References of "Verdin, Emeline"
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See detailInfluence of medium composition on oxygen transfer rate in animal cell culture
Toye, Dominique ULg; Galifi, A.; Salmon, Thierry ULg et al

in Canadian Journal of Chemical Engineering (2010), 88(4), 671-676

Experiments were conducted in a 0.25m diameter bubble column to investigate the effect of medium composition on oxygen transfer rate. Aqueous solutions, the composition of which mimics a mammalian cell ... [more ▼]

Experiments were conducted in a 0.25m diameter bubble column to investigate the effect of medium composition on oxygen transfer rate. Aqueous solutions, the composition of which mimics a mammalian cell culture medium, are used. The effect on oxygen transfer rate of additives used to protect cells against local hydrodynamic stresses induced by bubble coalescence and bursting is addressed, in the range of operating conditions (aeration rates) met in animal cell cultures. The mass transfer coefficient, the liquid viscosity and surface tension, and the bubble size distribution are measured as a function of liquid composition and of gas superficial velocity, allowing to decouple the effects of the different additive on kL and on a. [less ▲]

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See detailSimulation de l’écoulement dans des filtres à charbons actifs par la méthodologie des réseaux de Boltzmann
Verdin, Emeline ULg; Beugre, Djomice Antoine ULg; Ania, Conchi et al

in SFGP (Ed.) Récents Progrès en Génie des Procédés - N°98 - A la croisée des sciences et des cultures pour relever les défis industriels du XXIème siècle - Actes du 12ème Congrès de la Société Française de Génie des Procédés, Marseille, France, 14-16 octobre 2009 (2009)

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See detailInfluence of medium composition on oxygen transfer rate in animal cell culture
Toye, Dominique ULg; Galifi, Alain; Salmon, Thierry ULg et al

in Proceedings of 8th World Congress on Chemical Engineering (2009)

Experiments were conducted in a 0.25 m diameter bubble column to investigate the effect of animal cell culture medium composition on oxygen transfer rate. Air is used as the dispersed phase. The gas ... [more ▼]

Experiments were conducted in a 0.25 m diameter bubble column to investigate the effect of animal cell culture medium composition on oxygen transfer rate. Air is used as the dispersed phase. The gas superficial velocity is varied between 0.4 and 2 cm/s (aeration rate ranging between 0.05 and 0.25 vvm) and the bubble column is thus operated in the homogenous regime. Aqueous solutions the composition (electrolyte, protein concentrations) of which mimics a mammalian cell culture medium) are used as the continuous phase. In particular, the effect on oxygen transfer rate of additive such as PVP, Pluronic F-68 which are usually used to protect cells against local hydrodynamic stresses induced by bubble coalescence and bursting is addressed. For each composition, the mass transfer coefficient is measured by the "gas in – gas out" method using a fast response dissolved oxygen probe. Its increase with gas velocity is measured. The liquid viscosity and surface tension are experimentally determined as a function of culture medium composition. Bubble size distribution is measured at different scales using three experimental techniques: gas disengagement technique, two dual optical probes and photography, which lead to results in good qualitative agreement one with each other. The integrated analysis of these data allows to decouple the effects of the different additive on coefficient kL and on the interfacial area, a. [less ▲]

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See detailSubtype Selective Substrates For Histone Deacetylases
Heltweg, B.; Dequiedt, Franck ULg; Marshall, Bl. et al

in Journal of Medicinal Chemistry (2004), 47(21),

To probe the steric requirements for deacylation, we synthesized lysine-derived small molecule substrates and examined structure-reactivity relationships with various histone deacetylases. Rat liver ... [more ▼]

To probe the steric requirements for deacylation, we synthesized lysine-derived small molecule substrates and examined structure-reactivity relationships with various histone deacetylases. Rat liver, human HeLa, and human recombinant class I and II histone deacetylases (HDACs) as well as human recombinant NAD(+)-dependent SIRT1 (class III enzyme) were used in these studies. A benzyloxycarbonyl substituent on the alpha-amino group yielded the highest conversion rates. Replacing the epsilon-acetyl group with larger lipophilic acyl substituents led to a pronounced decrease in conversion by class I and II enzymes; the class III enzyme displayed a greater tolerance. Incubations with recombinant FLAG-tagged human HDACs 1, 3, and 6 showed a distinct subtype selectivity among small molecule substrates. The subtype selectivity of HDAC inhibitors could be predicted with these substrates and an easily obtainable mixture of HDAC subtypes. [less ▲]

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See detailInsulin oscillations per se do not affect glucose turnover parameters in normal man.
Paolisso, G.; Scheen, André ULg; Verdin, Emeline ULg et al

in Journal of Clinical Endocrinology and Metabolism (1986), 63(2), 520-5

To compare the metabolic effects of pulsatile vs. continuous iv insulin infusion, normal men had two glucose-controlled iv glucose infusions using the Biostator for 260 min, during which endogenous ... [more ▼]

To compare the metabolic effects of pulsatile vs. continuous iv insulin infusion, normal men had two glucose-controlled iv glucose infusions using the Biostator for 260 min, during which endogenous pancreatic hormone secretion was inhibited by a somatostatin infusion and glucagon was replaced by continuous glucagon infusion. The two tests were performed at 1-week intervals, during which human insulin was infused either continuously at a constant rate of 0.2 mU kg-1 min-1 or in a pulsatile manner at a rate of 1.3 mU kg-1 min-1 with a switching on/off length of 2/11 min. Blood glucose levels and glucose infusion rates (GIR) were continuously monitored, and glucose turnover was estimated using a [3H]glucose infusion. In both tests, plasma C-peptide dropped markedly, whereas plasma glucagon levels were about twice basal values. Plasma insulin averaged 7 mU liter-1 during continuous infusion and oscillated between 1.5 and 35 mU liter-1 during pulsatile delivery. During the first 30-60 min of both tests, the glucose appearance rate and endogenous glucose production (EGP) increased, resulting in moderate hyperglycemia, which completely suppressed GIR. During the last 65 min, EGP declined, while the glucose disappearance rate and the glucose MCR increased, so that GIR increased progressively to maintain the blood glucose clamped at about 5 mmol liter-1. During this period, no significant differences were found between the two modes of insulin administration for any of the parameters studied. Thus, continuous and pulsatile insulin iv infusion, resulting in physiological peripheral plasma insulin levels, altered the glucose turnover parameters equally, in particular inhibiting EGP, which was stimulated by glucagon during the first part of the study, and stimulating peripheral glucose uptake at the end of the study period. [less ▲]

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