References of "Hayette, Marie-Pierre"
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See detailAntifungigrammes: méthodologies et interprétation
Hayette, Marie-Pierre ULg

Conference (2017, June 15)

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See detailExemples tirés de la pratique: les phanères
Hayette, Marie-Pierre ULg

Conference (2017, June 08)

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See detailMolecular surveillance of Plasmodium falciparum resistance to artemisinin-based combination therapies in the Democratic Republic of Congo
Mvumbi Makaba, Dieudonné; Bobanga Lengu, Thierry; Kayembe Ntumba, Jean-Marie et al

in PLoS ONE (2017)

Malaria is a major public health problem in the Democratic Republic of Congo. Despite progress achieved over the past decade in the fight against malaria, further efforts have to be done such as in the ... [more ▼]

Malaria is a major public health problem in the Democratic Republic of Congo. Despite progress achieved over the past decade in the fight against malaria, further efforts have to be done such as in the surveillance and the containment of Plasmodium falciparum resistant strains. We investigated resistance to artemisinin-based combination therapies currently in use in Democratic Republic of Congo by surveying molecular polymorphisms in three genes: pfcrt, pfmdr1 and pfk13 to explore possible emergence of amodiaquine, lumefantrine or artemisinin resistance in Democratic Republic of Congo. This study essentially revealed that resistance to chloroquine is still decreasing while polymorphism related to amodiaquine resistance seems to be not present in Democratic Republic of Congo, that three samples, located in the east of the country, harbor Pfmdr1 amplification and that none of the mutations found in South-East Asia correlated with artemisinine resistance have been found in Democratic Republic of Congo. But new mutations have been identified, especially the M476K, occurred in the same position that the M476I previously identified in the F32-ART strain, strongly resistant to artemisinine. Antimalarial first-line treatments currently in use in Democratic Republic of Congo are not associated with emergence of molecular markers of resistance. [less ▲]

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See detailHigh concordance of Pfdhfr and Pfdhps genotypes between matched peripheral and placental isolates of delivered women in Bobo-Dioulasso, Burkina Faso
Cissé, Mamoudou; Awandare, Gordon; Some, Fabrice et al

in Annals of Parasitology (2017), 63(2), 111-116

Whether maternal peripheral parasites constitute a representative sample of the overall population infecting the individual, remains unknown in Burkina Faso. We therefore compared Pfdhfr and Pfdhps ... [more ▼]

Whether maternal peripheral parasites constitute a representative sample of the overall population infecting the individual, remains unknown in Burkina Faso. We therefore compared Pfdhfr and Pfdhps génotypes between matched peripheral and placental isolates. PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of polymorphic codons of the Pfdhfr gene (51, 59, 108 and 164) and the Pfdhps gene (437 and 540) was performed in 18 matched peripheral and placental dried blood spots of delivered women in Bobo-Dioulasso. Both Pfdhfr and Pfdhps genes were successfully genotyped in 94.4% (17/18) of the matched samples. Only 8.8% (3/34) of génotypes were of the wild type, while 20.6% (7/34), 20.6% (7/34), 23.5% (8/34) and 26.5% (9/34) comprised one, two, three and four mutations, respectively. None of the samples carried both Pfdhfr I164L and Pfdhps K540E mutations. A concordance of 82.4% was observed in matched samples for both the Pfdhfr and Pfdhps genes. Setting placental allèles as the reference, a concordance of 100% was obtained with Pfdhfr mutation S108N, Pfdhfr mutation C59R+S108N, and Pfdhfr mutation N51I+C59R +S108N, respectively. Likewise, a concordance of 85.7% was observed with the Pfdhps mutation A437G. For epidemiological purposes, peripheral blood Pfdhfr and Pfdhps genotyping is sufficient for monitoring SP resistant molecular markers in pregnant women. [less ▲]

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See detailRecent uptake of intermittent preventive treatment during pregnancy with sulfadoxine-pyrimethamine is associated with increased prevalence of Pfdhfr mutations in Bobo-Dioulasso, Burkina Faso
Cisse, Mamoudou; Awandare, Gordon; Soulama, Alamissa et al

in Malaria Journal (2017), 16

Background: The impact of sulfadoxine–pyrimethamine (SP) used as intermittent preventive treatment during pregnancy (IPTp-SP) on mutant parasite selection has been poorly documented in Burkina Faso. This ... [more ▼]

Background: The impact of sulfadoxine–pyrimethamine (SP) used as intermittent preventive treatment during pregnancy (IPTp-SP) on mutant parasite selection has been poorly documented in Burkina Faso. This study sought first to explore the relationship between IPTp-SP and the presence of mutant parasites. Second, to assess the Relationship between the mutant parasites and adverse pregnancy outcomes. Methods: From September to December 2010, dried blood spots (DBS) were collected during antenatal care visits and at delivery from 109 pregnant women with microscopically confirmed falciparum malaria infection. DBS were analysed by PCR–restriction fragment length polymorphism (PCR–RFLP) for the polymorphisms at codons 51, 59, 108 and 164 of the Pfdhfr gene and codons 437 and 540 in the Pfdhps gene. Results: Both the Pfdhfr and Pfdhps genes were successfully genotyped in 92.7% (101/109) of the samples. The prevalence of Pfdhfr mutations N51I, C59R and S108N was 71.3, 42.6 and 64.4%, respectively. Overall, 80.2% (81/101) of samples carried the Pfdhps A437G mutation. None of the samples had the Pfdhfr I164L and the Pfdhps K540E mutations. The prevalence of the triple mutation N51I + C59R + S108N was 25.7% (26/101). The use of IPTp-SP was associated with a threefold increased odds of Pfdhfr C59R mutation [crude OR 3.29; 95% CI (1.44–7.50)]. Pregnant women with recent uptake of IPTp-SP were at higher odds of both the Pfdhfr C59R mutation [adjusted OR 4.26; 95% CI (1.64–11.07)] and the Pfdhfr intermediate-to-high resistance, i.e., ≥ 2 Pfdhfr mutations [adjusted OR 3.45; 95% CI (1.18–10.07)]. There was no statistically significant association between the presence of the Pfdhfr intermediate-tohigh resistance and parasite densities or both maternal haemoglobin level and anaemia. Conclusion: The data indicate that despite the possibility that IPTp-SP contributes to the selection of resistant parasites, it did not potentiate pregnancy-associated malaria morbidity, suggesting the continuation of SP use as IPTp in Burkina Faso. [less ▲]

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See detailLe cas clinique du mois. Actinomycose pelvienne pseudo-tumorale : il fallait y penser
KAKKOS, Athanasios ULg; GONNE, Elodie ULg; COIMBRA MARQUES, Carla ULg et al

in Revue Médicale de Liège (2017), 72(1), 10-13

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See detailEpidemiology and reporting of candidaemia in Belgium
Trouvé, Charlotte; Blot, Stijn; HAYETTE, Marie-Pierre ULg et al

in European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology (2016)

The primary aim of this study was to collect national epidemiological data on candidaemia and to determine the reporting time of species identification and antifungal susceptibility in clinical practice ... [more ▼]

The primary aim of this study was to collect national epidemiological data on candidaemia and to determine the reporting time of species identification and antifungal susceptibility in clinical practice. During a 1-year period (March 2013 until February 2014), every first Candida isolate from each episode of candidaemia was included prospectively from 30 Belgian hospitals. Identification and susceptibility testing were performed according to local procedures and isolates were sent to the National Reference Center for Mycosis. Species identification was checked by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and internal transcribed spacer (ITS) sequencing in case no reliable identification was obtained by MALDI-TOF MS. Antifungal susceptibility testing was performed according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) methodology. A total of 355 isolates were retrieved from 338 patients. The mean incidence rate of candidaemia was 0.44 (range: 0.07 to 1.43) per 1000 admissions or 0.65 (range: 0.11 to 2.00) per 10,000 patient days. Candida albicans was most frequently found (50.4 %), followed by C. glabrata (27.3 %) and C. parapsilosis sensu lato (9.8 %). The overall resistance to fluconazole was 7.6 %, ranging from 3.9 % in C. albicans to 20.0 % in C. tropicalis. Only one C. glabrata isolate was resistant to the echinocandins. Four days after blood culture positivity, 99.7 % of the identifications and 90.3 % of the antifungal profiles were reported to the treating clinician. Candidaemia incidence rates differed up to 20-fold among Belgian hospitals; no clear factors explaining this difference were identified. The overall antifungal resistance rates were low but high azole resistance rates were recorded in C. tropicalis. [less ▲]

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See detailApplication of rep-PCR as molecular typing method for dermatophytes
SACHELI, Rosalie ULg; ADJETEY BAHUN, Akolé ULg; DARFOUF, Rajae ULg et al

Conference (2016, October 29)

Background The distinction of dermatophytes strains by conventional methods is problematic due to a lack of reliable characteristics between isolates. The ability to identify individual organisms allows ... [more ▼]

Background The distinction of dermatophytes strains by conventional methods is problematic due to a lack of reliable characteristics between isolates. The ability to identify individual organisms allows identification of infection sources, such as communal swimming and public washing rooms and apply appropriate control measures. Several outbreaks of dermatophytes infection have already been described in Europe and the feasibility of comparing DNA fingerprints is of interest in this particular field. We have successfully applied the rep-PCR method for typing dermatophytes species such as Microsporum audouinii, Trichophyton violaceum and Trichophyton mentagrophytes complex (T.mentagrophytes complex). Methods The Diversilab® system (bioMérieux, France) has been used to perform rep-PCR. Strains of M. audouinii (116) and T. violaceum (23) were collected during a national survey conducted by the National Reference center (NRC) for mycoses (Liège) in Belgium from February 2013 to March 2014. Strains of T. mentagrophytes complex (32) are part of the national collection of the NRC collected between 2012 and 2015. Results The Diversilab® is able to distinguish different subspecies between the same species complex as it was confirmed with the T. mentagrophytes complex. For this group of strains, two major groups are clearly visible after Diversilab analysis and correspond to white strains (ITS sequenced as T. interdigitale) and yellow strains (ITS sequenced as A. benhamiae) of T. mentagrophytes complex. Both groups share only 70% of similarity. Within a same species of dermatophyte, the Rep-PCR is also able to define several subgroups sometimes linked with epidemiological factors. Inside the T. violaceum strains circulating in Belgium, two different DNA fingerprints have been found. These two subgroups were well separated regarding the geographical location in Belgium (Wallonia and Flanders). Inside the M. audouinii species, 6 different DNA fingerprints have been detected with a main group. The degree of similarity between these six groups is comprised between 85,6% and 94%. Strains belonging to the six groups were defined as M. audouinii by ITS sequencing. Conclusion The DiversiLab® system with the Rep-PCR technology proved to be an efficient method for dermatophytes molecular typing. It is highly sensitive to discriminate small genotypic variation between two strains within the same species which is not possible with other molecular methods such as ITS sequencing. Inside the T. mentagrophytes complex, it permits to easily distinguish the yellow zoophilic strains from the white anthropophilic strain, hardly possible by microscopy. [less ▲]

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See detailUnusual Species of Dermatophytes: Rarely Identified or New?
HAYETTE, Marie-Pierre ULg; SACHELI, Rosalie ULg

in Mycopathologia (2016)

Dermatophytes are causing superficial mycosis in animals and humans. Depending on the geophilic, zoophilic or anthropophilic origin of the fungus but also on the immunological status of the patient ... [more ▼]

Dermatophytes are causing superficial mycosis in animals and humans. Depending on the geophilic, zoophilic or anthropophilic origin of the fungus but also on the immunological status of the patient, symptomatology can widely differ. Nevertheless, each species is currently associated with typical clinical manifestations, even if atypical localizations and/or clinical pictures are sometimes also reported. Diagnostic tools applied to species identification have been changing since the last two decades with the more frequent use of molecular methods currently considered nowadays as reference methods for species identification. It becomes obvious that the algorithm used for the distinction of closely related species needs to combine phenotypic and genomic methods. All these different points are discussed, and the most recent novel species causing or involved in human dermatophytosis are reported. [less ▲]

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See detailGenotypic characterization of T. mentagrophytes complex strains circulating in Belgium with the Diversilab® system.
SACHELI, Rosalie ULg; Utri, Tania ULg; ADJETEY BAHUN, Akolé ULg et al

Poster (2016, April 09)

Background Infections due to the zoophilic dermatophyte Arthroderma benhamiae are being more frequently diagnosed in Belgium since a few years. The most common source of infection is guinea pigs. This ... [more ▼]

Background Infections due to the zoophilic dermatophyte Arthroderma benhamiae are being more frequently diagnosed in Belgium since a few years. The most common source of infection is guinea pigs. This species which is referred to the Trichophyton species of A.benhamiae can cause tinea capitis, tinea corporis, tinea manus and more frequently tinea faciei. These strains appear with a bright yellow thallus in culture and do not easily sporulate making them difficult to identify. Sequencing of these strains reveals “Arthroderma benhamiae”. At the contrary white strains of T. mentagrophytes complex are identified by sequencing as “T. mentagrophytes variety interdigitale” or T. interdigitale. The aim of the study is the evaluation of the genetic heterogeneity of these two subtypes of the T. mentagrophytes complex by using the DiversiLab® system. Material and methods 32 strains were collected by the National Reference Center for mycoses between 2012 and 2015. The fungal strains were identified by microscopy or ITS sequencing. All strains appearing white with a yellow pigment in culture and being identified as “A. benhamiae” by DNA sequencing were included as well reference strains. Some selected strains appearing white in culture and being identified as “T. mentagrophytes var. interdigitale” by DNA sequencing were also included. The genotypic analysis was performed by the DiversiLab® system (bioMérieux) by DNA fingerprinting generation. Results Both groups showed huge differences in DNA fingerprints. The similarity calculated by the DiversiLab® tool between the two groups was 70%. This reflects a high genotypic heterogeneity regarding the two types of strains analyzed. This is surprising given that both groups belong to the same species complex. The comparison of these two distinct DNA fingerprints with the mold database of bioMérieux generated identification as “T. mentagrophytes” for both groups. We noticed that the library contained two distinct patterns of DNA fingerprints (profile 1, strains MK55-60 and profile 2, strains MK138-143). Yellow strains were highly similar to profile 1 and white strains were highly similar to profile 2. Unfortunately, no additional information regarding the subspecies implicated could be obtained from the manufacturer. However, this study demonstrates that it may represent respectively “yellow strains types Trichophyton species of A. benhamiae” and “ white strains types T. interdigitale”. Conclusion This study highlights the genotypic differences between two types of strains belonging to the T. mentagrophytes species complex. Yellow strains which incidence is increasing in Belgium are clearly different from other strains previously characterized and the DiversiLab® method shows a high efficiency for discriminating between these two species difficult to separate by microscopy. [less ▲]

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See detailEtude de dermatophytes isolés dans les services de revalidation et physiothérapie d’une structure hospitalière en région Liégeoise (Belgique)
SACHELI, Rosalie ULg; Utri, Tania ULg; Abdel-Sadick, Hidjab Abdoulaye ULg et al

Poster (2016, March 23)

Objectifs : Les dermatophytes sont responsables d’infections de la peau, des ongles et du cuir chevelu. Les espèces anthropophiles peuvent se transmettre d’une personne à une autre par un simple contact ... [more ▼]

Objectifs : Les dermatophytes sont responsables d’infections de la peau, des ongles et du cuir chevelu. Les espèces anthropophiles peuvent se transmettre d’une personne à une autre par un simple contact. Leur présence en milieu sportif (salles de sport, piscine, tatamis), douches collectives, vestiaires des écoles, a été largement décrite. Par contre il existe peu de littérature concernant les établissements de soins. C’est pourquoi, nous nous sommes intéressés au risque potentiel d’infection par des dermatophytes en milieu hospitalier et particulièrement dans les salles de kinésithérapie et de revalidation ainsi que dans une piscine du CHU de Liège. Matériel et méthodes : Trois sites du CHU de Liège ont été investigués: le site du Sart Tilman (ST), le site Ourthe Amblève (OA) et le site Notre-Dame des Bruyères (NDB). Les prélèvements ont été effectués en salle de revalidation et kinésithérapie ainsi qu’à la piscine de revalidation. Ces prélèvements ont été réalisés sur les surfaces à l’aide de géloses de contact (milieu de Sabouraud/chloramphénicol/actidione/gentamicine, Tritium Microbiologie, Pays-bas). L’identification des espèces fongiques a été réalisée par observation macro-microscopique des cultures, spectrométrie de masse (Maldi-Tof) et séquençage moléculaire. Résultats : Au total, 552 prélèvements ont été réalisés parmi lesquels 15 dermatophytes ont été recensés (2,72%). On retrouve: 7 T. rubrum, 7 T. interdigitale, 1 T. mentagrophytes var. mentagrophytes. Sur le site ST, 207 prélèvements ont été effectués parmi lesquels 4 T. rubrum, 1 T. interdigitale et 1 T. mentagrophytes var. mentagrophytes (zoophile). Sur le site OA, parmi les 226 prélèvements effectués, 4 Trichophyton interdigitale et 3 Trichophyton rubrum ont été retrouvés. Aucun dermatophyte n’a été isolé de l’eau de piscine ou pédiluve. Sur le site de NDB, sur 119 prélèvements, seuls 2 isolats de Trichophyton interdigitale ont été identifiés. Les prélèvements positifs pour les dermatophytes concernent des tapis de gymnastique, vélos, rameurs, balances, trampolines, demi-sphères d’équilibre, table de kinésithérapie, sols de douches et vestiaires de la piscine. Conclusion Grâce à ces investigations, nous avons pu mettre en évidence sur le matériel de revalidation et les sols des vestiaires, la présence de deux espèces anthropophiles, T. rubrum et T. interdigitale fréquemment impliquées dans les cas d’onychomycoses et atteintes cutanées. Une souche zoophile de T. mentagrophytes a également été mise en évidence. Cette étude démontre qu’un renforcement de la fréquence de nettoyage des sols et des instruments utilisés dans les salles de sport du CHU de Liège est nécessaire de façon à minimaliser les risques de contamination de patients et du personnel médical. [less ▲]

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See detailLouse-borne relapsing fever in a refugee from Somalia arriving in Belgium
DARCIS, Gilles ULg; Hayette, Marie-Pierre ULg; BONTEMS, Sébastien ULg et al

in Journal of Travel Medicine : Official Publication of the International Society of Travel Medicine & the Asia Pacific Travel Health Association (2016), 23(3),

We report a case of louse-borne relapsing fever (LBRF) in a refugee from Somalia who had arrived in Belgium a few days earlier. He complained of myalgia and secondarily presented fever. Blood smears ... [more ▼]

We report a case of louse-borne relapsing fever (LBRF) in a refugee from Somalia who had arrived in Belgium a few days earlier. He complained of myalgia and secondarily presented fever. Blood smears revealed spirochetes later identified as Borrelia recurrentis. LBRF should be considered in countries hosting refugees, particularly those who transit through endemic regions. [less ▲]

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See detailA One-year Survey of Microsporum audouinii infections in Belgium: epidemiological and genotypic characterisation
SACHELI, Rosalie ULg; ADJETEY BAHUN, Akolé ULg; DARFOUF, Rajae ULg et al

in Clinical Microbiology and Infection (2016), 22(3), 2859-28517

During recent years the proportion of tinea capitis (TC) infections due to Microsporum audouinii (M. audouinii) has increased both in Belgium and other European countries. To better understand the ... [more ▼]

During recent years the proportion of tinea capitis (TC) infections due to Microsporum audouinii (M. audouinii) has increased both in Belgium and other European countries. To better understand the emergence of this species in the country, the Belgian National Reference Center (NRC) for dermatophytes launched an epidemiological survey on the main anthropophilic dermatophytes causing TC in Belgium and including the genomic characterization of M. audouinii isolates. In total, 116 strains of M. audouinii were confirmed and characterized by the DiversiLab® system (bioMérieux). Six genotypic variants were identified, among which one major group included 90 isolates and the reference strain. Another variant group (11 strains) was exclusively confined to a geographical region in South Belgium. Analysis of epidemiological characteristics of the infected population showed that the main age category concerned 5-9 year-old children with a sex ratio (Male/Female) of 1.97. Data concerning the geographic origin of the family revealed a majority of Belgian nationality (44.7%), suggesting that the contamination has originated in Belgium. Other nationalities primarily originated from Africa. At this time, no clear correlation has been established between one particular strain and a specific country of origin. [less ▲]

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See detailHigh Prevalence of Plasmodium falciparum Infection in Asymptomatic Individuals from the Democratic Republic of the Congo
Mvumbi, Dieudonné; Bobanga, Thierry; Melin, Pierrette ULg et al

in Malaria Research and Treatment (2016), 2016

Malaria remains a major public health problem in the Democratic Republic of Congo (DRC) with 14 million cases reported by the WHOMalaria Report in 2014. Asymptomaticmalaria cases are known to be prevalent ... [more ▼]

Malaria remains a major public health problem in the Democratic Republic of Congo (DRC) with 14 million cases reported by the WHOMalaria Report in 2014. Asymptomaticmalaria cases are known to be prevalent in endemic areas and are generally untreated,resulting in a significant source of gametocytes that may serve as reservoir of disease transmission. Considering that microscopycertainly underestimates the prevalence of Plasmodium infections within symptomatic carriers and that PCR assays are currentlyrecognized as the most sensitive methods for Plasmodium identification, this study was conducted to weigh the asymptomatic carriage in DRC by a molecular method. Six provinces were randomly selected for blood collection in which 80 to 100 individuals were included in the study. Five hundred and eighty blood sampleswere collected andmolecular diagnosiswas performed. Globally, almost half of the samples collected from asymptomatic individuals (280/580; 48.2%) had Plasmodium infections and the most species identified was P. falciparum alone in combination with P. malariae. The high prevalence reported here should interpellate the bodies involved in malaria control in DR Congo to take into account asymptomatic carriers in actions taken and consider asymptomatic malaria as a major hurdle for malaria elimination. [less ▲]

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See detailRapport du Centre National de Référence Mycoses Liège 2015
SACHELI, Rosalie ULg; HAYETTE, Marie-Pierre ULg

Book published by ISP-WIV (2016)

Au cours de l’année 2015, tous les aspects des missions attribuées au CNR ont été couverts, comme l’identification d’isolats de levures et champignons filamenteux, l’aide au diagnostic de mycoses rares ... [more ▼]

Au cours de l’année 2015, tous les aspects des missions attribuées au CNR ont été couverts, comme l’identification d’isolats de levures et champignons filamenteux, l’aide au diagnostic de mycoses rares, la détermination de la sensibilité aux antifongiques et l’amélioration de techniques de typage et d’identification moléculaire. Le CNR de Liège se focalise principalement sur l’identification des mycoses superficielles isolées de phanères. Parmi les techniques d’identification des levures, l’identification par spectrométrie de masse (Maldi-tof) est l’outil n°1 qui est utilisé. Les résultats sont confirmés par séquençage moléculaire si nécessaire. Parmi les outils moléculaires, une approche polyphasique est utilisée. La région ciblée en premier lieu dans le cas de l’identification d’une espèce est tout ou partie de la région ITS1-5,8S-ITS2 de l’ARN ribosomique (ARNr). Deux cibles complémentaires sont disponibles à savoir la région D1-D2 de la partie LSU (28S) de l’ARNr et la bêta-tubuline. Ces autres cibles sont utilisées en cas de confirmation de l’identification d’une espèce rare ou en cas de réponse non satisfaisante après une première amplification. Le CNR Mycose a acquis l’appareil Diversilab (BioMérieux). Cet appareil permet une caractérisation précise du génome des champignons filamenteux (y compris les dermatophytes) et des levures. Il a déjà été utilisé pour la caractérisation de souches issues d’une étude nationale. Il pourrait aussi être utilisé en cas d’épidémies notamment afin de caractériser rapidement le génome de la souche circulante. [less ▲]

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See detailInvestigation on possible transmission of monkeys' Plasmodium to human in a populations living in the equatorial rainforest of the Democratic republic of Congo
MVUMBI, Dieudonné; BOBANGA, Thierry; UMESUMBU, Solange et al

in International Journal for Parasitology: parasites and wild life (2016), 5

Plasmodiums are protozoa that may infect various hosts. Only five species are now recognized as naturally parasitizing humans: Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, Plasmodium ... [more ▼]

Plasmodiums are protozoa that may infect various hosts. Only five species are now recognized as naturally parasitizing humans: Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, Plasmodium ovale and Plasmodium knowlesi. This fifth species, P. knowlesi, previously identified as naturally parasitizing the monkey Macaca fascicularis, has been microscopically confused for a long time with P. malariae or P. falciparum and it was not possible to correctly differentiate them until the advent of molecular biology. To date, natural human infections with P. knowlesi only occur in Southeast Asia and a similar phenomenon of natural transmission of simian plasmodium to humans has not been reported elsewhere. This study was conducted to investigate a possible transmission of African small monkey's plasmodium to humans in populations living near the rainforest of the Democratic Republic of Congo (DRC) where several species of non-human primates are living. Two successive real-time PCRs were identified in the literature and used in combination for purpose. Only P. falciparum was found in this study. However, studies with larger samples and with more advanced techniques should be conducted. [less ▲]

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See detailNew technologies in Mycology: PCR for diagnosis
Hayette, Marie-Pierre ULg

Conference (2015, November 19)

Molecular biology techniques are widely used in mycology. First of all they are used for confirmation of difficult or uncertain identifications. In this case PCR followed by ITS sequencing is generally ... [more ▼]

Molecular biology techniques are widely used in mycology. First of all they are used for confirmation of difficult or uncertain identifications. In this case PCR followed by ITS sequencing is generally used. For diagnostic purposes, PCR is mostly applied for the detection of invasive infections such as pneumocystosis, candidosis or aspergillosis. However these techniques (Candida and Aspergillus detection) are still not included in the recommandations given by EORTC by lack of standardisation. However, the development of new commercially available techniques, will probably change the diagnostic algorithm. New commercial PCR kits applied to the detection of dermatophytes are also recently available to the laboratories and they will be a good complement to culture-based diagnostic methods. [less ▲]

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See detailFungal skin infections: from the clinic to the laboratory
Hayette, Marie-Pierre ULg

Conference (2015, November 14)

Superficial fungal infections are largely represented in the world population and are mainly caused by dermatophytes. Clinical signs can be confusing and it is important to get a rapid diagnostic to apply ... [more ▼]

Superficial fungal infections are largely represented in the world population and are mainly caused by dermatophytes. Clinical signs can be confusing and it is important to get a rapid diagnostic to apply the right treatment.The diagnostic procedures are still culture-based methods but molecular technologies are recently available under commercial kits and will be probable part of a new algorithm in the laboratory diagnosis. [less ▲]

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