References of "Garigliany, Mutien-Marie"
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See detailNew Hosts of The Lassa Virus.
Olayemi, Ayodeji; Cadar, Daniel; Magassouba, N. Faly et al

in Scientific Reports (2016), 6

Lassa virus (LASV) causes a deadly haemorrhagic fever in humans, killing several thousand people in West Africa annually. For 40 years, the Natal multimammate rat, Mastomys natalensis, has been assumed to ... [more ▼]

Lassa virus (LASV) causes a deadly haemorrhagic fever in humans, killing several thousand people in West Africa annually. For 40 years, the Natal multimammate rat, Mastomys natalensis, has been assumed to be the sole host of LASV. We found evidence that LASV is also hosted by other rodent species: the African wood mouse Hylomyscus pamfi in Nigeria, and the Guinea multimammate mouse Mastomys erythroleucus in both Nigeria and Guinea. Virus strains from these animals were isolated in the BSL-4 laboratory and fully sequenced. Phylogenetic analyses of viral genes coding for glycoprotein, nucleoprotein, polymerase and matrix protein show that Lassa strains detected in M. erythroleucus belong to lineages III and IV. The strain from H. pamfi clusters close to lineage I (for S gene) and between II &III (for L gene). Discovery of new rodent hosts has implications for LASV evolution and its spread into new areas within West Africa. [less ▲]

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See detailFeline panleukopenia virus in cerebral neurons of young and adult cats.
Garigliany, Mutien-Marie ULg; Gilliaux, Gautier ULg; Jolly, Sandra et al

in BMC veterinary research (2016), 12(1), 28

BACKGROUND: Perinatal infections with feline panleukopenia virus (FPV) have long been known to be associated with cerebellar hypoplasia in kittens due to productive infection of dividing neuroblasts. FPV ... [more ▼]

BACKGROUND: Perinatal infections with feline panleukopenia virus (FPV) have long been known to be associated with cerebellar hypoplasia in kittens due to productive infection of dividing neuroblasts. FPV, like other parvoviruses, requires dividing cells to replicate which explains the usual tropism of the virus for the digestive tract, lymphoid tissues and bone marrow in older animals. RESULTS: In this study, the necropsy and histopathological analyses of a series of 28 cats which died from parvovirus infection in 2013 were performed. Infections were confirmed by real time PCR and immunohistochemistry in several organs. Strikingly, while none of these cats showed cerebellar atrophy or cerebellar positive immunostaining, some of them, including one adult, showed a bright positive immunostaining for viral antigens in cerebral neurons (diencephalon). Furthermore, infected neurons were negative by immunostaining for p27(Kip1), a cell cycle regulatory protein, while neighboring, uninfected, neurons were positive, suggesting a possible re-entry of infected neurons into the mitotic cycle. Next-Generation Sequencing and PCR analyses showed that the virus infecting cat brains was FPV and presented a unique substitution in NS1 protein sequence. Given the role played by this protein in the control of cell cycle and apoptosis in other parvoviral species, it is tempting to hypothesize that a cause-to-effect between this NS1 mutation and the capacity of this FPV strain to infect neurons in adult cats might exist. CONCLUSIONS: This study provides the first evidence of infection of cerebral neurons by feline panleukopenia virus in cats, including an adult. A possible re-entry into the cell cycle by infected neurons has been observed. A mutation in the NS1 protein sequence of the FPV strain involved could be related to its unusual cellular tropism. Further research is needed to clarify this point. [less ▲]

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See detailReconstruction of the Evolutionary History and Dispersal of Usutu Virus, a Neglected Emerging Arbovirus in Europe and Africa.
Engel, Dimitri; Jost, Hanna; Wink, Michael et al

in mBio (2016), 7(1),

Usutu virus (USUV), one of the most neglected Old World encephalitic flaviviruses, causes epizootics among wild and captive birds and sporadic infection in humans. The dynamics of USUV spread and ... [more ▼]

Usutu virus (USUV), one of the most neglected Old World encephalitic flaviviruses, causes epizootics among wild and captive birds and sporadic infection in humans. The dynamics of USUV spread and evolution in its natural hosts are unknown. Here, we present the phylogeny and evolutionary history of all available USUV strains, including 77 newly sequenced complete genomes from a variety of host species at a temporal and spatial scaled resolution. The results showed that USUV can be classified into six distinct lineages and that the most recent common ancestor of the recent European epizootics emerged in Africa at least 500 years ago. We demonstrated that USUV was introduced regularly from Africa into Europe in the last 50 years, and the genetic diversity of European lineages is shaped primarily by in situ evolution, while the African lineages have been driven by extensive gene flow. Most of the amino acid changes are deleterious polymorphisms removed by purifying selection, with adaptive evolution restricted to the NS5 gene and several others evolving under episodic directional selection, indicating that the ecological or immunological factors were mostly the key determinants of USUV dispersal and outbreaks. Host-specific mutations have been detected, while the host transition analysis identified mosquitoes as the most likely origin of the common ancestor and birds as the source of the recent European USUV lineages. Our results suggest that the major migratory bird flyways could predict the continental and intercontinental dispersal patterns of USUV and that migratory birds might act as potential long-distance dispersal vehicles. IMPORTANCE: Usutu virus (USUV), a mosquito-borne flavivirus of the Japanese encephalitis virus antigenic group, caused massive bird die-offs, mostly in Europe. There is increasing evidence that USUV appears to be pathogenic for humans, becoming a potential public health problem. The emergence of USUV in Europe allows us to understand how an arbovirus spreads, adapts, and evolves in a naive environment. Thus, understanding the epidemiological and evolutionary processes that contribute to the emergence, maintenance, and further spread of viral diseases is the sine qua non to develop and implement surveillance strategies for their control. In this work, we performed an expansive phylogeographic and evolutionary analysis of USUV using all published sequences and those generated during this study. Subsequently, we described the genetic traits, reconstructed the potential pattern of geographic spread between continents/countries of the identified viral lineages and the drivers of viral migration, and traced the origin of outbreaks and transition events between different hosts. [less ▲]

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See detailN-Acetylcysteine: An Old Drug With Variable Anti-Influenza Properties
Casanova, Tomas; Garigliany, Mutien-Marie ULg

in Journal of Controversies in Biomedical Research (2016)

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See detailRisk factors and effect of selective removal on retroviral infections prevalence in Belgian stray cats.
Garigliany, Mutien-Marie ULg; Jolly, Sandra ULg; Dive, M. et al

in The Veterinary record (2016), 178(2), 45

The aim of this study was to evaluate the effect of several risk/protective factors and predictors on the prevalence of feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) infections in ... [more ▼]

The aim of this study was to evaluate the effect of several risk/protective factors and predictors on the prevalence of feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) infections in 302 stray cats captured during a trap-neuter-release programme in a mixed urban-rural area from Belgium, from 2010 to 2012. The impact of selective removal of FIV-positive cats on the apparent prevalence in the remaining population over this three-year period was also assessed. The seroprevalences over three years were 18.8 per cent for FIV and 0.7 per cent for FeLV. For FIV, the seroprevalence decreased significantly from the first year of the programme (2010; 30.5 per cent) to the last (2012; 13.1 per cent). Sex (male) and age (adult and old cats) were risk factors, while the year of sampling (years 2011 and 2012) was a protective factor. Age, sex and location were the most relevant predictors of FIV status. The data presented in this study revealed a very high FIV seroprevalence in Belgian stray cats, while FeLV was almost absent. The selective removal of positive cats had a drastic effect on the FIV seroprevalence in the remaining cat population. [less ▲]

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See detailHyporeactivity of Alveolar Macrophages and Higher Respiratory Cell Permissivity Characterize DBA/2J Mice Infected by Influenza A Virus Hyporeactivity of Alveolar Macrophages and Higher Respiratory Cell Permissivity Characterize DBA/2J Mice Infected by Influenza A Virus.
Casanova Bustos, Tomas Ronaldo ULg; Van de Paar, Els; Desmecht, Daniel ULg et al

in JOURNAL OF INTERFERON & CYTOKINE RESEARCH (2015), 35(10), 808-820

Influenza A virus remains a major public health problem. Mouse models have been widely used to study influenza infection in mammals. DBA/2J and C57BL/6J represent extremes in terms of susceptibility to ... [more ▼]

Influenza A virus remains a major public health problem. Mouse models have been widely used to study influenza infection in mammals. DBA/2J and C57BL/6J represent extremes in terms of susceptibility to influenza A infection among inbred laboratory mouse strains. Several studies focused specifically on the factors responsible for the susceptibility of DBA/2J or the resistance of C57BL/6J and resulted in impressive lists of candidate genes or factors over- or underexpressed in one of the strains. We adopted a different phenotypical approach to identify the critical steps of the infection process accounting for the differences between DBA/2J and C57BL/6J strains. We concluded that both a dysfunction of alveolar macrophages and an increased permissivity of respiratory cells rendered DBA/2J more susceptible to influenza infection. [less ▲]

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See detailPutative Lineage of Novel African Usutu Virus, Central Europe.
Cadar, Daniel; Bosch, Stefan; Jost, Hanna et al

in Emerging infectious diseases (2015), 21(9), 1647-50

We characterized the complete genome of a putative novel Usutu virus (USUV) strain (Usutu-BONN) detected in a dead blackbird from Germany. Genomic analysis revealed several unique amino acid substitutions ... [more ▼]

We characterized the complete genome of a putative novel Usutu virus (USUV) strain (Usutu-BONN) detected in a dead blackbird from Germany. Genomic analysis revealed several unique amino acid substitutions among the polyprotein gene. Phylogenetic analyses demonstrated that Usutu-BONN constitutes a putative novel African USUV lineage, which was probably recently introduced to central Europe. [less ▲]

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See detailCyclovirus CyCV-VN species distribution is not limited to Vietnam and extends to Africa
Garigliany, Mutien-Marie ULg; Hagen, Ralf Mathias; Frickmann, Hagen et al

in Scientific Reports (2014), 4

Cycloviruses, small ssDNA viruses of the Circoviridae family, have been identified in the cerebrospinal fluid from symptomatic human patients. One of these species, cyclovirus-Vietnam (CyCV-VN), was shown ... [more ▼]

Cycloviruses, small ssDNA viruses of the Circoviridae family, have been identified in the cerebrospinal fluid from symptomatic human patients. One of these species, cyclovirus-Vietnam (CyCV-VN), was shown to be restricted to central and southern Vietnam. Here we report the detection of CyCV-VN species in stool samples from pigs and humans from Africa, far beyond their supposed limited geographic distribution. [less ▲]

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See detailAlveolar macrophages hyporeactivity act in the extreme susceptibility of DBA/2J to Influenza A infection.
Casanova Bustos, Tomas Ronaldo ULg; Garigliany, Mutien-Marie ULg; Desmecht, Daniel ULg

Poster (2014, October 17)

DBA/2J and C57BL/6J represent two opposite ends in terms of sensitivity and resistance to influenza A virus between the Mx-negative mouse lines. Several research teams focused on the factors explaining ... [more ▼]

DBA/2J and C57BL/6J represent two opposite ends in terms of sensitivity and resistance to influenza A virus between the Mx-negative mouse lines. Several research teams focused on the factors explaining this difference, mainly by genetic approaches using Recombinant Inbred Lines between those two strains. Several candidate-genes have been proposed, but it was not possible to determine their importance. To help to define the factors involved in the susceptibility of DBA/2J mice to influenza infection, we adopted a phenotypic approach to identify the critical steps of the infection process accounting for this extreme susceptibility. Overall, the data presented here support the role of a dysfunction of alveolar macrophages, to influenza infection in the higher susceptibility of DBA/2J mice to this virus. [less ▲]

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See detailNatural intrauterine infection with Schmallenberg virus in malformed newborn calves: pathology and distribution of viral RNA
Bayrou, Calixte ULg; Garigliany, Mutien-Marie ULg; Sarlet, Michaël ULg et al

in Emerging Infectious Diseases (2014), 20(8),

We comprehensively surveyed morphologic alterations in calves naturally infected in utero by Schmallenberg virus (SBV) and born deformed. SBV-specific RNA was distributed unevenly in different tissues ... [more ▼]

We comprehensively surveyed morphologic alterations in calves naturally infected in utero by Schmallenberg virus (SBV) and born deformed. SBV-specific RNA was distributed unevenly in different tissues. Implications for diagnosic procedures are highlighted. [less ▲]

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See detailEstudio de las citoquinas producidas por los macrófagos alveolares de los ratones DBA/2J y C57BL/6J en el curso de la infección por el Virus Influenza A, modelo ex vivo.
Casanova Bustos, Tomas Ronaldo ULg; Garigliany, Mutien-Marie ULg; Desmecht, Daniel ULg

Poster (2014, June 18)

DBA/2J and C57BL/6J represent two opposite ends in terms of sensitivity and resistance to influenza A virus between the Mx-negative mouse lines. Several research teams focused on the factors explaining ... [more ▼]

DBA/2J and C57BL/6J represent two opposite ends in terms of sensitivity and resistance to influenza A virus between the Mx-negative mouse lines. Several research teams focused on the factors explaining this difference, mainly by genetic approaches using Recombinant Inbred Lines between those two strains. Several candidate-genes have been proposed, but it was not possible to determine their importance. To help to define the factors involved in the susceptibility of DBA/2J mice to influenza infection, we adopted a phenotypic approach to identify the critical steps of the infection process accounting for this extreme susceptibility. Overall, the data presented here support the role of a dysfunction of alveolar macrophages, to influenza infection in the higher susceptibility of DBA/2J mice to this virus. [less ▲]

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See detailDetection of Usutu virus in a bullfinch (Pyrrhula pyrrhula) and a great spotted woodpecker (Dendrocopos major) in north-west Europe
Garigliany, Mutien-Marie ULg; Marlier, Didier ULg; Tenner-Racz, Klara et al

in Veterinary Journal (2014), 199

In October 2012, a 3-year-old bullfinch (Pyrrhula pyrrhula) held in captivity for its entire lifespan and a wild adult great spotted woodpecker (Dendrocopos major), both with neurological signs, were ... [more ▼]

In October 2012, a 3-year-old bullfinch (Pyrrhula pyrrhula) held in captivity for its entire lifespan and a wild adult great spotted woodpecker (Dendrocopos major), both with neurological signs, were found 4 km from each other and 5 days apart in the Meuse Valley, Belgium. Non-suppurative encephalitis and mild degeneration and necrosis were identified in the brain and cerebellum, and Usutu virus antigen and RNA were detected by immunohistochemistry and real-time reverse transcriptase PCR, respectively. The two cases reported here represent the most western distribution of clinical disease in birds due to Usutu virus. [less ▲]

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See detailCharacterization of a novel circo-like virus in Aedes vexans mosquitoes from Germany: evidence for a new genus within the Circoviridae family.
Garigliany, Mutien-Marie ULg; Borstler, Jessica; Jost, Hanna et al

in The Journal of general virology (2014)

During the last decades, metagenomic studies expanded the numbers of newly described, often unclassified, viruses within the Circoviridae family. Using broad-spectrum circo-/cyclovirus PCRs, we ... [more ▼]

During the last decades, metagenomic studies expanded the numbers of newly described, often unclassified, viruses within the Circoviridae family. Using broad-spectrum circo-/cyclovirus PCRs, we characterized a novel circo-like virus in Aedes vexans mosquitoes from Germany whose main putative open reading frames (ORFs) shared very low amino acid identity with those of previously characterized circo-/cycloviruses. Phylogenetic and genetic distance analysis revealed that this new virus species defines, with previously described mosquito- and bat feces-derived circo-like viruses, a different genus, tentatively called "krikovirus", within Circoviridae. We further demonstrated that viruses of the putative krikovirus genus all share a genomic organization which is unique among Circoviridae. Further investigations are needed to determine the host range, tissue tropism and transmission route(s). This report increases the current knowledge of the genetic diversity and evolution of the members of the Circoviridae family. [less ▲]

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See detailBluetongue Virus RNA Detection by Real-Time RT-PCR in Post-Vaccination Samples from Cattle
De Leeuw, Ilse; Garigliany, Mutien-Marie ULg; Bertels, Guido et al

Conference (2013, October 02)

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See detailNo serologic evidence for emerging Schmallenberg virus infection in dogs (Canis domesticus)
Garigliany, Mutien-Marie ULg; Desmecht, Daniel ULg; Bayrou, Calixte ULg et al

in Vector Borne & Zoonotic Diseases (2013), 13(11), 1-4

Schmallenberg virus, a novel orthobunyavirus, is spreading among ruminants, especially sheep and cattle throughout Europe. To determine the risk for domestic dog infection, we conducted a survey among ... [more ▼]

Schmallenberg virus, a novel orthobunyavirus, is spreading among ruminants, especially sheep and cattle throughout Europe. To determine the risk for domestic dog infection, we conducted a survey among cases referred to the university Companion Animal Clinic to assess possible seroconversion. No evidence of transmission to dogs was detected. [less ▲]

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See detailSchmallenberg virus circulation among red and roe deer populations in Belgium
Garigliany, Mutien-Marie ULg; Volpe, Rosario ULg; Paternostre, Julien ULg et al

in 31th Congress of the International Union of Game Biologists, Abstract Book (2013, August 27)

Schmallenberg virus (SBV) is a recently discovered vector-borne Orthobunyavirus targeting ruminants. It is transmitted by Culicoides biting midges and caused a large outbreak in European sheep and cattle ... [more ▼]

Schmallenberg virus (SBV) is a recently discovered vector-borne Orthobunyavirus targeting ruminants. It is transmitted by Culicoides biting midges and caused a large outbreak in European sheep and cattle populations in 2011 and 2012. The infection of adults was associated with a drop in milk production, fever and diarrhea. But the virus was further shown to cross the placental barrier and to be responsible for a hydrocephaly/arthrogryposis syndrome in calves and lambs. After its occurrence in 2011 in Germany, SBV quickly spread across Europe and in spring 2012 more than 90% of Belgian domestic cattle had seroconverted. To assess the susceptibility of wild ruminants to the infection, a total number of 547 and 494 sera, from red (Cervus elaphus) and roe deer (Capreolus capreolus), respectively, were collected during the hunting seasons 2010 to 2012 and tested for the presence of anti-SBV antibodies. While no samples from 2010 revealed to be positive, about two-thirds of red deer and half of roe deer sampled in 2011 were seropositive. In 2012, the seroprevalence dropped to 33% in red deer and remained stable in roe deer. The high seroprevalence rates found in both species in Belgium shows that wild ruminants are susceptible to the infection by SBV. If the infection of deer was associated to a hydrocephaly/arthrogryposis syndrome similar to that observed in domestic ruminants is still unknown. There is currently no evidence of such a transplacental passage in red or roe deer. The decrease in the seroprevalence observed in red deer in 2012 might be the result of the turn-over in the red deer population and reflect an absence of virus circulation in 2012. Further investigations in the upcoming years will help to enlighten this point. [less ▲]

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See detailDetection of antibodies against Schmallenberg virus in wild boars, Belgium, 2010-2012
Desmecht, Daniel ULg; Garigliany, Mutien-Marie ULg; Beer, Martin et al

in Lecoq, Yves (Ed.) 31th Congress of the International Union of Game Biologists (2013, August 27)

In the summer/fall of 2011, a nonspecific febrile syndrome characterized by hyperthermia and drop in milk production with occasional reports of watery diarrhea and abortion was reported among dairy cows ... [more ▼]

In the summer/fall of 2011, a nonspecific febrile syndrome characterized by hyperthermia and drop in milk production with occasional reports of watery diarrhea and abortion was reported among dairy cows on farms in northwestern Europe. Further, in November 2011, an enzootic outbreak of malformed neonates emerged in several European countries, with stillbirth and birth at term of lambs, kids and calves with neurological signs or malformations of the head, spine, or limbs. Both syndromes were associated with the presence in the blood (adults) or in the central nervous system (newborns) of a new Shamonda/Sathuperi-like orthobunyavirus, provisionally named Schmallenberg virus (SBV) after the town in Germany where the first positive clinical samples were identified. Defining as precisely as possible the host range of the newcomer is a key point to predict the outcome of the emergence of SBV disease in Europe. In this respect, it must be pointed out that orthobunyaviruses infect more animal species than those in which the foetus is damaged. Recently, serological evidence for SBV infection in wild ruminant species (Cervus elaphus and Capreolus capreolus) was reported (Linden et al., 2012). In the present study, the objective was to seek after serological evidence of SBV infection among wild boars living in a geographical area where exposure to infected insect vectors was high in 2011, as judged from the very high seroprevalence reported among cattle in that region. About 700 animals were sampled during the 2010-2012 hunting seasons. All serum samples collected during the fall of 2010 were seronegative. On the contrary, apparent seroprevalence among wild boars in 2011 was ~27% and started to decline in 2012 (~11%). Acquired immunity against the new virus was thus already very high in the wild boar populations sampled in the fall 2011, suggesting that the new virus had quickly spread throughout the region since its emergence about 250 km northeast in the late summer 2011. The drop in seroprevalence recorded in 2012 suggests that the virus was no more circulating in the region. [less ▲]

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