References of "Neirinckx, Virginie"
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See detailSpinal Cord Injuries – How Could Adult Mesenchymal and Neural Crest Stem Cells Take Up the Challenge?
Neirinckx, Virginie ULg; CANTINIEAUX, Dorothée ULg; Coste, Cécile ULg et al

in Stem Cells (2013)

Since several years, adult/perinatal mesenchymal and neural crest stem cells have been widely used to help experimental animal to recover from spinal cord injury. More interestingly, recent clinical ... [more ▼]

Since several years, adult/perinatal mesenchymal and neural crest stem cells have been widely used to help experimental animal to recover from spinal cord injury. More interestingly, recent clinical trials confirmed the beneficial effect of those stem cells, which improve functional score of patients suffering from such lesions. However, a complete understanding of the mechanisms of stem cell-induced recovery is seriously lacking. Indeed, spinal cord injuries gathered a wide range of biochemical and physiopathological events (such as inflammation, oxidative stress, axonal damage, demyelination, etc) and the genuine healing process after cell transplantation is not sufficiently defined. This review aims to sum up recent data about cell therapy in spinal cord lesions using mesenchymal or recently identified neural crest stem cells, by describing precisely which physiopathological parameter is affected and the exact processes underlying the observed changes. Overall, although significant advances are acknowledged, it seems that further deep mechanistic investigation is needed for the development of optimized and efficient cell-based therapy protocols. [less ▲]

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See detailAdult bone marrow stem cells in neurological diseases: Protect or repair?
Neirinckx, Virginie ULg

Conference (2013, September 12)

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See detailIn vitro and in vivo Characterization of Adult Bone Marrow Neural Crest Stem Cells
Coste, Cécile ULg; Neirinckx, Virginie ULg; Manguette, Jérôme et al

Poster (2013, May 31)

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See detailAdult Bone Marrow Neural Crest Stem Cells and Mesenchymal Stem Cells are not able to Replace Lost Neurons in Acute MPTP-lesioned Mice
Neirinckx, Virginie ULg; Marquet, Alice ULg; Coste, Cécile ULg et al

in PLoS ONE (2013), 8(5),

Adult bone marrow stroma contains multipotent stem cells (BMSC) that are a mixed population of mesenchymal and neural-crest derived stem cells. Both cells are endowed with in vitro multi-lineage ... [more ▼]

Adult bone marrow stroma contains multipotent stem cells (BMSC) that are a mixed population of mesenchymal and neural-crest derived stem cells. Both cells are endowed with in vitro multi-lineage differentiation abilities, then constituting an attractive and easy-available source of material for cell therapy in neurological disorders. Whereas the in vivo integration and differentiation of BMSC in neurons into the central nervous system is currently matter of debate, we report here that once injected into the striatum of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice, pure populations of either bone marrow neural crest stem cells (NCSC) or mesenchymal stem cells (MSC) survived only transiently into the lesioned brain. Moreover, they do not migrate through the brain tissue, neither modify their initial phenotype, while no recovery of the dopaminergic system integrity was observed. Consequently, we tend to conclude that MSC/NCSC are not able to replace lost neurons in acute MPTP-lesioned dopaminergic system through a suitable integration and/or differentiation process. Altogether with recent data, it appears that neuroprotective, neurotrophic and anti-inflammatory features characterizing BMSC are of greater interest as regards CNS lesions management. [less ▲]

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See detailAdult Mesenchymal Stem Cells, Adult Neural Crest Stem Cells and Therapy of Neurological Pathologies: a State of Play
Neirinckx, Virginie ULg; Coste, Cécile ULg; Rogister, Bernard ULg et al

in Stem Cells Translational Medicine (2013), 2(4), 284-296

Adult stem cells are endowed with in vitro multi-lineage differentiation abilities, and constitute an attractive autologous source of material for cell therapy in neurological disorders. With regards to ... [more ▼]

Adult stem cells are endowed with in vitro multi-lineage differentiation abilities, and constitute an attractive autologous source of material for cell therapy in neurological disorders. With regards to lately published results, the ability of adult mesenchymal stem cells (MSC) and neural crest stem cells (NCSC) to integrate and differentiate into neurons once inside the central nervous system (CNS) is currently questioned. In this review, we collected exhaustive data on MSC/NCSC neural differentiation in vitro. We then analyzed pre-clinical cell therapy experiments in different models for neurological diseases and concluded that neural differentiation is probably not the leading property of adult MSC and NCSC concerning neurological pathologies management. Definitely, a fine analysis of the molecules that are secreted by MSC and NCSC would be of significant interest regarding their important contribution to the clinical and pathological recovery after CNS lesions. [less ▲]

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See detailNeural fate of Mesenchymal Stem Cells and Neural Crest Stem Cells : Which ways to get neurons for cell therapy purpose ?
Neirinckx, Virginie ULg; Coste, Cécile ULg; Rogister, Bernard ULg et al

in Trends in cell signaling pathways in neuronal fate decision (2013)

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See detailIn Vivo Tumorigenesis Was Observed after Injection of In Vitro Expanded Neural Crest Stem Cells Isolated from Adult Bone Marrow
Wislet, Sabine ULg; Poulet, Christophe ULg; Neirinckx, Virginie ULg et al

in PLoS ONE (2012), 7(10), 46425

Bone marrow stromal cells are adult multipotent cells that represent an attractive tool in cellular therapy strategies. Several studies have reported that in vitro passaging of mesenchymal stem cells ... [more ▼]

Bone marrow stromal cells are adult multipotent cells that represent an attractive tool in cellular therapy strategies. Several studies have reported that in vitro passaging of mesenchymal stem cells alters the functional and biological properties of those cells, leading to the accumulation of genetic aberrations. Recent studies described bone marrow stromal cells (BMSC) as mixed populations of cells including mesenchymal (MSC) and neural crest stem cells (NCSC). Here, we report the transformation of NCSC into tumorigenic cells, after in vitro long-term passaging. Indeed, the characterization of 6 neural crest-derived clones revealed the presence of one tumorigenic clone. Transcriptomic analyses of this clone highlighted, among others, numerous cell cycle checkpoint modifications and chromosome 11q down-regulation (suggesting a deletion of chromosome 11q) compared with the other clones. Moreover, unsupervised analysis such as a dendrogram generated after agglomerative hierarchical clustering comparing several transcriptomic data showed important similarities between the tumorigenic neural crest-derived clone and mammary tumor cell lines. Altogether, it appeared that NCSC isolated from adult bone marrow represents a potential danger for cellular therapy, and consequently, we recommend that phenotypic, functional and genetic assays should be performed on bone marrow mesenchymal and neural crest stem cells before in vivo use, to demonstrate whether their biological properties, after ex vivo expansion, remain suitable for clinical application. [less ▲]

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See detailMesenchymal stem cells and neural crest stem cells from adult bone marrow: characterization of their surprising similarities and differences.
Wislet, Sabine ULg; Laudet, Emerence ULg; Neirinckx, Virginie ULg et al

in Cellular and Molecular Life Sciences : CMLS (2012), 69(15), 2593-2608

The generation of neuronal cells from stem cells obtained from adult bone marrow is of significant clinical interest in order to design new cell therapy protocols for several neurological disorders. The ... [more ▼]

The generation of neuronal cells from stem cells obtained from adult bone marrow is of significant clinical interest in order to design new cell therapy protocols for several neurological disorders. The recent identification in adult bone marrow of stem cells derived from the neural crest stem cells (NCSC) might explain the neuronal phenotypic plasticity shown by bone marrow cells. However, little information is available about the nature of these cells compared to mesenchymal stem cells (MSC), including their similarities and differences. In this paper, using transcriptomic as well as proteomic technologies, we compared NCSC to MSC and stromal nestin-positive cells, all of them isolated from adult bone marrow. We demonstrated that the nestin-positive cell population, which was the first to be described as able to differentiate into functional neurons, was a mixed population of NCSC and MSC. More interestingly, we demonstrated that MSC shared with NCSC the same ability to truly differentiate into Tuj1-positive cells when co-cultivated with paraformaldehyde-fixed cerebellar granule neurons. Altogether, those results suggest that both NCSC and MSC can be considered as important tools for cellular therapies in order to replace neurons in various neurological diseases. [less ▲]

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See detailValidation of Adult Bone Marrow Stromal Cells in Cellular Therapy Protocols, using a Mouse Model for Parkinson’s Disease
Neirinckx, Virginie ULg; Laudet, Emerence; Rogister, Bernard ULg et al

Poster (2012, May 04)

Parkinson’s disease (PD) is the second most common neurodegenerative disorder in industrialized countries. Its main characteristic relies in a progressive loss of dopaminergic (DA) neurons in the ... [more ▼]

Parkinson’s disease (PD) is the second most common neurodegenerative disorder in industrialized countries. Its main characteristic relies in a progressive loss of dopaminergic (DA) neurons in the Substantia Nigra pars compacta (SNpc), resulting in a deficient dopamine release in the striatum and then promoting important defects in motility regulation. Unfortunately, motor symptoms are generally diagnosed once 80% of nigrostriatal neurons are already lost. The emergence of neuroprotective/-restorative strategies is then increasingly raising hope, and a lot of people now focus on cell therapy experiments. Adult bone marrow stromal stem cells (BMSCs) have already been demonstrated as ideal candidates for cell therapy in nervous lesions, regarding their high multipotency and the fact they can be easily harvested in the patient himself. After it has been demonstrated that some BMSCs arise from the embryonic neural crest (NC), we compared NC-BMSCs and mesenchymal (M)-BMSCs in vitro, in terms of differenciation abilities and more particularly in terms of neural fate. We then wanted to investigate and compare the potential usefulness of both populations in the context of a neurological pathology. We have validated a MPTP mouse model, mimicking the specific loss of nigral neurons, and started setting up a cell therapy experiment using stereotaxic brain injection of the two types of BMSCs. The survival rate of grafted cells was analyzed as well as their migration or differentiation, and their ability to restore neuronal loss was also observed. Our first results showed that once grafted inside the brain of MPTP mice, NC-BMSCs survive for about a week, staying tightly close to each other the injection track with no visible sign of migration. Afterwards, cells begin to disappear and we only observe a mean survival rate of 1% after 28 days. Looking at the nigrostriatal pathway integrity, neural crest-BMSCs don’t seem to induce any improvement: they don’t differenciate into neural cells, neither replace lost DA cells, and they do not induce any sprouting of surviving DA neurons. While the M-BMSCs graft experiment has to be completed, these first results showed that NC-BMSCs at the stem cell state are not able to restore the lesioned system, and maybe a pre-differenciation step would be required to trigger those cells into a neuronal fate before grafting them in a MPTP-mouse brain. [less ▲]

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See detailAdult neural crest stem cells and neuronal fate decision
Neirinckx, Virginie ULg; Rogister, Bernard ULg; Wislet, Sabine ULg

in Trends in cell signaling pathways in neuronal fate decision (2012)

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See detailAdult bone marrow: which stem cells for cellular therapy protocols in neurodegenerative disorders?
Wislet-Gendebien, Sabine ULg; Laudet, Emerence ULg; Neirinckx, Virginie ULg et al

in Journal of Biomedicine & Biotechnology (2011)

The generation of neuronal cells from stem cells obtained from adult bone marrow is of significant clinical interest in order to design new cell therapy protocols for several neurological disorders. The ... [more ▼]

The generation of neuronal cells from stem cells obtained from adult bone marrow is of significant clinical interest in order to design new cell therapy protocols for several neurological disorders. The recent identification in adult bone marrow of stem cells derived from the neural crests (NCSC) might explain the neuronal phenotypic plasticity shown by bone marrow cells. However, little information is available about the nature of these cells compared to mesenchymal stem cells (MSC). In this manuscript, we will review all information available concerning NCSC from adult tissues and their possible use in regenerative medecine. Moreover, as multiple recent studies showed the beneficial effect of bone marrow stromal cells in neurodegenerative diseases, we will discuss which stem cells isolated from adult bone marrow should be more suitable for cell replacement therapy. [less ▲]

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See detailNeural crest stem cells from adult bone marrow: a new source for cell replacement therapy?
Glejzer, Aneta; Neirinckx, Virginie ULg; Rogister, Bernard ULg et al

in Wislet, Sabine (Ed.) Advance in Regenerative Medicine (2011)

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