Isoform 111 of vascular endothelial growth factor (VEGF111) improves angiogenesis of ovarian tissue xenotransplantation
Labied, Soraya ; Delforge, Yves ; Munaut, Carine et al
in Transplantation (2013), 95(3), 426-433
Background: Cryopreservation of cortex ovarian tissue before anti-cancer therapy is a promising technique for fertility preservation mainly in children and young women. Ischemia in the early stage after ... [more ▼]
Background: Cryopreservation of cortex ovarian tissue before anti-cancer therapy is a promising technique for fertility preservation mainly in children and young women. Ischemia in the early stage after ovarian graft causes massive follicle loss by apoptosis. VEGF111 is a recently described VEGF isoform that does not bind to the extracellular matrix, diffuse extensively and is resistant to proteolysis. These properties confer a significantly higher angiogenic potential to VEGF111 in comparison to the other VEGF isoforms. Methods: We evaluated the morphology of cryopreserved sheep ovarian cortex, grafted in the presence or absence of VEGF111. Ovarian cortex biopsies were embedded in type I collagen with or without VEGF111 addition before transplantation to SCID mice ovaries. Transplants were retrieved 3 days or 3 weeks later. Follicular density, vasculature network, haemoglobin content and cell proliferation were analysed. Results: Addition of VEGF111 increased density of functional capillaries (p=0.01) 3 days after grafting. By double immunostaining of Ki-67 and von Willebrand Factor (vWF) we demonstrated that proliferating endothelial cells were found in 83% of the VEGF111 group when compared to 33% in the control group (p=0.001). This angio-stimulation was associated with a significant enhancement of haemoglobin content (p=0.03). Three weeks after transplantation, the number of primary follicles was significantly higher in VEGF111 grafts (p=0.02). Conclusion: VEGF111 accelerates blood vessels recruitment, functional angiogenesis and improves the viability of ovarian cortex by limiting ischemia and ovarian cortex damage. [less ▲]Detailed reference viewed: 34 (12 ULg)
Use of Sheep Ovarian Tissue as a Model to Restore Fertility in Young Cancerous Women
Fransolet, Maïté ; HENRY, Laurie ; Rozet, Eric et al
Poster (2012, December 10)Detailed reference viewed: 53 (13 ULg)
Does vascular endothelial growth factor improve ovarian tissue recovery after cryopreservation?
Henry, Laurie ; Fransolet, Maïté ; Labied, Soraya et al
in Giornale italiano di obstetricia e gynecologia (2012)Detailed reference viewed: 17 (2 ULg)
Does isoforms 111 and 165 of vascular endothelial growth factor (VEGF111 and 165) improve transplanted ovarian tissue survival?
Henry, Laurie ; Fransolet, Maïté ; LABIED, Soraya et al
in Journal of Assisted Reproduction & Genetics (2011), 28Detailed reference viewed: 31 (13 ULg)