References of "Jacquet, Nicolas"
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See detailLes aspects stratégiques du bioraffinage de seconde génération en Région Wallonne : symbiose industrielle ou autonomie complète ?
Jacquet, Nicolas ULg; Istasse, Thibaut ULg; Berchem, Thomas ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (in press)

Introduction. Le bioraffinage s’impose progressivement, à l’échelle commerciale, comme complément ou alternative aux filières de production traditionnelles. Littérature. A ce jour, les principaux sites ... [more ▼]

Introduction. Le bioraffinage s’impose progressivement, à l’échelle commerciale, comme complément ou alternative aux filières de production traditionnelles. Littérature. A ce jour, les principaux sites industriels de bioraffinage s’orientent vers la production de biocarburants (bioéthanol ou biodiésel). Le territoire wallon, de par son accès limité aux ressources végétales renouvelables, semble cependant s’orienter spécifiquement et compétitivement vers la production à plus faibles tonnages de bioproduits. Conclusions. Afin de mener à bien cette initiative, cette étude propose d’argumenter le meilleur choix stratégique devant être ciblé par la Wallonie: développer de nouveaux sites de production autonomes (énergétiquement suffisants et avec des filières d’approvisionnement indépendantes) ou s’ancrer à des systèmes ou des installations de production existants (et bénéficier des facilités déjà opérationnelles d’accès à l’énergie et aux matières premières). Le design de nouvelles unités de bioraffinage intégrées à des unités de production existantes semble être la meilleure option à définir pour le territoire wallon. [less ▲]

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See detailScalable temperature induced stress for the large-scale production of functionalized Bifidobacteria
Nguyen, Huu Thanh ULg; Razafindralambo, Hary; Richel, Aurore ULg et al

in Journal of Industrial Microbiology & Biotechnology (in press)

The application of sub-lethal stresses is known to be an efficient strategy to enhance survival of probiotic bacteria during drying processes. In this context, we previously showed that the application of ... [more ▼]

The application of sub-lethal stresses is known to be an efficient strategy to enhance survival of probiotic bacteria during drying processes. In this context, we previously showed that the application of heat stress upon the entry into stationary phase increased significantly the viability of Bifidobacterium bifidum. However, this heat shock has been considered only in small scale bioreactor and no information is available for a possible scaling-up strategy. Five different operating scales (0.2 L, 2 L, 20 L, 200 L and 2000 L) have thus been tested and the results showed that the viability of B. bifidum increases from 3.15 to 6.57 folds, depending on the scale considered. Our observations pointed out the fact that the heat stress procedure is scalable according to the main outcome, i.e. increases in cell viability, but other factors have to be taken into account. Among these factors, dissolved carbon dioxide seems to play a significant role since it explain the differences observed between the test performed at lab-scale and in industrial conditions. [less ▲]

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See detailLa légistation REACH: une opportunité d’innovation pour l’économie biobasée
Schmetz, Quentin ULg; Istasse, Thibaut ULg; Jacquet, Nicolas ULg et al

Scientific conference (2016, May 12)

Cet exposé fait le point sur les tendances en cours dans ce secteur au travers de divers cas concrets tels que la substitution de retardateur de flamme, plastifiant et surfactant par des alternatives ... [more ▼]

Cet exposé fait le point sur les tendances en cours dans ce secteur au travers de divers cas concrets tels que la substitution de retardateur de flamme, plastifiant et surfactant par des alternatives issues du végétale. Il aborde également les différents programmes de recherche réalisés au laboratoire de Chimie Biologique Industrielle de Gembloux Agro-Bio Tech qui ont permis le développement de nouvelles substances biobasées en accord avec une chimie plus durable et répondant aux exigences de REACH. [less ▲]

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See detailPRODUCTION OF BIOFUELS AND BIOBASED COMPOUNDS IN URBAN BIOREFINERIES: A STRATEGY TO MANAGE WASTE IN WALLONIA?
Richel, Aurore ULg; Haubruge, Eric ULg; Jacquet, Nicolas ULg

Conference (2016, May)

“Urban biorefining” is an original concept aiming at using urban wastes (household wastes, municipal wastes, industrial liquid and/or solid residues and side-products, etc.), mainly of vegetal origin, for ... [more ▼]

“Urban biorefining” is an original concept aiming at using urban wastes (household wastes, municipal wastes, industrial liquid and/or solid residues and side-products, etc.), mainly of vegetal origin, for the production of an array of biofuels and bioproducts. This “urban biorefining” concept fits particularly with the economic, geographic and politic contexts and constraints of the Walloon Region (south part of Belgium). Indeed, Walloon Region is a very small territory (area of about 6,504 sq mi) with a temperate climate. Supply feedstock, mainly arising form forestry and agriculture, are thus rather restricted, submitted to importation, and subjected to non-standardized quality. Several examples of our regional strategy, still available on an industrial scale, are herein proposed and detailed. [less ▲]

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See detailNovel butanol pretreatment significantly improves delignification and saccharification of different lignocellulosic biomasses
Schmetz, Quentin ULg; Teramura, Hiroshi; Jacquet, Nicolas ULg et al

Conference (2016, April 05)

Organosolv pretreatment using diluted acid and butanol allows the separation of cellulose, hemicelluloses and lignin into three distinct phases. The butanol process has been investigated on six different ... [more ▼]

Organosolv pretreatment using diluted acid and butanol allows the separation of cellulose, hemicelluloses and lignin into three distinct phases. The butanol process has been investigated on six different biomasses: tall fescue, sugarbeet pulp, sugarcane bagasse, beech wood, eucalyptus and Japanese cedar. Dilute acid pretreatment has been performed under similar conditions for comparison. [less ▲]

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See detailLa Chimie Biologique Industrielle à Gembloux Agro-Bio Tech
Berchem, Thomas ULg; Istasse, Thibaut ULg; Schmetz, Quentin ULg et al

Conference given outside the academic context (2016)

Présentation du laboratoire de Chimie Biologique Industrielle et illustration de nos principales activités; point sur les recherches de trois jeunes doctorants. Leur travail consiste en la valorisation de ... [more ▼]

Présentation du laboratoire de Chimie Biologique Industrielle et illustration de nos principales activités; point sur les recherches de trois jeunes doctorants. Leur travail consiste en la valorisation de matrices biologiques (déchets agricoles, industriels et forestiers,...) pour produire une gamme de nouveaux produits, biocarburants ou molécules chimiques. [less ▲]

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See detailLa législation REACH: une opportunité d’innovation pour l’économie biobasée
Schmetz, Quentin ULg; Richel, Aurore ULg; Jacquet, Nicolas ULg et al

Conference (2016, January 20)

REACH is the acronym given to the integrated legislative system for the recording, evaluation, authorization and restriction of chemicals introduced by the European Union. The aim of REACH is to improve ... [more ▼]

REACH is the acronym given to the integrated legislative system for the recording, evaluation, authorization and restriction of chemicals introduced by the European Union. The aim of REACH is to improve and ensure health and environmental protection while boosting competitiveness and innovation in the European chemicals industry. This system thus strongly encourages the transition to a biobased economy by encouraging the development and the operation of production patterns and new substitutes greener for the environment and health. Biotechnological production pathways, including the use of renewable raw materials have thus been developed over the past decade in the industry, and this trend is confirmed for the coming years. This presentation provides an update on current trends in this sector through various concrete cases such as flame retardant, plasticizer and surfactant substituted by alternatives from the vegetal. It also discusses the various research programs carried out in the Laboratory of Biological and Industrial Chemistry of Gembloux Agro-Bio Tech that allowed the development of new biobased substances consistent with a more sustainable chemistry and meeting REACH requirements. [less ▲]

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See detailProduction de nanocellulose à partir de pâte à papier: Hydrolyse enzymatique et valorisation des coproduits dans une stratégie de bioraffinage forestier intégré
Bombeck, Pierre-Louis ULg; Richel, Aurore ULg; Jacquet, Nicolas ULg et al

Conference (2016, January 19)

Présentation succincte de la thématique de recherche doctorale et de son contexte

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See detailEtude du potentiel des sous-produits vinicoles pour des applications à haute valeur ajoutée
Berchem, Thomas ULg; Jacquet, Nicolas ULg; Richel, Aurore ULg

Conference (2016, January 19)

Grape growing is one of the largest crops around world. Grape pomaces seem to be an interesting source of bio-based molecules but, despite this potential, these byproducts are untapped. Their uses are ... [more ▼]

Grape growing is one of the largest crops around world. Grape pomaces seem to be an interesting source of bio-based molecules but, despite this potential, these byproducts are untapped. Their uses are limited to poor value added applications like composting, agricultural spreading or bioethanol. This work focuses on high value added valorisation possibilities of grape pomace. It takes part in the current trend of using natural bioactive molecules in pharmaceutical, cosmetic or food industry, through the extraction of polyphenols. Firstly, chemical characterization is performed on grape pomace from two grape varieties (Cabernet sauvignon and Pinot noir) in order to determine variability. Secondly, the same approach is applied to grape seeds and skins. Finally, a method of extraction of polyphenols is developed. Results shown in this work tend to indicate a high valorisation potential of grape pomace thank to the identification of compounds like bioactive molecules and especially polyphenols (0.7% in seeds and 0.4% in skins) as well as high concentrations of other compounds in seeds (lignin (43-53%), grape oil (12%), proteins (12%)) and in skins (lignin (20%), tartaric acid (4%), proteins (10%)) of which the valorisation potential from wine-making byproducts needs to be explored. [less ▲]

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See detailMonitoring the laccase reaction of vanillin and poplar hydrolysate
Sòti, Valentin; Jacquet, Nicolas ULg; Apers, Sandra et al

in Journal of Chemical Technology & Biotechnology (2016), 91(6), 1914-1922

BACKGROUND: Laccase is an intensively researched enzyme for industrial use. Except for decolorisation measurements, HPLC analysis is the conventional method for monitoring the phenolic removal during ... [more ▼]

BACKGROUND: Laccase is an intensively researched enzyme for industrial use. Except for decolorisation measurements, HPLC analysis is the conventional method for monitoring the phenolic removal during laccase enzyme reaction. This paper reports an investigation of the continuous UV absorbance follow-up of the laccase reaction with steam pretreated poplar hydrolysate. RESULTS: Vanillin was used as a model substrate and lignocellulose xylose rich fraction (XRF) as a biologically complex substrate for laccase detoxification. The reaction was followed by HPLC-UV as well as by UV spectrometric measurements. Results suggest that the reaction can be successfully monitored by measuring the change of UV absorbance at 280 nm, without previous compound separation. In case of XRF experiments the spectrophotometric follow-up is especially useful, as HPLC analysis takes a long time and provides less information than in case of single substrates. The method seems to be suitable for optimization and process control. CONCLUSION: The obtained results can help to construct a fast, easy and straightforward monitoring system for laccase-phenolic substrate reactions. [less ▲]

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See detailCharacterization of saccharides released during an in vitro pepsin-pancreatin digestion of corn flour using HPAEC-PAD
Odjo, Djosse Psijus Sylvanus ULg; Bera, François ULg; Jacquet, Nicolas ULg et al

in Stärke = Starch (2016), 68

Saccharides released after an in vitro pepsin-pancreatin digestion of corn flour were quantified and characterized using high-performance anion-exchange chromatography coupled with a pulsed amperometric ... [more ▼]

Saccharides released after an in vitro pepsin-pancreatin digestion of corn flour were quantified and characterized using high-performance anion-exchange chromatography coupled with a pulsed amperometric detector (HPAEC-PAD) and two colorimetric approaches (glucose oxidase/ peroxidase assay and the 3,5-dinitrosalicylic acid assay). HPAEC-PAD revealed five major saccharides as the result of in vitro pepsin-pancreatin digestion of corn grain: glucose, isomaltose, maltose, maltotriose, and glucosyl-maltotriose. Concentrations of glucose released as measured by the three methods assessed are similar when the in vitro pepsin-pancreatin digestion is followed by post incubation with amyloglucosidase. This post incubation unfortunately leads to a loss of information about the degree of polymerization of oligosaccharides that can impact their absorption through intestinal tract. HPAEC-PAD gives both qualitative and quantitative information and then seems more suitable for a nutritional appreciation of the digestion of starchy feedstuffs. [less ▲]

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See detailMetabolic profile of mixed culture acidogenic fermentation of lignocellulosic residues and the effect of upstream substrate fractionation by steam explosion
Perimenis, Anastasios; van Aarle, Ingrid; Nicolay, Thomas et al

in Biomass Conversion and Biorefinery (2016), 6

Lignocellulosic biomass residues have attracted attention for the sustainable production of molecules for material and energetic use through biochemical conversion. Their recalcitrant structure prevents a ... [more ▼]

Lignocellulosic biomass residues have attracted attention for the sustainable production of molecules for material and energetic use through biochemical conversion. Their recalcitrant structure prevents a broader use and asks for the development of sustainable techniques that can efficiently separate, recover and valorize the constituting components. In a cascading concept, residual streams of such processes can be further exploited in an attempt to valorize the largest possible fraction of the initial material. Three lignocellulosic substrates, namely dried sugar beet pulp, wheat bran and miscanthus straw, were upstream fractionated by steam explosion to extract the hemicellulose fraction. This study evaluated the valorization of the residual solid fraction through mixed acidogenic fermentation for the production of volatile fatty acids (VFA) as platform chemicals. Batch experiments have been conducted for the reference material (non-treated) and the solid fraction remaining after steam explosion, with and without the addition of an external mixed inoculum. Steam explosion residues contained less hemicellulose than the initial materials. The difference in the fermentation profile between steam explosion residues and non-treated substrates is dependent on the substrate. Maximum total VFA (tVFA) concentration was 18.8 gCOD/kgmixed_liquor, and maximum yield of chemical oxygen demand (COD) conversion into tVFAwas 33 % for the case of non-treated inoculated beet pulp. [less ▲]

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See detailSteam Explosion Process
Jacquet, Nicolas ULg; Haubruge, Eric ULg; Richel, Aurore ULg

Scientific conference (2015, December 17)

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See detailIndustrial Biological Chemistry laboratory activities
Jacquet, Nicolas ULg; Richel, Aurore ULg

Conference (2015, November 26)

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See detailIndustrial Biological Chemistry laboratory activities (GxABT-CBI)
Jacquet, Nicolas ULg

Conference (2015, November 26)

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See detailLa chimie biologique industrielle à Gembloux Agro-Bio Tech : Un aperçu de nos activités
Berchem, Thomas ULg; Istasse, Thibaut ULg; Schmetz, Quentin ULg et al

Conference given outside the academic context (2015)

Présentation du laboratoire de Chimie Biologique Industrielle et illustration de nos principales activités; point sur les recherches de trois jeunes doctorants. Leur travail consiste en la valorisation de ... [more ▼]

Présentation du laboratoire de Chimie Biologique Industrielle et illustration de nos principales activités; point sur les recherches de trois jeunes doctorants. Leur travail consiste en la valorisation de matrice biologique (déchets agricoles, industriels et forestiers,...) pour produire une gamme de nouveaux produits, biocarburants ou molécules chimiques. [less ▲]

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See detailEt si nos déchets devenaient la source de nouveaux produits. Notion d'économie circulaire
Richel, Aurore ULg; Jacquet, Nicolas ULg; Berchem, Thomas ULg et al

Conference given outside the academic context (2015)

Description et illustration de nos activités visant à utiliser les déchets agricoles et forestiers, les déchets végétaux et industriels et nos déchets ménagers pour produire une gamme de nouveaux produits ... [more ▼]

Description et illustration de nos activités visant à utiliser les déchets agricoles et forestiers, les déchets végétaux et industriels et nos déchets ménagers pour produire une gamme de nouveaux produits, biocarburants ou molécules chimiques. Cette présentation se consacre aux nouveaux produits et polymères générés au départ de ces déchets dans une approche d'économie circulaire et de priorité des usages. [less ▲]

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See detailComparative biochemical analysis after steam pretreatment of lignocellulosic agricultural waste biomass from Williams Cavendish banana plant (Triploid Musa AAA group)
Kamdem, Irenée ULg; Jacquet, Nicolas ULg; Tiappi Deumaga, Mathias Florian ULg et al

in Waste Management & Research : The Journal of the International Solid Wastes & Public Cleansing Association (2015)

The accessibility of fermentable substrates to enzymes is a limiting factor for the efficient bioconversion of agricultural wastes in the context of sustainable development. This paper presents the ... [more ▼]

The accessibility of fermentable substrates to enzymes is a limiting factor for the efficient bioconversion of agricultural wastes in the context of sustainable development. This paper presents the results of a biochemical analysis performed on Williams Cavendish Lignocellulosic Biomass (WCLB) after steam cracking (SC) and steam explosion (SE) pretreatments. Solid (S) and liquid (L) fractions (Fs) obtained from SC pretreatment performed at 180°C (SLFSC180) and 210°C (SLFSC210) generated, after diluted acid hydrolysis, the highest proportions of neutral sugar (NS) contents, specifically 52.82±3.51 and 49.78±1.39 %w/w WCLB’s dry matter (DM), respectively. The highest proportions of glucose were found in SFSC210 (53.56±1.33 %w/w DM) and SFSC180 (44.47±0.00 %w/w DM), while the lowest was found in unpretreated WCLB (22.70±0.71 %w/w DM). Total NS content assessed in each LF immediately after SC and SE pretreatments was less than 2 %w/w of the LF’s DM, thus revealing minor acid autohydrolysis consequently leading to minor NS production during the steam pretreatment. WCLB subjected to SC at 210°C (SC210) generated up to 2.7-fold bioaccessible glucan and xylan. SC and SE pretreatments showed potential for the deconstruction of WCLB (delignification, depolymerisation, decrystallization and deacetylation), enhancing its enzymatic hydrolysis. The concentrations of enzymatic inhibitors such as 2-furfuraldehyde and 5-(hydroxymethyl)furfural from LFSC210 were the highest (41 and 21 µg mL-1, respectively). This study shows that steam pretreatments in general and SC210 in particular are required for efficient bioconversion of WCLB. Yet, biotransformation through biochemical processes (e.g., anaerobic digestion) must be performed to assess the efficiency of these pretreatments. [less ▲]

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See detailEt si nos déchets devenaient la source de nouveaux produits. Notion d'économie circulaire
Jacquet, Nicolas ULg; Istasse, Thibaut ULg; Berchem, Thomas ULg et al

Conference given outside the academic context (2015)

Notre objectif et nos recherches se concentrent sur l'utilisation des déchets agricoles et forestiers, les déchets végétaux et industriels et nos déchets ménagers pour produire une gamme de nouveaux ... [more ▼]

Notre objectif et nos recherches se concentrent sur l'utilisation des déchets agricoles et forestiers, les déchets végétaux et industriels et nos déchets ménagers pour produire une gamme de nouveaux produits, biocarburants ou molécules chimiques. [less ▲]

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