References of "Parrilla Hernandez, Sonia"
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See detailHigh concentrations of Myeloperoxidase in the equine uterus as an indicator of endometritis
Parrilla Hernandez, Sonia ULg; Ponthier, Jérôme ULg; Franck, Thierry ULg et al

in Theriogenology (in press)

Intra-luminal fluid and excessive abnormal hyper-edema are regularly used for the diagnosis of endometritis in the mare, which is routinely confirmed by the presence of neutrophils on endometrial smears ... [more ▼]

Intra-luminal fluid and excessive abnormal hyper-edema are regularly used for the diagnosis of endometritis in the mare, which is routinely confirmed by the presence of neutrophils on endometrial smears. Studies show a relation between neutrophils and myeloperoxidase (MPO), an enzyme contained in and released by neutrophils during degranulation or after cell lysis. This enzyme has been found in many fluids and tissues and associated with different inflammatory pathologies in the horse. The aims of this study were to assess the presence and concentration of MPO in the equine uterus, and to investigate its relation with neutrophils, and other clinical signs of endometritis. Mares (n=51) were evaluated for presence of intra-luminal fluid and excessive endometrial edema before breeding, and a small volume lavage and cytology samples were obtained. From 69 cycles, supernatant of the uterine flushes was analysed with a specific equine MPO ELISA assay to measure MPO concentration. Cytology samples were used for the diagnosis of endometritis. MPO was present in the uterus of all estrus mares in highly variable concentrations. MPO concentrations were significantly (p<0.05) higher in samples with positive cytologies and in presence of intra-luminal fluid. Occasionally, some samples with negative cytologies showed high MPO concentration, but the opposite was never observed. Cycles presenting hyper-edema weren’t associated to high concentration of MPO, intra-luminal fluid or positive cytology making it a poor diagnostic tool of endometritis. [less ▲]

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See detailConcentration, Activity and Biochemical Characterization of Myeloperoxidase in Fresh and Post-thaw equine semen and their Implication on Freezability
Ponthier, Jérôme ULg; Franck, Thierry ULg; Parrilla Hernandez, Sonia ULg et al

in Reproduction in Domestic Animals (in press)

Myeloperoxidase (MPO) is a pro-oxidant enzyme associated with decreased motility in thawed equine semen. This study aimed to describe MPO concentration, activity and subunits in raw and thawed semen and ... [more ▼]

Myeloperoxidase (MPO) is a pro-oxidant enzyme associated with decreased motility in thawed equine semen. This study aimed to describe MPO concentration, activity and subunits in raw and thawed semen and to correlate these data with motilities in raw and thawed semen. Semen samples from five stallions were collected four times. Motilities were assessed in raw and thawed semen. MPO assays were performed in raw seminal plasma, raw sperm-rich pellet and thawed semen. Total and active MPO concentrations were, respectively, assayed by enzyme-linked immunosorbent assay and specific immunological extraction followed by enzymatic detection. MPO subunits present in semen were characterized by Western 3 blot. Purified active MPO was added in PBS and freezing extender to control its activity during freezing procedure. Differences between medians were determined using Kruskal– Wallis test, and correlations were determined using Spearman’s test for nonparametric data. Active MPO concentration was low in seminal plasma and thawed semen, but high in pellet (p = 0.0058), as the opposite relation was observed for total MPO concentration (p < 0.0001). In seminal plasma and post- thaw semen, inactive 86-kDa MPO precursor was mainly observed. Purified MPO activity was decreased in the extender (p = 0.0286). MPO activity in pellet was highly correlated with thawed progressive motility (r = 􏰑0.5576, p = 0.0086). Inac- tive MPO precursor and unknown low molecular weight inactive MPO precursor subunits explain low MPO activity in semen. Major MPO activity was observed in pellet, and post- thaw loss of activity is partially explained by MPO inactiva- tion in extender. Thawed semen motility was negatively correlated with MPO activity in pellet, becoming a potential freezability predictor. [less ▲]

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See detailUse of automatic stapling device during castration to prevent (re)occurrence of inguinal hernia in horses with large vaginal rings.
Salciccia, Alexandra ULg; de la Rebière de Pouyade, Geoffroy ULg; Ponthier, Jérôme ULg et al

in Journal of Equine Veterinary Science (2014, January), 34(1), 99-100

(Re)ocurrence of inguinal hernia in case of very large vaginal rings may simply be prevented by closure of the vaginal tunic by TA staples during castration by inguinal approach. This method provides a ... [more ▼]

(Re)ocurrence of inguinal hernia in case of very large vaginal rings may simply be prevented by closure of the vaginal tunic by TA staples during castration by inguinal approach. This method provides a good resistance to internal pressure, appears to be safe, fast and easy to perform and may therefore be an interesting alternative to laparoscopic techniques when castration is considered. [less ▲]

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See detailSperm Motility and Lactate production at different sperm concentrations
Ponthier, Jérôme ULg; De Tullio, Pascal ULg; Blommaert, Didier ULg et al

in Journal of Equine Veterinary Science (2014, January), 34(1), 75-76

Lactate production is associated with total spermatozoa concentration. It negatively affects preservation of total and progressive motility, showing an effect of by-products of anaerobic metabolism on ... [more ▼]

Lactate production is associated with total spermatozoa concentration. It negatively affects preservation of total and progressive motility, showing an effect of by-products of anaerobic metabolism on long-term storage. Moreover, our data show that non-progressive motile spermatozoa are highly associated to lactate concentration, and thus, anaerobic glycolysis. More studies are required to determine relative contributions of aerobiosis and anaerobiosis to spermatozoa motility under different storage conditions. [less ▲]

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See detailPyovagina caused by pyometra and a persistent hymen in a 9-year-old bitch
Van Den Berghe, Femke ULg; Ponthier, Jérôme ULg; Parrilla Hernandez, Sonia ULg et al

in Reproduction in Domestic Animals (2013, September), 48(suppl1), 114

Pyovagina with a persistent hymen is very uncommon in a 9-year-old intact bitch. Blood discharge during prooestrus phases of previous cycles and the recent purulent vulvar discharge indicate an -at least ... [more ▼]

Pyovagina with a persistent hymen is very uncommon in a 9-year-old intact bitch. Blood discharge during prooestrus phases of previous cycles and the recent purulent vulvar discharge indicate an -at least partially- perforated hymen. For an unknown cause the hymen must have re-sealed after uterine contamination explaining a pyovagina that was bulging in the perineal area [less ▲]

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See detailInfluence of concentration on equine fresh semen conservation
Ponthier, Jérôme ULg; Bloomaert, Didier; Parrilla Hernandez, Sonia ULg et al

in Reproduction in Domestic Animals (2013, September), 48(suppl1), 85

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See detailA case of Longitudinal Vaginal Septum in a mare
Parrilla Hernandez, Sonia ULg; Ponthier, Jérôme ULg; Deleuze, Stefan ULg

in Reproduction in Domestic Animals (2013, September), 48(suppl1), 112-113

LVS occurs when there is failure in the fusion of müllerian ducts or in the regression of the vaginal septum. Primary or secondary cause of infertility, increases the risk of dystocia or alter sport ... [more ▼]

LVS occurs when there is failure in the fusion of müllerian ducts or in the regression of the vaginal septum. Primary or secondary cause of infertility, increases the risk of dystocia or alter sport performance. It is most likely under-diagnosed as it is often asymptomatic and an incidental finding. [less ▲]

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See detailMyeloperoxidase activity decreases in equine semen freezing extenders
Ponthier, Jérôme ULg; Franck, Thierry ULg; Niesten, Ariane ULg et al

in Amir, Arav (Ed.) Proceedings of the 3rd Cryo Congress (2013, March 23)

Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutrophils, and associated with decreased post-thaw motility of equine semen. This study aimed to compare MPO activity in pure ... [more ▼]

Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutrophils, and associated with decreased post-thaw motility of equine semen. This study aimed to compare MPO activity in pure equine freezing extender, raw and post-thaw semen. Active MPO Concentration (AMC) was measured with Specific Immunologic Extraction Followed by Enzymatic Detection in 20 ejaculates. Raw semen intra cellular AMC was determined in the supernatant after membrane lysis, each pellet containing 100x106 spermatozoa. AMC was also assayed in supernatants of semen frozen following a conventional method using INRA FreezeTM (IMV, France). Effect of freezing procedure on AMC was tested by experimentally adding 500ng of purified active MPO (Calbiochem, Germany) in 4 samples either with 5ml of PBS or INRA FreezeTM before assay. AMC was higher in sperm-rich pellet (0.306ng/ml) than in post-thaw semen (0.002ng/ml) (p=0.058). After experimental MPO addition, no activity variation was observed during the freezing procedure (after dilution, 1, 2 hours of cooling and post-thawing) within the same medium. Purified MPO activity was decreased in INRA FreezeTM when compared to PBS at all timings of sampling (p=0.0286). When all samples were pooled, remaining activity in INRA FreezeTM was 23.93±13.13%. MPO fixation on large proteins contained in the extender experimentally reduces AMC, as previously observed in plasma. However, AMC decrease observed during semen freezing is more important than after experimental addition. That could be explained by a MPO interaction with seminal plasma, a partial MPO release or a MPO inactivation during equine semen freezing. [less ▲]

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See detailINFLUENCE OF MYELOPEROXIDASE ACTIVITY ON EQUINE POST-THAW SEMEN QUALITY
Ponthier, Jérôme ULg; Franck, Thierry ULg; Parrilla Hernandez, Sonia ULg et al

in Edeas, Marvin (Ed.) Proceedings of the 2nd ISANH World congress on Fertility and Antioxidants (2012, December 06)

This study confirms that active MPO is associated with cellular fraction of the ejaculate, as previously suggested for total MPO concentration in thawed semen (2). However, active MPO concentrations were ... [more ▼]

This study confirms that active MPO is associated with cellular fraction of the ejaculate, as previously suggested for total MPO concentration in thawed semen (2). However, active MPO concentrations were dramatically lower than total MPO concentrations observed in equine semen (3), which could be explained by presence of inactive MPO subunits in semen. MPO activity in sperm-rich pellet can be used as a predictive marker of post-thaw semen quality. Moreover, methods to inhibit MPO should be investigated in semen. [less ▲]

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See detailA flow cytometric study on the effect of myeloperoxidase on stallion spermatozoal motility and structure
Ponthier, Jérôme ULg; Parrilla Hernandez, Sonia ULg; Van Den Berghe, Femke ULg et al

in Journal of Equine Veterinary Science (2012, August), 32(8), 509

Myeloperoxidase (MPO) is a pro-oxidant enzyme that is normally contained in neutrophils. MPO has recently been associated with keratinized cells and with decreased post-thaw motility in stallion semen [1 ... [more ▼]

Myeloperoxidase (MPO) is a pro-oxidant enzyme that is normally contained in neutrophils. MPO has recently been associated with keratinized cells and with decreased post-thaw motility in stallion semen [1]. The aim of the study was to determine effects of experimental addition of active MPO on motility, mitochondrial potential, apoptosis induction, membrane and acrosome integrity in equine semen. Three stallions were used and semen was collected four times. Extended (INRA96TM) semen was processed for density gradient centrifugation (Equipure Bottom Layer®) [2]. Purified pellet was re-extended to 100x106spermatozoa/ml in INRA96TM and divided in 3 samples. One sample was used for control and active human MPO (Calbiochem, Merck) was added in the other two samples to final concentration of 5 or 50 ng/ml. After incubation (2 hours, 20°C), motility was analysed with Computer Assisted Semen Analysis (IVOS, Hamilton-Throne, Beverly, MA, USA) and cytometric analyzes were perfomed with EasyCyte (IMV). Mitochondrial potential and apoptosis were assayed using Guava Mitopotential JC-1 and 7-AAD kit (Millipore). Membrane and acrosome integrity were respectively assayed with PI (Propidium Iodide) (Invitrogen) and PNA (Peanut Agglutinin-Fluorescein Iso Thio Cyanate) (Sigma-Aldrich). Statistical differences (p<0.05) were determined using Kruskall-Wallis test. No effect of the stallions was observed on parameters assayed in this study. Unlike total motility, progressive motility was decreased in both MPO concentrations (p<0.001). MPO addition had no effect on membrane and acrosome integrity. No differences were detected for the percentages of spermatozoa having polarised or depolarised mitochondria. Apoptosis, assayed by 7-AAD fluorescence, was not increased by the treatments. Our results agree with previously published effects of in vitro ROS production systems with xanthine oxidase [3], showing an effect on motility but no influence on mitochondria and membrane or acrosome integrity. However, membrane lipoperoxidation was increased by ROS in this study [3], and it could be linked to the impaired motility also observed in our protocol. Further studies with increasing concentrations of added MPO should be conducted to correlate motility with lipoperoxidation. References [1] Ponthier J, Desvals M, Franck T, de la Rebiere de Pouyade G, Spalart M, Palmer E, Serteyn D, Deleuze S. Myeloperoxidase in equine semen: Concentration and Localization during freezing processing. Journal of Equine Veterinary Science 2012;32: 32-37. [2] Edmond AJ, Teague SR, Brinsko SP, Comerford KL, Waite JA, Mancill SS, Love CC, Varner DD. Effect of density-gradient centrifugation on quality and recovery rate of equine spermatozoa. Animal Reproduction Science 2008;107: 318-318. [3] Baumber J, Ball BA, Gravance CG, Medina V, Davies-Morel MC. The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential, and membrane lipid peroxidation. J Androl 2000;21: 895-902. [less ▲]

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See detailMyeloperoxidase as an indicator of endometritis in the mare: preliminary results
Parrilla Hernandez, Sonia ULg; Franck, Thierry ULg; Ponthier, Jérôme ULg et al

in Reproduction in Domestic Animals (2012, August), 47(suppl 5), 65

Diagnosis of endometritis in the mare is routinely based on the presence of polymorphonuclear cells (PMNs) on endometrial smears. Studies show a relation between PMNs and myeloperoxidase (MPO), an enzyme ... [more ▼]

Diagnosis of endometritis in the mare is routinely based on the presence of polymorphonuclear cells (PMNs) on endometrial smears. Studies show a relation between PMNs and myeloperoxidase (MPO), an enzyme released by PMNs during degranulation or after cell lysis, in many fluids and tissues. The aims of this study were to assess the presence and concentration of MPO in the mare’s uterus, and to investigate its relation with PMNs. Thirty-six cycles from 28 mares (ages ranging from 6 to 22 years) were used. Endometrial cytological samples were obtained with a small volume uterine flush and either a uterine cotton swab or a cytobrush, when a follicle >35 mm was observed by ultrasound. The smears were stained with Diff-QuickÒ and one or more PMNs per field (400·) was diagnosed as endometritis. The supernatant of the flushes was used to measure MPO concentration with a specific equine MPO ELISA assay. Our results showed the presence of MPO in the equine uterus during oestrus (mean = 2839 + 2785). MPO concentrations were signifi- cantly (p < 0.05) higher in samples with positive cytological results. Occasionally, some samples with negative cytological results showed high MPO concentration, but the opposite was never observed. Clinical signs of endometritis are not always present, or they may be delayed. An early diagnostic improves the success of treatment. Our results show that high quantities of MPO in endometrial samples indicate the presence of PMNs. Further studies are needed to determine if MPO concentration could be routinely used as a tool of early detection of endometritis. [less ▲]

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See detailEffect of artificial insemination site on post-mating endometritis in mares
Rodier, Clémentine; Ponthier, Jérôme ULg; Parrilla Hernandez, Sonia ULg et al

in Reproduction in Domestic Animals (2012, August), 47(suppl 5), 103

Aim of the study was to determine the effect of artificial insemination (AI) location on post-mating endometrial inflammation 1 and 6 days after AI. Six mares with ages ranging from 12 to 23 years were ... [more ▼]

Aim of the study was to determine the effect of artificial insemination (AI) location on post-mating endometrial inflammation 1 and 6 days after AI. Six mares with ages ranging from 12 to 23 years were inseminated with a same batch of frozen semen for 3 consecutive cycles. Mares were inseminated with the following procedures in a random order: (1) Deep uterine insemination with 4ml of semen; (2) Horn bifurcation with 4ml of semen; (3) Horn bifurcation with 4ml of semen and 6ml of extender. During each cycle, Cotton (C) and Brush (B) swabs were collected at 4 different moments: mid- dioestrus, mares with a 35mm follicle, 24h and 6 days after AI. Swabs were smeared on slides, fixed and stained (Diff-Quick®) before examination under light microscopy. Proportions of inflammatory and epithelial cells were determined and differences were studied with kruskal-wallis test for non-parametric data. Distensions of uterine lumen due to intraluminal fluid observed during ultrasound exams were measured and recorded. Quality of slides was better (p=0.0006) with B swabs than C swabs with 97% versus 65% of slides readable. B swabs were associated with higher percent of endometrial cells retrieved (p=0.0323), making them a better tool for endometritis diagnosis, which is consistent with our previous reports. Volume of intraluminal fluid and percent of inflammatory cells, both on B and C swabs, were not influenced by AI location regardless the timing of sampling. Small volume deep uterine AI did not significantly affect inflammatory response by the endometrium in our experiment. [less ▲]

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See detailRaw semen concentration directly influences CASA velocity pathways
Parrilla Hernandez, Sonia ULg; Deleuze, Stefan ULg; Van Den Berghe, Femke ULg et al

in Journal of Equine Veterinary Science (2012, August), 32(8), 505

We observed an influence of the stallion on volume, spermatozoa concentration and all CASA parameters (p<0.0001), which are highly dependant on each other due to geometrical association of these data ... [more ▼]

We observed an influence of the stallion on volume, spermatozoa concentration and all CASA parameters (p<0.0001), which are highly dependant on each other due to geometrical association of these data. That stallion effect may have interfered with the associations we observed as stallions seem to have specific concentration and motility pathways. More studies, with more replicates, will allow comparing results from a same stallion and further establish the correlations we report here. [less ▲]

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See detailLa spermatogenèse, la libido et la dose
Ponthier, Jérôme ULg; Lecrenier, Marie-Caroline; Parrilla Hernandez, Sonia ULg et al

Conference given outside the academic context (2010)

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