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See detailAzoxystrobin and epoximazole sensitivity profiles of Mycosphaerella graminicola populations from the Grand-Duchy of Luxemburg in 2007 and 2008
Vrancken, Carine; Dubos, Tiphaine; El Jarroudi, Moussa ULg et al

Poster (2010, December 07)

Mycosphaerella graminicola strains were isolated from symptomatic winter wheat (Triticum aestivum) and spelt (Triticum spelta) leaves sampled across the Grand-Duchy of Luxembourg. In total, 484 strains ... [more ▼]

Mycosphaerella graminicola strains were isolated from symptomatic winter wheat (Triticum aestivum) and spelt (Triticum spelta) leaves sampled across the Grand-Duchy of Luxembourg. In total, 484 strains were isolated from winter wheat, and 31 from spelt leaves. The sensitivity profiles of these strains towards azoxystrobin (Amistar®, Syngenta Agro GmbH, Germany) and against epoxiconazole (Opus®, BASF Belgium NV/S A, Brussels, Belgium) were assessed in microplates, allowing to test 10 different concentrations for each active substance (0, 0.00316, 0.01, 0.0316, 0.1, 0.316, 1, 3.16, 10 and 31.6μg/mL). 100μ g/mL of salicylhydroxamic acid (SHAM) was also added to the medium, in order to prevent the strains from using alternative respiration. Results of the sensitivity tests for azoxystrobin showed a dose-dependent decrease of in vitro growth for all the strains. During both years, strains could be divided into two distinct sub-populations in relation to their azoxystrobin sensitivity, thus forming a bimodal distribution commonly reported to be associated with a single gene-mediated resistance. For wheat, the dominant sub-population was the one showing full resistance to azoxystrobin. The results of the sensitivity tests to epoxiconazole in liquid media showed a dose-dependent decrease of fungal growth for all the strains tested. The EC50 values followed an unimodal distribution. When the sensitivity distribution of the strains collected in 2008 (n=379) was compared to that observed in 2007, although the populations have remained unimodal, a minor shift towards less sensitive populations was observed. Compared to strains originating from wheat, the spelt strains were more sensitive towards both fungicides (p ≤ 0.001). [less ▲]

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See detailFusarium head blight and associated mycotoxin occurrence on winter wheat in Luxembourg in 2007/2008
Giraud, Frédéric; Pasquali, Matias; El Jarroudi, Moussa ULg et al

in Food Additives & Contaminants (2010), 27

Fusarium head blight (FHB) is among the major causes of reduced quality in winter wheat and its products. In addition, the causal fungi produce a variety of toxins. A relatively high FHB infection rate in ... [more ▼]

Fusarium head blight (FHB) is among the major causes of reduced quality in winter wheat and its products. In addition, the causal fungi produce a variety of toxins. A relatively high FHB infection rate in winter wheat was observed in 2007 and 2008 in Luxembourg. A fusariotoxin survey was carried out in 17 different geographical locations. Three groups of Fusarium mycotoxins (trichothecenes A and B and zearalenone) were analysed by a multi-detection HPLC–MS/MS method. Fusarium strains were also investigated by morphological and molecular methods. In addition, questionnaires relating to cultural practices were sent to the farmers managing the 17 fields investigated. FHB prevalence ranged from 0.3 to 65.8% (mean: 8.5%) in 2007 and from 0 to 24.5% (mean: 8.3%) in 2008. Results of morphological and molecular identification showed that the most common species isolated from diseased wheat spikes was F. graminearum (33.1%), followed by F. avenaceum (20.3%) and F. poae (17.8%). The chemical analysis revealed that 75% of the investigated fields were contaminated by deoxynivalenol (DON, range 0–8111 mg/kg). The preceding crop was highly and significantly correlated to the number of grains infected and had a significant impact on disease prevalence ( p¼0.025 and 0.017, respectively, Fisher’s F-test). A trend was found for maize as the preceding crop ( p¼0.084, Tukey’s test) to predict the amount of DON in the fields. This is the first report on the occurrence of DON and ZON in naturally infected wheat grains sampled from Luxembourg. [less ▲]

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See detailFirst Report of Wheat Leaf Rust in the Grand Duchy of Luxembourg and the Progress of its Appearance over the 2003–2008 Period
El Jarroudi, Moussa ULg; Giraud, Frédéric; Vrancken, Carine et al

in Plant Disease (2009), 93

Wheat leaf rust caused by Puccinia triticina Eriks. was identified for the first time in 2000 in the Grand Duchy of Luxembourg on the basis of orange-to-brown, round-to-ovoid, erumpent uredinia (1 to 1.5 ... [more ▼]

Wheat leaf rust caused by Puccinia triticina Eriks. was identified for the first time in 2000 in the Grand Duchy of Luxembourg on the basis of orange-to-brown, round-to-ovoid, erumpent uredinia (1 to 1.5 mm in diameter) scattered on the upper and lower leaf surfaces and producing orange-brown urediniospores that are subgloboid, approximately 20 μm in diameter, and with up to eight germ pore scattered in thick, echinulate walls. In a second phase, wheat was monitored weekly (starting from Zadoks growth stage 30, pseudo stem erection) during the 2003–2008 cropping seasons for wheat leaf rust. Disease severity (percentage of leaf area with symptoms) was recorded in four, replicated field experiments located in three villages (Diekirch District: Reuler; and Grevenmacher District: Burmerange and Christnach), which are representative of the different agroclimatological zones of Luxembourg. A significant difference in severity was observed between the sites (P < 0.01) and the years (P < 0.05). Over the 6-year period, Burmerange and Reuler consistently showed the highest and lowest disease severity, respectively. In 2003 and 2007, Burmerange (a southern site with the highest average spring temperatures of 13.6 and 14.0°C, respectively) showed the highest disease severity with 66 and 57%, respectively, whereas the lowest severity (<1% for both years) was observed in the north at Reuler (site with the lowest average spring temperatures of 12.0 and 12.4°C, respectively). Christnach, located midway between Reuler and Burmerange, showed an intermediate disease severity with 7% (2003) and 22% (2007). The disease appeared at growth stages 77 (late milk) and 87 (hard dough) in the period 2003–2005, but at an earlier stage (45, boots swollen) for 2006–2008 (P < 0.001). In 2005, low severity was recorded due to a severe drought during May, June, and July. A reason for this earlier appearance of leaf rust occurrences in the two districts may be related to an increase in the average spring temperature (average March to May temperature for Luxembourg was 8.3°C for the 1971–2000 period, 9.5°C for the 2003–2005 period, 9.9°C for the 2006–2008 period, 2007 was exceptional with 11.9°C, P < 0.01). In the past, cereal disease management strategies were oriented toward the control of predominant and yield-reducing diseases such as that caused by Septoria tritici Desm. Because the succession of mild winters and warm springs during the last 5 years allowed the early occurrence and the fast development of wheat leaf rust in the Grand Duchy of Luxembourg, it is advisable to take this disease into account in fungicide application schemes. [less ▲]

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See detailFusarioses sur blé d'hiver au Grand-Duché de Luxembourg
Giraud, Frédéric; Pasquali, Matias; Vrancken, Carine et al

in Phytoma - la Défense des Végétaux (2009), 622-623

Au Grand-Duché de Luxembourg, la campagne 2006-2007 a été caractérisée par un hiver relativement doux et un printemps pluvieux. Ces conditions météorologiques ont permis le développement de nombreuses ... [more ▼]

Au Grand-Duché de Luxembourg, la campagne 2006-2007 a été caractérisée par un hiver relativement doux et un printemps pluvieux. Ces conditions météorologiques ont permis le développement de nombreuses maladies cryptogamiques sur le blé d’hiver, dont la fusariose. Cette maladie a été suivie sur 17 sites couvrant le G-D de Luxembourg par des mesures de prévalence (pourcentage moyen d’épis visuellement infectés) et de sévérité (pourcentage moyen d’épillets atteints par épi). Des valeurs moyennes de 8,9± +/- 15,5 % pour la prévalence et de 21,0± +/- 17,8 % pour la sévérité ont été enregistrées avec des différences significatives entre les cantons nord et sud pour la sévérité. Plus de 600 souches isolées des 17 sites ont été identifiées suivant des critères morphologiques et l’emploi d’outils moléculaires. 5 espèces semblent associées à la fusariose : Fusarium graminearum (23,5 %), F. culmorum (12,8 %), F. poae (19,4 %) (3 espèces potentiellement productrices de mycotoxines), F. avenaceum (20 %) et Microdochium nivale (18,7 %). Cette étude est la première du genre à avoir été faite au Grand-Duché de Luxembourg. [less ▲]

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See detailFirst Report of Fusarium Head Blight (FHB) on Winter Wheat in the Grand Duchy of Luxembourg
Giraud, Frédéric; Vrancken, Carine; Delfosse, Philippe et al

in Plant Disease (2008), 92

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See detailLe rumex à feuilles obtuses dans les systèmes herbagers : importance de la problématique, lutte chimique et méthodes alternatives
Stilmant, Didier ULg; Bodson, Bernard ULg; Knoden, D. et al

in Fourrages (2008), 192

Parmi les questions relatives à l'entretien des prairies, la lutte contre le rumex est une préoccupation fréquente, du fait de la vigueur et de la capacité d’adaptation considérables de la plante et de la ... [more ▼]

Parmi les questions relatives à l'entretien des prairies, la lutte contre le rumex est une préoccupation fréquente, du fait de la vigueur et de la capacité d’adaptation considérables de la plante et de la difficulté de la lutte contre cette adventice invasive dans des couverts complexes... Une enquête réalisée en région wallonne auprès d'exploitants agricoles et portant sur leurs pratiques de gestion et d'entretien des prairies montre l’importance de la pression exercée par Rumex obtusifolius L. qui représente un problème pour 40% d'entre eux ; la complémentation et l'épandage de lisier et de fumier de bovins sont corrélés avec la difficulté exprimée par les éleveurs. Une revue complète des traitements chimiques est effectuée selon la nature du couvert et l'âge de la prairie. Diverses méthodes de lutte alternatives sont également présentées (faux semis, type de travail du sol, gestion du pâturage…) ainsi que leur efficacité sur les jeunes plantules et sur les repousses de plantes de rumex. [less ▲]

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See detailA special year for Fusarium Head Blight and associated mycotoxins in Luxembourg
Giraud, Frédéric; Vrancken, Carine; El Jarroudi, Moussa ULg et al

in Toxicology Letters (2008), 180

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See detailLe rumex à feuilles obtuses dans les systèmes herbagers: importance de la problématique, lutte chimique et méthodes alternatives
Stilmant, Didier ULg; Knoden, D.; Bodson, Bernard ULg et al

in Fourrages (2007), (192), 477-493

Une enquête réalisée en région wallonne auprès d'exploitants agricoles et portant sur leurs pratiques de gestion et d'entretien des prairies montre l'importance de la pression exercée par rumex ... [more ▼]

Une enquête réalisée en région wallonne auprès d'exploitants agricoles et portant sur leurs pratiques de gestion et d'entretien des prairies montre l'importance de la pression exercée par rumex obtusifolius L. qui représente un problème pour 40 % d'entre eux; la complémentation et l'épandage de lisier et de fumier de bovins sont corrélés avec la difficulté exprimée par les éleveurs. Une revue complète des traitements chimiques est effectuée selon la nature du couvert et l'âge de la prairie. Diverses méthodes de lutte alternatives sont également présentées (faux semis, type de travail du sol, gestion du pâturage, ...) ainsi que leur efficacité sur les jeunes plantules et sur les repousses de plantes de rumex. [less ▲]

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