  Quantification of tagitinin C in Tithonia diversifolia by reversed-phase high-performance liquid chromatography.Goffin, Eric  ; ; Ziemons, Eric et alin Phytochemical Analysis [=PCA] (2003), 14(6), 378-80 A simple, rapid and reliable reversed-phase high-performance liquid chromatographic method for the determination of tagitinin C, an anti-plasmodial sesquiterpene lactone isolated from the aerial parts of ... [more ▼] A simple, rapid and reliable reversed-phase high-performance liquid chromatographic method for the determination of tagitinin C, an anti-plasmodial sesquiterpene lactone isolated from the aerial parts of Tithonia diversifolia, has been developed. The assay has been used to quantify tagitinin C in various extracts of the aerial parts of T. diversifolia. [less ▲] Detailed reference viewed: 65 (12 ULg) In vitro antiplasmodial activity of Tithonia diversifolia and identification of its main active constituent: tagitinin C.Goffin, Eric ; Ziemons, Eric ; De Mol, Patrick et alin Planta Medica (2002), 68(6), 543-5 The antimalarial properties of Tithonia diversifolia, an Asteraceae traditionally used to treat malaria, were investigated in vitro against three strains of Plasmodium falciparum. The ether extract from ... [more ▼] The antimalarial properties of Tithonia diversifolia, an Asteraceae traditionally used to treat malaria, were investigated in vitro against three strains of Plasmodium falciparum. The ether extract from aerial parts of the plant collected in Sao Tome e Principe, demonstrated good antiplasmodial activity (IC 50 on FCA strain: 0.75 microg/ml). A bioassay guided fractionation of this extract led to the isolation of the known sesquiterpene lactone tagitinin C as an active component against Plasmodium (IC 50 on FCA strain: 0.33 microg/ml), but also possessing cytotoxic properties (IC 50 on HTC-116 cells: 0.706 microg/ml). [less ▲] Detailed reference viewed: 116 (10 ULg) |
||
