A green and bio-inspired process to afford durable anti-biofilm properties to stainless steel

in Biofouling (2012), 28(7), 719-728

A bio-inspired durable anti-biofilm coating was developed for industrial stainless steel (SS) surfaces. Two polymers inspired from the adhesive and cross-linking properties of mussels were designed and ... [more ▼]

A bio-inspired durable anti-biofilm coating was developed for industrial stainless steel (SS) surfaces. Two polymers inspired from the adhesive and cross-linking properties of mussels were designed and assembled from aqueous solutions onto SS surfaces to afford durable coatings. Trypsin, a commercially available broad spectrum serine protease, was grafted as the final active layer of the coating. Its proteolytic activity after long immersion periods was demonstrated against several substrata, viz. a synthetic molecule, N-a-benzoyl-DL-arginine-p-nitroanilide hydrochloride (BAPNA), a protein, FTC-casein, and Gram-positive biofilm forming bacterium Staphylococcus epidermidis. [less ▲]

Antibacterial polyelectrolyte micelles for coating stainless steel

in Langmuir (2012), 28(18), 7233-7241

In this study, we report on the original synthesis and characterization of novel antimicrobial coatings for stainless steel by alternating the deposition of aqueous solutions of positively charged ... [more ▼]

In this study, we report on the original synthesis and characterization of novel antimicrobial coatings for stainless steel by alternating the deposition of aqueous solutions of positively charged polyelectrolytes micelles doped with silver based nanoparticles with a polyanion. The micelles are formed by electrostatic interaction between two oppositely charged polymers, a polycation bearing 3,4-dihydroxyphenylalanine units (DOPA, a major component of natural adhesives) and a polyanion (poly(styrene sulfonate), PSS) without using any block copolymer. DOPA units are exploited for their well-known ability to anchor to stainless steel and to form and stabilize biocidal silver nanoparticles (Ag0). The chlorine counter-anion of the polycation forms and stabilizes biocidal silver chloride nanoparticles (AgCl). We demonstrate that two layers of micelles (alternated by PSS) doped by silver particles are enough to impart to the surface a strong antibacterial activity against Gram-negative E. coli. Moreover, micelles that are reservoirs of biocidal Ag+ can be easily reactivated after depletion. This novel water-based approach is convenient, simple and attractive for industrial applications. [less ▲]

Biomolecule-based antibacterial coating on a stainless steel surface: multilayer film build-up optimization and stability study.

in Biofouling (2012), 28(4), 395-404

The goal of this paper was to establish the durability profile of antibacterial multilayer thin films under storage and usage conditions. Thin films were built on stainless steel (SS) by means of a layer ... [more ▼]

The goal of this paper was to establish the durability profile of antibacterial multilayer thin films under storage and usage conditions. Thin films were built on stainless steel (SS) by means of a layer-by-layer process alternating a negatively charged polyelectrolyte, polyacrylic acid, with a cationic antibacterial peptide, nisin. SS coupons coated with the antibacterial film were challenged under environmental and usage conditions likely to be encountered in real-world applications. The change in antibacterial activity elicited by the challenge was used as an indicator of multilayer film resistance. Antibacterial SS samples could be stored for several weeks at 4°C in ambient air and antibacterial films were resistant to dipping and mild wiping in water and neutral detergent. The multilayer coating showed some weaknesses, however, that need to be addressed. [less ▲]

Clay and DOPA containing polyelectrolyte multilayer film for imparting anticorrosion properties to galvanized steel

in Langmuir (2012), 28(5), 2971-2978

A facile and green approach is developed to impart remarkable protection against corrosion to galvanized steel. A protecting multilayer film is formed by alternating the deposition of a polycation bearing ... [more ▼]

A facile and green approach is developed to impart remarkable protection against corrosion to galvanized steel. A protecting multilayer film is formed by alternating the deposition of a polycation bearing catechol groups, used as corrosion inhibitors, with clay that induces barrier properties. This coating does not affect the esthetical aspect of the surface and does not release any toxic molecules in the environment. [less ▲]

Stainless steel with robust anibacterial activiy based on a versatile and bio-inspired strategy

Conference (2011, September 01)

The synthesis of a poly(methacrylamide) bearing 3,4-dihydroxyphenylalanine inspired from the chemical composition of mussel adhesives will be presented. This homopolymer is designed to insure a multilayer ... [more ▼]

The synthesis of a poly(methacrylamide) bearing 3,4-dihydroxyphenylalanine inspired from the chemical composition of mussel adhesives will be presented. This homopolymer is designed to insure a multilayer film growth by covalent coupling during the layer-by-layer building with a homopolymer containing amino groups. The film cross-linking is initiated by adequately controlling both the redox state of the polymer and the pH of the solutions. This cross-linking is evidenced by solid-state 13C NMR with the occurrence of a typical signal of imine, traducing the Schiff base formation during film growth. The same coupling reaction is implemented for grafting an antibacterial peptide, Nisin, on a stainless steel substrate. Antibacterial activity against Bacillus subtilis is preserved even after long immersion time, as the result of the peptide covalent anchoring on the surface. All the processing steps, including the synthesis of the polymers and the peptide grafting, are performed in aqueous solutions under mild conditions. [less ▲]

A water-based antibacterial nanohybrid solution for coating stainless steel

Conference (2011, June 28)

Sustainable and bio-inspired chemistry for robust antibacterial activity of stainless steel

in Journal of Materials Chemistry (2011), 21(22), 7901-7904

We report on the original synthesis of a poly(methacrylamide) bearing (oxidized) 3,4-dihydroxyphenylalanine specially designed to (i) insure film growth by covalent coupling, (ii) covalently bind an ... [more ▼]

We report on the original synthesis of a poly(methacrylamide) bearing (oxidized) 3,4-dihydroxyphenylalanine specially designed to (i) insure film growth by covalent coupling, (ii) covalently bind an antibacterial peptide and (iii) contribute to the film cross-linking that is essential for the durability of the properties. [less ▲]

Multifunctional coatings

Patent (2011)

New polyelectrolyte copolymer, composite material, multilayer film and substrate carrying such polyelectrolyte copolymer, composite and multilayer film wherein the polyelectrolyte copolymer comprises a) a ... [more ▼]

New polyelectrolyte copolymer, composite material, multilayer film and substrate carrying such polyelectrolyte copolymer, composite and multilayer film wherein the polyelectrolyte copolymer comprises a) a first type of identical or different units (A) each comprising one or more dihydroxyphenyl groups such that sidechains are present along the backbone of the polyelectrolyte copolymer which contain at least one dihydroxyphenyl group each; and (b1) a second type of identical or different units (B1) each comprising a cationic moiety, or (b2) a second type of identical or different units (B2) each comprising an anionic moiety. [less ▲]

Carbon Nanotubes Binding Peptides: Selection by Genetic Engineering, Binding Affinity Study

Poster (2011, January 31)

Biomimetic coatings with robust antibacterial properties

Conference (2010, July 01)