  Identification and localization of the structural proteins of anguillid herpesvirus 1Van Beurden, Steven  ; ; et alin Veterinary Research (2011), 42(1), 105 Detailed reference viewed: 3 (1 ULg)Skin mucus of Cyprinus carpio inhibits cyprinid herpesvirus 3 binding to epidermal cells; Fournier, Guillaume ; Rakus, Krzysztof et alin Veterinary Research (2011), 42(92), Detailed reference viewed: 39 (19 ULg) The genome of cyprinid herpesvirus 3 encodes 40 proteins incorporated in mature virionsMichel, Benjamin ; ; Victor, Stalinraj et alin Journal of General Virology (The) (2010) Detailed reference viewed: 41 (21 ULg)Phosphorylation Of Histone Deacetylase 7 By Protein Kinase D Mediates T Cell Receptor-Induced Nur77 Expression And ApoptosisDequiedt, Franck ; ; et alin Journal of Experimental Medicine (2005), 201(5), Detailed reference viewed: 11 (3 ULg)Isolement et caractérisation de l'ostéocalcine équine; ; Amory, Hélène et alin Annales de Médecine Vétérinaire (2002), 146 Equine bone gamma-carboxyglutamic acid containing protein - also called osteocalcin - has been isolated for the first time till homogeneity. After acid demineralisation and acid extraction, the ... [more ▼] Equine bone gamma-carboxyglutamic acid containing protein - also called osteocalcin - has been isolated for the first time till homogeneity. After acid demineralisation and acid extraction, the purification procedure was based on reverse phase chromatography, gel filtration and ion exchange chromatography. The amino acid sequence of the primary structure has been determined to be YLDHWLGA(HYP) APYPDPL(GLA)PRR(GLA)VC (GLA)LNPDCDELADHIGF-AYRRFYGPV. The protein has a calculated molecular weight of 5,732 kDa and a calculated isoelectric point of 4,45. It contains 49 amino acids, from which 3 are gamma-carboxyglutamic acid residues at position 17, 21 and 24 and has a fully hydroxylated proline in position nine and cysteine in position 23 and 29. Equine osteocalcin shows a strong homology with bovine (98%), ovine (96%) and human (94%) osteocalcin [less ▲] Detailed reference viewed: 29 (8 ULg)Isolation ad Characterization of osteocalcin in horses; ; Amory, Hélène et alin Proceedings of the ECVS meeting 2002 (Resident Forum) (2002) Detailed reference viewed: 2 (0 ULg)Isolation and Radioimmunoassay Formation of Equine Osteocalcin- Preliminary Results; ; et al in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(1), 8-9 Detailed reference viewed: 2 (0 ULg)The isolation and characterization of equine Osteocalcin; ; Amory, Hélène et alin Proceedings of the 1st meeting of the International Bone and Mineral Society (IBMS) (2001) Identification of pregnancy-associated glycoprotein 1 (PAG-1) in zebu (Bos indicus) placentaMelo de Sousa, Noelita ; ; et alin Theriogenology (2001), 55(1), 327 Detailed reference viewed: 28 (1 ULg)Isolation of the pregnancy-associated glycoprotein 1 (PAG-1) from zebu (Bos indicus) placenta.Melo de Sousa, Noelita ; ; et alin Proceedings of the 16th Scientific Meeting of the European Embryo Transfer Association. (2000) Detailed reference viewed: 8 (0 ULg)Evidence For Specific And Non-Covalent Binding Of Lipids To Natural And Recombinant Mycobacterium Bovis Bcg Hsp60 Proteins, And To The Escherichia Coli Homologue Groel; ; et al in Microbiology-Sgm (2000), 146 Heat-shock proteins (Hsps) from various origins are known to share a conserved structure and are assumed to be key partners in the biogenesis of proteins. Fractionation of the mycobacterial Hsp60, a 65 ... [more ▼] Heat-shock proteins (Hsps) from various origins are known to share a conserved structure and are assumed to be key partners in the biogenesis of proteins. Fractionation of the mycobacterial Hsp60, a 65 kDa protein also called Cpn60, from Mycobacterium bovis BCG zinc-deficient culture filtrate on phenyl-Sepharose followed by Western blotting revealed the existence of four Hsp60-1 and Hsp60-2 forms, based on their hydrophobicity behaviour. Hsp60-2 species were further purified by ion-exchange chromatography and partial amino acid sequences of cyanogen bromide (CNBr) peptides of purified Hsp60-2 species showed identity with the amino acid sequence deduced from the hsp60-2 gene, indicating that the various Hsp60-2 forms are encoded by the same gene. In addition, the mycobacterial Hsp60-2 was overexpressed in E. coli using the pRR3Hsp60-2 plasmid and analysed on phenyl-Sepharose. The elution pattern of the recombinant Hsp60-2, as well as that of Escherichia coli GroEL, was similar to that of the native Hsp60-2 from the culture filtrate of M. bovis BCG and entirely different from that of the mycobacterial antigen 85. Extraction of mycobacterial Hsp60-2 forms, recombinant BCG Hsp60-2 and E. coli GroEL with organic solvents releases various amounts of non-covalently bound lipids. The presence of lipids on Hsp60-2 was confirmed by labelling M. bovis BCG with radioactive palmitate. The radioactivity was specifically associated with Hsp60 in the aqueous phase and the 19 and 38 kDa lipoproteins in the Triton X-114 phase. Analysis of the lipids extracted from purified Hsp60-2, recombinant BCG Hsp60-2 and E. coli GroEL by TLC showed the same pattern for all the samples. Acid methanolysis of the lipids followed by GC analysis led to the identification of C(16:0), C(18:0) and C(18:1) as the major fatty acyl constituents, and of methylglycoside in these proteins. Altogether, these data demonstrate that lipids are non-covalently bound to Hsp60-2 and homologous proteins. [less ▲] Detailed reference viewed: 3 (0 ULg)Isolation and Partial Characterization of a Pregnancy-Associated Glycoprotein Family from the Goat Placenta; ; et al in Biology of Reproduction (1998), 58(1), 109-115 Antigen(s) immunologically related to pregnancy-associated glycoproteins (PAGs) have previously been detected in the serum of pregnant goats. In this work, we describe a partial characterization of a ... [more ▼] Antigen(s) immunologically related to pregnancy-associated glycoproteins (PAGs) have previously been detected in the serum of pregnant goats. In this work, we describe a partial characterization of a family of PAGs isolated from the placenta of the goat. The procedure, monitored by RIA, included extraction of proteins at neutral pH, acidic, and ammonium sulfate precipitations; and gel filtration and ion exchange chromatographies. Immunoreactivity, initially located in the acidic supernatant and in the 40-80% ammonium sulfate fractions, was equally apportioned between the 0.04 and 0.08 M NaCl DEAE fractions. After further purification of both DEAE fractions, the preparations were subjected to one- and two-dimensional electrophoresis, and individual polypeptides were analyzed by amino acid sequencing. Three PAGs, which differed in amino acid sequence and apparent molecular masses (62, 59, and 55 kDa), were detected, each containing several isoforms with different pls: caprine (c) PAG62 (pl: 5.1, 4.8), cPAG59 (pl: 6.2, 5.9, 5.6), and cPAG55 (pl: 5.3, 5.1, 4.9). These proteins had high sequence identities to each other and to PAGs purified from other species. Each had two putative N-glycosylation sites within the 27 amino terminal residues sequenced. This work demonstrates that PAGs are present in goat placenta and that multiple forms are expressed. [less ▲] Detailed reference viewed: 14 (1 ULg)Identification of antigens recognized in the developing mouse brain by the RC2 antibody, a marker of radial gliaLeprince, Pierre ; ; et alin International Journal of Developmental Neuroscience (1996), 14(Sup. 1), 84 Detailed reference viewed: 13 (6 ULg) |
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