References of "Dal Pozzo, Fabiana"
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See detailSerogroups and genotypes of Leptospira 1 spp. strains from bovine aborted fetuses
Delooz, L; Czaplicki, G; Grégoire, F et al

in Transboundary and Emerging Diseases (in press)

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See detailINFECTIVITÉ IN VIVO ET RÉCUPÉRATION IN VITRO DE L’APTITUDE (FITNESS) RÉPLICATIVE D’UN NOROVIRUS MURIN RECOMBINANT
De Oliveira-Filho, Edmilson; Ludwig, Louisa ULg; Mathijs, Elisabeth et al

Scientific conference (2017, March)

Les norovirus sont reconnus comme étant une cause majeure d’épidémies de gastroentérites chez l’homme. Les mutations ponctuelles et la recombinaison sont les mécanismes moléculaires moteurs de l’évolution ... [more ▼]

Les norovirus sont reconnus comme étant une cause majeure d’épidémies de gastroentérites chez l’homme. Les mutations ponctuelles et la recombinaison sont les mécanismes moléculaires moteurs de l’évolution des norovirus. La recombinaison peut créer des modifications considérables dans les génomes viraux. Nous avons récemment obtenu un norovirus murin recombinant (RecMNV) après coinfection in vitro de souches homologues de norovirus murins. Le RecMNV présente une aptitude (fitness) réduite à la réplication. L'infectivité du RecMNV a été d’abord évaluée in vivo et ses adaptations réplicatives et génétiques ont été ensuite suivies au cours de passages en série in vitro. Durant l'infection in vivo, le RecMNV a été comparé aux souches parentales chez des souris Balb/cByJ par la mesure du poids corporel et l'estimation de la charge virale par RT-qPCR. Le poids des souris infectées par RecMNV était généralement plus élevé que celui des souris infectées par les souches parentales. Des charges virales ont été détectées dans tous les organes examinés, suggérant que, comme les souches parentales, le RecMNV peut se propager au-delà du tube digestif pour produire des infections systémiques. Ensuite, les générations de RecMNV résultant du premier (RecE) et du dixième (RecL) passages in vitro ont été comparées et leurs séquences déterminées. RecL a montré une augmentation significative du diamètre des plaques de lyse et une cinétique de réplication plus rapide que RecE. L'analyse moléculaire a montré plusieurs modifications de la séquence nucléotidique et en acides aminés entre RecE et les souches parentales ainsi qu’entre RecE et RecL. Ces résultats suggèrent que la recombinaison se produisant in vitro entre des souches de norovirus murins homologues peut générer une souche chimère, qui présente des propriétés biologiques similaires aux souches parentales et est capable d'une infection productive in vivo. De plus, ils montrent l'adaptation virale et la récupération de la réplication virale in vitro après un événement de recombinaison. [less ▲]

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See detailExperimental bluetongue virus superinfection in calves previously immunized with bluetongue virus serotype 8
Martinelle, Ludovic ULg; Dal Pozzo, Fabiana ULg; Sarradin, P et al

in Veterinary Research (2016), 47(1), 73

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See detailFirst Results in the Use of Bovine Ear Notch Tag for Bovine Viral Diarrhoea Virus Detection and Genetic Analysis
Quinet, C; Czaplicki, G; Dion, E et al

in PLoS ONE (2016), 11(10), 0164451

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See detailInfectivity of a recombinant murine norovirus (RecMNV) in Balb/cByJ mice
Mathijs, Elisabeth; de Oliveira-Filho, Edmilson; Dal Pozzo, Fabiana ULg et al

in Veterinary Microbiology (2016), 192

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See detailStakeholders’ perceptions, attitudes and practices towards risk prevention in the food chain
Lupo, C; Wilmart, O; Van Huffel, X et al

in Food Control (2016), 66

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See detailBovine noroviruses: A missing component of calf diarrhoea diagnosis
Di Felice, Elisabetta; Mauroy, Axel ULg; Dal Pozzo, Fabiana ULg et al

in Veterinary Journal (2016), 207

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See detailClinical sentinel surveillance of equine West Nile fever, Spain
Saegerman, Claude ULg; Alba-Casals, A; Garcia-Bocanegra, I et al

in Transboundary and Emerging Diseases (2016), 63(2), 184-193

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See detailEpidemiology and Diagnosis of Q Fever in Animals and Humans in the 21st Century
Mainil, Jacques ULg; Monseur, Christine ULg; Saegerman, Claude ULg et al

Scientific conference (2015, November 13)

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See detailOrbivirus screening on dried blood spots from captive oryx in United Arab Emirates stresses the importance of pre-import measures
Martinelle, Ludovic ULg; Haegeman, Andy; Lignereux, Louis et al

Poster (2015, September 01)

Objective: Following reintroduction and conservation programs of the Arabian oryx (Oryx leucoryx) and the scimitar horned oryx (SHO, Oryx dammah) in the United Arab Emirates (UAE), import of animals from ... [more ▼]

Objective: Following reintroduction and conservation programs of the Arabian oryx (Oryx leucoryx) and the scimitar horned oryx (SHO, Oryx dammah) in the United Arab Emirates (UAE), import of animals from wild game ranches in the United States of America (USA) is not uncommon. Bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) are orbiviruses that are the causative agents of bluetongue disease (BT) and epizootic hemorrhagic disease (EHD), respectively. BTV and EHDV are endemic in the UAE and the USA. Sheep and some wild ruminant species are usually severely affected by BT whereas EHD mostly affects wild animals and sometimes cattle. The objective of this study was to estimate the prevalence of these orbiviruses in Arabian and SHO from captive herds in the UAE using serology and molecular virology. Dry blood spot sampling for orbivirus screening is also discussed. Methods: A total of 175 SHO and 16 Arabian oryx were sampled. The latters were imported from Texas (USA) two weeks before sampling. All sampled animals belonged to captive herds spread over the Al Wathba area. For biosecurity reasons and to simplify blood storage, elutes from dried blood spot were used for serological and virological tests. Drops of about 80 µl of blood were dispensed on Whatman protein saver cards, and then allowed to dry in the dark at room temperature for 48 hours. Blood spots were punched out in paper discs with a 6 mm diameter punch and diluted in 250 µl PBS and Tween 20 0.05%. Eluted samples were incubated overnight at room temperature and then used immediately or stored at -80°C. To assess the most suitable ELISA kit to detect anti-BTV antibodies from the oryx discs, similar discs were prepared using blood issued from BTV seropositive and viremic as well as seronegative and non-viremic cattle. Elutes from discs with dried-blood from cattle were tested by BTV competitive ELISA (cELISA), sandwich ELISA (sELISA) and indirect ELISA (iELISA) and compared to cELISA performed directly on the serum of the same animals. iELISA on cattle paper discs gave the best correspondence with cELISA on cattle serum and was therefore used to test the oryx paper discs. Subsequently oryx paper discs were tested to detect antibodies against EHDV by cELISA. All the paper discs elutes from Arabian oryx and ELISA positive elutes from SHO were also tested by pan-BTV RTqPCR targeting a fragment of BTV segment 5 and detecting all BTV serotypes. Serotype specific end-point RT-PCR targeting a fragment of segment 2 of BTV2, BTV8, BTV10, BTV11, BTV13 and BTV17 were performed on pan-BTV positive samples. Results: Three out of 175 SHO and eight out of 16 Arabian oryx were found BTV seropositive by iELISA. None of the animals could be found seropositive against EHDV. BTV genome was detected in 1/3 seropositive SHO and in 5/16 of the Arabian oryx, amongst those 2/5 were seronegative. Overall Cq values were high (33-39). End point PCR failed to detect positive samples for any of the tested serotypes. Conclusion: BTV seroprevalence and RNA detection in SHO was very limited. By contrast BTV could be demonstrated in 5/16 imported Arabian oryx by molecular virology and in 8/16 by serology. The sampling was realized two weeks after the animals arrived in UAE and some oryx were viremic and seronegative, possibly suggesting a recent infection. Among the local SHO a low BTV seroprevalence was observed (3/175) and no animals were found positive to EHDV. This result was quite surprising because previous studies showed a higher BTV seroprevalence in domestic and wild ruminants of the Arabian Peninsula with wide local variations. In addition, dried blood spot testing has been demonstrated being a convenient and reliable method of sampling when storage conditions are hazardous. BTV serotypes could not be determined by end-point RT-PCR. At least 15 different BTV serotypes were reported in the USA and at least 10 in the Middle East, thus the oryx could be infected by a serotype not tested so far. Since RTqPCR positive values were high, the sensitivity of end-point RT-PCR might be insufficient to detect BTV out of eluted blood spots. Additional testing will be performed to identify the virus on the serotype level and therefore provide new insights to clarify the origin of the infection of the oryx. These results stress the need for pre-import risk assessment, precaution and implementation of biosecurity measures when considering translocation of wild ruminant species susceptible to BTV and EHDV. [less ▲]

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See detailLaboratory findings suggesting an association between BoHV-4 and bovine abortions in southern Belgium
Delooz, L; Czaplicki, G; Houtain, JY et al

Poster (2015)

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See detailThree Different Routes of Inoculation for Experimental Infection with Schmallenberg Virus in Sheep
Martinelle; Poskin, A; Dal Pozzo, Fabiana ULg et al

in Transboundary and Emerging Diseases (2015)

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See detailDose-dependent effect of experimental Schmallenberg virus infection in sheep
Poskin, A; Martinelle, Ludovic ULg; Mostin, L et al

in Veterinary Journal (2014), 201(3), 419-422

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See detailPreliminary survey on the impact of Schmallenberg virus on sheep flocks in south of Belgium
Saegerman, Claude ULg; Martinelle, Ludovic ULg; Dal Pozzo, Fabiana ULg et al

in Transboundary and Emerging Diseases (2014), 61(5), 469-472

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See detailField veterinary survey on clinical and economic impact of Schmallenberg virus in Belgium
Martinelle, Ludovic ULg; Dal Pozzo, Fabiana ULg; Gauthier, B et al

in Transboundary and Emerging Diseases (2014), 61(3), 285-288

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See detailECONOMIC IMPACT OF USING AN ANTIVIRAL IN THE CONTROL OF A FOOT-AND-MOUTH DISEASE EPIZOOTIC IN SOUTHERN BELGIUM
Dal Pozzo, Fabiana ULg; Humblet, Marie-France ULg; Vandeputte, Sébastien ULg et al

Poster (2013, October)

Foot-and-mouth disease virus (FMDV) is a highly contagious pathogen of cloven-hoofed mammals and one of the biggest concerns for veterinary authorities. The control measures to be applied in case of an ... [more ▼]

Foot-and-mouth disease virus (FMDV) is a highly contagious pathogen of cloven-hoofed mammals and one of the biggest concerns for veterinary authorities. The control measures to be applied in case of an outbreak vary in function of the disease-free or disease-enzootic status. Vaccination depends on the prior identification of the involved viral serotype and subtype, it confers an immunity limited to 6 months and it requires between 4 to 7 days to trigger the immune response (i.e. immunity-gap). The use of anti-FMD drugs has been discussed as an alternative or supplementary method to be used in previously FMD-free countries/zones. Such an antiviral treatment could protect against the viral dissemination to fill the gap between vaccination and the rise of a protective immunity. Apart from broad spectrum antiviral agents, such as ribavirin, specific anti-FMDV molecules have been identified in vitro, but none of them has been used in clinical studies involving ruminants or pigs. Next to the anti-FMDV activity, the absence of toxicity and the withdrawal period influencing the food safety, the cost of the treatment would be another important parameter influencing the potential use of an antiviral agent in the control of a FMD outbreak. The aim of this study was to assess the economic impact of using an antiviral in the control of a FMD epizootic in southern Belgium (Walloon Region). This work was based on the results of previous investigations concerning the epidemiological and economic data of a FMD outbreak in Southern Belgium. In the considered scenario, the epizootic was caused by the introduction of an infected cow (during the incubation time) in a beef cattle farm during winter. During the two weeks between the brood cow introduction and the official declaration of the outbreak, animal movements occurred between other beef cattle farms. The economic effects of the epidemic were evaluated taking into account the air-borne transmission of FMDV, the occurrence of animal movements (two scenarios were considered, with a minimum of 2 and a maximum of 17 movements), the presence of bovine and small ruminant farms, as well as pig farms in the protection and surveillance zones around the initial and secondary outbreaks. The wild fauna was not involved in the epidemic. In order to integrate in the above scenario the application of an antiviral agent in the control of the disease, it was assumed that the efficacy of the anti-FMDV drug was proven by reducing viral excretion in infected animals as well as by preventing the infection in animals at risk. Two hypothetical prices were used to introduce in the model the costs related to the administration of the antiviral drug (5€ and 10€ per dose). Furthermore, different strategies of control could be envisaged, such as the administration of the drug to both domestic ruminants and pigs, or depending on the epidemiological role of these species in the FMD transmission and their density in the territory, the administration of the drug to only one of them. Other scenarios could be characterized by the use of the antiviral in the control of the epizootic within the protection and surveillance zones or in only one of them. The costs associated with the use of antivirals in the different proposed scenarios are compared to the costs and socio-economic losses associated with the FMD outbreak and the implementation of control measures. [less ▲]

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See detailStudy of the virulence of serotypes 4 and 9 of African horse sickness virus in two mouse models
De la Grandière de Noronha Cotta, Maria Ana ULg; Zonta, William ULg; Mauroy, Axel ULg et al

Poster (2013, October)

African horse sickness (AHSV) is an infectious disease caused by a double stranded positive RNA virus which belongs to the family Reoviridae, genus Orbivirus. The virus has nine serotypes and is ... [more ▼]

African horse sickness (AHSV) is an infectious disease caused by a double stranded positive RNA virus which belongs to the family Reoviridae, genus Orbivirus. The virus has nine serotypes and is transmitted by a culicoides biting midge, principally Culicoides imicola. African horse sickness causes severe morbidity and mortality up to 95 % in horses with severe economic losses. The establishment of an experimental model is needed for the investigation of the pathogenesis of this infection. Two mouse models, interferon-α receptor knock-out mice (A129 KO or IFNAR -/-) and immunocompetent mice (A129 WT), were tested. The viruses used for mice inoculations belonged to the two serotypes which caused epidemics in Europe, serotypes 4 and 9. The virus was inoculated by subcutaneous (SC) route and/or by intra-nasal (IN) route. Whole blood samples were taken from each mouse at regular intervals. The organs (liver, spleen, kidney, lung and brain) were taken at the end of the experiment or when the most affected mice were euthanized. All these samples were tested by a qRT-PCR targeting AHSV genome segment 7. Both serotypes of AHSV were detected by qRT-PCR until three weeks post-infection in blood of IFNAR -/- mice and A129 WT mice infected by SC route. Serotype 4 shows a higher peak of viremia than serotype 9. The peak of viremia was measured between day 2 and day 4 post-infection. These results demonstrate the potential of the immunodeficient mouse model for both clinical and biological features. The setting up of this mouse model has developed a tool for efficient in vivo study to characterize the in vivo virulence of this virus, to monitor the evolution of viral populations during in vivo replication cycles and to test the competence or vectorial capacity of indigenous Culicoides. Research supported by the Belgium Federal Public Service, Health, Food Chain Safety and Environment. [less ▲]

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See detailStudy of the virulence of serotypes 4 and 9 of the Orbivirus African horse sickness virus in two mouse models
De la Grandière de Noronha Cotta, Maria Ana ULg; Zonta, William ULg; Mauroy, Axel ULg et al

Conference (2013, September 12)

African horse sickness (AHSV) is an infectious disease caused by a double stranded positive RNA virus which belongs to the family Reoviridae, genus Orbivirus. The virus has nine serotypes and is ... [more ▼]

African horse sickness (AHSV) is an infectious disease caused by a double stranded positive RNA virus which belongs to the family Reoviridae, genus Orbivirus. The virus has nine serotypes and is transmitted by a culicoides biting midge, principally Culicoides imicola. The establishment of an experimental model is needed for the investigation of the pathogenesis of this infection. Two mouse models, interferon-α receptor knock-out mice (A129 KO or IFNAR -/-) and immunocompetent mice (A129 WT), were tested. The viruses used for mice inoculations belonged to the two serotypes which caused epidemics in Europe, serotypes 4 and 9. The virus was inoculated by subcutaneous (SC) route and/or by intra-nasal (IN) route. Whole blood samples were taken from each mouse at regular intervals. The organs (liver, spleen, kidney, lung and brain) were taken at the end of the experiment or when the most affected mice were euthanized. All these samples were tested by a qRT-PCR targeting AHSV genome segment 7. Both serotypes of AHSV were detected by qRT-PCR until three weeks post-infection in blood of IFNAR -/- mice and A129 WT mice infected by SC route. Serotype 4 shows a higher peak of viremia than serotype 9. The peak of viremia was measured between day 2 and day 4 post-infection. These results demonstrate the potential of the immunodeficient mouse model for both clinical and biological features. The setting up of this mouse model has developed a tool for efficient in vivo study of AHSV. Research supported by the Belgium Federal Public Service, Health, Food Chain Safety and Environment. [less ▲]

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