References of "Amory, Hélène"
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See detailL'échocardiographie en médecine équine: principe et interprétation des images.
Amory, Hélène ULg

in Nouveau Praticien Vétérinaire Equine (2016), 11(39), 55-60

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See detailMitochondrial function is altered in horse atypical myopathy
Lemieux, Hélène; BOEMER, François ULg; van Galen, Gaby et al

in Mitochondrion (2016), 30

Equine atypical myopathy in Europe is a fatal rhabdomyolysis syndrome that results from the ingestion of hypoglycin A contained in seeds and seedlings of Acer pseudoplatanus. The hallmark of atypical ... [more ▼]

Equine atypical myopathy in Europe is a fatal rhabdomyolysis syndrome that results from the ingestion of hypoglycin A contained in seeds and seedlings of Acer pseudoplatanus. The hallmark of atypical myopathy consists of a severe alteration in the energy metabolism including a severe impairment in muscle mitochondrial respiration that could contribute to its high death rate [less ▲]

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See detailFaecal microbiota characterisation of horses using 16 rdna barcoded pyrosequencing, and carriage rate of clostridium difficile at hospital admission
Rodriguez Diaz, Cristina ULg; Taminiau, Bernard ULg; Brévers, Bastien et al

in BMC Microbiology (2015), 15

Background The equine faecal microbiota is very complex and remains largely unknown, while interspecies interactions have an important contribution to animal health. Clostridium difficile has been ... [more ▼]

Background The equine faecal microbiota is very complex and remains largely unknown, while interspecies interactions have an important contribution to animal health. Clostridium difficile has been identified as an important cause of diarrhoea in horses. This study provides further information on the nature of the bacterial communities present in horses developing an episode of diarrhoea. The prevalence of C. difficile in hospitalised horses at the time of admission is also reported. Results Bacterial diversity of the gut microbiota in diarrhoea is lower than that in non-diarrhoeic horses in terms of species richness (p-value <0.002) and in population evenness (p-value: 0.02). Statistical differences for Actinobacillus, Porphyromonas, RC9 group, Roseburia and Ruminococcaceae were revealed. Fusobacteria was found in horses with diarrhoea but not in any of the horses with non-diarrheic faeces. In contrast, Akkermansia was among the three predominant taxa in all of the horses studied. The overall prevalence of C. difficile in the total samples of hospitalised horses at admission was 3.7 % (5/134), with five different PCR-ribotypes identified, including PCR-ribotype 014. Two colonised horses displayed a decreased bacterial species richness compared to the remaining subjects studied, which shared the same Bacteroides genus. However, none of the positive animals had diarrhoea at the moment of sampling. Conclusions The abundance of some taxa in the faecal microbiota of diarrhoeic horses can be a result of microbiome dysbiosis, and therefore a cause of intestinal disease, or some of these taxa may act as equine enteric pathogens. Clostridium difficile colonisation seems to be transient in all of the horses studied, without overgrowth to trigger infection. A large proportion of the sequences were unclassified, showing the complexity of horses’ faecal microbiota. [less ▲]

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See detailTWO-DIMENSIONAL SPECKLE TRACKING ECHOCARDIOGRAPHY IN CALVES: FEASIBILITY, REPEATABILITY AND VARIABILITY STUDY
Lecoq, Laureline ULg; Amory, Hélène ULg; Leroux, Aurélia ULg

in Journal of Veterinary Internal Medicine (2015), 29(4), 1255

Two-dimensional speckle tracking (2DST) is a non-invasive technique used in many species to evaluate global and regional left ventricular (LV) function; however it received little attention in the bovine ... [more ▼]

Two-dimensional speckle tracking (2DST) is a non-invasive technique used in many species to evaluate global and regional left ventricular (LV) function; however it received little attention in the bovine species. The aim of this study was to assess the feasibility and reliability of 2DST for the evaluation of circumferential and radial LV wall motions in calves. Fourteen Holstein black calves (age: 62 11.6 days; body weight: 75.25 5.4 kg) were used in this observational study. Right parasternal short axis views at the level of the papillary muscles were recorded and subsequently analysed by 2DST for global and regional radial and circumferential strains and strain rates, radial displacement, rotation and rotation rate. Echocardiographic examinations were performed in unsedated, standing calves by two different observers to evaluate intra- and interobserver repeatability and variability. 2DST was feasible in all calves but 2 were excluded from analyses (ventricular septum defect and resting heart rate above 120 bpm, respectively). Automated tracking was better in systole than in diastole. Intraobserver repeatability was good to moderate for most systolic global and segmental peak values. Systolic peak values for radial strain and strain rate were more repeatable than for circumferential strain, circumferential train rate and diastolic measurements. Variability of the interobserver measurements was greater than the intraobserver measurements. Two-dimensional speckle tracking is feasible in calves and as in other species systolic radial function can be more reliably evaluated than circumferential and diastolic function. [less ▲]

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See detailQuantification of hypoglycin A in serum using aTRAQ((R)) assay.
BOEMER, François ULg; DEBERG, Michelle ULg; SCHOOS, Roland ULg et al

in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2015), 997

BACKGROUND: Hypoglycin A has been recently identified has the causal agent of atypical myopathy (AM) in horses. Its identification and quantification in equine's biological fluids is thus a major concern ... [more ▼]

BACKGROUND: Hypoglycin A has been recently identified has the causal agent of atypical myopathy (AM) in horses. Its identification and quantification in equine's biological fluids is thus a major concern to confirm maple poisoning and to provide insight into the poorly understood mechanism of hypoglycin A intoxication. METHODS: Quantification of hypoglycin A has been achieved with the aTRAQ kit for amino acid analysis of physiological fluids (AB Sciex). Acquisition method on mass spectrometer has been updated to record the hypoglycin A specific MRM transition. RESULTS: Outlined accuracy profiles demonstrated very reliable data. A good linearity was observed from 0.09 to 50mumol/L and precision was very good with coefficient of variation below 8%. Fifty-five samples collected from 25 confirmed AM horses revealed significant hypoglycin A concentrations, while toxin was not found in serum of 8 control animals. CONCLUSIONS: The described aTRAQ variant method has been analytically and clinically validated. The reliability of our approach is thus demonstrated into the workup of atypical myopathy. [less ▲]

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See detailLes nouveaux outils de diagnostic et de pronostic de la myopathie atypique
Habyarimana, Jean ULg; BOEMER, François ULg; Amory, Hélène ULg et al

in Proceeding de la 41ème Journée de la Recherche équine (2015)

In equines, ingestion of hypoglycin A, a toxin produced in the seeds (samaras) of sycamore maple (Acer pseudoplatanus) tree alters the energetic metabolism of muscle cells and results in atypical myopathy ... [more ▼]

In equines, ingestion of hypoglycin A, a toxin produced in the seeds (samaras) of sycamore maple (Acer pseudoplatanus) tree alters the energetic metabolism of muscle cells and results in atypical myopathy (AM). This alterations leads to a characteristic biochemical profile of acylcarnitines (AC) that enables to confirm the diagnosis of AM. This study aims at validating a methodology for the dosage of hypoglycin A in vegetal extracts but also in blood. In addition, the biochemical profile in AC has been determined in AM cases (5 survivors and 13 deceased) and in 5 horses suffering from exercise-induced myopathy. The AC profiles of these horses have been compared to the one of healthy horses (n = 35). This study showed that hypoglycin A was present in seeds and spring seedlings of sycamore and also in blood of AM cases horses. In addition, the establishment of AC profile contributes to the diagnostic and helps to assess the prognosis of AM cases. [less ▲]

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See detailBorréliose: mythe ou réalité ?
Amory, Hélène ULg; Houben, Rosa ULg; Pitel, Pierre Hugues et al

Conference (2014, November 15)

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See detailAtrial Natriuretic Peptide in cardiac horses
Leroux, Aurélia ULg; Al-Haidar, Ali; Remy, Benoît ULg et al

in Voorjaarsdagen Proceedings (2014, April 17)

Background: Natriuretic peptides are cardiac biomarkers that have been shown to have high potential for diagnosis, prognosis, and guidance of treatment in human and small animal cardiology. However, their ... [more ▼]

Background: Natriuretic peptides are cardiac biomarkers that have been shown to have high potential for diagnosis, prognosis, and guidance of treatment in human and small animal cardiology. However, their diagnostic and prognosis value in the equine species has only been studied in a limited number of cases. Objective: To investigate the plasma atrial natriuretic peptide concentration (ANPPl) in a large group of horses with various degrees of valvular regurgitation (VR) and heart failure (HF). Animals: Ninety-one horses, admitted at the equine teaching hospital of the University of Liege, and with no, mild, moderate or severe VR and presenting various stages of HF, according to human and canine grading system (A: no VR, B1: asymptomatic VR without cardiac remodelling, B2: asymptomatic VR with cardiac remodelling, C: symptomatic VR). Methods: All horses underwent clinical and Doppler echocardiographic examination, which allowed determining the presence, nature, and severity of VR, and the HF stage. The ANPPl was measured using a commercially available human RIA test. The mean values of body weight, age, ANPPl and each echocardiographic parameter were compared between horses in stage A, B1, B2 or C of HF and between horses with no, mild, moderate or severe VR, using a one-way ANOVA test. Then, correlations between ANPPl and each echocardiographic parameter were assessed using a Pearson’s product-moment analysis. Results: Horses with severe and moderate VR had significantly higher ANPPl than horses with mild or no VR. ANPPl was significantly higher in horses at stage B2 and C of HF than horses at stage A and B1. Moreover, ANPPl was significantly correlated with most of the measured echocardiographic variables. This correlation was especially strong with the left atrial diameter and its percentage of dilation. Conclusions and Clinical Importance: Those results suggest that ANPPl in horses suffering from VR could have a high diagnostic value of HF, especially in horses with tricuspid or mitral insufficiency and with enlargement or dysfunction of the atria. [less ▲]

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See detailPrognostic value of echocardiographic and Doppler parameters in horses admitted for colic complicated by systemic inflammatory response syndrome
Borde, L.; Amory, Hélène ULg; Grulke, Sigrid ULg et al

in Journal of Veterinary Emergency and Critical Care (2014), 24(3), 302-310

Objective: To assess the prognostic value of echocardiographic parameters of left ventricular (LV) function in horses with systemic inflammatory response syndrome (SIRS). Design: Prospective observational ... [more ▼]

Objective: To assess the prognostic value of echocardiographic parameters of left ventricular (LV) function in horses with systemic inflammatory response syndrome (SIRS). Design: Prospective observational study. Setting: Veterinary teaching hospital. Animals: Forty-one horses admitted for colic with clinical evidence of SIRS. Interventions: All horses underwent Doppler echocardiographic examination on admission. LV echocardiographic parameters, including pulsed-wave tissue Doppler imaging parameters, were compared between nonsurvivors (n = 29) and horses that survived to discharge (n = 12). Measurements and Main Results: With comparable heart rate and LV preload estimate, LV stroke volume index, the velocity time integral, deceleration time, ejection time of Doppler aortic flow, and peak early diastolic myocardial velocity were lower in the nonsurviving than in the surviving horses, while pre-ejection period to ejection time ratio (PEP/ET) of Doppler aortic flow and the peak early diastolic filling velocity to peak early diastolic myocardial velocity ratio (E/Em) were higher (P < 0.05). A cut-off value of 0.26 for PEP/ET predicted mortality with 100% sensitivity and 42% specificity (area under the receiver operating characteristic curve: 0.71), whereas a cut-off value of 2.67 for E/Em predicted mortality with 100% sensitivity and 83% specificity (area under the receiver operating characteristic curve: 0.89). Conclusions: Echocardiography may provide prognostic information in colic horses with clinical evidence of SIRS. Especially, PEP/ET and E/Em could be useful markers of systolic and diastolic dysfunction, respectively, to detect horses with a high risk of death requiring more intensive cardiovascular monitoring as it has been reported in human patients with septic shock. © Veterinary Emergency and Critical Care Society 2014. [less ▲]

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See detailLe syndrome de fièvre isolée ou piro-like.
Amory, Hélène ULg; Pitel, Pierre-Hugues

in Pratique Vétérinaire Equine (2014), 46

Le diagnostic étiologique en cas de syndrome fébrile chez le cheval est particulièrement difficile, sauf s’il est accompagné de signes spécifiques. Face à un cas, après avoir réalisé une anamnèse et un ... [more ▼]

Le diagnostic étiologique en cas de syndrome fébrile chez le cheval est particulièrement difficile, sauf s’il est accompagné de signes spécifiques. Face à un cas, après avoir réalisé une anamnèse et un examen clinique approfondis, le vétérinaire devra dans la plupart des cas mettre en place une batterie d’examens complémentaires qui peuvent s’avérer onéreux. Il devra donc procéder en tenant compte du moindre élément du tableau clinique ou des examens complémentaires de base qui peuvent s’avérer fort utiles pour hiérarchiser les examens à mettre en place. Parmi les maladies infectieuses que le vétérinaire gardera à l’esprit sur un cas de fièvre isolée, sans atteinte claire d’un système particulier et sur lequel les autres hypothèses de diagnostic ont été écartées, que les maladies incluses dans le syndrome piro-like, à savoir la piroplasmose, la borréliose, la leptospirose, l’anaplasmose et l’anémie infectieuse équine, constituent des hypothèses à vérifier. [less ▲]

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See detailStéatite généralisée chez 3 chevaux adultes référés à l’université de Liège
Leroux, Aurélia ULg; Garcia da Fonseca, Rita; Bayrou, Calixte ULg et al

in Pratique Vétérinaire Equine (2014), 184

In horses, two forms of generalised steatitis have been described: one subcutaneous nodular form and one diffuse systemic form. Three cases of the second form are described in this paper. The clinical ... [more ▼]

In horses, two forms of generalised steatitis have been described: one subcutaneous nodular form and one diffuse systemic form. Three cases of the second form are described in this paper. The clinical signs included depression, anorexia, diarrhoea, abdominal pain, fever, subcutaneous oedema, tachycardia and tachypnea. Blood analyses showed anemia, neutrophilia, hypoalbuminemia, increased muscular enzymes activities, and low level of vitamine E concentration. Abdominal ultrasound demonstrated an irregular hyperechoic band of peritoneal fat tissue. Ante mortem diagnosis was achieved in 2 horses, one on the basis of rectal biopsies and one on the basis of biopsies of the nuchal ligament. Despite a corticoids therapy and vitamin E supplementation, the 3 horses died or were euthanized. Those cases confirm that, in horses, diagnosis of systemic diffuse generalised steatitis is difficult and that the prognosis is poor. [less ▲]

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See detailAtrial Natriuretic Peptide as an indicator of the severity of valvular regurgitation and heart failure in horses
Leroux, Aurélia ULg; Al Haidar, Ali; Remy, Benoît ULg et al

in Journal of Equine Veterinary Science (2014), 34(10), 1226-1233

Natriuretic peptides are cardiac biomarkers, routinely used for diagnosis, prognosis, and guidance for treatment in human and small animal cardiology. However, their diagnostic and prognostic value in ... [more ▼]

Natriuretic peptides are cardiac biomarkers, routinely used for diagnosis, prognosis, and guidance for treatment in human and small animal cardiology. However, their diagnostic and prognostic value in horses has received little study. The aim of this study was to investigate the plasma atrial natriuretic concentration (ANPPl) in a large group of horses with various degrees of valvular regurgitation (VR) and congestive heart failure (CHF). Clinical examination and two-dimensional, time-motion mode and Doppler echocardiography were performed on 91 horses admitted to the Equine Teaching Hospital of Liege University, with either no, mild, moderate or severe VR and presenting various stages of CHF. ANPPl was measured using a commercially available human RIA test. Mean values of body weight, age, ANPPl and echocardiographic parameters were compared between horses with no, mild, moderate and severe VR and between horses in different CHF stages. Correlation and linear regression of ANPPl with each echocardiographic parameter and with the percentage of dilation of each of cardiac chambers were assessed. Horses with severe or moderate VR had significantly higher ANPPl than horses with mild or no VR. ANPPl was significantly higher in horses presenting CHF than in horses without CHF. ANPPl was significantly correlated with the left atrial diameter and its percentage of dilation. These results suggest a diagnostic value of ANPPl in horses with VR, especially with tricuspid or mitral insufficiency with dilation of the atria. [less ▲]

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See detailCarriage and acquisition rates of Clostridium difficile in hospitalized horses, including molecular characterization, multilocus sequence typing and antimicrobial susceptibility of bacterial isolates
Rodriguez Diaz, Cristina ULg; Taminiau, Bernard ULg; Brévers, Bastien et al

in Veterinary Microbiology (2014)

lostridium difficile has been identified as a significant agent of diarrhoea and enterocolitis in both foals and adult horses. Hospitalization, antibiotic therapy or changes in diet may contribute to the ... [more ▼]

lostridium difficile has been identified as a significant agent of diarrhoea and enterocolitis in both foals and adult horses. Hospitalization, antibiotic therapy or changes in diet may contribute to the development of C. difficile infection. Horses admitted to a care unit are therefore at greater risk of being colonized. The aim of this study was to investigate the carriage of C. difficile in hospitalized horses and the possible influence of some risk factors in colonization. During a seven-month period, faecal samples and data relating the clinical history of horses admitted to a veterinary teaching hospital were collected. C. difficile isolates were characterized through toxin profiles, cytotoxicity activity, PCR-ribotyping, antimicrobial resistance and multilocus sequence typing (MLST). Ten isolates were obtained with a total of seven different PCR-ribotypes, including PCR-ribotype 014. Five of them were identified as toxinogenic. A high resistance to gentamicin, clindamycin and ceftiofur was found. MLST revealed four different sequencing types (ST), which included ST11, ST26, ST2 and ST15, and phylogenetic analysis showed that most of the isolates clustered in the same lineage. Clinical history suggests that horses frequently harbour toxigenic and non-toxigenic C. difficile and that in most cases they are colonized regardless of the reason for hospitalization; the development of diarrhoea is more unusual. [less ▲]

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See detailÉtiopathogénie de la myopathie atypique et gestion raisonnée des cas
Votion, Dominique ULg; Patarin, Florence; Cerri, Simona ULg et al

in In Proceedings: Journée AVEF (Association des Vétérinaires Equins Français) (2013, December)

Atypical myopathy is an acute intoxication resulting from the ingestion of a toxin contained in the seeds of some trees of the genus Acer (i.e. « maple trees »). The causes of the emergence of the disease ... [more ▼]

Atypical myopathy is an acute intoxication resulting from the ingestion of a toxin contained in the seeds of some trees of the genus Acer (i.e. « maple trees »). The causes of the emergence of the disease due to trees naturalized for many decades remain undetermined. Currently, there is no cure for atypical myopathy but symptomatic treatment may be revisited on the basis of knowledge of the aetio-pathogenesis of the disease. This paper compiles recommendations following a discussion on treatment of atypical myopathy held in October 2013 at the University of Liege (Belgium) to provide emergency treatment distinguishing clinical management (1) at hospital, (2) in the field and (3) preventive measures to protect pasture companions. [less ▲]

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See detailPrevalence and Risk Factors for Various Cardiac Diseases in a Hospital-Based Population of 3434 Horses (1994-2011)
Leroux, Aurélia ULg; Detilleux, Johann ULg; Sandersen, Charlotte ULg et al

in Journal of Veterinary Internal Medicine (2013), 27(6), 1563-70

Background: Risk factors for several cardiac diseases have been suspected in horses, but few have been statistically demonstrated in a large equine population. Objectives: To describe risk factors for ... [more ▼]

Background: Risk factors for several cardiac diseases have been suspected in horses, but few have been statistically demonstrated in a large equine population. Objectives: To describe risk factors for various cardiac diseases in a hospital-based equine population. Animals: Files of 3434 horses admitted at the internal medicine department of the Liege Equine Teaching Hospital between 1994 and 2011were reviewed and of those, 284 were categorized as suffering from moderate to severe cardiac disease. Methods: Observational study. After calculating prevalence for each cardiac disease, we tested whether breed (chi-square test) or gender, age, body weight (BW) and other cardiac diseases (logistic regressions) were risk factors (p<0.05 significant). Results: Mitral regurgitation (MR, 4.4%), atrial fibrillation (AF, 2.3%), aortic regurgitation (AR, 2.1%) and tricuspid regurgitation (TR, 1.7%) were the most common cardiac abnormalities. Determinants were male gender and increasing age for AR (OR=2.03, CI=1.07-4.94), racehorses breed and middle-age for TR (OR=4.36; CI=1.10-17.24), and high BW for AF (OR=3.54; CI=1.67-7.49). MR was the major valvular disease associated with AF, pathological ventricular arrhythmia, pulmonary regurgitation (PR) and congestive heart failure (CHF). TR was also linked to AF, PR and CHF; AR was not linked to CHF. Conclusions and clinical importance: Several previously suspected risks factors of a variety of equine cardiac diseases are statistically confirmed in the studied hospital-based population. Similar observation could be suspected in a larger randomized population and should be taken into account in health and sport’s monitoring of horses presenting predisposing factors. [less ▲]

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See detailL’insuffisance mitrale chez le cheval : revue de la littérature et comparaison avec la pathologie chez l’homme et chez le chien
Al Haidar, Ali; Leroux, Aurélia ULg; Amory, Hélène ULg

in Annales de Médecine Vétérinaire (2013), 157(1), 52-68

Abstract Mitral regurgitation (MR) is a one of the most common cardiac disease in the equine species. Furthermore, it is the valvular disease that presents the higher risk to be associated with reduced ... [more ▼]

Abstract Mitral regurgitation (MR) is a one of the most common cardiac disease in the equine species. Furthermore, it is the valvular disease that presents the higher risk to be associated with reduced athletic performance, to evolve into congestive heart failure and to become life threatening. The clinical diagnosis of MR depends mainly on the expertise of the veterinarian in cardiology and is above all based on cardiac auscultation. In all of the cases, the definitive diagnosis and the evaluation of the disease severity require performing a Doppler echocardiographic examination. Signs of left ventricular and/or left atrial overload, large regurgitation jet, pulmonary arterial hypertension or significant arrhythmias are negative prognosis factors. Clinical signs of congestive heart failure make the prognosis worse. Other echocardiographic parameters are used in human and dogs in order to determine the severity of the MR, but have been not validated in horses. Treatment of mitral regurgitation in the equine species is symptomatic and directed against the development of a congestive heart failure. A lot of drugs that are used in human or dogs are of limited value in horses for financial reasons and/or lack of pharmacokinetics and pharmacologic data in equine species. [less ▲]

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See detailCan horses be clinically screened for West Nile fever ?
van galen; Calozet, L; Leblond, Agnès et al

in Veterinary Record : Journal of the British Veterinary Association (2013), 172(4), 101

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See detailEvaluation of acepromazine-induced hemodynamic alterations and reversal with norepinephrine infusion in standing horses
Pequito, Manuel; Amory, Hélène ULg; De Moffarts, Brieux et al

in Canadian Veterinary Journal = Revue Vétérinaire Canadienne (2013), 54

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