Distribution of different collagen types and fibronectin in neurofibromatosis tumours.
; ; et al
in Acta Pathologica, Microbiologica, et Immunologica Scandinavica. Section A-Pathology (1984), 92(5), 345-52
Collagen types I, III, IV and V and fibronectin were localized in neurofibromas from six patients with von Recklinghausen's neurofibromatosis (NF) using indirect immunofluorescence (IIF) and peroxidase ... [more ▼]
Collagen types I, III, IV and V and fibronectin were localized in neurofibromas from six patients with von Recklinghausen's neurofibromatosis (NF) using indirect immunofluorescence (IIF) and peroxidase anti-peroxidase (PAP) techniques. Type I and III collagens were abundantly and rather evenly present in the tumours and formed a continuous network, but were absent from the capillary endothelial walls and were sparse in the perineurium of the occasional nerve fascicles. The type III/type I + type III collagen ratio in neurofibromas varied from 17.4% to 37.3% when estimated with cyanogen bromide peptide analysis. Fibronectin was detected in areas where type I and III collagens were present but was most intensively stained in the vascular walls and perineurium. Type IV collagen was detected at the dermo-epidermal junction of the skin overlying the tumours, in the endothelial cells of the capillaries, the perineurium and endoneurium. Furthermore, in the tumourous stroma there was plenty of type IV collagen appearing as a discontinuous patchy pattern suggesting abundant basement membrane material associated with cells forming the tumours. Type V collagen distribution was very similar to that of type IV collagen. [less ▲]Detailed reference viewed: 4 (0 ULg)
Morphogenesis and fibronectin in sexual differentiation of rat embryonic gonads.
; ; et al
in Differentiation : Research in Biological Diversity (1983), 23(Suppl), 72-81
The possible role of fibronectin in the organization of the sex-specific gonadal components was studied by immunocytochemistry combined with electron and light microscopy in rat fetuses at the ages of 12 ... [more ▼]
The possible role of fibronectin in the organization of the sex-specific gonadal components was studied by immunocytochemistry combined with electron and light microscopy in rat fetuses at the ages of 12-15 days. Fibronectin was evenly distributed in both sexes under the basal lamina of the surface epithelium. Other basal laminae were not seen using light or electron microscopy inside the gonadal ridges at the age of 12 days. As the first sign of sexual differentiation, fibronectin-negative gonadal cords appeared in 13-day-old fetuses. In the males the cords were bigger than those in the females. The cords were clearly separated from the interstitium in 15-day-old fetuses of both sexes. A continuous layer of fibronectin had formed between the testicular surface epithelium and the elongated cords indicating the formation of a tunica albuginea. In females the surface epithelium-cord connection was maintained at all stages. Connections of the gonadal cords to mesonephric tubuli were seen in the rete region of both sexes. The electron optical basal lamina around the gonadal cords became continuous by the age of 15 days. The present results suggest that fibronectin is intimately involved in the sexual differentiation of the gonads, but not under the regulation of H-Y antigen or other testis-organizing factor. [less ▲]Detailed reference viewed: 6 (0 ULg)
Changes in the distribution of type IV collagen, laminin, proteoglycan, and fibronectin during mouse tooth development
; ; Foidart, Jean-Michel et al
in Developmental Biology (1981), 81(1), 182-92Detailed reference viewed: 1 (0 ULg)