Unique recurrence patterns of cervical intraepithelial neoplasia following excision of the squamo-columnar junction.
Herfs, Michael ; SOMJA, Joan ; et al
in International journal of cancer. Journal international du cancer (2015), 136
Recent studies have identified a putative cell of origin for cervical intraepithelial neoplasia (CIN) and cervical cancer at the squamo-columnar junction (SCJ) and suggest that these cells may not ... [more ▼]
Recent studies have identified a putative cell of origin for cervical intraepithelial neoplasia (CIN) and cervical cancer at the squamo-columnar junction (SCJ) and suggest that these cells may not regenerate following excision (LEEP). This study addressed the impact of SCJ excision on the temporal dynamics, histologic and viral (HPV) characteristics of recurrent CIN. One hundred thirty one consecutive patients treated by excision and attending follow-up visits were enrolled. We compared recurrent and initial CIN with attention to excision margins, timing of recurrence, CIN grade, HPV types, p16 immunophenotype, and SCJ immunophenotype. During the follow-up period (up to four years), sixteen (12.2%) recurrences were identified. Four (25%) were identified at the first follow-up visit, closely resembled the initial CIN 2/3 in grade and HPV type, and were typically SCJ marker positive [SCJ(+)], suggesting non-excised (residual) disease. Twelve (75%) manifested after the first postoperative visit and all were in the ectocervix or in mature metaplastic epithelium. All of the 12 delayed recurrences were classified as CIN 1 and were SCJ (-). Nine of 11 SCJ (-) recurrences (82%) followed regressed spontaneously. Taken together, these results show that new lesions developing from any HPV infection are delayed and occur within the ectocervix or metaplastic epithelium. This dramatically lower risk of CIN 2/3 following successful SCJ excision suggests that removal of the SCJ could be a critical variable in reducing the risk of subsequent CIN 2/3 and cervical cancer. (c) 2014 Wiley Periodicals, Inc. [less ▲]Detailed reference viewed: 48 (6 ULg)
EVALUATION OF THE PRACTICABILITY OF A RAPID INTRAPARTUM SCREENING BASED STRATEGY FOR PREVENTION OF GROUP B STREPTOCOCCUS PERINATAL INFECTIONS USING A REAL TIME PCR PERFORMED BY MIDWIVES AS A POINT OF CARE TEST.
MEEX, Cécile ; SACHELI, Rosalie ; DESCY, Julie et al
Poster (2014, November 12)Detailed reference viewed: 56 (19 ULg)
Parametrial boost does not replace optimal BT treatment in 102 patients with locally advanced cervical cancer (LACC)
; DE CUYPERE, Marjolein ; KRIDELKA, Frédéric et al
Conference (2014, September)Detailed reference viewed: 40 (2 ULg)
Tumor Angiogenesis and Lymphangiogenesis: Microenvironmental Soil for Tumor Progression and Metastatic Dissemination
Paupert, Jenny ; Van De Velde, Maureen ; Kridelka, Frédéric et al
in Feige, Jean-Jacques; Pagès, Gilles; Soncin, Fabrice (Eds.) Molecular Mechanisms of Angiogenesis (2014)Detailed reference viewed: 25 (8 ULg)
Persistent Right-Sided Umbilical Vein and Azygos Continuity associated with Ophtalmological and Pulmonary Malformations. Prenatal Diagnosis of an Exceptional Case
LEPAGE, Sylvie ; CAPELLE, Xavier ; EMONTS, Patrick et al
in Gynecology and Obstetrics (2014), 4(12),Detailed reference viewed: 17 (1 ULg)
La menace d'accouchement premature. Prise en charge dans un service universitaire de grossesses a risque : evaluation de la pratique clinique comparee aux recommandations internationals.
; Kridelka, Frédéric ; Nisolle, Michelle et al
in Revue medicale de Liege (2014), 69(12), 658-62
Premature birth poses a real problem of public health. As the principal cause of foetal ill-health and perinatal mortality, it generates high healthcare costs. By seeking to prevent early labour and to ... [more ▼]
Premature birth poses a real problem of public health. As the principal cause of foetal ill-health and perinatal mortality, it generates high healthcare costs. By seeking to prevent early labour and to deal with its causes, a good obstetrical practice can reduce its negative impact, both medical and financial, on society. This article describes the results of a study of threatened preterm delivery admissions at the Citadelle hospital in Liege during the year 2012. The findings are compared to international guidelines with a view to identify aspects that could be improved. [less ▲]Detailed reference viewed: 28 (3 ULg)
Improved computer-assisted analysis of the global lymphatic network in human cervical tissues.
Balsat, Cédric ; ; GOFFIN, Frédéric et al
in Modern Pathology : An Official Journal of the United States & Canadian Academy of Pathology, Inc (2014), 27(6), 887-98
Lymphatic dissemination is a key event in cervical cancer progression and related tumor lymphatic markers are viewed as promising prognostic factor of nodal extension. However, validating such parameters ... [more ▼]
Lymphatic dissemination is a key event in cervical cancer progression and related tumor lymphatic markers are viewed as promising prognostic factor of nodal extension. However, validating such parameters requires an objective characterization of the lymphatic vasculature. Here, we performed a global analysis of the lymphatic network using a new computerized method applied on whole uterine cervical digital images. Sixty-eight cases of cervical neoplasia (12 CIN3, 10 FIGO stage 1A and 46 stage IB1) and 10 cases of normal cervical tissue were reacted with antibodies raised against D2-40, D2-40/p16 and D2-40/Ki67. Immunostained structures were automatically detected on whole slides. The lymphatic vessel density (D2-40), proliferating lymphatic vessel density (D2-40/ki67) and spatial lymphatic distribution in respect to the adjacent epithelium were assessed from normal cervix to early cervical cancer and correlated with lymphovascular space invasion and lymph node status. Prominent lymphatic vessel density and proliferating lymphatic vessel density are detected under the transformation zone of benign cervix and no further increase is noted during cancer progression. Notably, a shift of lymphatic vessel distribution toward the neoplastic edges is detected. In IB1 cervical cancer, although intra- and peritumoral lymphatic vessel density are neither correlated with lymphovascular space invasion nor with lymph node metastasis, a specific spatial distribution with more lymphatic vessels in the vicinity of tumor edges is predictive of lymphatic dissemination. Herein, we provide a new computerized method suitable for an innovative detailed analysis of the lymphatic network. We show that the transformation zone of the benign cervix acts as a baseline lymphangiogenic niche before the initiation of neoplastic process. During cancer progression, this specific microenvironment is maintained with lymphatic vessels even in closer vicinity to tumor cells.Modern Pathology advance online publication, 6 December 2013; doi:10.1038/modpathol.2013.195. [less ▲]Detailed reference viewed: 62 (21 ULg)
Evaluation of CellSolutions BestPrep(R) Automated Thin-Layer Liquid-Based Cytology Papanicolaou Slide Preparation and BestCyte(R) Cell Sorter Imaging System.
; GOFFIN, Frédéric ; Kridelka, Frédéric et al
in Acta cytologica (2014)
Objective: A double-blind study was conducted to compare the performance of the new BestPrep(R) (CellSolutions) liquid-based thin-layer Papanicolaou (Pap) test with ThinPrep(R) (Hologic). Study Design ... [more ▼]
Objective: A double-blind study was conducted to compare the performance of the new BestPrep(R) (CellSolutions) liquid-based thin-layer Papanicolaou (Pap) test with ThinPrep(R) (Hologic). Study Design: Samples from the study patients (n = 105) were collected twice in the same encounter with the ThinPrep sample always taken first and the BestPrep sample collected second. Slides were prepared according to both manufacturers' protocols and evaluated using manual microscopic review and the BestCyte(R) cell sorter imaging system (CellSolutions). Diagnostic truth for each case was determined by independent manual review of both slides by multiple pathologists and histology when available. The presence of atypical squamous cells of undetermined significance was the threshold for positive for sensitivity and specificity calculations. Results: BestPrep and ThinPrep, by manual review, had sensitivities for high-grade squamous intraepithelial lesion (HSIL) cases of 100 and 95.6%, respectively. Using the BestCyte cell sorter, both had 100% sensitivity. For the same HSIL cases, the digene HC2 high-risk human papillomavirus DNA test had sensitivities of 100% (BestPrep) and 95.6% (ThinPrep). Specificities were 71.4% (BestPrep) and 54.8% (ThinPrep). Conclusions: BestPrep was equivalent to ThinPrep for manual review even though BestPrep was always the second sample collected. The BestCyte cell sorter provides a practical alternative to manual review for both BestPrep and ThinPrep slides. (c) 2014 S. Karger AG, Basel. [less ▲]Detailed reference viewed: 73 (6 ULg)
A phase 1b study of trebananib in combination with pegylated liposomal doxorubicin or topotecan in women with recurrent platinum-resistant or partially platinum-sensitive ovarian cancer.
; ; et al
in Gynecologic oncology (2014), 135(1), 25-33
OBJECTIVE: To examine the tolerability and antitumor activity of trebananib plus pegylated liposomal doxorubicin (PLD) or topotecan in recurrent platinum-resistant or partially platinum-sensitive ovarian ... [more ▼]
OBJECTIVE: To examine the tolerability and antitumor activity of trebananib plus pegylated liposomal doxorubicin (PLD) or topotecan in recurrent platinum-resistant or partially platinum-sensitive ovarian cancer. METHODS: In this open-label phase 1b study, patients received trebananib 10mg/kg or 15mg/kg IV QW plus PLD 50mg/m(2) (cohorts A1 and A3, respectively) or topotecan 4mg/m(2) (cohorts B1 and B3, respectively). Endpoints were dose-limiting toxicity (DLT; primary); treatment-emergent adverse events (AEs), overall response rate, anti-trebananib antibodies, and pharmacokinetics (secondary). RESULTS: 103 patients were enrolled. One patient in A1 and B1 had DLTs. Across all cohorts, the most common AEs were nausea, fatigue, and peripheral edema. Across both trebananib plus PLD cohorts (A1/A3), grade 4 AEs were pulmonary embolism, disease progression, and anemia. Two patients had grade 5 intestinal perforation (n=1) and sudden death (n=1). Across both trebananib plus topotecan cohorts (B1/B3), grade 4 AEs were neutropenia, hypokalemia, decreased granulocyte count, chest pain, dyspnea, decreased neutrophil count, and pulmonary embolism. Two patients had grade 5 disease progression. One patient had grade 5 pleural effusion associated with progressive disease. Confirmed objective response rates were 36.0% (A1), 34.8% (A3), 16.7% (B1), and 0.0% (B3). Median progression-free survival duration (months) was 7.4 (A1), 7.1 (A3), 3.5 (B1), and 3.1 (B3), respectively. No drug-drug interactions were apparent. CONCLUSIONS: Trebananib 10mg/kg and 15mg/kg IV QW plus PLD or topotecan appear to have acceptable toxicity profiles in recurrent platinum-resistant or partially platinum-sensitive ovarian cancer. Antitumor activity was evident across all cohorts. [less ▲]Detailed reference viewed: 24 (1 ULg)
A Clinical and Pathological Overview of Vulvar Condyloma Acuminatum, Intraepithelial Neoplasia and Squamous Cell Carcinoma
LEONARD, Boris ; Kridelka, Frédéric ; DELBECQUE, Katty et al
in BioMed Research International (2014)Detailed reference viewed: 51 (19 ULg)
Outcomes of women with endometrial cancer after introducing a robotics program
LAMBERT, Marie ; TIMMERMANS, Marie ; DELBECQUE, Katty et al
Poster (2013, September)Detailed reference viewed: 28 (10 ULg)
Belgian Register and Reference Centres for Gestational Trophoblastic Diseases
TIMMERMANS, Marie ; DELCOMINETTE, Sarah ; DELBECQUE, Katty et al
Poster (2013, September)Detailed reference viewed: 31 (11 ULg)
Atypical recurrence of a placental site trophoblastic tumor four years after hysterectomy for benign condition: Case report and review of literature.
RAUW, Laetitia ; DELBECQUE, Katty ; GOFFIN, Frédéric et al
in Gynecologic oncology case reports (2013), 6
*Rare case of PSTT limited to the vagina presenting eight years after last pregnancy and four years after hysterectomy*Differential diagnosis with other vaginal tumors can be challenging but it is ... [more ▼]
*Rare case of PSTT limited to the vagina presenting eight years after last pregnancy and four years after hysterectomy*Differential diagnosis with other vaginal tumors can be challenging but it is critical because behavior and management are different.*Stage-adapted management is proposed and surgery is the mainstay treatment for localized disease. [less ▲]Detailed reference viewed: 30 (2 ULg)
Assuétudes et grossesse: comment détruire un projet de naissance
Emonts, Patrick ; MASSON, Véronique ; CHANTRAINE, Frédéric et al
in Revue Médicale de Liège (2013), 68(5-6), 239-244
Les femmes enceintes sont conscientes que toute forme d’addiction durant leur grossesse peut être préjudiciable à leur enfant. Pourtant, de nombreuses gestantes continuent de fumer, de boire de l’alcool ... [more ▼]
Les femmes enceintes sont conscientes que toute forme d’addiction durant leur grossesse peut être préjudiciable à leur enfant. Pourtant, de nombreuses gestantes continuent de fumer, de boire de l’alcool, de consommer des drogues illicites ou d’absorber des médicaments, car ces dépendances sont particulièrement tenaces. Le quatuor de tête en termes de préjudice fœtal est composé du tabac, de l’alcool, de la cocaïne et de l’ecstasy. La période de grossesse est le meilleur moment pour mettre fin à ces addictions. Aussi, est-il indispensable de sensibiliser le grand public, les pouvoirs politiques ainsi que les médecins sur le fait que les assuétudes durant la grossesse représentent une inégalité de santé et d’espérance de vie importante pour l’enfant à naître. [less ▲]Detailed reference viewed: 159 (34 ULg)
Matrix metalloproteinase-2 governs lymphatic vessel formation as an interstitial collagenase.
Detry, Benoît ; Erpicum, Charlotte ; Paupert, Jenny et al
in Blood (2012), 119(21), 5048-56
Lymphatic dysfunctions are associated with several human diseases, including lymphedema and metastatic spread of cancer. Although it is well recognized that lymphatic capillaries attach directly to ... [more ▼]
Lymphatic dysfunctions are associated with several human diseases, including lymphedema and metastatic spread of cancer. Although it is well recognized that lymphatic capillaries attach directly to interstitial matrix mainly composed of fibrillar type I collagen, the interactions occurring between lymphatics and their surrounding matrix have been overlooked. In this study, we demonstrate how matrix metalloproteinase (MMP)–2 drives lymphatic morphogenesis through Mmp2-gene ablation in mice, mmp2 knockdown in zebrafish and in 3D-culture systems, and through MMP2 inhibition. In all models used in vivo (3 murine models and thoracic duct development in zebrafish) and in vitro (lymphatic ring and spheroid assays), MMP2 blockage or down-regulation leads to reduced lymphangiogenesis or altered vessel branching. Our data show that lymphatic endothelial cell (LEC) migration through collagen fibers is affected by physical matrix constraints (matrix composition, density and cross-linking). Transmission electron microscopy (TEM) and confocal reflection microscopy using DQ-collagen highlight the contribution of MMP2 to mesenchymal-like migration of LEC associated with collagen fiber remodeling. Our findings provide new mechanistic insight into how LEC negotiate an interstitial type I collagen barrier and reveal an unexpected MMP2-driven collagenolytic pathway for lymphatic vessel formation and morphogenesis. [less ▲]Detailed reference viewed: 172 (70 ULg)
Registre belge et centre de référence des maladies trophoblastiques gestationnelles
DELCOMINETTE, Sarah ; TIMMERMANS, Marie ; DELBECQUE, Katty et al
Poster (2012)Detailed reference viewed: 26 (1 ULg)
Le cas clinique du mois: Radiotherapie dans le cadre d'une maladie de Paget touchant la region genitale.
; ; et al
in Revue medicale de Liege (2012), 67(2), 61-3
Urogenital Paget disease is usually treated by surgery. However, in case of recurrence or if multilating surgery is foreseen, radiotherapy seems to open a reasonable alternative. We report a successful ... [more ▼]
Urogenital Paget disease is usually treated by surgery. However, in case of recurrence or if multilating surgery is foreseen, radiotherapy seems to open a reasonable alternative. We report a successful treatment with radiotherapy in a patient with urogenital Paget's disease. [less ▲]Detailed reference viewed: 12 (3 ULg)
Surgical outcomes of women with endometrial cancer after introducing a robotic program
LAMBERT, Marie ; DELBECQUE, Katty ; DE CUYPERE, Marjolein et al
Poster (2012)Detailed reference viewed: 21 (13 ULg)
Aberrant promoter methylation and expression of UTF1 during cervical carcinogenesis.
; Mouallif, Mustapha ; et al
in PLoS ONE (2012), 7(8), 42704
Promoter methylation profiles are proposed as potential prognosis and/or diagnosis biomarkers in cervical cancer. Up to now, little is known about the promoter methylation profile and expression pattern ... [more ▼]
Promoter methylation profiles are proposed as potential prognosis and/or diagnosis biomarkers in cervical cancer. Up to now, little is known about the promoter methylation profile and expression pattern of stem cell (SC) markers during tumor development. In this study, we were interested to identify SC genes methylation profiles during cervical carcinogenesis. A genome-wide promoter methylation screening revealed a strong hypermethylation of Undifferentiated cell Transcription Factor 1 (UTF1) promoter in cervical cancer in comparison with normal ectocervix. By direct bisulfite pyrosequencing of DNA isolated from liquid-based cytological samples, we showed that UTF1 promoter methylation increases with lesion severity, the highest level of methylation being found in carcinoma. This hypermethylation was associated with increased UTF1 mRNA and protein expression. By using quantitative RT-PCR and Western Blot, we showed that both UTF1 mRNA and protein are present in epithelial cancer cell lines, even in the absence of its two main described regulators Oct4A and Sox2. Moreover, by immunofluorescence, we confirmed the nuclear localisation of UTF1 in cell lines. Surprisingly, direct bisulfite pyrosequencing revealed that the inhibition of DNA methyltransferase by 5-aza-2'-deoxycytidine was associated with decreased UTF1 gene methylation and expression in two cervical cancer cell lines of the four tested. These findings strongly suggest that UTF1 promoter methylation profile might be a useful biomarker for cervical cancer diagnosis and raise the questions of its role during epithelial carcinogenesis and of the mechanisms regulating its expression. [less ▲]Detailed reference viewed: 16 (5 ULg)
Les cancers viro-induits: interrelations genetique-environnement
Delvenne, Philippe ; ; et al
in Revue Médicale de Liège (2012), 67(5-6), 381-9
Among cancers diagnosed worldwide on a yearly basis, 20% are thought to be associated with a viral infection. The viruses involved are, by order of decreasing incidence, the hepatitis viruses, the ... [more ▼]
Among cancers diagnosed worldwide on a yearly basis, 20% are thought to be associated with a viral infection. The viruses involved are, by order of decreasing incidence, the hepatitis viruses, the papillomaviruses and the Epstein-Barr virus. These virus-induced cancers generate a high level of interest not only for the study of mechanisms involved in the neoplastic transformation, but also for the set-up of specific immunotherapies including prophylactic and therapeutic antitumor vaccination. [less ▲]Detailed reference viewed: 16 (2 ULg)