evaluating the BCG method in diseased domestic animals. Objective: The purpose of the present study was therefore to compare the serum albumin values obtained by SPE and BCG in canine and equine patients. Methods: Albumin measured by BCG and SPE was compared in the serum from 30 canine and 30 equine patients. Results: Good correlation was found (Spearman´s rho rs = 0.91 in dogs and 0.84 in horses). However, Altman and Bland analysis of results showed a positive bias of BCG with SPE (3.41 g/l in horses and 0.41 g/l in dogs) that increased when only hypoalbuminemic patients were considered (4.17 g/l in horses and 1.31 g/l in dogs). Conclusion: Results confirm that, despite good correlation, BCG method tends to overestimate albumin, especially in hypoalbuminemic patients. This is particularly evident in horses. [less ▲]

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See detailSuccessful reduction of cholelithiasis in a holstein cow
Lamain, Guillaume; Frisee, Vincent ULg; Ramery, Eve ULg et al

in Cattle Practice (2012), 20(1), 93-98

A pregnant 3.5 year-old high-productive Holstein cow in the third month of lactation showed colic signs, marked anorexia, and stopped milk production. Clinical examination revealed tachypnea, pyrexia and ... [more ▼]

A pregnant 3.5 year-old high-productive Holstein cow in the third month of lactation showed colic signs, marked anorexia, and stopped milk production. Clinical examination revealed tachypnea, pyrexia and the mucous membranes were all congested. Signs of abdominal discomfort were observed, the abdomen was tense and painful; digestive activity was poor. Blood investigations revealed inflammation, cholestasis, and leucocytosis with marked neutrophilia. Trans-abdominal ultrasonography revealed decreased digestive transit and enlarged gall-bladder. Hepatic or biliary involvement was suspected. A right-flank exploratory laparotomy revealed a gallbladder filled with firm and mobile 0.5 to 1cm diameter fluctuant masses. Digital palpation allowed cholelith extraction through the cystic duct into the duodenum. After the surgery, the cow progressively regained a normal appetite and pain signs decreased. Blood samples analysed 10 days after the surgery still showed inflammation but to a lesser extent. Hepatic enzymes were markedly increased, indicating hepatic injury. Three months after the surgery, the cow was healthy and was inseminated. This case report suggests that cholelithiasis may be diagnosed and successfully treated in early stage of the disease in cattle, though this condition is rare in this species. This is the second report of manual cholelith extraction in a cow. [less ▲]

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See detailEvaluation of the cytotoxicity of organic dust components on thp1 monocytes-derived-macrophages using high content analysis
Ramery, Eve ULg; O'Brien, Peter James

in Environmental Toxicology (2012)

Organic dust contains pathogen-associated molecular patterns (PAMPs) which can induce significant airway diseases following chronic exposure. Mononuclear phagocytes are key protecting cells of the ... [more ▼]

Organic dust contains pathogen-associated molecular patterns (PAMPs) which can induce significant airway diseases following chronic exposure. Mononuclear phagocytes are key protecting cells of the respiratory tract. Several studies have investigated the effects of PAMPs and mainly endotoxins, on cytokine production. However the sub-lethal cytotoxicity of organic dust components on macrophages has not been tested yet. The novel technology of high content analysis (HCA) is already used to assess subclinical drug-induced toxicity. It combines the capabilities of flow cytometry, intracellular fluorescence probes, and image analysis and enables rapid multiple analyses in large numbers of samples. In the present study, HCA was used to investigate the cytotoxicity of the 3 major PAMPs contained in organic dust, ie. endotoxin (LPS), peptidoglycan (PGN) and β-glucans (zymosan) on THP-1 monocyte-derived macrophages. LPS was used at concentrations of 0.005, 0.01, 0.02, 0.05, 0.1 and 1 μg/ml; PGN and zymosan were used at concentrations of 1, 5, 10, 50, 100 and 500 μg/ml. Cells were exposed to PAMPs for 24 h. In addition, the oxidative burst and the phagocytic capabilities of the cells were tested. An overlap between PGN intrinsic fluorescence and red/far-red fluorescent dyes occurred, rendering the evaluation of some parameters impossible for PGN. LPS induced sub-lethal cytotoxicity at the lowest dose (from 50 ng/ml). However, the greatest cytotoxic changes occurred with zymosan. In addition, zymosan, but not LPS, induced phagosome maturation and oxidative burst. Given the fact that β-glucans can be up to 100-fold more concentrated in organic dust than LPS, these results suggest that β-glucans could play a major role in macrophage impairment following heavy dust exposure and will merit further investigation in the near future. [less ▲]

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See detailAssessing fitness in endurance horses
Fraipont, Audrey ULg; Van Erck, Emmanuelle; Ramery, Eve ULg et al

in Canadian Veterinary Journal = Revue Vétérinaire Canadienne (2012), 53

A field test and a standardized treadmill test were used to assess fitness in endurance horses. These tests discriminated horses of different race levels: horses participating in races of 120km and more ... [more ▼]

A field test and a standardized treadmill test were used to assess fitness in endurance horses. These tests discriminated horses of different race levels: horses participating in races of 120km and more showed higher values of VLA4 (velocity at which blood lactate reached 4 mmol/L) and V200 (velocities at which heart rates reached 200 beats per min) than horses of lower race levels. [less ▲]

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See detailBacterial cholangiohepatitis in a dog
Ramery, Eve ULg; Papakonstantinou, Stratos; Pinilla, Manuel et al

in Canadian Veterinary Journal = Revue Vétérinaire Canadienne (2012), 53(4), 423-425

A 9-year-old female Yorkshire terrier was presented for vomiting and diarrhoea. Blood chemistry tests revealed hepatic dysfunction, cholestasis and inflammation. Liver ultrasonography and liver biopsy ... [more ▼]

A 9-year-old female Yorkshire terrier was presented for vomiting and diarrhoea. Blood chemistry tests revealed hepatic dysfunction, cholestasis and inflammation. Liver ultrasonography and liver biopsy were consistent with cholangiohepatitis. Fine needle aspiration of the gall-bladder revealed the presence of bacteria later identified as Clostridium spp. The cholangiohepatitis diagnosed was successfully treated. [less ▲]

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See detailEvaluation of organic dust components cytotoxicity on thp1 monocytes-derived-macrophages using high content analysis
Ramery, Eve ULg; O'Brien, Peter James

in Veterinary Clinical Pathology (2011, December), 40(4), 583

Background: Organic dust contains pathogen associated molecular patterns (PAMPs) which can induce, following chronic exposure, significant airway diseases. Mononuclear phagocytes are key protecting cells ... [more ▼]

Background: Organic dust contains pathogen associated molecular patterns (PAMPs) which can induce, following chronic exposure, significant airway diseases. Mononuclear phagocytes are key protecting cells of the respiratory tract. Several studies have investigated the effects of PAMPs, and mainly endotoxins, on cytokine production. However the sub-lethal cytotoxicity of organic dust components on macrophages has not been tested yet. The novel technology of high content analysis (HCA) is already used to assess subclinical drug-induced toxicity. It combines the capabilities of flow cytometry, intracellular fluorescence probes, and image analysis and enables to perform rapid multiple analysis in large numbers of samples. Objectives: The purpose of the present study was, by using HCA, to investigate the cytotoxicity of the 3 major PAMPs contained in organic dust, ie. endotoxin (LPS), peptidoglycan (PGN) and β-glucans (zymosan) on THP-1 monocyte-derived macrophages. Methods: LPS was used at concentrations of 0.005, 0.01, 0.02, 0.05, 0.1 and 1 μg/ml; PGN and zymosan were used at concentrations of 1, 5, 10, 50, 100 and 500 μg/ml. Cells were exposed to PAMPs for 24 hours. In addition, the oxidative burst and the phagocytic capabilities of the cells were tested. Results: An overlap between PGN intrinsic fluorescence and red/far-red fluorescent dyes occurred, rendering the evaluation of some parameters impossible for PGN. LPS induced sub-lethal cytotoxicity at the lowest dose (from 10 ng/ml). However, the most spectacular changes occurred with zymosan. In addition, zymosan, but not LPS, induced phagosome maturation and oxidative burst. Conclusions: Given the fact that β-glucans can be up to 100 fold more concentrated in organic dust than LPS, these results suggest that β-glucans could play a major role in macrophages impairment following heavy dust exposure and will deserve further investigation in the near future. [less ▲]

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See detailExercise and epinephrine affect equine leukocytes distribution and gene expression in a distinct but closely related pattern
Ramery, Eve ULg; Schoenecker, Julie; Art, Tatiana ULg et al

in Veterinary Clinical Pathology (2011, December), 40(4), 583-584

Background: In horses-athletes, exercise has been shown to be an important regulator of immune cells and their functions, and catecholamines to be essential components of the physiological response to ... [more ▼]

Background: In horses-athletes, exercise has been shown to be an important regulator of immune cells and their functions, and catecholamines to be essential components of the physiological response to exercise. Objectives: The purpose of the present study was to compare immunological reactions and molecular events during exercise and epinephrine infusion. Methods: Horses were submitted to exercise or epinephrine infusion for 10 min. Blood samples were collected at T0, T5, Tl0, T20 and T40 min for biochemistry and haematology at T0 and T20 min for RNA extraction and microarray analysis. Results: In both protocols, packed-cell-volume and neutrophils peaked at T5 and T10, and returned to baseline at T40. Lymphocytes increased at T5 with exercise and at T20 with epinephrine and then remained high until the end of the protocols. Between T0 and T20, 55 probes, coding for 25 genes, and 19 probes, coding for 11 genes, were differentially regulated with epinephrine and exercise respectively (P<0.001). Ten of the genes over-expressed with epinephrine were related to inflammatory response and leukocytes trafficking and activation. Among the genes with the highest significant fold changes following exercise were FOS, DUSP-1 and CXCL1. FOS was over-expressed in both conditions. Among genes differentially regulated, Ingenuity software identified 3 overlapping functional networks with epinephrine and 1 functional network with exercise that overlapped the 3 epinephrine networks. Conclusions: Circulating leukocytes distribution and gene expression are rapidly affected following exercise and epinephrine infusion. Distinct but closely-related sets of gene activities were shown to be altered with exercise and epinephrine, with lesser changes in the immunological pattern with physical stress than epinephrine alone, arguing for compensatory mechanisms in exercise. [less ▲]

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See detailKyste hématique valvulaire chez une vache Holstein
Lamain, Guillaume ULg; Ramery, Eve ULg; Guyot, Hugues ULg

in Point Vétérinaire (2011), 320

Une vache laitière haute productrice est présentée pour une fièvre récidivante. L’animal présente des symptômes cardiaques et digestifs. Un phénomène inflammatoire et une atteinte cardiaque sont mis en ... [more ▼]

Une vache laitière haute productrice est présentée pour une fièvre récidivante. L’animal présente des symptômes cardiaques et digestifs. Un phénomène inflammatoire et une atteinte cardiaque sont mis en évidence. Une endocardite bactérienne est suspectée. L’autopsie de la vache révèle l’existence d’un kyste hématique sur la valve tricuspide et une péritonite localisée ayant pour point de départ un abcès périruménal. [less ▲]

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See detailSuccessful reduction of cholelithiasis in a Holstein cow
Lamain, Guillaume ULg; Frisee, Vincent ULg; Ramery, Eve ULg et al

Poster (2011, September)

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See detailAccuracy of inflammation field tests in cattle practice
Guyot, Hugues ULg; Lamain, Guillaume ULg; Detilleux, Johann ULg et al

in Société Belge Francophone de Buiatrie & Vlaamse vereniging voor Buiatrie (Ed.) Proceedings of the 6th European Congress of Bovine Health Management (2011, September)

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See detailOutils diagnostiques des maladies infectieuses utilisables en ferme
Guyot, Hugues ULg; Ramery, Eve ULg

in Point Vétérinaire (2011), N° Spécial: Maladies Infectieuses des Ruminants: Actualités(314), 78-84

La médecine vétérinaire se développe et s’équipe d’outils de terrain précis pour diagnostiquer la présence d’infections voire même d’en déterminer l’agent étiologique. Des tests de terrain ont été conçus ... [more ▼]

La médecine vétérinaire se développe et s’équipe d’outils de terrain précis pour diagnostiquer la présence d’infections voire même d’en déterminer l’agent étiologique. Des tests de terrain ont été conçus pour pouvoir affiner les diagnostics au chevet du patient. Pour savoir si la maladie d’un bovin examiné a une origine infectieuse, on peu recourir à des tests mesurant l’inflammation, des tests indiquant la présence de cellules inflammatoires, des tests mettant en évidence des agents infectieux au sein de prélèvements biologiques et des tests mesurant les conséquences métaboliques du passage d’un agent infectieux. Par ailleurs, des méthodes de diagnostic de terrain ont été développées pour rechercher de manière spécifique des agents infectieux dans les selles (diarrhées), le lait (mammites), l’urine (infections du système urinaire), le sang (parasites sanguins), et enfin dans les poumons (broncho-pneumonies). Par ailleurs, il est possible de prévoir une morbidité et mortalité accrues chez les jeunes veaux en testant le transfert passif de l’immunité colostrale. La recherche d’un diagnostic étiologique en médecine vétérinaire des ruminants conduit à l’établissement de thérapeutiques ciblées. Il ne faut toutefois pas oublier que les laboratoires d’analyse restent les centres de référence pour confirmer la plupart des étiologies des maladies infectieuses chez les ruminants et autres espèces. [less ▲]

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See detailEffect of a combined iodine and selenium supplementation on I and Se status of cows and their calves
Guyot, Hugues ULg; Alves de Oliveira, Laurent; Ramery, Eve ULg et al

in Journal of Trace Elements in Medicine and Biology (2011), 25(2), 118-124

Iodine (I) and selenium (Se) deficiencies are commonly reported in cattle, however, there are also studies regarding a very high iodine supply. The aim of the study was to determine the long-term effect ... [more ▼]

Iodine (I) and selenium (Se) deficiencies are commonly reported in cattle, however, there are also studies regarding a very high iodine supply. The aim of the study was to determine the long-term effect of I and Se supplementation on non-pregnant cows, pregnant cows and their calves. The hypothalamus pituitary axis was investigated (TSH, T4, T3 assays) during a TRH challenge on non-pregnant cows. Twenty-four cows, half of them pregnant, were assigned into 2 diet-groups, one group with a low I (0.45 ppm) and Se (0.15 ppm) diet (LISe), the other with a high I (5.45 ppm) and Se (0.45 ppm) diet (HISe), for a period of 120 days. Nutritional (plasma iodide, urinary I, plasma Se, I content in colostrum and foetal fluids) and functional (thyrotropin, thyroid hormones, glutathione-peroxidase activity in erythrocytes) markers of I and Se status were assayed in dams at regular intervals for 120 days and in their calves at birth. A TRH challenge was performed on 8 non-pregnant cows at day 110 of the trial. At the end of the study, I and Se nutritional markers were higher in dams in the HISe group, compared to the LISe group, except for plasma Se. At birth, I nutritional markers in calves in the HISe group were higher compared to the LISe group. Reactivity of the pituitary–thyroid-axis was not influenced by I and Se supplementation. I and Se supplementation is efficient in improving newborn status. [less ▲]

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See detailLe microdamier d’expression : un outil performant dans la compréhension de la réponse
Schoeneker, Julie; Art, Tatiana ULg; Pirottin, Dimitri ULg et al

in Annales de Médecine Vétérinaire (2011), 154

Malgré de multiples études, les interrelations entre l’exercice, le stress et la réponse immune sont peu définies. Or, la compréhension de ces interrelations pourrait jouer un rôle très important dans ... [more ▼]

Malgré de multiples études, les interrelations entre l’exercice, le stress et la réponse immune sont peu définies. Or, la compréhension de ces interrelations pourrait jouer un rôle très important dans l’amélioration de la santé et des résultats sportifs des athlètes. En effet, l’exercice peut être reconnu comme un stress. Il induit des modifications de l’équilibre homéostatique qui peuvent à leur tour altérer la réponse immunitaire de l’hôte et donc sa susceptibilité aux maladies. L’adrénaline est la molécule essentielle de tout processus de stress. La technologie des microdamiers, outil majeur d’investigation transcriptomique, permet l’étude de l’expression génique de l’ensemble du génome. Son utilisation devrait donc permettre de mieux caractériser et définir les interrelations entre l’exercice, le stress et la réponse immune. Cette revue recense les interrelations connues entre la réponse immune à un stress adrénergique d’une part et la réponse immune à l’exercice d’autre part. Elle considère en outre la contribution potentielle des microdamiers à une meilleure compréhension des effets d’un stress, et plus particulièrement celui lié à l’exercice, sur l’immunité. [less ▲]

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See detailSubclinical diseases underlying poor performance in endurance horses: diagnostic methods and predictive tests.
Fraipont, Audrey ULg; Van Erck, E.; Ramery, Eve ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2011), 169(6), 154

Thirty-eight endurance horses underwent clinical and ancillary examinations, including haematological and biochemical evaluation, standardised exercise tests both on a treadmill and in the field, Doppler ... [more ▼]

Thirty-eight endurance horses underwent clinical and ancillary examinations, including haematological and biochemical evaluation, standardised exercise tests both on a treadmill and in the field, Doppler echocardiography, impulse oscillometry, video endoscopy and collection of respiratory fluids. All of the examined poorly performing horses were affected by subclinical diseases, and most of them had multiple concomitant disorders. On the contrary, the well-performing horses were free of any subclinical disease. The most frequently diagnosed diseases were respiratory disorders, followed by musculoskeletal and cardiac problems. Poor performers exhibited lower speeds at blood lactate concentration of 4 mmol/l (VLA4) and at heart rates of 160 (V160) and 200 bpm (V200) on the treadmill and in the field, as well as slower recovery of heart rate. [less ▲]

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