References of "Wouters-Ballman, Patricia"
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See detailPurification and characterization of a bovine pregnancy-associated glycoprotein
Zoli, André Pagnah; Beckers, Jean-François ULg; Wouters-Ballman, Patricia et al

in Biology of Reproduction (1991), 45(1), 1-10

A 67000 Mr bovine pregnancy-associated glycoprotein (bPAG) has been isolated from fetal cotyledons and purified to homogeneity by HPLC. The purification was monitored by a double immunodiffusion test and ... [more ▼]

A 67000 Mr bovine pregnancy-associated glycoprotein (bPAG) has been isolated from fetal cotyledons and purified to homogeneity by HPLC. The purification was monitored by a double immunodiffusion test and by RIA in conjunction with an antiserum raised against a crude fraction of placenta-specific antigens. The molecular weight of bPAG was estimated to be 67000 by SDS-PAGE. The isoelectric points (pI) of the four isoforms, determined by high-resolution analytical electrofocusing in polyacrylamide gel, were 4.4, 4.6, 5.2, and 5.4. The carbohydrate content of the bPAG consisted of approximately 10.02 +/- 1.09% neutral sugar and variant amounts of sialic acid (from 0.29 +/- 0.06% in the most basic isoform to 2.1 +/- 0.31% in the most acidic isoform). A specific antiserum was raised against the purified bPAG. A specific RIA showed that the bPAG was antigenically unrelated to BSA, alphafetoprotein (AFP), and human schwangerschafts-spezifischen (pregnancy-specific) beta 1 glycoprotein (SP1). According to some characteristics (e.g. the molecular weight), the purified bPAG may correspond to a form of the pregnancy-specific protein B previously described by Sasser and colleagues (Biol Reprod 1986; 35:936-942). [less ▲]

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See detailDosage radioimmunologique de l'hormone placentaire somatotrope et mammotrope bovine
Beckers, Jean-François ULg; De Coster, R.; Wouters-Ballman, Patricia et al

in Annales de Médecine Vétérinaire (1982), 126

Highly specific antisera were obtained from rabbits immunized with a purified preparation of Bovine Placental Somatomammotropin leading to the development of a sensitive radioimmunoassay. Teh values in ... [more ▼]

Highly specific antisera were obtained from rabbits immunized with a purified preparation of Bovine Placental Somatomammotropin leading to the development of a sensitive radioimmunoassay. Teh values in cotyledons extracts and foetal serum given by this methos are in agreement with those determined by radioreceptor assay using rabbit membranous receptors. Placental somatomammotrophic hormone is always present in pregnant cow sera from day 110 after fertilization but at low concentration (<=2 ng/ml) [less ▲]

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See detailIsolement d'une hormone placentaire bovine présentant une activité analogue à la prolactine et à l'hormone de croissance
Beckers, Jean-François ULg; Fromont-Liénard, Ch; Van Der Zwalmen, P. et al

in Annales de Médecine Vétérinaire (1980), 124

Isolation from bovine cotyledon extracts of an hormone with somatotrophic and mammotrophic activity was performed using this hormone during the successive steps of purification, its prolactinic and ... [more ▼]

Isolation from bovine cotyledon extracts of an hormone with somatotrophic and mammotrophic activity was performed using this hormone during the successive steps of purification, its prolactinic and somatotrophic activity was determined by radioreceptor assay. The purified hormone shows a prolactinic activity similar to that of PRL-NIH-B4 standard and a somatotrophic activity three times that of GH-NIH-B18 standard. [less ▲]

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See detailVariations nycthémérales de la testostérone et de la lutropine plasmatiques chez le chien
De Coster, R.; Beckers, Jean-François ULg; Wouters-Ballman, Patricia et al

in Annales de Médecine Vétérinaire (1979), 123

Diurnal variations of plasmatic testosterone and lutropin were determined by radioimmunoassay in seven male beagle dogs. Blood samples were withdrawn hourly during 24 hours or every 20 min during 8 hours ... [more ▼]

Diurnal variations of plasmatic testosterone and lutropin were determined by radioimmunoassay in seven male beagle dogs. Blood samples were withdrawn hourly during 24 hours or every 20 min during 8 hours. LH concentrations ranged from 1.2+/-0.6 ng/ml (basal level) to 3.8+/-1.6ng/ml (peak level). Testosterone concentration varied from 1.4+/-0.8ng/ml to 3.5+/-1.8ng/ml. 5 to 8 LH peaks were observed during the 24 hours period and they were generally followed, after about 60 min., by corresponding testosterone peaks. On the other hand, no regular nycthemeral rythm could be established. [less ▲]

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See detailVariations de la LH, de la FSH, du 17 ß oestradiol et de la progestérone au cours du cycle oestral de la chienne
De Coster, R.; Beckers, Jean-François ULg; Wouters-Ballman, Patricia et al

in Annales de Médecine Vétérinaire (1979), 123

Plasma levels of progesterone, 17-beta-oestradiol, LH and FSH were measured by radioimmunoassay in five beagle bitches during two consecutive estrous cycles. Progesterone level rises at the end of ... [more ▼]

Plasma levels of progesterone, 17-beta-oestradiol, LH and FSH were measured by radioimmunoassay in five beagle bitches during two consecutive estrous cycles. Progesterone level rises at the end of proestrus to reach 40 to 50ng/ml and then decreases progressively to its basal level (0.64 ng/ml), attained 80 to 100 days after oestus. A 17-beta-oestradiol peak (75.1 pg/ml) is evident in proestrus and reaches its maximum level 24h before LH surge. The fall of estrogen leads to a discharge of gonadotropins lasting 48 to 72 hours. The FSH surge reaches his maximum level 48 h after that of LH. Pregnancy does not change the secretory pattern of these hormones but progesterone falls just before parturition. [less ▲]

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See detailInduction de l'oestrus chez les génisses en anoestrus fonctionnel
Beckers, Jean-François ULg; Wouters-Ballman, Patricia; Ectors, Francis ULg et al

in Annales de Médecine Vétérinaire (1978), 122

Fuctional anoestrus is frequently encountered during winter months and most specially in beef cattle. Ovaries and genital tract display a resting, dormant conditions. Progesterone, 17-beta-oestradiol and ... [more ▼]

Fuctional anoestrus is frequently encountered during winter months and most specially in beef cattle. Ovaries and genital tract display a resting, dormant conditions. Progesterone, 17-beta-oestradiol and LH as measured by radioimmunoassay remain at a very low level. Insertion of an intravaginal device "Abbovestrol Prid" (progesterone 2.3 g 17-beta-oestradiol 10mg) is successful. During the course of the treatment; progesterone reaches normal (corpus luteum induced) levels; 17-beta-oestradiol which is at first very high decreases to a mean value of 4-5 pg/ml; LH remains at a basal level but rises slowly at the end of the treatment. Removal of the insert is generally (16/20) followed by oestrus after c.a. 48 hours. This oestrus is characterized by a normal hormonal profile. However, a PMSG injection when the insert is removed raises chances of favourable result. [less ▲]

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See detailEvolution du taux de cortisol plasmatique chez le veau de la naissance à l'âge de 14 jours
Massip, Alban; Wouters-Ballman, Patricia; Beckers, Jean-François ULg et al

in Comptes Rendus de l'Académie des Sciences de Paris (1977), 285

Plasma cortisol was recorded in 15 calves from birth to 14 days of age by a radioimmunoassay technique. At birth mean cortisol value is 67.3 ng/ml; 6 hours later it decreases by 58.4% then it gradually ... [more ▼]

Plasma cortisol was recorded in 15 calves from birth to 14 days of age by a radioimmunoassay technique. At birth mean cortisol value is 67.3 ng/ml; 6 hours later it decreases by 58.4% then it gradually diminishes and about 14 days after, it reaches adult levels (5.1 ng/ml). [less ▲]

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