References of "Vandelaer, Marc"
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See detailThree-dimensional organization of active rRNA genes within the nucleolus.
Cheutin, Thierry; O'Donohue, Marie*-Francoise; Beorchia, Adrien et al

in Journal of Cell Science (2002), 115(Pt 16), 3297-307

In this work, we have localized transcribing rRNA genes at the ultrastructural level and described their three-dimensional organization within the nucleolus by electron tomography. Isolated nucleoli ... [more ▼]

In this work, we have localized transcribing rRNA genes at the ultrastructural level and described their three-dimensional organization within the nucleolus by electron tomography. Isolated nucleoli, which exhibit a reduced transcriptional rate, were used to determine the sites of initial BrUTP incorporation (i.e. rRNA synthesis by the transcriptional machinery). Using pulse-chase experiments with BrUTP and an elongation inhibitor, cordycepin, it was possible to precisely localize the initial sites of BrUTP incorporation. Our data show that BrUTP incorporation initially takes place in the fibrillar centers and that elongating rRNAs rapidly enter the surrounding dense fibrillar component. Furthermore, we investigated the spatial arrangement of RNA polymerase I molecules within the whole volume of the fibrillar centers. Electron tomography was performed on thick sections of cells that had been labeled with anti-RNA polymerase I antibodies prior to embedding. Detailed tomographic analyses revealed that RNA polymerase I molecules are mainly localized within discrete clusters. In each of them, RNA polymerase I molecules were grouped as several coils, 60 nm in diameter. Overall, these findings have allowed us to propose a model for the three-dimensional organization of transcribing rDNA genes within the nucleolus. [less ▲]

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See detailUltrastructural study of human resting T lymphocytes and Sertoli cells: morphometric and immunocytochemical analysis
Vandelaer, Marc; Thiry, Marc ULg; Goessens, Guy ULg

in Belgian Journal of Zoology (1993), 123

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See detailDistribution of nucleic acids in cell nuclei by molecular immunocytochemistry
Thiry, Marc ULg; Vandelaer, Marc; Goessens, Guy ULg

Poster (1993)

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