References of "Fraipont, Audrey"
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See detailExpression microarray as a tool to identify candidate blood biomarkers in horses suffering from inflammatory airway disease
Ramery, Eve ULg; Fraipont, Audrey ULg; Art, Tatiana ULg et al

in Veterinary Clinical Pathology (in press)

Background: Inflammatory airway disease (IAD) affects performance and well-being in horses. Diagnosis is primarily reached by bronchoalveolar lavage (BAL) cytology but this is invasive and requires ... [more ▼]

Background: Inflammatory airway disease (IAD) affects performance and well-being in horses. Diagnosis is primarily reached by bronchoalveolar lavage (BAL) cytology but this is invasive and requires sedation. Objectives: The purpose of this study was to identify candidate blood biomarkers of IAD using species-specific expression microarrays. Methods: Horse Gene Expression Microarrays were used to investigate global mRNA expression in circulating leukocytes from healthy and IAD-affected standardbreds and endurance horses. Results: Nine genes were significantly differentially regulated in standardbreds and 61 in endurance horses (P < 0.001). These genes were mainly related to inflammation (eg. ALOX15B, PLA2G12B and PENK), oxidant/antioxidant balance (eg. DUOXA2 and GSTO1-1) and stress (eg. V1aR, GRLF1, Homer-2 and MAOB). DUOXA2, ALOX15B, PLA2G12B, MAOB and GRLF1 variations of expression were further validated by RT-qPCR. The deregulation of the oxidant/antioxidant balance was demonstrated at the protein level by an increase of glutathione peroxidase (GPx) activity in heparinised whole blood of IAD-affected standardbreds (P = 0.0025) and endurance horses (P = 0.0028). There was good correlation (r = 0.7354) between BAL neutrophil percentage and whole blood GPx activity in all horses. Conclusions: There is accumulating evidence that, even when systemic clinical signs are not evident, circulating leukocyte gene expression can reflect responses of other tissues, leading to potential diagnostic applications in the future. Although not specific for IAD, whole blood GPx activity appears to reflect BAL neutrophil percentage. This finding should be further assessed by testing a larger number of horses. [less ▲]

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See detailÉtiopathogénie de la myopathie atypique et gestion raisonnée des cas
Votion, Dominique ULg; Patarin, Florence; Cerri, Simona ULg et al

in In Proceedings: Journée AVEF (Association des Vétérinaires Equins Français) (2013, December)

Atypical myopathy is an acute intoxication resulting from the ingestion of a toxin contained in the seeds of some trees of the genus Acer (i.e. « maple trees »). The causes of the emergence of the disease ... [more ▼]

Atypical myopathy is an acute intoxication resulting from the ingestion of a toxin contained in the seeds of some trees of the genus Acer (i.e. « maple trees »). The causes of the emergence of the disease due to trees naturalized for many decades remain undetermined. Currently, there is no cure for atypical myopathy but symptomatic treatment may be revisited on the basis of knowledge of the aetio-pathogenesis of the disease. This paper compiles recommendations following a discussion on treatment of atypical myopathy held in October 2013 at the University of Liege (Belgium) to provide emergency treatment distinguishing clinical management (1) at hospital, (2) in the field and (3) preventive measures to protect pasture companions. [less ▲]

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See detailAssessing fitness in endurance horses
Fraipont, Audrey ULg; Van Erck, Emmanuelle; Ramery, Eve ULg et al

in Canadian Veterinary Journal = Revue Vétérinaire Canadienne (2012), 53

A field test and a standardized treadmill test were used to assess fitness in endurance horses. These tests discriminated horses of different race levels: horses participating in races of 120km and more ... [more ▼]

A field test and a standardized treadmill test were used to assess fitness in endurance horses. These tests discriminated horses of different race levels: horses participating in races of 120km and more showed higher values of VLA4 (velocity at which blood lactate reached 4 mmol/L) and V200 (velocities at which heart rates reached 200 beats per min) than horses of lower race levels. [less ▲]

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See detailMesure de la respiration musculaire par respirométrie à haute résolution
Votion, Dominique ULg; Fraipont, Audrey ULg; Robert, Céline et al

in Pratique Vétérinaire Equine (2011), 43(170), 35-37

This technical note describes the reference method for screening the mitochondrial function in horses.

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See detailAltered diastolic and systolic left ventricular function in horses completing a long distance endurance race.
Amory, Hélène ULg; Votion, Dominique ULg; Fraipont, Audrey ULg et al

in In Proccedings of the First Benelux Congress of Physiology and Pharmacology (PHYSPHAR) (2011, March 18)

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See detailSubclinical diseases underlying poor performance in endurance horses: diagnostic methods and predictive tests.
Fraipont, Audrey ULg; Van Erck, E.; Ramery, Eve ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2011), 169(6), 154

Thirty-eight endurance horses underwent clinical and ancillary examinations, including haematological and biochemical evaluation, standardised exercise tests both on a treadmill and in the field, Doppler ... [more ▼]

Thirty-eight endurance horses underwent clinical and ancillary examinations, including haematological and biochemical evaluation, standardised exercise tests both on a treadmill and in the field, Doppler echocardiography, impulse oscillometry, video endoscopy and collection of respiratory fluids. All of the examined poorly performing horses were affected by subclinical diseases, and most of them had multiple concomitant disorders. On the contrary, the well-performing horses were free of any subclinical disease. The most frequently diagnosed diseases were respiratory disorders, followed by musculoskeletal and cardiac problems. Poor performers exhibited lower speeds at blood lactate concentration of 4 mmol/l (VLA4) and at heart rates of 160 (V160) and 200 bpm (V200) on the treadmill and in the field, as well as slower recovery of heart rate. [less ▲]

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See detailCharacterization of pentraxin 3 in the horse and its expression in airways.
Ramery, Eve ULg; Fievez, Laurence ULg; Fraipont, Audrey ULg et al

in Veterinary Research (2010), 41(2), 18

The long pentraxin 3 (PTX3) plays an important role in host defence and its over-expression may contribute to airway injury. The aim of the present study was therefore to characterize in more detail PTX3 ... [more ▼]

The long pentraxin 3 (PTX3) plays an important role in host defence and its over-expression may contribute to airway injury. The aim of the present study was therefore to characterize in more detail PTX3 and its expression in the horses airway. Six healthy horses and six horses affected by recurrent airway obstruction (R.A.O.) were submitted to a dusty environment challenge. PTX3 DNA and cDNA were cloned and sequenced. PTX3 expression was evaluated by RT-qPCR, western blotting and immuno-histochemistry in bronchoalveolar lavage fluid (BALF) cells, BALF supernatant and bronchial epithelial cells. An alternative splicing of the second exon of PTX3 occurred, resulting in two forms of the protein: spliced (32 kDa) and full length (42 kDa). PTX3 was detected in BALF macrophages, neutrophils and bronchial epithelial cells. It was over-expressed in the BALF supernatant from R.A.O.-affected horses in crisis. However, dust was unable to induce PTX3 in BALF cells ex vivo, indicating that dust is an indirect inducer of PTX3. Dust exposure in-vivo induced PTX3 in BALF macrophages but there was no significant difference between healthy and R.A.O.-affected horses. Conversely, PTX3 was over-expressed in the bronchial epithelial cells from R.A.O-affected horses in crisis. These data indicate a differential regulatory mechanism in inflammatory and bronchial epithelial cells and offer therapeutically interesting perspectives. [less ▲]

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See detailAltered diastolic and systolic left ventricular function in horses completing a long distance endurance race
Amory, Hélène ULg; Votion, Dominique ULg; Fraipont, Audrey ULg et al

in In Proceedings of the 8th International Conference on Equine Exercise Physiology (ICEEP) (2010)

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See detailLa microbiopsie musculaire : un nouvel outil pour le suivi sportif et la détection précoce des dysfonctions musculaires
Votion, Dominique ULg; Fraipont, Audrey ULg; Robert, Céline et al

in 36ème Journée de la Recherche Equine (2010)

The objective of this study was to confirm the practical value of high-resolution respirometry (HRR) applied to biopsies to determine, in horses, the level of training, their athletic ability and for the ... [more ▼]

The objective of this study was to confirm the practical value of high-resolution respirometry (HRR) applied to biopsies to determine, in horses, the level of training, their athletic ability and for the early detection of muscular dysfunction. Materials and methods – The muscle mitochondrial respiration was determined by HRR in 20 endurance horses and 10 trotters sampled at the triceps brachii at different stages of their training. Results – Training increases mitochondrial respiration, in addition, the best performers had the highest rate of respiration. A trotter had abnormally low levels of muscle mitochondrial respiration for its level of training. This horse has presented several episodes of rhabdomyolysis during its racing season. Discussion – The biopsy is easily achievable by the attending veterinarian. This study shows that the athletic ability of horses is closely linked to respiratory muscle function, and suggests the value of HRR for performance prediction. In addition, the RHR has the ability to demonstrate mitochondrial dysfunction potentially responsible for exercise-induced rhabdomyolysis. [less ▲]

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See detailPulmonary function, airway cytology and bronchoalveolar lavage fluid drug concentration after aerosol administration of cefquinome to horses
Art, Tatiana ULg; Ramery, Eve ULg; Fraipont, Audrey ULg et al

in Equine Veterinary Education (2010), 22(9), 473-479

The administration of antibiotics by aerosol to horses suffering from respiratory infections may partially circumvent the limitations of antimicrobial therapy, e.g. large injection volumes, low ... [more ▼]

The administration of antibiotics by aerosol to horses suffering from respiratory infections may partially circumvent the limitations of antimicrobial therapy, e.g. large injection volumes, low bioavailability and risk of diarrhea. Only injectable formulations are available currently and usually contain other substances that could irritate the mucosa and induce coughing and bronchospasm. In addition, the quality of the aerosol, particularly in terms of the delivery of antibiotics to the deep parts of the lung, is unknown. Although used under field conditions, cefquinome delivered by aerosol has never been studied in horses. This study examined the safety of cefquinome injectable solution, administered by aerosol at a dose of 225 mg/inhalation to 7 healthy horses, by assessing (1) pulmonary function before and 15 min after a single inhalation, at the first day (Day 1) and the fifth day (Day 5) of a 5 day period treatment; and (2) the inflammatory status of the lung, i.e. percentage neutrophils and myeloperoxidase concentration, based on bronchoalveolar lavage (BAL) at D1 and D5. In addition, cefquinome concentrations were measured in bronchoalveolar lavage fluid after aerosol, intravenous (i.v.) and intramuscular (i.m.) administrations. A single aerosol of cefquinome injectable solution did not induce any immediate nor delayed pulmonary side effects in healthy horses and produced cefquinome concentrations in bronchoalveolar lavage (BAL) within 30 min that were higher than the minimal inhibitory concentration of the main equine respiratory pathogens. These results should stimulate further studies, especially in horses suffering from bronchial hyper-reactivity. Aerosol delivery of antibiotics may well have a role in equine therapeutics. [less ▲]

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See detailHydratation and electrolyte balance in horses during an endurance season
Robert, Céline; Goachet, Anne-Gaëlle; Fraipont, Audrey ULg et al

in Equine Veterinary Journal. Supplement (2010), 42(38), 98-104

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See detailAlterations in mitochondrial respiratory function in response to endurance training and endurance racing
Votion, Dominique ULg; Fraipont, Audrey ULg; Goachet, Anne-Gaëlle et al

in Equine Veterinary Journal. Supplement (2010), 42(38), 268-274

Objectives: To determine effects of training and racing on muscle oxidative phosphorylation (OXPHOS) and electron transport system (ETS) capacities in horses with high-resolution respirometry (HRR).

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See detailIntérêt du suivi sportif à la piste des chevaux d’endurance dans la détection des problèmes subcliniques
Fraipont, Audrey ULg; Van Erck, Emmanuelle; Art, Tatiana ULg et al

in 35ème Journée de la Recherche équine (2009)

The aim of this study was to evaluate a field exercise test specifically designed for endurance horses and to determine the relevant parameters allowing discrimination between healthy horses (H) and ... [more ▼]

The aim of this study was to evaluate a field exercise test specifically designed for endurance horses and to determine the relevant parameters allowing discrimination between healthy horses (H) and horses presenting subclinical disorders (SP). Twenty four endurance horses underwent blood analyses, general examination and special exams to define a posteriori 2 groups: group H composed of healthy horses (n=12) and group SP included horses presenting subclinical disorders (n=12). The exercise test consisted of a warm-up followed by 3 gallop steps (27 km, 1.5km, 1.5km) at incremental speed. Lactates were measured after each step, an ECG was recorded during effort and recovery, speed was monitored by a GPS. Significant differences were observed between H and SP groups for VLA4, V160 and heart rate during recovery with better values for H horses. These results demonstrate the pertinence of a medical athletic follow-up in endurance horses: this field exercise test is easily achievable and allows the detection of subclinical disorders which would otherwise remain unsuspected by the riders. [less ▲]

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See detailLe microdamier: un outil du futur pour le diagnostic de l’inflammation ?
Mignot, Clémence ULg; Fraipont, Audrey ULg; Richard, Eric et al

Poster (2009)

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See detailEffect de l'entraînement sur la fonction mitochondriale musculaire du cheval d'endurance
Votion, Dominique ULg; Fraipont, Audrey ULg; Franck, Thierry ULg et al

in 35eme Journee de la Recherche Equine, jeudi 26 fevrier 2009, Paris, France (2009)

Athletic ability of endurance horses is intimately linked to muscle oxidative capacity. Microbiopsies of triceps brachii (TB) and gluteus medius (GM) were collected before and after 10 weeks of training ... [more ▼]

Athletic ability of endurance horses is intimately linked to muscle oxidative capacity. Microbiopsies of triceps brachii (TB) and gluteus medius (GM) were collected before and after 10 weeks of training in 7 endurance horses to assess the feasibility of studying training effect with the use of microbiopsies. Oxygen consumption of permeabilized fibers was evaluated by high resolution respirometry (HRR) with a titration protocol that defines the activity of the mitochondrial complexes. Differences among muscles before and after training as well as training effect were assessed by Wilcoxon matched pairs test (P<0.05). No complications occurred following microbiopsies in any horse. The in situ oxidative capacity of the TB and GM increased with training. No difference was found between the TB and GM when one horse was excluded from the statistical analysis. Indeed, this horse showed signs of exercise intolerance before sampling (after the training period) which were associated to a low rate of respiration in GM but not in TB. Results of this study showed that HRR may be used to follow training effect and suggest that metabolic impairment might be detected with HRR. [less ▲]

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See detailAssociation between lower airway inflammation and EIPH in 196 standarbred racehorses
Simon, Valérie ULg; Art, Tatiana ULg; Ramery, Eve ULg et al

in Tessier, Caroline; Gerber, Vincent (Eds.) Abstract book: 4th World Equine Airways Symposium (WEAS) (2009)

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See detailAerosol administration of cefquinome in healthy horses – effect on pulmonary function and lung inflammation
Art, Tatiana ULg; Ramery, Eve ULg; Fraipont, Audrey ULg et al

in Tessier, Caroline; Gerber, Vincent (Eds.) Abstract book: 4th World Equine Airways Symposium (WEAS) (2009)

Detailed reference viewed: 67 (7 ULg)