References of "Gustin, Pascal"
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See detailAssessment of a computed tomography guided injection technique of the lumbo-sacral disc in sheep.
Neveu, Fabienne ULg; Vandeweerd, Jean-Michel; Kirschvink, Nathalie et al

in Veterinary and Comparative Orthopaedics and Traumatology (2016), 29(2), 136-141

OBJECTIVES: Recent data indicate that degeneration of intervertebral discs occurs naturally in sheep, with a higher prevalence at the level of the lumbo-sacral disc. The objective of this ex vivo study ... [more ▼]

OBJECTIVES: Recent data indicate that degeneration of intervertebral discs occurs naturally in sheep, with a higher prevalence at the level of the lumbo-sacral disc. The objective of this ex vivo study was to evaluate a computed tomography (CT) guided method of injection into the ovine lumbo-sacral disc. METHODS: Six euthanatized sheep were used for identification of the approach plane, the optimal direction of the needle and the mean distance from skin to disc. Dissection after injection of coloured ink was used to determine the anatomical structures that were penetrated. In seven other animals, all spines were assessed beforehand by CT and magnetic resonance imaging to determine whether disc pathology was present. The final position of the needle was assessed by CT to determine the accuracy of the technique. Contrast agent was injected to identify any problems associated with administration of liquid into the disc. RESULTS: The CT guided injection technique was easy to perform and enabled adequate positioning of the needle into all (n = 7) lumbo-sacral discs. Distance between the skin and the disc ranged between 12 and 17 cm. No organ, vascular or nervous structure was penetrated and the needle path remained intramuscular without penetration of the peritoneal cavity. Contrast medium leaked out through three degenerate discs. CLINICAL SIGNIFICANCE: The current study described a consistently safe and accurate CT guided injection technique to the lumbo-sacral disc for future in vivo experimental studies that will use sheep as animal model for human intervertebral disc disease disease. [less ▲]

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See detailResidues in beeswax: a health risk for the consumer of honey and beeswax ?
Wilmart, O; Legrève, A; Scippo, Marie-Louise ULg et al

in Journal of Agricultural and Food Chemistry (2016), 64(44), 84258434

A scenario analysis in regard to the risk of chronic exposure of consumers to residues through the consumption of contaminated honey and beeswax was conducted. Twenty-two plant protection products and ... [more ▼]

A scenario analysis in regard to the risk of chronic exposure of consumers to residues through the consumption of contaminated honey and beeswax was conducted. Twenty-two plant protection products and veterinary substances of which residues have already been detected in beeswax in Europe were selected. The potential chronic exposure was assessed by applying a worst-case scenario based on the addition of a “maximum” daily intake through the consumption of honey and beeswax to the theoretical maximum daily intake through other foodstuffs. For each residue, the total exposure was finally compared to the acceptable daily intake. It is concluded that the food consumption of honey and beeswax contaminated with these residues considered separately does not compromise the consumer’s health, provided proposed action limits are met. In regard to residues of flumethrin in honey and in beeswax, “zero tolerance” should be applied. [less ▲]

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See detailContribution de la pédagogie inversée et du recours aux boitiers de votes à l’optimisation de l’antibiothérapie en médecine vétérinaire
Gustin, Pascal ULg; Vandeweerd, Jean-Michel; Detroz, Pascal ULg et al

in Detroz, Pascal; Borsu, Olivier (Eds.) actes du 27ème colloque de l ADMEE Europe : l'évaluation à la lumière des contextes et des disciplines (2015, January 28)

Une nouvelle intervention pédagogique a été mise en place en 2012-2013 en premier master. Basée sur les principes de la pédagogie inversée et de l’usage des boîtiers de votes, son objectif était ... [more ▼]

Une nouvelle intervention pédagogique a été mise en place en 2012-2013 en premier master. Basée sur les principes de la pédagogie inversée et de l’usage des boîtiers de votes, son objectif était d’améliorer la capacité des étudiants à réaliser un choix raisonné des médicaments antimicrobiens en situations cliniques simples en favorisant l’interactivité et la résolution de problèmes. Le dispositif et l’évaluation Trois heures de cours ont été consacrées à expliquer la démarche décisionnelle visant à optimaliser l’antibiothérapie et à présenter de manière magistrale une première famille d’antibiotiques. Des notes de cours détaillées et des résumés des points essentiels ont été mis à disposition des étudiants qui disposaient d’un boîtier vote électronique. Pour chaque situation, les questions majeures nécessaires à la structuration d’une démarche décisionnelle cohérente ont été sélectionnées par l’enseignant sur base des critères de choix des antibiotiques. Chacune d’elle était décomposée en sous-question pour lesquelles plusieurs solutions décisionnelles ont été proposées aux étudiants (Turning Point). A chaque étape, un vote suivi d’une discussion avec les étudiants était organisé, visant à comprendre le choix proposé par l’enseignant, discuter les choix alternatifs justifiés par les étudiants et à expliquer les erreurs décisionnelles mises en évidence par le vote. Une enquête de satisfaction a été réalisée. A l’examen oral de fin d’année, les connaissances et la capacité à structurer un schéma décisionnel ont été évaluées. Résultats et conclusion Le haut degré de satisfaction des étudiants, le sentiment d’un meilleur apprentissage de la démarche et la supériorité des notes d’examen des étudiants ayant suivi cette formation sont des éléments concordants en faveur d’un effet positif de l’intervention pédagogique sur la capacité décisionnelle des étudiants. Ce résultat a été obtenu par la combinaison de la pédagogie inversée, couplée à l’usage des boîtiers de vote et à la conception de questionnaires structurés pour discuter les démarches décisionnelles de manière interactive au sein d’une cohorte importante d’étudiants. [less ▲]

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See detailLes nouveaux outils de diagnostic et de pronostic de la myopathie atypique
Habyarimana, Jean Belt Adélite ULg; BOEMER, François ULg; Amory, Hélène ULg et al

in Proceeding de la 41ème Journée de la Recherche équine (2015)

In equines, ingestion of hypoglycin A, a toxin produced in the seeds (samaras) of sycamore maple (Acer pseudoplatanus) tree alters the energetic metabolism of muscle cells and results in atypical myopathy ... [more ▼]

In equines, ingestion of hypoglycin A, a toxin produced in the seeds (samaras) of sycamore maple (Acer pseudoplatanus) tree alters the energetic metabolism of muscle cells and results in atypical myopathy (AM). This alterations leads to a characteristic biochemical profile of acylcarnitines (AC) that enables to confirm the diagnosis of AM. This study aims at validating a methodology for the dosage of hypoglycin A in vegetal extracts but also in blood. In addition, the biochemical profile in AC has been determined in AM cases (5 survivors and 13 deceased) and in 5 horses suffering from exercise-induced myopathy. The AC profiles of these horses have been compared to the one of healthy horses (n = 35). This study showed that hypoglycin A was present in seeds and spring seedlings of sycamore and also in blood of AM cases horses. In addition, the establishment of AC profile contributes to the diagnostic and helps to assess the prognosis of AM cases. [less ▲]

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See detailEffect of corticosteroids on articular cartilage: have animal studies said everything?
Vandeweerd, Jean-Michel; Zhao, Yang; Nisolle, Jean-Francois et al

in Fundamental & Clinical Pharmacology (2015), 29(5), 427-438

Intra-articular (IA) corticosteroids (CS) have been used in the treatment of osteoarthritis for many years, although their effects on articular cartilage are not fully understood. To identify whether ... [more ▼]

Intra-articular (IA) corticosteroids (CS) have been used in the treatment of osteoarthritis for many years, although their effects on articular cartilage are not fully understood. To identify whether previous animal studies have provided enough evidence about the effects of CS, we undertook a systematic review that identified 35 relevant in vivo animal experimental studies between 1965 and 2014 assessing the effects of CS on either normal cartilage, or in either induced osteoarthritis (OA) or synovitis. The quality of the methodology was assessed. Deleterious effects, both structural and biochemical, have mainly been reported in rabbits and are associated with frequent administration of CS, sometimes at high dose and with systemic side effects. In dogs, four identified studies concluded that there were beneficial effects with methylprednisolone acetate (MPA) and triamcinolone hexacetonide therapy. In horses, MPA was mostly deleterious, while triamcinolone acetonide had positive effects in one study highly rated at quality assessment. However, many methodological weaknesses have been identified, such as the lack of pharmacokinetic and pharmocodynamics data and the large variation in doses between studies, the limited selection criteria at baseline, the absence of blinding, and the lack of statistics or appropriate controls for testing the effects of the vehicle of the drug. Those methodological weaknesses weaken the conclusions of numerous studies that assess beneficial or deleterious effects of CS on articular cartilage. Animal studies have not yet provided definitive data, and further research is required into the role of CS in articular pathobiology. [less ▲]

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See detailPotentiated interaction between ineffective doses of budesonide and formoterol to control the inhaled cadmium-induced up-regulation of metalloproteinases and acute pulmonary inflammation in rats.
Zhang, Wenhui; Zhi, Jianming; Cui, Yongyao et al

in PloS one (2014), 9(10), 109136

The anti-inflammatory properties of glucocorticoids are well known but their protective effects exerted with a low potency against heavy metals-induced pulmonary inflammation remain unclear. In this study ... [more ▼]

The anti-inflammatory properties of glucocorticoids are well known but their protective effects exerted with a low potency against heavy metals-induced pulmonary inflammation remain unclear. In this study, a model of acute pulmonary inflammation induced by a single inhalation of cadmium in male Sprague-Dawley rats was used to investigate whether formoterol can improve the anti-inflammatory effects of budesonide. The cadmium-related inflammatory responses, including matrix metalloproteinase-9 (MMP-9) activity, were evaluated. Compared to the values obtained in rats exposed to cadmium, pretreatment of inhaled budesonide (0.5 mg/15 ml) elicited a significant decrease in total cell and neutrophil counts in bronchoalveolar lavage fluid (BALF) associated with a significant reduction of MMP-9 activity which was highly correlated with the number of inflammatory cells in BALF. Additionally, cadmium-induced lung injuries characterized by inflammatory cell infiltration within alveoli and the interstitium were attenuated by the pre-treatment of budesonide. Though the low concentration of budesonide (0.25 mg/15 ml) exerted a very limited inhibitory effects in the present rat model, its combination with an inefficient concentration of formoterol (0.5 mg/30 ml) showed an enhanced inhibitory effect on neutrophil and total cell counts as well as on the histological lung injuries associated with a potentiation of inhibition on the MMP-9 activity. In conclusion, high concentration of budesonide alone could partially protect the lungs against cadmium exposure induced-acute neutrophilic pulmonary inflammation via the inhibition of MMP-9 activity. The combination with formoterol could enhance the protective effects of both drugs, suggesting a new therapeutic strategy for the treatment of heavy metals-induced lung diseases. [less ▲]

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See detailPerception des référentiels de compétences en médecine vétérinaire
Cambier, Carole ULg; Vandeweerd, JM; Perrenoud, P et al

in Proceedings du Congrès de l’Association Internationale de Pédagogie Universitaire (AIPU) (2014)

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See detailWhat does multiplication of tidemarks mean in the ovine femora-tibial joint ?
Pirson, R; Matagne, A; Nisolle, JF et al

in Osteoporosis International (2014), 25(S2), 172

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See detailBiochemical composition of cartilage with naturally occurring defects in the ovine femora-tibial joint
Matagne, A; Clegg, P; Tew, S et al

in Osteoporosis International (2014), 25(S2), 173

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See detailPrevalence of naturally occuring cartilage defects in the ovine knee
Vandeweerd, Jean-Michel; Hontoir, Fanny; Kirschvink, Nathalie et al

in Osteoarthritis and cartilage (2013), 21

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See detailA comparison of in vitro relaxant responses to ipratropium bromide, beta-adrenoceptor agonists and theophylline in feline bronchial smooth muscle.
Leemans, Jerome; Kirschvink, Nathalie; Gustin, Pascal ULg

in Veterinary journal (London, England : 1997) (2012), 193(1), 228-233

This study compares the potency and efficacy of different relaxant drugs including anticholinergic, beta-adrenergic and methylxanthine agents on acetylcholine-contracted feline bronchi, and investigates ... [more ▼]

This study compares the potency and efficacy of different relaxant drugs including anticholinergic, beta-adrenergic and methylxanthine agents on acetylcholine-contracted feline bronchi, and investigates the influence of the initial muscarinic-induced tone on bronchodilator response. Feline bronchi were removed from euthanased client-owned cats and were contracted with acetylcholine to cause either 40% or 80% of the acetylcholine maximal contraction. The efficacy and potency of bronchodilating drugs were obtained from cumulative dose-response curves with efficacy (E(max)) as the maximal relaxant response and potency (-logEC(50)) as the logarithm of the concentration of drug inducing 50% of maximal relaxation. Under low contractile tone (40%), all bronchodilators relaxed feline bronchi in a concentration-dependent manner with the following rank order of potency: formoterol>ipratropium bromide>fenoterol>isoprenaline>salbutamol>/=salmeterol>theophylline. E(max) values ranged from 80% to 100% depending on the tested drug. Constriction of feline bronchi with high-dose acetylcholine (80%) caused a rightward and downward shift of the beta(2)-mimetic dose-response curves. Significant decreases in -logEC(50) and E(max) values were reported for salbutamol, formoterol and salmeterol. This study provides evidence that existing classes of bronchodilators produce effective relaxation of acetylcholine-contracted feline bronchi and that airway responsiveness to beta(2)-stimulants is dependent on the magnitude of the initial muscarinic-induced tone. The clinical relevance of these in vitro findings has yet to be explored in clinical trials. [less ▲]

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See detailPrevalence of naturally occurring cartilage defects in the ovine stifle (knee)
Pierson, A; Kirschvink, Nathalie; Muylkens, B et al

in Proceedings du World Congress on osteoarthritis (2012)

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See detailIs evidence-based medicine so evident in veterinary research and practice? History, obstacles and perspectives.
Vandeweerd, Jean-Michel ULg; Kirschvink, Nathalie; Clegg, Peter et al

in Veterinary Journal (2012), 191(1), 28-34

Evidence-based medicine (EBM) refers to the conscientious, explicit and judicious use of current best evidence from research for the care of an individual patient. The concept of EBM was first described ... [more ▼]

Evidence-based medicine (EBM) refers to the conscientious, explicit and judicious use of current best evidence from research for the care of an individual patient. The concept of EBM was first described in human medicine in the early 1990s and was introduced to veterinary medicine 10 years later. However, it is not clear that the EBM approach promulgated in human medicine can be applied to the same extent to veterinary medicine. EBM has the potential to help veterinarians to make more informed decisions, but obstacles to the implementation of EBM include a lack of high quality patient-centred research, the need for basic understanding of clinical epidemiology by veterinarians, the absence of adequate searching techniques and accessibility to scientific data bases and the inadequacy of EBM tools that can be applied to the busy daily practise of veterinarians. This review describes the development of EBM in the veterinary profession, identifies its advantages and disadvantages and discusses whether and how veterinary surgeons should further adopt the EBM approach of human medicine. [less ▲]

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See detailCan beta2-adrenoceptor agonists, anticholinergic drugs, and theophylline contribute to the control of pulmonary inflammation and emphysema in COPD?
Zhang, Wen-Hui; Zhang, Yong; Cui, Yong-Yao et al

in Fundamental & Clinical Pharmacology (2012), 26(1), 118-134

Chronic obstructive pulmonary disease (COPD) has become a global epidemic disease with an increased morbidity and mortality in the world. Inflammatory process progresses and contributes to irreversible ... [more ▼]

Chronic obstructive pulmonary disease (COPD) has become a global epidemic disease with an increased morbidity and mortality in the world. Inflammatory process progresses and contributes to irreversible airflow limitation. However, there is no available therapy to better control the inflammatory progression and therefore to reduce the exacerbations and mortality. Thus, the development of efficient anti-inflammatory therapies is a priority for patients with COPD. beta(2) -Adrenoceptor agonists and anticholinergic agents are widely used as first line drugs in management of COPD because of their efficient bronchodilator properties. At present, many studies in vitro and some data obtained in laboratory animals reveal the potential anti-inflammatory effects of these bronchodilators but their protective role against chronic inflammation and the development of emphysema in patients with COPD remains to be investigated. The anti-inflammatory effects of theophylline at low doses have also been identified. Beneficial interactions between glucocorticoids and bronchodilators have been reported, and signaling pathways explaining these synergistic effects begin to be understood, especially for theophylline. Recent data demonstrating interactions between anticholinergics with beta(2) -adrenoceptor agonists aiming to better control the pulmonary inflammation and the development of emphysema in animal models of COPD justify the priority to investigate the interactive effects of a tritherapy associating corticoids with the two main categories of bronchodilators. [less ▲]

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See detailSystematic review of efficacy of nutraceuticals to alleviate clinical signs of osteoarthritis.
Vandeweerd, Jean-Michel ULg; Coisnon, C.; Clegg, P. et al

in Journal of Veterinary Internal Medicine (2012), 26(3), 448-56

BACKGROUND: Various treatments of osteoarthritis (OA) have been described, including use of nutraceuticals. OBJECTIVES: To review systematically the literature about the effects of nutraceuticals on ... [more ▼]

BACKGROUND: Various treatments of osteoarthritis (OA) have been described, including use of nutraceuticals. OBJECTIVES: To review systematically the literature about the effects of nutraceuticals on clinical signs of pain or abnormal locomotion in horses, dogs, and cats, and to discuss methodological aspects of trials and systematic reviews. METHODS: A systematic search of controlled trials evaluating the impact of nutraceuticals on OA in horses, dogs, and cats was performed, using Medline, CAB Abstracts, and Google Scholar. Scientific evidence was evaluated by means of criteria proposed by the Food and Drug Administration (FDA), and a scoring system adapted from both the CONsolidated Standards of Reporting Trials (CONSORT) statement and recommendations for assessing trials by the Center of Evidence Based Medicine of Oxford. RESULTS: Twenty-two papers were selected and reviewed, with 5 studies performed in horses, 16 in dogs, and 1 in cats. The strength of evidence was low for all nutraceuticals except for omega-3 fatty acid in dogs. There were limited numbers of rigorous randomized controlled trials and of participants in clinical trials. CONCLUSIONS AND CLINICAL IMPORTANCE: The evidence of efficacy of nutraceuticals is poor, with the exception of diets supplemented with omega-3 fatty acids in dogs. Greater access to systematic reviews must be part of the objectives of the veterinary science in the future. Their reporting would be improved by internationally agreed-upon criteria for standards and guidelines. [less ▲]

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See detailPrevalence of naturally occuring cartilage defects in the ovine stifle
Pierson, Audrey ULg; Muylkens, Benoît ULg; Hontoir, Fanny et al

Poster (2012)

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