Production d’arômes de type lactone par des levures.

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14(4), 681-691

The use of Macronet resins to recover decalactone produced by Rhodotorula aurantiaca from the culture broth

in Journal of Industrial Microbiology & Biotechnology (2010), 37(2), 167-172

During the biotransformation of castor oil into -decalactone, R. aurantiaca produced both the lactone form and its precursor (4-hydroxydecanoic acid). After six days of culture, a maximum yield of ... [more ▼]

During the biotransformation of castor oil into -decalactone, R. aurantiaca produced both the lactone form and its precursor (4-hydroxydecanoic acid). After six days of culture, a maximum yield of -decalactone of 6.5 g/l was obtained. The parameters of -decalactone adsorption on three Macronet resins (MN-202, MN-102 and MN-100) were investigated in water. Adsorption isotherms of -decalactone for the three Macronet resins were linear. The trapping of -decalactone produced by R. aurantiaca on these resins was then carried out. -Decalactone was eVectively retained by all the studied Macronet resins. The resin MN-202 trapped -decalactone more eYciently than MN-102 and MN-100. The percentages of -decalactone adsorbed on the resins MN-202, MN-102 and MN-100 were, respectively, 85, 75 and 81%, whereas around 70% of the adsorbed -decalactone was then desorbed. We propose an industrial process that uses Macronet resins to extract -decalactone from culture broth of R. aurantiaca. [less ▲]

Production of γ-Decalactone by a Psychrophilic and a Mesophilic Strain of the Yeast

in Applied Biochemistry and Biotechnology (2009)

Among 18 psychrophilic strains isolated near the Antarctic Station, the psychrophilic strain Rhodotorula aurantiaca A19 was selected for its ability of growth and γ- decalactone production at low ... [more ▼]

Among 18 psychrophilic strains isolated near the Antarctic Station, the psychrophilic strain Rhodotorula aurantiaca A19 was selected for its ability of growth and γ- decalactone production at low temperatures. The effects of temperature, initial pH, and castor oil concentration on the growth and γ-decalactone production by a psychrophilic and a mesophilic strain of R. aurantiaca were investigated. The highest γ-decalactone production in flasks (5.8 g/l) was obtained with the strain A19 at 14 °C and initial pH 7.0 in medium containing 20 g/l castor oil. On the other hand, these factors did not affect the production of γ-decalactone by the mesophilic strain. In fermentor, a γ-decalactone concentration of 6.6 g/l was reached with the strain A19, whereas a maximum of 0.1 g/l was obtained with the mesophilic strain. Our results suggest that the ability to synthesize γ-decalactone is a particularity of the strain A19, since the mesophilic strain (no. 30645) produced small amounts, and the other (no. 31354) did not exhibit this property. It is, to our knowledge, the first report of γ-decalactone production by R. aurantiaca and furthermore by a psychrophilic yeast strain. Moreover, the amount of γ-decalactone obtained in fermentor with the strain 19 was on the order of concentrations usually described in patents. [less ▲]

Production and toxicity of γ-decalactone and 4-hydroxydecanoic acid from Rhodotorula aurantiaca

Poster (2009)

The objective of this study was to compare the production of γ- decalactone in different scales and to study the effects of 4- hydroxydecanoic acid and γ-decalactone on the growth of R. aurantiaca. The ... [more ▼]

The objective of this study was to compare the production of γ- decalactone in different scales and to study the effects of 4- hydroxydecanoic acid and γ-decalactone on the growth of R. aurantiaca. The effect of gum tragacanth on the production of γ-decalactone by R. aurantiaca was evaluated. [less ▲]