References of "Zorzi, Willy"
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See detailDevelopment of a quantitative immunocapture real-time PCR assay for detecting structurally intact adenoviral particles in water
Ogorzaly, Leslie; Bonot, Sébastien; Elmoualij, Benaïssa ULg et al

in Journal of Virological Methods (2013), 194

Development of rapid, sensitive and specific methods for detection of infectious enteric viruses in water is challenging but crucial for gaining reliable information for risk assessment. An immunocapture ... [more ▼]

Development of rapid, sensitive and specific methods for detection of infectious enteric viruses in water is challenging but crucial for gaining reliable information for risk assessment. An immunocapture real-time PCR (IC-qPCR) was designed to detect jointly the two major viral particle components, i.e. the capsid protein and the viral genome. Targeting both constituents helps circumventing the technical limits of cell culture approaches and the inability of PCR based methods to predict the infectious status. Two waterborne pathogenic virus models, human adenovirus types 2 and 41, were chosen for this study. IC-qPCR showed a detection limit of 10 MPNCU/reaction with a dynamic range from 102 to 106 MPNCU/reaction. Sensitivity was thus 100-fold higher compared to ELISA-based capture employing the same anti-hexon antibodies. After optimisation, application on environmental water samples was validated, and specificity towards the targeted virus types was obtained through the qPCR step. Heat-treated pure samples as well as surface water samples brought evidence that this method achieves detection of encapsidated viral genomes while excluding free viral genome amplification. As a consequence, adenovirus concentrations estimated by IC-qPCR were below those calculated by direct qPCR. The results demonstrate that the IC-qPCR method is a sensitive and rapid tool for detecting, in a single-tube assay, structurally intact and thus potentially infectious viral particles in environmental samples. [less ▲]

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See detailA New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Wel
Mille, Caroline; Debarnot, Dominique; Zorzi, Willy ULg et al

in Biosensors (2012), 2(4), 433-447

This work illustrates the enhancement of the sensitivity of the ELISA titration for recombinant human and native prion proteins, while reducing other non-specific adsorptions that could increase the ... [more ▼]

This work illustrates the enhancement of the sensitivity of the ELISA titration for recombinant human and native prion proteins, while reducing other non-specific adsorptions that could increase the background signal and lead to a low sensitivity and false positives. It is achieved thanks to the association of plasma chemistry and coating with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains. The treated support by 3-butenylamine hydrochloride improves the signal detection of recombinant protein, while surface modification with the 3,7-dimethylocta-2,6-dien-1-diamine (geranylamine) enhances the sensitivity of the native protein. Beside the surface chemistry effect, these different results are associated with protein conformation. [less ▲]

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See detailSurface Treatment of Polymeric Materials Controlling the Adhesion of Biomolecules
Poncin-Epaillard, Fabienne; Vrlinic, Tjasa; Mozetic, Miran et al

in Journal of Functional Biomaterials (2012), 3

This review describes different strategies of surface elaboration for a better control of biomolecule adsorption. After a brief description of the fundamental interactions between surfaces and ... [more ▼]

This review describes different strategies of surface elaboration for a better control of biomolecule adsorption. After a brief description of the fundamental interactions between surfaces and biomolecules, various routes of surface elaboration are presented dealing with the attachment of functional groups mostly thanks to plasma techniques, with the grafting to and from methods, and with the adsorption of surfactants. The grafting of stimuli-responsive polymers is also pointed out. Then, the discussion is focused on the protein adsorption phenomena showing how their interactions with solid surfaces are complex. The adsorption mechanism is proved to be dependent on the solid surface physicochemical properties as well as on the surface and conformation properties of the proteins. Different behaviors are also reported for complex multiple protein solutions. [less ▲]

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See detailDecontamination of Prions by the Flowing Afterglow of a Reduced-Pressure N2-O2 Cold-Plasma
Elmoualij, Benaïssa ULg; Thellin, Olivier ULg; GOFFLOT, Stéphanie ULg et al

in Plasma Processes and Polymers (2012), 9(6), 612618

Transmissible spongiform encephalopathies, also called prion diseases, represent a family of neurodegenerative disorders that affect various animal species. Since the infectious forms of prions are ... [more ▼]

Transmissible spongiform encephalopathies, also called prion diseases, represent a family of neurodegenerative disorders that affect various animal species. Since the infectious forms of prions are transmissible and highly resistant to chemical and physical decontamination methods routinely used in healthcare, they represent a challenge for science, medicine, and public health/food systems. Suitable decontamination procedures have been proposed, but they are generally incompatible with the materials from which medical devices are made. In this study, we evaluate a cold gaseous-plasma treatment, based on the outflow from a N2[BOND]O2 microwave discharge, as an alternative decontamination tool against both the non-infectious (PrPC) and infectious (PrPSc) forms of prion proteins. The efficiency of the plasma treatment on these proteins is assessed using an in vitro assay as well as an in vivo bioassay. We showed by Enzyme-Linked Immuno-Sorbent Assay (ELISA) that the N2[BOND]O2 discharge afterglow reduces the immunoreactivity of both non-infectious recombinant and pathogenic prion proteins deposited on polystyrene substrates. Tests done in vivo demonstrate that exposure to the cold-plasma flowing afterglow achieves significant decontamination of stainless steel sutures inoculated with infectious forms of prions to be implanted in mice. [less ▲]

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See detailIncreasing the Detection Limit of the Parkinson Disorder through a Specific Surface Chemistry Applied onto Inner Surface of the Titration Well
Mille, Caroline; Debarnot; Zorzi, Willy ULg et al

in Journal of Functional Biomaterials (2012), 2

The main objective of this paper was to illustrate the enhancement of the sensitivity of ELISA titration for neurodegenerative proteins by reducing nonspecific adsorptions that could lead to false ... [more ▼]

The main objective of this paper was to illustrate the enhancement of the sensitivity of ELISA titration for neurodegenerative proteins by reducing nonspecific adsorptions that could lead to false positives. This goal was obtained thanks to the association of plasma and wet chemistries applied to the inner surface of the titration well. The polypropylene surface was plasma-activated and then, dip-coated with different amphiphilic molecules. These molecules have more or less long hydrocarbon chains and may be charged. The modified surfaces were characterized in terms of hydrophilic—phobic character, surface chemical groups and topography. Finally, the coated wells were tested during the ELISA titration of the specific antibody capture of the α-synuclein protein. The highest sensitivity is obtained with polar (Θ = 35°), negatively charged and smooth inner surface. [less ▲]

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See detailCerebrospinal Fluid Collection Tubes: a critical issue for Alzheimer Disease diagnosis
Perret-Liaudet, Armand; Pelpel, Mathieu; Tholance, Yannick et al

in Clinical Chemistry (2012), 58

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See detailRisk of Alzheimer’s Disease biological misdiagnosis linked to cerebrospinal collection tubes
Perret-Liaudet A; Pelpel M,; Tholance Y et al

in Journal of Alzheimer's Disease [=JAD] (2012), 31((1)), 13-20

Tau proteins and amyloid-β (Aβ) peptides are the current recognized cerebrospinal fluid (CSF) biomarkers used as an aid in the diagnosis of Alzheimer's disease (AD). However, there is no consensus on ... [more ▼]

Tau proteins and amyloid-β (Aβ) peptides are the current recognized cerebrospinal fluid (CSF) biomarkers used as an aid in the diagnosis of Alzheimer's disease (AD). However, there is no consensus on their clinical use due to non-qualified cut-off values, probably related to the observed high pre-analytical and analytical variability. Standardized pre-analytical protocols have therefore been proposed. Importantly, these recommend the use of polypropylene collection/sampling tubes while, to date, no broad comparison of these types of tubes has been conducted. In this study, we first compared, as part of a real clinical workflow, the impact of four different collection tubes on the CSF concentration of Aβ peptides (Aβ42, Aβ40) and total (hTau) and phosphorylated (P-Tau181P) tau proteins measured using routine ELISA kits. We then extended this study to 11 polypropylene tubes used by different clinical laboratories, and investigated their plastic polymer composition using differential scanning calorimetry and Fourier Transformed Infrared spectroscopy. Significant concentration variations linked solely to the use of different types of tubes were observed. This was particularly marked for Aβ peptides, with >50% disparity occurring in less than five minutes. Polymer composition analysis revealed that most polypropylene tubes were in fact copolymers with at least polyethylene. There was no clear correlation between tube composition and pre-analytical behavior. Our results show that the use of polypropylene tubes does not guarantee satisfactory pre-analytical behavior. They also point to collection/sampling tubes being a major pre-analytical source of variability that could impact the significance of AD biological diagnosis. [less ▲]

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See detailSterilization/disinfection of medical devices using plasma: the reduced-pressure flowing-afterglow of the N2-O2 discharge as the inactivating medium
Michel Moisan; Jean Barbeau; Karim Boudama et al

in European journal of Applied Physics (2012)

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See detailPre-Mortem Diagnostic Screening for Transmissible Spongiform Encephalopathies by Proteomic Approaches
Falisse-Poirrier, Nandini; Thellin, Olivier ULg; Dupiereux-Fettweis, Ingrid ULg et al

Book published by Nova Publishers (2012)

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See detailHow to control the recombinant prion protein adhesion for successful storage through modification of surface properties Biointerphases
Vrlinic; Debarnot, Dominique; Legeay, Gilbert et al

in Biointerphases (2012)

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See detailNon-Adhesive Behavior of New Nanostructured PNIPAM Surfaces Towards Specific Neurodegenerative Proteins: Application to Storage and Titration of Tau Proteins
Vrlinic, Tjasa; Debarnot, Dominique; Legeay, Gilbert et al

in Macromolecular Bioscience (2012)

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See detailAre the interactions between recombinant prion protein and polymeric surfaces related to the hydrophilic and hydrophobic balance ?
Vrlinic, Tjasa; Debarnot, Dominique; Legeay, Gilbert et al

in Macromolecular Bioscience (2012), In press

In press

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See detailAre the interactions between neurodegenerative proteins and polymeric surfaces related to the hydrophilic and hydrophobic balance ?
Vrlinic; Debarnot, Dominique; Legeay, Gilbert et al

in Macromolecular Bioscience (2012), In press

In press

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See detailImprovement of the Detection of Neurodegenerative Alzheimer's Disease through a Specific Surface Chemistry Applied onto the Inner Surface of the Titration Well.
Mille, Caroline; Debarnot, Dominique; Zorzi, Willy ULg et al

in Journal of Biomaterials Science. Polymer Edition (2011)

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See detailStudy of the Adhesion of Neurodegenerative Proteins on Plasma-Modified and Coated Polypropylene Surfaces.
Poncin-Epaillard, Fabienne; Mille, Caroline; Debarnot et al

in Journal of Biomaterials Science. Polymer Edition (2011)

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See detailDetection of Clostridium botulinum neurotoxins A and B in milk by ELISA and immuno-PCR at higher sensitivity than mouse bio-assay
Rajkovic, Andreja; Elmoualij, Benaïssa ULg; Fikri, Youssef et al

in Food Analytical Methods (2011), [Epub ahead of print]

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See detailAlzheimer’s diseases: towards biomarkers for an early diagnosis
Elmoualij, Benaïssa ULg; Dupiereux-Fettweis, Ingrid ULg; Seguin, jérémie et al

Book published by InTech (2011)

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See detailKu proteins interact with activator protein-2 transcription factors and contribute to ERBB2 overexpression in breast cancer cell lines.
Nolens, Grégory ULg; Pignon, Jean-Christophe ULg; Koopmansch, Benjamin ULg et al

in Breast Cancer Research [=BCR] (2009), 11(6),

INTRODUCTION: Activator protein-2 (AP-2) alpha and AP-2 gamma transcription factors contribute to ERBB2 gene overexpression in breast cancer. In order to understand the mechanism by which the ERBB2 gene ... [more ▼]

INTRODUCTION: Activator protein-2 (AP-2) alpha and AP-2 gamma transcription factors contribute to ERBB2 gene overexpression in breast cancer. In order to understand the mechanism by which the ERBB2 gene is overexpressed we searched for novel AP-2 interacting factors that contribute to its activity. METHODS: Ku proteins were identified as AP-2 alpha interacting proteins by glutathione serine transferase (GST)-pull down followed by mass spectrometry. Transfection of the cells with siRNA, expression vectors and reporter vectors as well as chromatin immunoprecipitation (ChIP) assay were used to ascertain the implication of Ku proteins on ERBB2 expression. RESULTS: Nuclear proteins from BT-474 cells overexpressing AP-2 alpha and AP-2 gamma were incubated with GST-AP2 or GST coated beads. Among the proteins retained specifically on GST-AP2 coated beads Ku70 and Ku80 proteins were identified by mass spectrometry. The contribution of Ku proteins to ERBB2 gene expression in BT-474 and SKBR3 cell lines was investigated by downregulating Ku proteins through the use of specific siRNAs. Depletion of Ku proteins led to downregulation of ERBB2 mRNA and protein levels. Furthermore, reduction of Ku80 in HCT116 cell line decreased the AP-2 alpha activity on a reporter vector containing an AP-2 binding site linked to the ERBB2 core promoter, and transfection of Ku80 increased the activity of AP-2 alpha on this promoter. Ku siRNAs also inhibited the activity of this reporter vector in BT-474 and SKBR3 cell lines and the activity of the ERBB2 promoter was further reduced by combining Ku siRNAs with AP-2 alpha and AP-2 gamma siRNAs. ChIP experiments with chromatin extracted from wild type or AP-2 alpha and AP-2 gamma or Ku70 siRNA transfected BT-474 cells demonstrated Ku70 recruitment to the ERBB2 proximal promoter in association with AP-2 alpha and AP-2 gamma. Moreover, Ku70 siRNA like AP-2 siRNAs, greatly reduced PolII recruitment to the ERBB2 proximal promoter. CONCLUSIONS: Ku proteins in interaction with AP-2 (alpha and gamma) contribute to increased ERBB2 mRNA and protein levels in breast cancer cells. [less ▲]

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See detailPrion: Biosafety Level 3-unconventional transmissible agent
Zorzi, Willy ULg; Elmoualij, Benaïssa ULg

Conference (2009, April 22)

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