References of "Vandeplas, Sabrina"
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See detailSalmonella in chicken : Current and developing strategies to reduce contamination at farm level
Vandeplas, Sabrina ULg; Dubois Dauphin, Robin ULg; Beckers, Yves ULg et al

in Journal of Food Protection (2010), 73(4), 774-785

Salmonella sp. is a human pathogen that frequently infects poultry flocks. Consuming raw or undercooked contaminated poultry products can induce acute gastro-enteritis in human. Faced with the public ... [more ▼]

Salmonella sp. is a human pathogen that frequently infects poultry flocks. Consuming raw or undercooked contaminated poultry products can induce acute gastro-enteritis in human. Faced with the public health concern associated with salmonellosis, the European Union (EU) has established a European regulation forcing Member States (MS) to implement control programs aimed at reducing Salmonella prevalence in poultry production, especially at the primary production level. The purpose of the present review article is to summarise the current research and to suggest future developments in the area of Salmonella control in poultry, which may be of value to the industry in the coming years. The review will focus especially on preventive strategies that have been developed and that aim at reducing the incidence of Salmonella colonization in broiler chickens at farm level. Besides the usual preventive hygienic measures, different strategies have been investigated, like feed and drinking water acidification by organic acids and immune strategies based on passive and active immunity. Modulating the diet in terms of ingredient and nutrient composition with the intent of reducing the bird’s susceptibility to Salmonella has also been examined. Because in-ovo feeding has been shown to accelerate small intestinal development and to enhance the epithelial cell function, it could also be an efficient tool to control enteric pathogens. Microflora-modulating feed additives, like antibiotics, prebiotics, probiotics, and synbiotics, represent another field of investigation, whose success depends on the additive used. Finally, recent control methods, such as chlorate products and bacteriophages, have also been studied. [less ▲]

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See detailPrevalence and sources of Campylobacter spp. contamination in free-range broiler production in the southern part of Belgium
Vandeplas, Sabrina ULg; Dubois Dauphin, Robin ULg; Palm, Rodolphe ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14(2), 279-288

A one year epidemiological study was carried out between February 2005 and January 2006 in the southern part of Belgium to assess the Campylobacter prevalence in free-range broiler production. Three ... [more ▼]

A one year epidemiological study was carried out between February 2005 and January 2006 in the southern part of Belgium to assess the Campylobacter prevalence in free-range broiler production. Three successive broiler flocks from six Belgian farms were investigated for the presence of Campylobacter spp. during the rearing period. Each flock was visited four times, before and after the outdoor rearing period. During each visit, samples were taken in the broiler house (litter, cecal droppings, water-lines, feed, anteroom) as well as from the outer rearing environment (open-air range). The Campylobacter detection in all samples was carried out according to the ISO 10272 standard. Identification was based on colonial morphology, microscopic examination, and biochemical tests. PCR multiplex was used for genetic confirmation. Campylobacter jejuni was the main species isolated from all contaminated samples. Overall, mixed infections C. jejuni/C. coli represented 40.6%, while C. jejuni and C. coli represented 46.9% and 12.5% of chicken samples respectively. A 100% flock contamination was observed in the 6 farms during the summer/autumn period, whereas only 66.7% and 33.3% of the flocks became Campylobacter-positive in spring and winter respectively, at the end of the rearing period. Half of contaminated flocks were infected before chickens have access to the open-air range. Environmental samples, especially the open-air range soil, were found to be Campylobacter-positive before flock infection. The other potential sources of contamination were delivery tray, anteroom floor and water-lines. Other animal productions like cattle on the farm, no applied rodent control, no cleaning and disinfection of water-lines between flocks, no detergent used for cleansing and thinning were recorded as risk factors. In conclusion, the contact with the environment, particularly the access to an open-air range, appeared to be the major way of Campylobacter contamination of chickens in free-range broiler production. [less ▲]

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See detailEffect of the bacterial or fungal origin of exogenous xylanases supplemented to a wheat-based diet on performance of broiler chickens and nutrient digestibility of the diet
Vandeplas, Sabrina ULg; Dubois Dauphin, Robin ULg; Thonart, Philippe ULg et al

in Canadian Journal of Animal Science (2010), 90(2), 221-228

Two identical experiments were carried out to study the effects of four xylanases from bacterial or fungal origin supplemented to a wheat-based diet, on growth performance of broiler chickens and nutrient ... [more ▼]

Two identical experiments were carried out to study the effects of four xylanases from bacterial or fungal origin supplemented to a wheat-based diet, on growth performance of broiler chickens and nutrient digestibilities. Experimental treatments consisted of a control basal diet containing 600 g kg-1 wheat (C), and the basal diet supplemented with 0.1 g kg-1 Grindazyme G from Aspergillus niger (G), 0.1 g kg-1 Belfeed B1100MP from Bacillus subtilis (B), 0.1 g kg-1 Roxazyme G from Trichoderma viride (R), or 0.0125 g kg-1 of a xylanase from Aspergillus aculeatus (A). Each experimental diet was given to four groups of six chickens each. Growth performance and feed conversion ratio (FCR) were recorded weekly, from 7 to 21 d of age. In the second experiment, a digestion balance trial was performed from 27 to 31 d of age to evaluate the nitrogen-corrected apparent metabolizable energy (AMEn) and the digestibilities of nitrogen, crude fat, starch and crude fibre. From 7 to 21 d of age, xylanase supplementation led to increased final body weight and daily weight gain, by 3.7 and 4.5 % (P < 0.05), respectively, without significant difference according to the xylanase origin. Xylanase supplementation significantly increased the AMEn (+2.6 %), and the digestibilities of crude fibre (+58.9 %) and nitrogen (+1.6 %). Increase in AMEn as well as in crude fat and starch digestibilities were significantly different according to the xylanase, but were not dependent on fungal or bacterial origin. In conclusion, the microbial origin of xylanases supplemented to wheat-based diets influenced neither the performance of broiler chickens nor the improvement in nutrient digestibilities [less ▲]

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See detailEfficiency of a Lactobacillus plantarum-xylanase combination on growth performances, microflora populations, and nutrient digestibilities of broilers infected with Salmonella Typhimurium
Vandeplas, Sabrina ULg; Dubois Dauphin, Robin ULg; Thiry, Christophe ULg et al

in Poultry Science (2009), 88(8), 1643-1654

Three experiments were performed to assess the ability of a Lactobacillus plantarum probiotic combined with a xylanase to reduce the effects of S. typhimurium infection in broiler chickens from 1- to 30 ... [more ▼]

Three experiments were performed to assess the ability of a Lactobacillus plantarum probiotic combined with a xylanase to reduce the effects of S. typhimurium infection in broiler chickens from 1- to 30- or 42-d-old. Chicks were challenged at 3-d-old with 108 or 105 cfu S. typhimurium/chick. Four diets were studied: a wheat-based diet (C+) supplemented with 0.1 g/kg xylanase (E), or 106 cfu/g or L. plantarum (P), or both (PE). Uninfected chicks fed the C diet were used as negative control (C-). Six or 8 chicks were housed per cage with 9 cages/treatment. Growth performance and feed conversion ratio (FCR) were recorded weekly. In experiment 1, bacterial enumeration in caeca was achieved using the fluorescent in situ hybridization technique. Salmonella enumeration was realized in excreta by microbiological cultures (Exp. 2 and 3). Nutrient digestibilities and AMEn were determined in experiment 3 from d 35 to d 39. Infection with S. typhimurium led to a significant decrease in the daily weight gain (DWG) by 23.6% to 32.8%, whereas FCR was increased by 1.0% to 19.7%. Chickens fed the PE diet showed significantly improved performance in comparison with C+ birds (DWG: +12.5% in Exp. 1; FCR: -2.1-8.6%), and in comparison with the P and E treatments (DWG: +6.3-8.3% in Exp. 1; FCR: -2.7-6.4%). In experiment 3, the FCR was significantly improved by 3% with the PE diet in comparison with C- chickens. The PE combination tended to restore a microflora similar to that of uninfected broilers, whereas the P and E diets had less of an effect on the profile of bacterial communities. At slaughter age, Salmonella contamination was reduced by 2.00 and 1.85 log cfu for the E and PE treatment, respectively. The PE diet significantly reduced the crude fat digestibility by 9.2%, in comparison with the C+ chickens. These results suggest that combination between L. plantarum and a xylanase as feed additive could be effective for reduction of detrimental effect following S. typhimurium infection of broilers. [less ▲]

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See detailThe role of the specific immune response on cecal colonization of slow-growing broiler chickens reared on litter contaminated by Campylobacter jejuni
Vandeplas, Sabrina ULg; Dubois Dauphin, Robin ULg; Beckers, Yves ULg et al

Conference (2009)

The aim of this experiment was to study the relation between the specific immune response and infection of slow-growing broiler chickens reared on straw litter contaminated by Campylobacter jejuni. The 11 ... [more ▼]

The aim of this experiment was to study the relation between the specific immune response and infection of slow-growing broiler chickens reared on straw litter contaminated by Campylobacter jejuni. The 11-weeks experiment was carried out in a 45 m2 broiler house with 500 chickens from 1 day of age, which had access to an open-air range. Litter contamination was performed before the setting up of the chicks by rearing campylobacter-positive cocks. These birds were also used to contaminate the open-air range during one week before the exit of the chickens, at 5 weeks of age. Blood samples were taken weekly on 20 chickens for anti-campylobacter IgG quantitative measurements on serum. Chickens were then euthanized and cecal contents and bile were collected for Campylobacter enumeration and anti-campylobacter IgA quantification, respectively. A high serum anti-campylobacter IgG titer of 110.13 was measured in one-day-old chicks. The IgG antibodies level significantly decreased to 5.60 at 28 days of age which coincided with the onset of Campylobacter infection in the flock. A significant reduction of the cecal colonization from 11 weeks of age was correlated with the increase in biliary IgA titers. These results suggest that the 2-3 week’s delay generally observed in poultry production before flock infection by Campylobacter may be, at least partly, explained by the protective effect of maternal antibodies. The increase of secretory immune response as bird age may help to limit Campylobacter population in the chicken gut. [less ▲]

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See detailContamination of poultry flocks by the human pathogen Campylobacter spp. and strategies to reduce its prevalence at the farm level.
Vandeplas, Sabrina ULg; Marcq, Christopher ULg; Dubois Dauphin, Robin et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2008), 12(3), 317-334

Enteric Campylobacter spp. bacteria are human pathogens that frequently contaminate poultry flocks. Consumption of products from poultry origin may then lead to acute bacterial enteritis called ... [more ▼]

Enteric Campylobacter spp. bacteria are human pathogens that frequently contaminate poultry flocks. Consumption of products from poultry origin may then lead to acute bacterial enteritis called campylobacteriosis of which prevalence is increasing for about ten years in Europe. This review summarizes Campylobacter epidemiological data, risk factors for contamination in poultry flocks and conceivable strategies to control this pathogen. [less ▲]

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See detailPrevalence and sources of Campylobacter spp. contamination in free-range broiler production in Belgium
Vandeplas, Sabrina ULg; Dubois Dauphin, Robin ULg; Beckers, Yves ULg et al

in World's Poultry Science Journal (2006), 62(supplément), 557

An one year epidemiological study was carried out between February 2005 and January 2006 in Belgium to assess the Campylobacter prevalence in free-range broiler production. Three successive broiler flocks ... [more ▼]

An one year epidemiological study was carried out between February 2005 and January 2006 in Belgium to assess the Campylobacter prevalence in free-range broiler production. Three successive broiler flocks on 6 belgian farms were investigated for the presence of Campylobacter ssp. during the rearing period. Each flock was visited four times, before and after the outdoor rearing period. During each visit, samples were taken in the broiler houses (litter, cecal droppings, water-lines, feed, entrance premises) as well as from the outer rearing environment (open-air range). Conventional microbiological methods combined with biochemical tests were used for the Campylobacter detection, species identification and isolation. Campylobacter prevalence was very high in free-range broiler production during the experimental period. C. jejuni is the main species isolated from all contaminated samples, while mixed C. jejuni/C. coli infections sometimes occured. Contamination of the broiler flocks was increased in summer/autumn, with a 100% flocks contamination, whereas only 4 (66.7%) and 3 (50%) of the flocks became Campylobacter positive in spring and winter respectively, at the end of the rearing period. Moreover, about 53.8% of contaminated flocks were infected with Campylobacter before chicks have access to the open-air range. In 69.2% of the Campylobacter-positive flocks, the open-air range soil belonged partly of fully to environmental samples found to be Campylobacter-positive before flock infection. The other potential sources of infection were delivery tray, entrance premises floor and water-lines. The access to an open-air range seems to be an important way of contamination of broilers because Campylobacter prevalence in the flocks increased after going out. [less ▲]

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See detailSelection of bacteria to decrease in vitro growth of Campylobacter jejuni and Campylobacter coli and characterization of their antagonistic activities
Marcq, Christopher ULg; Didderen, Isabelle; Dubois Dauphin, Robin ULg et al

in Proceedings of the Eleventh Conference on Food Microbiology (2006)

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See detailValorisation du blé fourrager dans l'alimentation du poulet de chair.
Beckers, Yves ULg; Piron, Fabien ULg; Wéry, Olivier et al

Conference given outside the academic context (2005)

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See detailSécurité alimentaire et traçabilité
Vandeplas, Sabrina ULg; Romnee, Jean-Michel; Boudry, Christelle ULg et al

in Animal husbandry : yesterday, today and tomorrow. What are the expectations , And what are the challenges ? (2005)

La décennie 90 a été marquée par plusieurs crises: l'ESB, la présence d'hormones et la crise de la dioxine. La communauté scientifique a réagi en recherchant une meilleure sécurité alimentaire et une ... [more ▼]

La décennie 90 a été marquée par plusieurs crises: l'ESB, la présence d'hormones et la crise de la dioxine. La communauté scientifique a réagi en recherchant une meilleure sécurité alimentaire et une traçabilité efficace. La contribution de plusieurs chercheurs de la Faculté des Sciences Agronomiques et du Centre Wallon de Recherches Agronomiques est présentée dans cet exposé. [less ▲]

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See detailDes enzymes exogènes pour valoriser davantage le froment chez les volailles et les porcs.
Beckers, Yves ULg; Piron, Fabien ULg; Wéry, Olvier et al

Scientific conference (2005)

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See detailUtilization of a probiotic-hemicellulases combination to prevent effects of Salmonella contamination of broilers
Dubois Dauphin, Robin ULg; Vandeplas, Sabrina ULg; Welling, Gjalt W. et al

in Proceedings of the Ninth Conference on Food Microbiology (2004, September 16)

The aim of this study was to determine the effectiveness of a combination probiotic - hemicellulases by its relative incidence on gastrointestinal (GI) tract microbiota, correlated to the animal ... [more ▼]

The aim of this study was to determine the effectiveness of a combination probiotic - hemicellulases by its relative incidence on gastrointestinal (GI) tract microbiota, correlated to the animal performances during the growing period of broilers infected or not with Salmonella typhimurium [less ▲]

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See detailUtilization of a probiotic-hemicellulases combination to prevent effects of Samonella contamination of broilers.
Dubois Dauphin, Robin ULg; Vandeplas, Sabrina ULg; Welling, Gjalt et al

in Proceeding of the 9th Conference on Food Microbiology, University of Gent (Belgium), September 16-17, 2004 (2004, September)

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See detailEffect of hemicellulase preparations on performance parameters, intestinal viscosity and apparent nutrient digestibility of male broilers fed a wheat-based diet
Vandeplas, Sabrina ULg; Beckers, Yves ULg; Thewis, André ULg

Poster (2003)

Three experiments were conducted to investigate the mode of action of 5 hemicellulases supplemented to wheat-based broiler diets. Performances parameters and digesta viscosity were measured during growth ... [more ▼]

Three experiments were conducted to investigate the mode of action of 5 hemicellulases supplemented to wheat-based broiler diets. Performances parameters and digesta viscosity were measured during growth period. Total collection of excreta was carried out for determination of AMEn content of the diets and of apparent crude fat, Kjeldahl-nitrogen and crude cellulose digestibilities. Addition of xylanase improve daily body gain by 1 to 6% and feed conversion ration by 1,5 to 4%, as the apparent nutrient digestibility and the content of AMEn in the diets supplemented with enzymes. Although viscosity values seemed not correled to improved performances, Grindazym G shows an increase in the digesta viscosity for all the period of measurement. It can be concluded that the beneficial action of xylanases is more due to the improved release of the nutrients entrapped by the NSP, following degradation of these structures, than to the generally accepted decrease in digesta viscosity. [less ▲]

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See detailEtude du mode d'action d'hémicellulases ajoutées à un aliment riche en froment en alimentation aviaire : effet sur les performances zootechniques, la digestibilité des nutriments et la microflore intestinale
Vandeplas, Sabrina ULg

Master of advanced studies dissertation (2002)

Three experiments were conducted to investigate the effect of hemicellulase preparations on the performance parameters and the digestive physilogy of male broilers fed wheat-based diet from 1-32 d old ... [more ▼]

Three experiments were conducted to investigate the effect of hemicellulase preparations on the performance parameters and the digestive physilogy of male broilers fed wheat-based diet from 1-32 d old. The 5 dietary treatments were composed of a basal diet containing 60% (w/w) wheat (control) and 4 diets corresponding of the basal diet supplemented with enzyme preparations. Each diet was offered ad libitum. Two experiments were conducted for performances and digestibility measurements with 120 birds (24 birds/diet) in battery brooders housed in a environmentaly controlled room. Total collection of excreta was carried out from day 28 to day 32 post-hatching, for determination of apparent crude fat, Kjeldahl-nitrogen and crude cellulose digestibilities and for AMEn content of the diets measurement. The third experiment was conducted in pens frome day 7 to day 21. For each experimental diet, 16 birds were killed in 4 days for viscosity measurements in digesta. Addition of xylanase improve daily body gain by 1 to 6% without affecting ingestion of dry matter. Feed conversion ration increased by 1,5 to 4% with enzyme supplementation. Total apparent digestibilities of nitrogen, crude cellulose, crude fat and the content of AMEn in the 4 diets supplemented with enzymes increased significantly by an average of 1,6%, 59%, 1,7% and 2,8% respectively. Viscosity values were very variable between birds and seemed not correled to performances. However, digesta viscosity tend to decrease with enzyme addition, except for an enzyme (Grindazym G) wich induced an increase in the digesta viscosity for all the period of measurement. It is concluded from these experiments that improved performance parameters of broilers were more correled to the improvement of nutrients digestibility than to intestinal viscosity diminution. Digestion would be mainly improved by degradation of endosperm cell wall NSP and the subsequent release of the nutrients entrapped by the NSP structures, which induce more accessibility of nutriments to digestive endogenous enzymes of the birds. [less ▲]

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