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See detailTHE BCCM/ULC CULTURE COLLECTION TO CONSERVE AND STUDY THE DIVERSITY OF (SUB)POLAR CYANOBACTERIA
Wilmotte, Annick ULg; Renard, Marine ULg; Kleinteich, Julia et al

Poster (2014, September)

The BCCM/ULC public collection of (sub)polar cyanobacteria is funded since 2011 by the Belgian Science Policy Office. A Quality Management System was implemented and is continuously improved since then ... [more ▼]

The BCCM/ULC public collection of (sub)polar cyanobacteria is funded since 2011 by the Belgian Science Policy Office. A Quality Management System was implemented and is continuously improved since then. An ISO9001 certificate was obtained for the public deposition and distribution of strains, as part of the multi-site certification for the BCCM consortium. BCCM/ULC is currently holding 134 cyanobacterial strains and the catalogue is available on http://bccm.belspo.be/catalogues/ulc-catalogue-search. Continuous maintenance of living cultures, some of which are also cryopreserved, ensure the preservation and the possibility to rapidly deliver strains to clients for fundamental and applied research. The collection includes 113 (sub)polar strains. In such extreme environments, cyanobacteria are important phototrophs and primary producers in terrestrial and freshwater ecosystems. The purpose of this collection is to gather a representative portion of the cyanobacterial diversity with different ecological origins (limnetic microbial mats, soil crusts, cryoconites, endoliths, etc.) and make it available for researchers to study the taxonomy, evolution, adaptations to environmental conditions, and genomic make-up. Thus, 102 cyanobacterial strains were isolated from the three main biogeographic zones of the Antarctic continent. In addition, 7 strains were isolated in Arctic biotopes and 4 from Siberian lakes. The molecular characterization is underway, on the basis of 16S rRNA and ITS sequences. Moreover, a Multilocus Sequence Analysis is tested on diverse strains to improve their systematics. The diversity encompasses the three main cyanobacterial orders: Chroococcales, Oscillatoriales and Nostocales. In addition, cyanobacteria are known to produce a range of secondary metabolites (e.g. alkaloides, cyclic and linear peptides, polyketides) with different bioactive properties (e.g. antibiotic, antiviral, antifungal, anticancer). The potential of the polar strains to produce cyanotoxins is currently studied by ELISA and the detection by PCR of genes involved in their production. Due to the geographic isolation and the strong environmental stressors of the habitat, the exploration of these metabolites in Antarctic cyanobacterial strains seems promising for biotechnology or biomedical applications. [less ▲]

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See detailEx-situ’ conservation of Antarctic cyanobacteria : a culture collection to explore diversity and bioactivity
Wilmotte, Annick ULg; Renard, Marine ULg; Kleinteich, J et al

Poster (2014, August)

Cyanobacteria appear as the dominant phototrophs in Antarctic terrestrial and freshwater ecosystems. Since 2011, the Belgian Science Policy Office has funded the BCCM/ULC public collection of (sub)polar ... [more ▼]

Cyanobacteria appear as the dominant phototrophs in Antarctic terrestrial and freshwater ecosystems. Since 2011, the Belgian Science Policy Office has funded the BCCM/ULC public collection of (sub)polar cyanobacteria. It is currently holding 102 Antarctic cyanobacterial strains and the catalogue is available on http://bccm.belspo.be/db/ulc_search_form.php. A Quality Management System was implemented and an ISO9001 certificate was obtained for the public deposition and distribution of strains. The strains are kept as living cultures, and their cryopreservation is in progress. The Antarctic cyanobacterial strains were isolated from samples of the three main biogeographic provinces. The purpose of this public collection is to gather a representative portion of the cyanobacterial diversity with different ecological origins (limnetic microbial mats, soil crusts, cryoconites, endoliths, etc.) and make it available for researchers to study the diversity, evolution, adaptations to the environmental conditions, and genomic make-up. Three cyanobacterial orders are represented: Chroococcales, Oscillatoriales and Nostocales. This is particularly important in view of the emerging use of metagenomic approaches on environmental samples, where the comparisons with the genome sequences from well-defined strains is very useful. They could also serve as references for compounds such as fatty acids and pigments. In addition, cyanobacteria produce a range of secondary metabolites (e.g. alkaloides, cyclic and linear peptides, polyketides) with different bioactive potential (e.g. antibiotic, antiviral, anticancer, cytotoxic, genotoxic). Bioassays have shown antifungal activities of the cell extracts of strains Plectolyngbya hodgsonii ULC009 and Phormidium priestleyi ULC026. Due to the geographic isolation and the strong environmental stressors of the habitat, the exploration of these metabolites in Antarctic cyanobacterial strains seems especially promising for biotechnology or biomedical applications. In summary, the BCCM/ULC public collection could serve as a general reference for Antarctic cyanobacteria with multiple applications, as well as a resource for novel bioactive compounds. [less ▲]

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See detailBCCM/ULC, a culture collection to explore diversity and bioactivity of (sub)polar cyanobacteria
Wilmotte, Annick ULg; Renard, Marine ULg; Kleinteich, Julia et al

Poster (2014, June)

The BCCM/ULC public collection of (sub)polar cyanobacteria is funded since 2011 by the Belgian Science Policy Office. A Quality Management System was implemented and is continuously improved since then ... [more ▼]

The BCCM/ULC public collection of (sub)polar cyanobacteria is funded since 2011 by the Belgian Science Policy Office. A Quality Management System was implemented and is continuously improved since then. An ISO9001 certificate was obtained for the public deposition and distribution of strains, as part of the multi-site certification for the BCCM consortium. BCCM/ULC is currently holding 134 cyanobacterial strains and the catalogue is available on http://bccm.belspo.be/catalogues/ulc-catalogue-search Continuous maintenance of living cultures, some of which are also cryopreserved, ensure the preservation and the possibility to rapidly deliver strains to clients for fundamental and applied research. The collection includes 113 (sub)polar strains. In such extreme environments, cyanobacteria are important phototrophs and primary producers in terrestrial and freshwater ecosystems. The purpose of this public collection is to gather a representative portion of the cyanobacterial diversity with different ecological origins (limnetic microbial mats, soil crusts, cryoconites, endoliths, etc.) and make it available for researchers to study the taxonomy, evolution, adaptations to environmental conditions, and genomic make-up. Thus, 102 cyanobacterial strains were isolated from the three main biogeographic zones of the Antarctic continent. In addition, 7 strains were isolated in Arctic biotopes and 4 from Siberian lakes. The molecular characterization is underway, on the basis of 16S rRNA and ITS sequences. The diversity encompasses the three main cyanobacterial orders: Chroococcales, Oscillatoriales and Nostocales. This is particularly important in view of the emerging use of metagenomic approaches on environmental samples, where the comparisons with the genome sequences from well-defined strains is very useful. In addition, cyanobacteria are known to produce a range of secondary metabolites (e.g. alkaloides, cyclic and linear peptides, polyketides) with different bioactive potential (e.g. antibiotic, antiviral, antifungal, anticancer, cytotoxic). The potential of the polar strains to produce cyanotoxins is currently studied by ELISA and the detection by PCR of genes involved in their production. Due to the geographic isolation and the strong environmental stressors of the habitat, the exploration of these metabolites in Antarctic cyanobacterial strains seems especially promising for biotechnology or biomedical applications. In summary, the BCCM/ULC public collection could serve as a general reference for (sub)polar cyanobacteria with multiple applications (pigments, UV screens, …), as well as a resource for novel bioactive compounds [less ▲]

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See detailStudy and conservation of Antarctic cyanobacterial diversity in the BCCM/ULC collection
Wilmotte, Annick ULg; Renard, Marine; Ould Amer, Yasmine et al

Poster (2013, July)

The BCCM/ULC public collection presently includes 72 Antarctic cyanobacterial strains and its catalogue is available on http://bccm.belspo.be/db/ulc_search_form.php. An ISO9001 certificate was obtained ... [more ▼]

The BCCM/ULC public collection presently includes 72 Antarctic cyanobacterial strains and its catalogue is available on http://bccm.belspo.be/db/ulc_search_form.php. An ISO9001 certificate was obtained for the public deposition and distribution of strains. The purpose of this collection is to gather a representative sample of Antarctic cyanobacterial diversity from different biotopes (limnetic microbial mats, soil crusts, cryoconites, endoliths, etc.) and make it available for researchers to study the diversity, evolution, physiology, and genomic make-up. This is particularly important in view of the emerging use of metagenomic approaches on environmental samples, where the comparison with well-defined strain genome sequences can be very useful. [less ▲]

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See detailElucidation of the gas vesicle gene clusters in cyanobacteria of the genus Arthrospira (Oscillatoriales, Cyanophyta) and correlation with ITS phylogeny
Miklaszewska, Magdalena; Waleron, Malgorzata; Morin, Nicolas et al

in European Journal of Phycology (2012), 47

The genus Arthrospira comprises filamentous cyanobacteria in which the trichomes form an open helix and contain gas vacuoles. The gas vesicle gene cluster of five Arthrospira strains was amplified by PCR ... [more ▼]

The genus Arthrospira comprises filamentous cyanobacteria in which the trichomes form an open helix and contain gas vacuoles. The gas vesicle gene cluster of five Arthrospira strains was amplified by PCR and sequenced. The genes are organized in one operon, in the order gvpA1–gvpC1–gvpA2–gvpC2–gvpA3–gvpC3–gvpN. In Arthrospira sp. strain PCC 8005, the genes gvpJ, gvpK, gvpV and gvpW were also identified. Each of the three copies of gvpA encodes a protein of 71 amino acids. In the case of gvpC, there are two different length variants. Each of the two shorter genes, gvpC1 and gvpC2, encodes a putative protein of 151 amino acids, while the longer one, gvpC3, codes for a putative protein of 284 residues. The amino acid sequences of GvpC1 and GvpC2 are identical to the N-terminal part of GvpC3. In spite of the presence of stop codons downstream of gvpC1 and gvpC2, the deduced amino acid sequences in these regions are highly similar to the C-terminal part of GvpC3 (residues 160 to 229). The GvpC1, GvpC2 and GvpC3 proteins contain contiguous repeats of 33 amino acids as previously reported for other cyanobacteria. The sequences of the gvpA1, gvpC1, gvpA2 and gvpC2 genes were not found in the genome data of Arthrospira sp. PCC 8005, A. maxima CS-328, and A. platensis NIES-39 as a result of incomplete assembly. The genes gvpN and gvpJ located downstream of gvpC3, encode putative proteins of 394 and 127 amino acids, respectively. The deduced amino acid sequences of gvpK, gvpV and gvpW contain 151, 112 and 227 residues, respectively. The analysis of gvp sequences of five strains of Arthrospira revealed the presence of polymorphic positions, which distinguished the strains in agreement with their previous assignments to ITS clusters I and II. This is the first report of gvp genes in members of the genus Arthrospira. [less ▲]

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See detailA collection of polar cyanobacteria to contribute to the inventory of the biodiversity and discover the biotechnological potential
Wilmotte, Annick ULg; Waleron, Kzryzstof; Waleron, Malgorzata et al

Poster (2011, February)

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See detailGenome Sequence of the Edible Cyanobacterium Arthrospira sp. PCC 8005
Janssen, Paul; Morin, Nicolas; Mergeay, Max et al

in Journal of Bacteriology (2010), 192(9), 24652466

We determined the genome sequence of Arthrospira sp. PCC 8005, a cyanobacterial strain of great interest to the European Space Agency for its nutritive value and oxygenic properties in the Micro ... [more ▼]

We determined the genome sequence of Arthrospira sp. PCC 8005, a cyanobacterial strain of great interest to the European Space Agency for its nutritive value and oxygenic properties in the Micro-Ecological Life Support System Alternative (MELiSSA) biological life support system for long-term manned missions into space. [less ▲]

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