Does domestication process affect stress response in juvenile Eurasian perch Perca fluviatilis ?
; ; et al
in Comparative Biochemistry and Physiology. A : Comparative Physiology (2011), 159Detailed reference viewed: 33 (12 ULg)
DEVELOPMENT OF A LIQUID CHROMATOGRAPHY/TANDEM MASS SPECTROMETRY METHOD TO DETECT AND COMPARE KINETICS OF ENROFLOXACIN AND CIPROFLOXACIN ACCUMULATION AND DECONTAMINATION IN TRA CATFISH AND SHRIMP.
Scippo, Marie-Louise ; ; Widart, Joëlle et al
Poster (2011)Detailed reference viewed: 12 (2 ULg)
DETERMINATION AND KINETICS OF MALACHITE GREEN AND LEUCOMALACHITE GREEN IN TRA CATFISH (PANGASIONODON HYPOPHTHALMUS).
Scippo, Marie-Louise ; ; Baiwir, Dominique et al
Conference (2011)Detailed reference viewed: 4 (1 ULg)
Determination and kinetics of enrofloxacin and ciprofloxacin in Tra catfish (Pangasianodon hypophthalmus) and giant freshwater prawn (Macrobrachium rosenbergii) using a liquid chromatography/mass spectrometry method
Danyi, Sophie ; Widart, Joëlle ; Douny, Caroline et al
in Journal of Veterinary Pharmacology & Therapeutics (2011), 34(2), 142-152Detailed reference viewed: 87 (28 ULg)
DETERMINATION AND KINETICS OF MALACHITE GREEN AND LEUCOMALACHITE GREEN IN TRA CATFISH (PANGASIONODON HYPOPHTHALMUS)
Danyi, Sophie ; Baiwir, Dominique ; Pham Kim, Dang et al
Poster (2010, June)Detailed reference viewed: 59 (14 ULg)
Expression characteristics of potential biomarker genes in Tra catfish, Pangasianodon hypophthalmus, exposed to trichlorfon
; ; et al
in Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics (2010), 5(3), 207-216
Trichlorfon (TRC) is the most common organophosphorous insecticide used in aquaculture practices in Southeast Asian countries. Indiscriminate use of TRC can either damage or alter the enzymatic and ... [more ▼]
Trichlorfon (TRC) is the most common organophosphorous insecticide used in aquaculture practices in Southeast Asian countries. Indiscriminate use of TRC can either damage or alter the enzymatic and hormonal activities in the living organisms. In this present study, therefore, toxicogenomic analyses using real time PCR was used to characterize expression levels of various genes in Pangasianodon hypophthalmus after exposure to three concentrations, the 96 h 1/100LC50 (0.01 mg/L), the 96 h 1⁄10LC50 (0.1 mg/L) and the 96 h 1⁄2LC50 (0.5 mg/L) of TRC for 6 h, 24 h, 96 h, 7 days, 14 days, 28 days and 56 days respectively. The expression kinetics of stress and other cellular toxicity representative genes such as heat shock protein70 (HSP70), growth hormone, acetylcholinesterase (AChE), trypsinogen, cytochrome P4501B (CYP1B) and cytochrome oxidase subunit 1 (COI) were investigated in liver and gills. TRC at a level of 0.1 mg/L and 0.5 mg/L induced a time and dose-dependent increase in the expression of the HSP70, COI and CYPIB while the transcript level of AChE, growth hormone and trypsinogen were significantly down-regulated. These results could permit to develop a ‘molecular biomarker system’ which can be applied as a first-tier method of identifying contaminant exposure before effects at population level occur. [less ▲]Detailed reference viewed: 51 (4 ULg)
Development of a liquid/tandem mass spectrometry method to study kinetics of enrofloxacin and ciprofloxacin elimination in two species of aquaculture products
Danyi, Sophie ; Widart, Joëlle ; Remacle, Anne-Sophie et al
Poster (2009, May 19)Detailed reference viewed: 78 (25 ULg)
Adverse effects of enrofloxacin when associated with environmental stress in Tra catfish (Pangasianodon hypophthalmus).
; ; et al
in Chemosphere (2009), 77(11), 1577-84
The aim of this study was to assess the adverse effects of enrofloxacin (EF) on Tra catfish, Pangasianodon hypophthalmus, in relation with density stress. Fish were held at 40, 80 or 120 fish m(-3) and ... [more ▼]
The aim of this study was to assess the adverse effects of enrofloxacin (EF) on Tra catfish, Pangasianodon hypophthalmus, in relation with density stress. Fish were held at 40, 80 or 120 fish m(-3) and fed with pellets containing either 1 g kg(-1) EF or no EF. Antibiotic exposure lasted 7d and all fish were fed without EF for another 7-d recovery period. Fish were sampled at 3, 7, 8, 10 and 14 d after the beginning of EF exposure. Lipid peroxidation (LPO) and total glutathione (GSH) levels, catalase (CAT), glutathione-s-transferase (GST) and acetylcholine-esterase (AChE) activities were assessed in gill, brain, liver and muscle. At day 7, LPO levels in gills of EF-fish reared at low or high density were significantly more than 5-fold higher than their respective control. On the contrary, LPO in gills of EF-fish reared at medium density was significantly 3-fold lower than the control fish. Similarly, CAT activities in gills of EF-fish reared under low or high density were higher than in their control groups, while this activity was lower in EF-fish of the medium density group. AChE activities in muscles of EF-fish reared at low or high density were lower than controls at days 3 and 7, respectively. These results suggest that EF exposure may lead to disorders like lipid peroxidation and neural dysfunction in fish. However, when reared under lower stress condition (medium density), they may cope better with EF-induced stress than chronically stressed fish (low or high density). [less ▲]Detailed reference viewed: 74 (26 ULg)
Effect of partial or total replacement of forage fish by a dry diet on the quality of reproduction in pikeperch, Sander lucioperca
; ; et al
in Aquaculture Research (2009), 40(3), 376-383
The aim of this preliminary study was to evaluate the influence of different feeding regimes on the quality of reproduction in pikeperch. Three diets were tested: forage fish (FF), a commercial dry feed ... [more ▼]
The aim of this preliminary study was to evaluate the influence of different feeding regimes on the quality of reproduction in pikeperch. Three diets were tested: forage fish (FF), a commercial dry feed (DD) and a mix of both (FD). The diets were given to fish throughout a complete reproductive cycle. During the spawning season, couples were injected with human chorionic gonadotropin (hCG) and let to spawn on nests. Proportion of running males, spawning and hatching success and larval quality (weight, length, body protein, total lipid, fatty acid and lipid class compositions and resistances to osmotic shock and starvation stress tests at hatching) were evaluated. The proportion of running male was lower in the DD group than in the FF and FD groups (54% for DD against 76-89% for FF and FD). In addition, 25%, 62.5% and 75% of injected couples gave spawning that hatched in DD, FF and FD groups respectively. Larval quality parameters were not significantly different between treatments. The results indicate that overall quality of reproduction was higher in FF and FD treatments than in DD. It suggests that the dry feed used was not totally adequate for pikeperch reproduction. Relations between breeder reproductive performances and the feed compositions are discussed. [less ▲]Detailed reference viewed: 60 (10 ULg)