References of "Winkler-Gol, Rose"
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See detailA New Cis Element Is Involved in the Her2 Gene Overexpression in Human Breast Cancer Cells
Grooteclaes, Madeleine; Vernimmen, Douglas; Plaza, S. et al

in Cancer Research (1999), 59(11), 2527-31

The HER2 proto-oncogene product is overexpressed in 30% of breast cancers, and this correlates with poor prognosis. Increased levels of HER2 mRNA in breast cancer cell lines result from increased gene ... [more ▼]

The HER2 proto-oncogene product is overexpressed in 30% of breast cancers, and this correlates with poor prognosis. Increased levels of HER2 mRNA in breast cancer cell lines result from increased gene transcription. We report the identification of a new 17-bp-long cis sequence located between positions -506 and -489 from the transcription start site. This sequence is recognized by a trans-activating factor that we tentatively named HER2 transcription factor (HTF). This factor, involved in the increased transcription of the HER2 gene in the BT-474 mammary tumor cells, has a molecular weight of about Mr 50,000. HTF can also bind, but with a lower affinity, to a related cis sequence present in the epidermal growth factor receptor promoter. Interestingly, the HTF binding activity is high in nuclear extracts from several mammary tumor cells overexpressing the HER2 gene. [less ▲]

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See detailIdentification du site de fixation d'un facteur de transcription contribuant à la surexpression du gène c-erbB2 dans les cellules tumorales BT-474.
Grooteclaes, Madeleine; Pasleau, Françoise ULg; Hodzic, Didier et al

Conference (1996, February 14)

Detailed reference viewed: 17 (3 ULg)
See detailIdentification of the cis element recognized by a transcription factor specific to the tumor BT-474 mammary cells.
Grooteclaes, Madeleine; Pasleau, Françoise ULg; Hodzic, Didier et al

Conference (1996, February 03)

Detailed reference viewed: 7 (0 ULg)
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See detailSpecific transcription factors control the c-erbB2 gene expression in the mammary tumor BT-474 cells.
Grooteclaes, Madeleine; Pasleau, Françoise ULg; Garsoux, G. et al

Conference (1995, December)

Detailed reference viewed: 18 (1 ULg)
See detailDes facteurs de transcription spécifiques contrôlent l'expression du gène c-erbB2 dans les cellules épithéliales mammaires tumorales BT-474.
Grooteclaes, Madeleine; Pasleau, Françoise ULg; Winkler-Gol, Rose

Conference (1995, February 16)

Detailed reference viewed: 10 (0 ULg)
See detailSpecific transcription factors of the tumor BT-474 mammary cells.
Grooteclaes, Madeleine; Pasleau, Françoise ULg; Winkler-Gol, Rose

Conference (1995, January 21)

Detailed reference viewed: 8 (0 ULg)
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See detailThe 6-Kilobase C-Erbb2 Promoter Contains Positive and Negative Regulatory Elements Functional in Human Mammary Cell Lines
Grooteclaes, Madeleine; Pasleau, Françoise ULg; Dijkmans, Huguette et al

in Cancer Research (1994), 54(15), 4193-9

A 6-kilobase fragment extending at the 5'-end of the c-erbB2 protooncogene was isolated from a normal human lymphocyte genomic DNA library. The full-length fragment and five subfragments with identical 3 ... [more ▼]

A 6-kilobase fragment extending at the 5'-end of the c-erbB2 protooncogene was isolated from a normal human lymphocyte genomic DNA library. The full-length fragment and five subfragments with identical 3'-ends were obtained by progressive unidirectional deletion from the 5'-end and were cloned in front of the luciferase reporter gene. The hybrid genes were analyzed for transcriptional activity in human mammary cell lines synthesizing low (HBL-100 and T-47D), moderate (MDA-MB-453), or high (BT-474) amounts of the c-erbB2 mRNA and were also analyzed in HeLa cells. Gene-specific expression was observed, indicating the presence of multiple cis-acting sequences in the c-erbB2 promoter. A major negative element is located in the -2- to -4-kilobase region. It is flanked on both sides by positive elements that display enhanced transcriptional activity in the BT-474 tumor cells only. While predominant in the low-expressing cells, the effect of the repressor appears to be overcome by the distal transactivator in the high-expressing BT-474 cells, resulting in a 15 to 50 times increase in luciferase activity relative to the HBL-100 and T-47D cells, respectively. Cell-specific expression relies on the trans-acting factors present in the different cell lines. The formation of cell-specific protein-DNA complexes was demonstrated by gel retardation assay. [less ▲]

Detailed reference viewed: 27 (4 ULg)