References of "Monfort, Mélanie"
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See detailUn allèle KEL*02mod responsable d'une exclusion apparente de maternité
MONFORT, Mélanie ULg; PEYRARD, Thierry; ARNAUD, Lionel et al

in Transfusion Clinique et Biologique (2013), 20

The patient’s rare KEL:1,-2 phenotype was highlighted in course of a routine preoperative erythrocyte typing. Unexpectedly, her two daughters presented a KEL:-1,2 phenotype what appeared first as an ... [more ▼]

The patient’s rare KEL:1,-2 phenotype was highlighted in course of a routine preoperative erythrocyte typing. Unexpectedly, her two daughters presented a KEL:-1,2 phenotype what appeared first as an apparent maternity exclusion. Flow cytometry, genotyping and adsorption-elution analyses were then performed for those 3 patients. KEL genotyping showed that the patient’s genotype was KEL*01/KEL*02 whereas that of her daughters was KEL*02/KEL*02. By using polyclonal anti-KEL2 reagent, weak amount of KEL2 was identified on the patient’s erythrocytes, a result which was confirmed by both flow cytometry and adsorption-elution assays, suggesting that patient’s phenotype was in fact KEL:1,2w. These results are in favour of a weak expressed KEL*02 allele (KEL*2mod) transmission coding for a KEL2 antigen detected in some technical conditions only. Those results allowed to explain the apparent maternity exclusion based on initial KEL phenotype. This study also seems to confirm the presence of a compensatory mechanism of the KELmod allele deficient expression in heterozygote patients. A KEL phenotype retrospective study of 80.000 subjects showed a local KEL:1,-2 frequency four times lower than that described in literature. Moreover, a significant number of those individuals would in reality be KEL:1,2w, what still would decrease the real frequency of the KEL:1,2 subjects. [less ▲]

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See detailMaladie hémolytique néonatale modérée due à un anti‐RH46
MONFORT, Mélanie ULg

Poster (2013, June)

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See detailMaladie hémolytique néonatale modérée
KEUTGENS, Aurore ULg; MONFORT, Mélanie ULg; WAGEMANS, Danielle et al

in Revue Médicale de Liège (2012), 67(7-8), 403-406

A Caucasian woman, with a A+ CCD.ee K neg erythrocyte phenotype and no history of blood transfusion, delivered a first child who developed mild anemia. The direct antiglobulin test performed on the ... [more ▼]

A Caucasian woman, with a A+ CCD.ee K neg erythrocyte phenotype and no history of blood transfusion, delivered a first child who developed mild anemia. The direct antiglobulin test performed on the newborn red blood cells belonging to the A+ CCD.ee K neg group, was strongly positive for IgG. During the pregnancy and after the delivery, the woman had a negative irregular antibody screening test, using standard red blood cells. However, at birth, using a collection of thawed red blood cells with rare phenotypes (private antigens), the lab showed an antibody anti-Wra in the maternal serum. The activity of the maternal antibody, with a titer of 16, was completely inhibited by dithiothreitol, indicating the nature IgM of the circulating antibody. The presence of the antigen Wra on the surface of the newborn and its biological father red blood cells was confirmed. The concentration of IgG anti-Wra on baby erythrocytes was demonstrated by the presence of the antibody anti-Wra in the eluate. This case illustrates the difficulties to detect antibodies against private antigens on baby erythrocytes, responsible of hemolytic diseases of newborn. Indeed, standard red blood cell panels used for irregular antibodies screening test do not express generally those private antigens. [less ▲]

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See detailUn allèle Kmod responsable d'une exclusion apparente de maternité
MONFORT, Mélanie ULg

Speech (2011)

Contexte.- Le bilan immuno-hématologique préopératoire d’une patiente l’avait identifiée du phénotype érythrocytaire rare KEL:1,-2. De manière inattendue, ses 2 filles convoquées pour un éventuel don ... [more ▼]

Contexte.- Le bilan immuno-hématologique préopératoire d’une patiente l’avait identifiée du phénotype érythrocytaire rare KEL:1,-2. De manière inattendue, ses 2 filles convoquées pour un éventuel don dirigé furent trouvées de phénotype KEL:-1,2, ce qui constituait une exclusion apparente de maternité. Méthodes.- Une exploration complémentaire du système KEL a été réalisée chez ces 3 personnes par génotypage, adsorption-élution et cytométrie en flux. Résultats.- Le génotypage KEL*1/2 a révélé que la patiente était de génotype KEL*1/KEL*2 tandis que ses 2 filles étaient KEL*2/KEL*2. Une très faible expression de l’antigène KEL2 a pu être mise en évidence sur les hématies de la patiente avec un réactif anti-KEL2 polyclonal (1+) et a été confirmée par adsorption-élution et cytométrie en flux ; le phénotype de la patiente est donc en réalité KEL:1,W2. Les analyses en cytométrie en flux ont par ailleurs montré que les hématies de la patiente présentaient une réactivité anti-KEL1 supérieure (+46%) à celle d’hématies témoins KEL:1,2 (n=3) et de façon similaire, que les hématies de ses 2 filles présentaient une réactivité anti-KEL2 supérieure (+33%) à celle d’hématies témoins KEL:1,2 (n=3). Conclusion.- Les résultats obtenus sont en faveur de la transmission par la patiente à ses 2 filles d’un allèle KEL*2 faiblement exprimé (KEL*2mod) codant pour un antigène KEL2 détectable uniquement sous certaines conditions techniques. Ceci permet d’expliquer l’exclusion apparente de maternité basée sur le phénotypage KEL initial. De plus, cette étude semble indiquer l’existence d’un mécanisme compensatoire de l’expression déficiente d’un allèle KELmod chez les individus hétérozygotes. [less ▲]

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See detailEffet de la deleucocytation des concentres erythrocytaires sur les reactions transfusionnelles.
Mukagatare, I.; MONFORT, Mélanie ULg; de Marchin, J. et al

in Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine (2010), 17(1), 14-9

SUBJECT: In order to assess the impact of leukocyte reduction, all transfusion reactions reported at Liege Teaching Hospital's Blood Bank 2 years before and after the implementation of universal leukocyte ... [more ▼]

SUBJECT: In order to assess the impact of leukocyte reduction, all transfusion reactions reported at Liege Teaching Hospital's Blood Bank 2 years before and after the implementation of universal leukocyte reduction of red blood cells concentrates which started-up on 1 January 2005 were evaluated. STUDY DESIGN AND METHODS: A retrospective analysis of transfusion reactions from 1 January 2003 to 31st December 2006 was undertaken. Data were collected from computerized reports, which were entered as soon as a transfusion reaction was reported. Symptoms were classified in different reaction's categories. Blood cultures, antibody screening and direct antiglobulin test were performed. Differences between the two time periods, before (2003-2004) and after (2005-2006) universal leukoreduction were determined by the Chi-square test and significance was defined as a p value less than 0.05. RESULTS: During period before the implementation of systematic leukoreduction, 68.7% of red blood cells transfused were leukoreduced. A total of 365 transfusion reactions in 91,996 red blood cells units transfused (0.4%) were reported, of which 266 were classified as febrile non-hemolytic transfusion reactions (72.9%), followed by allergic reactions (7.1%) and miscellaneous reactions (3.8%). When comparing the two-time periods, the rate of all transfusion reactions in general significantly decreased from 0.49 to 0.31% (p<0.001). Therefore, universal leukocyte reduction significantly reduced the rates of febrile non-hemolytic transfusion reactions (0.35% versus 0.24%; p=0.002) and allergic reactions (0.05% versus 0.01%; p<0.001). CONCLUSION: Universal leukocyte reduction significantly reduced the rate of transfusion reactions. [less ▲]

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See detailCalcium chez les patients hemodialyses : calcemie totale ou calcium ionise ? Le laboratoire doit-il systematiquement fournir au clinicien une valeur de calcemie totale corrigee obtenue par calcul ?
Monfort, Mélanie ULg; Delanaye, Pierre ULg; Chapelle, Jean-Paul ULg et al

in Annales de Biologie Clinique (2008), 66(5), 573-6

Ionized calcium is the only physiologically active form of calcium. Because of the variation of albumin, pH and haemoconcentration observed during haemodialysis session in patients with chronic renal ... [more ▼]

Ionized calcium is the only physiologically active form of calcium. Because of the variation of albumin, pH and haemoconcentration observed during haemodialysis session in patients with chronic renal failure, measure of total calcium does not reflect the real variation of ionized calcium. However, many formulae to correct total calcium by albumin have been proposed but none of them has been validated in dialysis patients. At present time, computing progress permit laboratory to systematically provide a value of corrected total calcium on protocols but is it really indicated? Our results showed that any of those formulae allows obtaining a value of total calcium that possesses a significant critical difference in relation to total calcium. Thus, correction formulae must be abandoned in aid of ionized calcium in haemodialysis patients. [less ▲]

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