  Assessment of the cell-mediated immunity in cattle infection after bovine herpesvirus 4 infection, using an in vitro antigen-specific interferon-gamma assay.; ; et al in Veterinary Microbiology (1996), 53(1-2), 133-41 The cell-mediated immunity (CMI) following bovine herpesvirus 4 (BHV4) infection has been poorly investigated in cattle. The in vivo response measured by a delayed type of hypersensitivity (DTH) assay has ... [more ▼] The cell-mediated immunity (CMI) following bovine herpesvirus 4 (BHV4) infection has been poorly investigated in cattle. The in vivo response measured by a delayed type of hypersensitivity (DTH) assay has been reported to be positive in only few animals showing serological evidences of BHV4 infection. We have investigated the CMI following BHV4 infection by an in vitro antigen-specific interferon gamma (IFN-gamma) release assay, as an indicator of an actively acquired immunity to BHV4. Our preliminary results using a partially purified antigen suggest that there was a measurable CMI in 75 out of 168 animals (44.4%) originating from a farm with a clinical history and serological evidences (76.3% seropositivity) of BHV4 infection. If the results of serological tests and BHV4 IFN-gamma test are interpreted in parallel, 81.5% of the animals are classified positive, demonstrating the complementarity of these tests. The specificity of the BHV4 IFN-gamma test was supported by the absence of a measurable CMI in 41 animals originating from a farm with no clinical history or serological evidence of BHV4 infection. In an allied study, we developed a bovine herpesvirus 1 (BHV1) IFN-gamma test. This allowed us to measure the antigen specific IFN-gamma release after stimulation with a mixture of BHV1 and BHV4 antigens. Animals that were classified negative by the BHV4 IFN-gamma test and by the BHV1 IFN-gamma test, were classified negative after stimulation with a mixture of both antigens. Animals that were classified positive by the BHV4 IFN-gamma test or the BHV1 IFN-gamma test, were classified positive after stimulation with a mixture of both antigens. Taken together these results suggest that the in vitro assessment of the CMI after BHV4 infection should be further investigated as a specific and valuable alternative to the DTH assay. [less ▲] Detailed reference viewed: 33 (0 ULg)Effect of Repeated Intradermal Injections of Bovine Herpesvirus Type 1 Antigen on Seronegative CattleThiry, Etienne  ; ; et alin Veterinary Record : Journal of the British Veterinary Association (1992), 130(17), 372-5 Forty-three cattle seronegative to bovine herpesvirus-1 (BHV-1) were given from one to five intradermal injections of BHV-1 inactivated antigen at four-week intervals. This delayed hypersensitivity test ... [more ▼] Forty-three cattle seronegative to bovine herpesvirus-1 (BHV-1) were given from one to five intradermal injections of BHV-1 inactivated antigen at four-week intervals. This delayed hypersensitivity test was assessed by the increase in skin thickness. The activity of the antigen was assessed in five animals which had a previous natural BHV-1 infection with clinical signs and seroconversion. Anti-BHV-1 antibodies were detected by seroneutralisation and an enzyme-linked immunoassay. Only one animal showed a significant but slight increase in skin thickness after the first test, but it was negative after a second test. The animals remained seronegative after the first test. Seroconversion was identified in 11 of the 43 animals (25 per cent) submitted to repeated delayed hypersensitivity tests. Five of 37 animals seroconverted after only two tests. The serological response was transient in seven of 11 seroconverted calves. Repeated hypersensitivity tests were therefore able to induce a serological response in seronegative calves but the response was weak and often transient. The test must therefore be applied cautiously to seronegative animals. [less ▲] Detailed reference viewed: 3 (0 ULg)Bovine Herpesvirus 4 Isolates: A Comparison of Three Major Glycoproteins; ; et al in Veterinary Microbiology (1991), 29(3-4), 251-9 Twenty-four Belgian field isolates of bovine herpesvirus 4 (BHV-4), together with four reference strains were compared by radio-immunoprecipitation and western blotting using a polyvalent antiserum and ... [more ▼] Twenty-four Belgian field isolates of bovine herpesvirus 4 (BHV-4), together with four reference strains were compared by radio-immunoprecipitation and western blotting using a polyvalent antiserum and monoclonal antibodies raised against major glycoproteins. Most of these strains showed the same protein profile as the European reference strain Movar 33/63. For two strains the molecular weight of gp 6, p (gp 10/gp 17) and gp 10 were the same as those of the American reference strain DN 599. No relationship could be established between the protein profiles and origin of the isolates or with the restriction patterns. This study provides a view of the molecular weight variations of the major BHV-4 glycoproteins among field isolates. [less ▲] Detailed reference viewed: 9 (2 ULg)Genomic Diversity among Bovine Herpesvirus 4 Field Isolates; ; et al in Archives of Virology (1991), 116(1-4), 1-18 Twenty-eight Belgian field isolates of bovine herpesvirus 4 (BHV-4) coming from a variety of clinical diseases have been studied by restriction analysis and Southern blot hybridization. The unique central ... [more ▼] Twenty-eight Belgian field isolates of bovine herpesvirus 4 (BHV-4) coming from a variety of clinical diseases have been studied by restriction analysis and Southern blot hybridization. The unique central part of the genome was very well conserved among strains; only one variation in a restriction site was detected in 3 isolates which contain an additional EcoRI site also present in the LVR 140 strain; three regions in the unique part of the genome varied in size, one of these was highly variable. The polyrepetitive fragments (prDNAs) situated in tandem at both genomic ends were also variable in size; most of the isolates exhibited prDNA units of one size (major prDNA) and some of them also contained prDNA units having a different size and present in a lower amount (minor prDNA) than the major prDNA. Other isolates possessed two major prDNAs of different sizes which were both present in the same genome. The left junction fragment between the unique and the repeated sequences was also highly variable. No relationship could be established between the restriction pattern and the origin of the isolates; patterns of isolates coming from the same herd were similar except in one case. This study provides a view of the genome variability existing between BHV-4 field isolates. [less ▲] Detailed reference viewed: 8 (0 ULg)Recherche des anticorps dirigés contre les BHV-1, BHV-2, BHV-4, le virus BVD-MD, les adenovirus A et B, le rotavirus et le coronavirus bovins chez des bovins de l'Ouest du Zaire: résultats complémentaires.; ; Thiry, Etienne et alin Revue d'Elévage et de Médecine Vétérinaire des Pays Tropicaux (1989), 42(2), 155-61 Two-hundred bovine sera from western Zaire were screened for antibodies to 8 viruses: BHV-1, BHV-2, BHV-4, BVD-MD virus, bovine adenovirus A and B, bovine rotavirus and bovine coronavirus. Positive sera ... [more ▼] Two-hundred bovine sera from western Zaire were screened for antibodies to 8 viruses: BHV-1, BHV-2, BHV-4, BVD-MD virus, bovine adenovirus A and B, bovine rotavirus and bovine coronavirus. Positive sera were found to all these viruses. For animals whose origin was undoubted, the main features were the high prevalence of infections by rotavirus and BHV-4 and the low prevalence of infections by coronavirus and BVD-MD virus. [less ▲] Detailed reference viewed: 22 (1 ULg)Study Of The Relationships Between Infection By Blv (Bovine Leukemia-Virus), Persistant Lymphocytosis And Infections By Other Infectious Agents In A Cattle Herd; ; et al in Annales de Médecine Vétérinaire (1989), 133(6), Detailed reference viewed: 10 (6 ULg) Aspects cliniques et anatomo-pathologiques de cas répétés d'orchite chez des taureaux reproducteurs en BelgiqueHanzen, Christian ; Dessy, Cécile ; Thiry, Etienne et alin Annales de Médecine Vétérinaire (1981), 125 Detailed reference viewed: 17 (7 ULg) |
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