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See detailVERFAHREN ZUR VERÄNDERUNG DES ATP-ADP-VERHÄLTNISSES IN ZELLEN
Vigeolas, Hélène ULg; Zank, thorsten; Oswald, Oliver et al

Patent (2007)

(FR) L'invention concerne un procédé de modification du rapport ATP/ADP dans une cellule, un tissu, un organe, un micro-organisme ou une plante par modification de l'activité d'une protéine à hème dans la ... [more ▼]

(FR) L'invention concerne un procédé de modification du rapport ATP/ADP dans une cellule, un tissu, un organe, un micro-organisme ou une plante par modification de l'activité d'une protéine à hème dans la cellule. L'invention porte également sur l'application de ce procédé. [less ▲]

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See detailVERFAHREN ZUR ERHÖHUNG DES GESAMTÖLGELHALTES IN ÖLPFLANZEN
Vigeolas, Hélène ULg; Zank, Thorsten; Oswald, Oliver et al

Patent (2007)

DE) Die Erfindung betrifft Verfahren zur Erhöhung des Gesamtölgehaltes und/oder des Gehalts an Glycerol-3-Phosphat in transgenen Ölpflanzen, die mindestens 20 Gew-% Ölsäure bezogen auf den ... [more ▼]

DE) Die Erfindung betrifft Verfahren zur Erhöhung des Gesamtölgehaltes und/oder des Gehalts an Glycerol-3-Phosphat in transgenen Ölpflanzen, die mindestens 20 Gew-% Ölsäure bezogen auf den Gesamtfettsäuregehalt enthalten, bevorzugt in pflanzlichen Samen, durch Expression von Glycerol-3-phosphatdehydrogenasen (G3PDH) aus Hefen, bevorzugt aus Saccharomyces cerevisiae. Vorteilhaft wird das im Verfahren gewonnene Öl und/oder die freien Fettsäure Polymeren, Nahrungsmitteln, Futtermitteln, Kosmetika, Pharmazeutika oder Produkten mit industriellen Anwendungen zugesetzt. [less ▲]

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See detailIncreasing seed oil content in oil-seed rape (Brassica napus L.) by over-expression of a yeast glycerol-3-phosphate dehydrogenase under the control of a seed-specific promoter.
Vigeolas, Hélène ULg; Waldeck, Peter; Zank, Thorsten et al

in Plant Biotechnology Journal (2007), 5(3), 431-41

Previous attempts to manipulate oil synthesis in plants have mainly concentrated on the genes involved in the biosynthesis and use of fatty acids, neglecting the possible role of glycerol-3-phosphate ... [more ▼]

Previous attempts to manipulate oil synthesis in plants have mainly concentrated on the genes involved in the biosynthesis and use of fatty acids, neglecting the possible role of glycerol-3-phosphate supply on the rate of triacylglycerol synthesis. In this study, a yeast gene coding for cytosolic glycerol-3-phosphate dehydrogenase (gpd1) was expressed in transgenic oil-seed rape under the control of the seed-specific napin promoter. It was found that a twofold increase in glycerol-3-phosphate dehydrogenase activity led to a three- to fourfold increase in the level of glycerol-3-phosphate in developing seeds, resulting in a 40% increase in the final lipid content of the seed, with the protein content remaining substantially unchanged. This was accompanied by a decrease in the glycolytic intermediate dihydroxyacetone phosphate, the direct precursor of glycerol-3-phosphate dehydrogenase. The levels of sucrose and various metabolites in the pathway from sucrose to fatty acids remained unaltered. The results show that glycerol-3-phosphate supply co-limits oil accumulation in developing seeds. This has important implications for strategies that aim to increase the overall level of oil in commercial oil-seed crops for use as a renewable alternative to petrol. [less ▲]

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