References of "Verstegen, John"
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See detailEvaluation of the urodynamic and hemodynamic effects of orally administered phenylpropanolamine and ephedrine in female dogs.
Carofiglio, Francesca; Hamaide, Annick ULg; Farnir, Frédéric ULg et al

in American Journal of Veterinary Research (2006), 67(4), 723-730

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See detailUrodynamic and morphologic changes in the lower portion of the urogenital tract after administration of estriol alone and in combination with phenylpropanolamine in sexually intact and spayed female dogs
Hamaide, Annick ULg; Grand, Jean-Guillaume; Farnir, Frédéric ULg et al

in American Journal of Veterinary Research (2006), 67(5), 901-908

OBJECTIVE: To compare the urodynamic and morphologic effects of the administration of estriol alone and in combination with phenylpropanolamine on the lower portion of the urogenital tract in female dogs ... [more ▼]

OBJECTIVE: To compare the urodynamic and morphologic effects of the administration of estriol alone and in combination with phenylpropanolamine on the lower portion of the urogenital tract in female dogs. ANIMALS: 3 sexually intact and 3 spayed female Beagles without urinary incontinence. PROCEDURE: Dogs received estriol (2 mg, PO) once daily for 7 days followed by estriol (2 mg, PO) and phenylpropanolamine (1.5 mg/kg, PO) once daily for 7 days. Urethral pressure profilometry, diuresis cystometry, and vaginourethrography were performed before treatment (day 0) and at days 7 and 14. The maximum urethral pressure (MUP) and closure pressure (MUCP), urethral functional and anatomic profile lengths, integrated pressure (IP), plateau, distance before MUP, maximum meatus pressure, threshold pressure, threshold volume, compliance, urethral length, and vaginal length and width were measured. RESULTS: Before treatment, no urodynamic differences were observed between the 2 groups; however, vaginal length and width were significantly shorter in spayed dogs. Compared with day 0 values, estriol treatment significantly increased MUP, MUCP, and IP values at day 7, but at day 14, this effect decreased despite phenylpropanolamine administration. No morphologic changes from baseline were detected after either treatment in any dog. CONCLUSIONS AND CLINICAL RELEVANCE: Data suggest that estriol mainly acts on the urethral sphincter mechanism by increasing urethral resistance in sexually intact and spayed female dogs without urinary incontinence. Administration of estriol and phenylpropanolamine did not increase the urethral resistance more than estriol alone. The urodynamic effects of estriol in female dogs with urinary incontinence remain to be elucidated [less ▲]

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See detailCanine herpesvirus-1 (CHV-1): clinical, serological and virological patterns in breeding colonies
Ronsse, Veerle; Verstegen, John; Thiry, Etienne ULg et al

in Theriogenology (2005), 64(1), 61-74

Canine herpesvirus-1 (CHV-1) is presumed to be enzootic in the dog population and is associated with reproductive disorders and neonatal mortality. To advise dog breeders towards an effective management ... [more ▼]

Canine herpesvirus-1 (CHV-1) is presumed to be enzootic in the dog population and is associated with reproductive disorders and neonatal mortality. To advise dog breeders towards an effective management of CHV-1 infected colonies, 27 breeding bitches were studied during one reproductive cycle in field conditions: the effect of cycle stage, kennel size, initial antibody titre, mating and gestation on serologic and viral excretion patterns was evaluated, while the association between reproductive disorders and CHV-1 antibody titres and viral excretion was also analysed. All initially seronegative bitches seroconverted, while 40% of the initially seropositive bitches became seronegative at one or two occasions. No difference in antibody patterns was observed between mated and unmated bitches. Of the mated bitches, 40% experienced infertility, foetal resorption or mummification. No difference in antibody patterns was observed depending on the occurrence of reproductive disorders even if a decrease in antibody titres during early or late-di-oestrus was often present. Significantly higher titres were observed at all cycle stages in large kennels. None of the vaginal and nasal samples or buffy coats tested positive for CHV-1 DNA. The mixed image of clinical and subclinical carriage in this study demonstrated CHV-1 has a complex and difficult to predict clinical behavior. Preventive management with vaccination of reproducing bitches in kennels with reproductive disorders should therefore be advised. (c) 2004 Elsevier Inc. All rights reserved. [less ▲]

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See detailInfluence of the estrous cycle on urodynamic and morphometric measurements of the lower portion of the urogenital tract in dogs
Hamaide, Annick ULg; Verstegen, John; Snaps, Frédéric ULg et al

in American Journal of Veterinary Research (2005), 66(6), 1075-1083

OBJECTIVES: To compare the values of the urodynamic parameters of the lower portion of the urinary tract and vaginourethral measurements obtained during the phases of the estrous cycle in dogs and ... [more ▼]

OBJECTIVES: To compare the values of the urodynamic parameters of the lower portion of the urinary tract and vaginourethral measurements obtained during the phases of the estrous cycle in dogs and determine possible functional or anatomic modifications of the lower portion of the urinary tract associated with those phases. ANIMALS: 7 adult female Beagles. PROCEDURE: Urethral pressure profilometry, diuresis cystometry, and vaginourethrography were performed in each dog during proestrus; estrus; early, mid, and late diestrus; and early and late anestrus. The maximum urethral pressure (MUP), maximum urethral closure pressure (MUCP), urethral functional and anatomic profile lengths (UFPL and UAPL, respectively), integrated pressure, threshold pressure, threshold volume, compliance, urethral length, and vaginal length and width were measured. RESULTS: For all measurements, significant interindividual variation was detected. Integrated and threshold pressures, APL, and each morphometric value significantly increased from late anestrus to proestrus. Compared with other phases, MUP, MUCP, and integrated pressure values were significantly lower in estrus and early diestrus; UAPL and UFPL values were significantly lower in late diestrus. At each cycle phase in old dogs, MUP, MUCP, threshold pressure, and vaginal length and width were significantly lower (except in proestrus for vaginal measurements) and threshold volume and compliance values were significantly higher, compared with middle-aged dogs. CONCLUSIONS AND CLINICAL RELEVANCE: Urodynamic and morphometric measurements of the lower portion of the urogenital tract are affected by the changes in hormonal balance that occur during the estrous cycle. In sexually intact female dogs, estrous phase determination is important for the interpretation of urodynamic data. [less ▲]

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See detailUrethral sphincter mechanism incompetence in the bitch. Review of the literature
Hamaide, Annick ULg; Balligand, Marc; Verstegen, John

in Annales de Médecine Vétérinaire (2005), 149(2), 75-92

Urethral sphincter mechanism incompetence is characterized by unvoluntary urine leakage, worsening when the animal is recumbent or asleep, or during excitement. It mainly affects large breed dogs, and ... [more ▼]

Urethral sphincter mechanism incompetence is characterized by unvoluntary urine leakage, worsening when the animal is recumbent or asleep, or during excitement. It mainly affects large breed dogs, and mostly middle-aged spayed bitches. The aetiology of this condition is multifactorial and predisposing factors include a decrease urethral tone, an intrapelvic bladder neck position, a short urethra, sterilisation, obesity, and age. Diagnostic workup should include a vaginourethrography to identify possible vaginal abnormalities and to visualize the urethra and the position of the bladder neck, and urethral profilometry to demonstrate a decrease in maximal urethral pressure and functional profile length. Medical treatment with oestriol is an option. This short-acting oestrogen does not exhibit oestrogenic side effects. Unfortunately, its success rate is only of 65 % of complete continence. The a- adrenergic agents ( phenylpropanolamine and ephedrine) are more efficacious, with a success rate reaching 75 to 90 %, with very few side effects. In absence of response to the treatment, or if the animal becomes refractory, a colposuspension can be recommended, with a fairly good prognosis. [less ▲]

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See detailValidation and comparison of the use of diuresis cystometry and retrograde filling cystometry at various infusion rates in female Beagle dogs
Hamaide, Annick ULg; Verstegen, John; Snaps, Frédéric ULg et al

in American Journal of Veterinary Research (2003), 64(5), 574-579

OBJECTIVES: To compare retrograde filling cystometry at infusion rates of 5, 10, and 20 mL/min with diuresis cystometry for determination of an appropriate infusion rate and to confirm the reproducibility ... [more ▼]

OBJECTIVES: To compare retrograde filling cystometry at infusion rates of 5, 10, and 20 mL/min with diuresis cystometry for determination of an appropriate infusion rate and to confirm the reproducibility of measurements obtained by urethral pressure profilometry (UPP) and cystometry in female Beagles. ANIMALS: Adult female Beagles. PROCEDURE: Successive UPP and cystometry were performed by use of a water perfusion catheter on dogs anesthetized with propofol. Dogs randomly underwent each of the following at 1-week intervals: retrograde filling cystometry at 5, 10, and 20 mL/min, and diuresis cystometry. The maximum urethral pressure and closure pressure, functional and anatomic profile lengths, threshold pressure, threshold volume, and compliance were measured. RESULTS: For each UPP variable, significant differences were found among dogs, but no significant differences were found in intra- or interstudy measurements for individual dogs. For retrograde filling cystometry, threshold pressure was not significantly different between a 5 and 10 mL/min infusion rate. Threshold pressure was significantly higher during retrograde filling cystometry at 20 mL/min, compared with 5 and 10 mL/min, and was associated with bladder wall damages. Threshold pressure was significantly lower during diuresis cystometry, compared with retrograde filling cystometries. Threshold volume and compliance were not significantly different among retrograde filling cystometries but were significantly higher during diuresis cystometry. CONCLUSIONS AND CLINICAL RELEVANCE: Retrograde filling cystometry at 20 mL/min leads to unacceptable sudden increase in threshold bladder pressure. Retrograde filling cystometry at 10 mL/min can be recommended in a clinical setting, shortening the anesthesia time. However, diuresis cystometry approximates physiologic bladder filling most accurately [less ▲]

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See detailRecommended methods for euthanasia
Marlier, Didier ULg; Verstegen, John; Serteyn, Didier ULg et al

Scientific conference (2000)

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See detailLocalisation immunocytochimique de l'alphamelanotropine dans le placenta bovin
Verstegen, John; Fellmann, Dominique; Beckers, Jean-François ULg

in Comptes Rendus de l'Académie des Sciences. Série III, Sciences de la Vie (1985), 301(III-2), 41-46

The immunocytochemical study of the bovine placenta has demonstrated the presence of cells containing alphamelanotropic hormones, in the decidual epithelium, since the 4th month of pregnancy. The number ... [more ▼]

The immunocytochemical study of the bovine placenta has demonstrated the presence of cells containing alphamelanotropic hormones, in the decidual epithelium, since the 4th month of pregnancy. The number and the staining intensity of those cells grow up with the placental development. Since the 6th month, we observed the immunoreactional presence of cells in the foetal chorionic epithelium. [less ▲]

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See detailDosage radioimmunologique de l'alpha-MSH: Elaboration d'un antisérum hautement spécifique: application chez le rat
Verstegen, John; Ghaenen, G.; Dessy, Cécile ULg et al

in Annales de Recherches Vétérinaires = Annals of Veterinary Research (1985), 16(4), 313-320

A highly specific and sensitive RIA for alpha-MSH has been developed. Its main characteristic is the possibility to mesure the alpha-MSH in the whole plasma without extraction. The double incubation ... [more ▼]

A highly specific and sensitive RIA for alpha-MSH has been developed. Its main characteristic is the possibility to mesure the alpha-MSH in the whole plasma without extraction. The double incubation technics has been utilized. It has been possible by the utilisation of highly specific and affin antiserum. [less ▲]

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