References of "Bardiau, Marjorie"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailA 3-year long study of Staphylococcus aureus isolates from subclinical mastitis in three Azawak zebu herds at the Sahelian experimental farm of Toukounous, Niger
Issa Ibrahim, Abdoulkarim; Duprez, Jean-Noël ULg; Bada-alambdeji, Rianatou et al

in Tropical Animal Health and Production (in press)

bovine mastitis. The aim of the present work was to follow in three herds and during the 3 years the clonality of S. aureus isolated from California Mastitis Test (CMT)-positive cows at the experimental ... [more ▼]

bovine mastitis. The aim of the present work was to follow in three herds and during the 3 years the clonality of S. aureus isolated from California Mastitis Test (CMT)-positive cows at the experimental station of Toukounous (Niger) by (i) comparing their pulsed field gel electrophoresis (PFGE) fingerprints, (ii) identifying their virulotypes by PCR amplification and (iii) assessing the production of capsule and the formation of biofilm. The 88 S. aureus isolates belonged to 14 different pulsotypes, 3 of them being predominant: A (30 %), D (27 %), B (15 %). A and B pulsotypes had the highest profile similarity coefficient (94 %), while others had similarity coefficients under 60 %. Seventy-five S. aureus isolates were further studied for their virulotypes, capsular antigens and biofilm production. Most surface factor-, leukocidin- and haemolysin-, but not the enterotoxin-encoding genes were detected in the majority (>75 %) of the isolates and were evenly distributed between the A, B and D pulsotype isolates. The majority of the 72 S. aureus positive with the cap5H or cap8H PCR produced the CP5 (82 %) or the CP8 (88 %) capsular antigen, respectively. Biofilm production by the 57 icaA-positive isolates was strong for 8 isolates, moderate for 31 isolates but weak for 18 isolates, implying that the icaA gene may not be expressed in vitro by one third of the positive isolates. Similar to other studies, those results confirm that a restricted number of S. aureus clones circulate within the three herds at Toukounous and that their specific virulence-associated properties must still be further studied [less ▲]

Detailed reference viewed: 25 (7 ULg)
Full Text
Peer Reviewed
See detailBovine Staphylococcus aureus: Subtyping, evolution, and zoonotic transfer.
Boss, R.; Cosandey, A.; Luini, M. et al

in Journal of dairy science (2016), 99(1), 515-28

Staphylococcus aureus is globally one of the most important pathogens causing contagious mastitis in cattle. Previous studies using ribosomal spacer (RS)-PCR, however, demonstrated in Swiss cows that ... [more ▼]

Staphylococcus aureus is globally one of the most important pathogens causing contagious mastitis in cattle. Previous studies using ribosomal spacer (RS)-PCR, however, demonstrated in Swiss cows that Staph. aureus isolated from bovine intramammary infections are genetically heterogeneous, with Staph. aureus genotype B (GTB) and GTC being the most prominent genotypes. Furthermore, Staph. aureus GTB was found to be contagious, whereas Staph. aureus GTC and all the remaining genotypes were involved in individual cow disease. In addition to RS-PCR, other methods for subtyping Staph. aureus are known, including spa typing and multilocus sequence typing (MLST). They are based on sequencing the spa and various housekeeping genes, respectively. The aim of the present study was to compare the 3 analytic methods using 456 strains of Staph. aureus isolated from milk of bovine intramammary infections and bulk tanks obtained from 12 European countries. Furthermore, the phylogeny of animal Staph. aureus was inferred and the zoonotic transfer of Staph. aureus between cattle and humans was studied. The analyzed strains could be grouped into 6 genotypic clusters, with CLB, CLC, and CLR being the most prominent ones. Comparing the 3 subtyping methods, RS-PCR showed the highest resolution, followed by spa typing and MLST. We found associations among the methods but in many cases they were unsatisfactory except for CLB and CLC. Cluster CLB was positive for clonal complex (CC)8 in 99% of the cases and typically positive for t2953; it is the cattle-adapted form of CC8. Cluster CLC was always positive for tbl 2645 and typically positive for CC705. For CLR and the remaining subtypes, links among the 3 methods were generally poor. Bovine Staph. aureus is highly clonal and a few clones predominate. Animal Staph. aureus always evolve from human strains, such that every human strain may be the ancestor of a novel animal-adapted strain. The zoonotic transfer of IMI- and milk-associated strains of Staph. aureus between cattle and humans seems to be very limited and different hosts are not considered as a source for mutual, spontaneous infections. Spillover events, however, may happen. [less ▲]

Detailed reference viewed: 15 (3 ULg)
Full Text
Peer Reviewed
See detailStaphylococcus aureus genotype B and other genotypes isolated from cow milk in European countries.
Cosandey, A.; Boss, R.; Luini, M. et al

in Journal of dairy science (2016), 99(1), 529-40

Staphylococcus aureus is globally one of the most important pathogens causing contagious mastitis in cattle. Previous studies, however, have demonstrated in Swiss cows that Staph. aureus isolated from ... [more ▼]

Staphylococcus aureus is globally one of the most important pathogens causing contagious mastitis in cattle. Previous studies, however, have demonstrated in Swiss cows that Staph. aureus isolated from bovine intramammary infection is genetically heterogeneous, with Staph. aureus genotype B (GTB) and GTC being the most prominent genotypes. In addition, Staph. aureus GTB was found to be contagious, whereas Staph. aureus GTC and all the remaining genotypes were involved in individual cow disease. The aim of this study was to subtype strains of Staph. aureus isolated from bovine mastitic milk and bulk tank milk to obtain a unified view of the presence of bovine staphylococcal subtypes in 12 European countries. A total of 456 strains of Staph. aureus were subjected to different typing methods: ribosomal spacer PCR, detection of enterotoxin genes, and detection of gene polymorphisms (lukE, coa). Major genotypes with their variants were combined into genotypic clusters (CL). This study revealed 5 major CL representing 76% of all strains and comprised CLB, CLC, CLF, CLI, and CLR. The clusters were characterized by the same genetic properties as the Swiss isolates, demonstrating high clonality of bovine Staph. aureus. Interestingly, CLB was situated in central Europe whereas the other CL were widely disseminated. The remaining 24% of the strains comprised 41 genotypes and variants, some of which (GTAM, GTBG) were restricted to certain countries; many others, however, were observed only once. [less ▲]

Detailed reference viewed: 20 (7 ULg)
Full Text
Peer Reviewed
See detailThe antibiotic resistance in the environment
Bardiau, Marjorie ULg

Conference (2015, October)

Detailed reference viewed: 10 (0 ULg)
Full Text
Peer Reviewed
See detailRemoval of antibiotic resistant bacteria in wastewater treatment plant
Bardiau, Marjorie ULg; Trajano, D; Taylor, H et al

Poster (2015, June)

Detailed reference viewed: 13 (0 ULg)
Full Text
Peer Reviewed
See detailEfficacy of Antisepsis Measures during Manual Milking on the Prevalence of Bacterial Mastitis at the Sahelian Experimental Station of Toukounous (Niger).
Issa Ibrahim, Abdoulkarim ULg; Bada-Alambedji, Rianatou; Duprez, Jean-Noël ULg et al

in African Journal of Microbiology Research (2015), 9(6), 348-354

Detailed reference viewed: 41 (14 ULg)
Full Text
Peer Reviewed
See detailAntibiotic resistance trend of Staphylococcus aureus isolated between 2010 and 2012 from mastitis cases in Azawak zebu in Niger
Issa Ibrahim, Abdoulkarim ULg; Duprez, Jean-Noël ULg; Bada-Alambedji, Rianatou et al

in African Journal of Microbiology Research (2014), 8((35)), 3271-3275

Detailed reference viewed: 31 (10 ULg)
Full Text
Peer Reviewed
See detailMolecular evidence of Anaplasma phagocytophilum in wild boar (Sus scrofa) in Belgium
Nahayo, Adrien; Bardiau, Marjorie ULg; Volpe, Rosario ULg et al

in BMC Veterinary Research (2014), 10(80),

Anaplasma phagocytophilum is a tick-borne pathogen of veterinary and human importance. Both ticks as vectors and vertebrates as reservoir hosts are essential for the cycle maintenance of this bacterium ... [more ▼]

Anaplasma phagocytophilum is a tick-borne pathogen of veterinary and human importance. Both ticks as vectors and vertebrates as reservoir hosts are essential for the cycle maintenance of this bacterium. Currently, the whole range of animal species reservoirs for A. phagocytophilum in natural environment is still unknown. Therefore, the aim of this study was to estimate the prevalence of infection with A. phagocytophilum in the wild boar population in southern Belgium. [less ▲]

Detailed reference viewed: 40 (7 ULg)
Full Text
Peer Reviewed
See detailBacterial mastitis in the Azawak zebu breed at the Sahelian experimental station in Toukounous (Niger): Identification and typing of Staphylococcus aureus
Issa Ibrahim, Abdoulkarim; Bada-Alambedji, Rlanatou; Duprez, Jean-Noel et al

in International Research Journal of Microbiology [=IRJM] (2013), 4(7), 168-178

Detailed reference viewed: 49 (21 ULg)
Full Text
Peer Reviewed
See detailGenotypic and phenotypic characterisation of methicillin-resistant Staphylococcus aureus (MRSA) isolated from milk of bovine mastitis
Bardiau, Marjorie ULg; Yamazaki, Kazuko; Duprez, Jean-Noel et al

in Letters in Applied Microbiology (2013)

Detailed reference viewed: 31 (6 ULg)
Full Text
Peer Reviewed
See detailCharacterization of methicillin-resistant Staphylococcus pseudintermedius isolated from dogs and cats
Bardiau, Marjorie ULg; Yamazaki, Kazuko; Ote, Isabelle et al

in Microbiology and Immunology (2013)

Detailed reference viewed: 33 (6 ULg)
Full Text
See detailMRSA: le point de vue du bactériologiste
Bardiau, Marjorie ULg

Conference (2013, January)

Detailed reference viewed: 36 (6 ULg)