References of "Vandelaer, M"
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See detailThree-dimensional organization of RNA polymerase I within fibrillar centers: a study by electron tomography
Ploton, D; Cheutin, T; O'Donohue, M-F et al

Conference (2001)

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See detailSites of BrUTP incorporation in isolated nucleoli of Ehrlich tumor cells
Thiry, Marc ULg; Vandelaer, M; Cheutin, T et al

Conference (2001)

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See detailAgNOR proteins from morphologically intact isolated nucleoli.
Vandelaer, M.; Thiry, Marc ULg; Goessens, Guy ULg

in Life Sciences (1999), 64(22), 2039-47

AgNOR staining has been proposed as a useful tool for the diagnosis and prognosis of cancer. The AgNOR proteins, however, have not yet been clearly identified and characterized, possibly due to the ... [more ▼]

AgNOR staining has been proposed as a useful tool for the diagnosis and prognosis of cancer. The AgNOR proteins, however, have not yet been clearly identified and characterized, possibly due to the partial character of the results obtained when studying the proteins extracted from altered nucleoli isolated by "standard" methods. In the present study, we analysed, on western blots, the AgNOR staining profiles obtained with protein extracts from Ehrlich tumor cell nucleoli isolated by a recent procedure that preserves the nucleolar ultrastructure. In addition to the well-known C23 and B23 protein bands, we readily detected an extra band at approximately 125 Kda. By immunoblotting, we showed that this polypeptide may be related to the nucleolar phosphoprotein pp135 evidenced in rat-cell nucleoli. By immunoelectron microscopy, we detected this protein in the dense fibrillar component and fibrillar center of the nucleoli as well as the coiled bodies. The distribution coincides with the cytochemical AgNOR staining pattern obtained at the ultrastructural level. [less ▲]

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See detailThe phosphoprotein pp135 is an essential constituent of the fibrillar components of nucleoli and of coiled bodies.
Vandelaer, M.; Thiry, Marc ULg

in Histochemistry & Cell Biology (1998), 110(2), 169-77

We examined the distribution of the silver-stainable phosphoprotein, pp135, within Ehrlich tumor and HEp-2 cells by a postembedding Lowicryl immunogold labeling procedure. Identical labeling patterns were ... [more ▼]

We examined the distribution of the silver-stainable phosphoprotein, pp135, within Ehrlich tumor and HEp-2 cells by a postembedding Lowicryl immunogold labeling procedure. Identical labeling patterns were obtained in both cell types. During interphase, gold particles were found not only over the dense fibrillar component but were also evident over the fibrillar centers of nucleoli. By contrast, the granular component did not display any significant label. When rRNA synthesis was inhibited by actinomycin D, the same labeling was observed in segregated nucleoli; both fibrillar components were labeled. Aside from the nucleolar labeling, label was also consistently present in coiled bodies. During metaphase, label was visualized in silver-stainable material of the nucleolus organizing regions. It thus appears that, unlike the two major silver-stained proteins, nucleolin/C23 and B23, pp135 remains located in all major silver-stainable structures during the whole cell cycle. This finding strongly suggests that pp135 could be the component responsible for in situ silver staining. On the other hand, the maintenance of pp135 in the fibrillar centers throughout the cell cycle, like RNA polymerase I, upstream binding factor, and DNA topoisomerase I, suggests that pp135 could be a component involved in transcription of the rRNA genes. [less ▲]

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See detailAg NORs proteins: identification in isolated nucleoli of Ehrlich tumour cells
Vandelaer, M; Thiry, Marc ULg; Goessens, G

in European Journal of Histochemistry (1996), 40

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See detailIdentification of Ag-NOR proteins from isolated nucleoli of Ehrlich tumor cells
Vandelaer, M; Thiry, Marc ULg; Goessens, G

Poster (1996)

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See detailLocation of an Ag-NOR protein in Ehrlich tumor cells by immunoelectron microscopy
Thiry, Marc ULg; Vandelaer, M; Goessens, G

Poster (1996)

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See detailAg-NORs proteins from isolated nucleoli of Ehrlich tumour cells
Vandelaer, M; Thiry, Marc ULg; Goessens, G

Poster (1996)

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See detailIsolation of nucleoli from ELT cells: a quick new method that preserves morphological integrity and high transcriptional activity.
Vandelaer, M.; Thiry, Marc ULg; Goessens, Guy ULg

in Experimental Cell Research (1996), 228(1), 125-31

We have developed a quick new method for isolating nucleoli which, unlike the methods in current use, preserves the nucleolar ultrastructure. Until now, the isolation process has generally been assumed to ... [more ▼]

We have developed a quick new method for isolating nucleoli which, unlike the methods in current use, preserves the nucleolar ultrastructure. Until now, the isolation process has generally been assumed to empty one of the three major compartments of the nucleolus, the fibrillar center, of its content. We have used the AgNOR staining and in vitro transcription assay to test the degree of structural and functional preservation of the isolated nucleoli. Our results demonstrate the value of our procedure as a reliable tool for biochemical and ultrastructural studies on the nucleolus. Moreover, these proprieties prompt us to investigate the rRNA synthesis, using a nonisotopic approach, within morphologically intact isolated nucleoli. Thus, we show that newly synthesized rRNA transcripts are located not only in the dense fibrillar component, but also indubitably in the fibrillar center. [less ▲]

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See detailAgNOR proteins from morphologically intact isolated nucleoli
Vandelaer, M; Thiry, Marc ULg; Goessens, G

Poster (1995)

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See detailCytochemical and biochemical studies of AgNOR proteins from isolated nucleoli
Vandelaer, M; Thiry, Marc ULg; Goessens, G

Poster (1995)

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See detailImmunoelectron microscope detection of newly synthetized RNA in isolated nucleoli of Ehrlich tumor cells
Thiry, Marc ULg; Vandelaer, M; Goessens, G

Poster (1995)

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See detailUltrastructural study of interchromatin granules during the cell cycle
Thiry, Marc ULg; Vandelaer, M; Goessens, G

Poster (1995)

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See detailUltrastructural distribution of DNA within the ring-shaped nucleolus of human resting T lymphocytes.
Vandelaer, M.; Thiry, Marc ULg; Goessens, Guy ULg

in Experimental Cell Research (1993), 205(2), 430-2

The precise distribution of DNA within the resting human T lymphocyte nucleolus has been investigated, at the ultrastructural level, by cytochemical and molecular immunocytochemical techniques. The ... [more ▼]

The precise distribution of DNA within the resting human T lymphocyte nucleolus has been investigated, at the ultrastructural level, by cytochemical and molecular immunocytochemical techniques. The nucleolus is partially enveloped by a layer of condensed chromatin which, at several places, penetrates into the nucleolar body until in close contact with the fibrillar centers. Morphometric analysis reveals that 32% of the fibrillar center surface is essentially occupied by condensed chromatin. Using the in situ terminal deoxynucleotidyl transferase-immunogold procedure for detecting DNA, we further show that evident label is exclusively found over the condensed chromatin and over the fibrillar centers, whereas no significant label is detected over the dense fibrillar component of the nucleolus. [less ▲]

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