References of "Tine, Emmanuel"
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See detailPhysicochemical characterization of dextrins prepared with amylases from sorghum malt
Ba, Khady; Blecker, Christophe ULg; Danthine, Sabine ULg et al

in Stärke = Starch (2013)

Dextrins are partially hydrolyzed starch products which are used in a wide range of applications. Hydrolysis can be performed by acid or enzymatic reaction. However the properties of dextrins are ... [more ▼]

Dextrins are partially hydrolyzed starch products which are used in a wide range of applications. Hydrolysis can be performed by acid or enzymatic reaction. However the properties of dextrins are influenced by the type of reaction and the source of starch. The aim of the present study was to determine physicochemical properties of dextrins obtained from hydrolysis of starches (corn and wheat) and flours (wheat and cassava) by using sorghum malt amylases. Hydrolysis of starches and flours was performed at 65°C, the hydrolysates were centrifuged and spray-dried for analysis. Physicochemical and structure of the powders were investigated. Results showed that the proportion of DP2 (maltose and isomaltose) was higher in dextrins and more in dextrins from wheat flour. Low water activity and low dissolution time were found in all dextrins. XRD patterns revealed that the crystalline structure partially disappeared in some of them. At 30% concentration, we noted a Newtonian behavior for the dextrins. The freeze-concentrated T0 g and the peak melting temperature (T0 m) determined by DSC (DSC) showed lowest values for dextrins from wheat. Thermogravimetric analysis (TGA) revealed that the dextrins were quite stable until 200°C. [less ▲]

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See detailHydrolysis of starches and flours by sorghum malt amylases for dextrins production
Ba, Khady ULg; Aguedo, Mario ULg; Tine, Emmanuel et al

in European Food Research & Technology (2013)

Corn and wheat starches as well as wheat and cassava flours were hydrolyzed using sorghum malt at 65 °C for 6 hours. During these reactions, dextrose equivalent (DE) values were followed under 3 ... [more ▼]

Corn and wheat starches as well as wheat and cassava flours were hydrolyzed using sorghum malt at 65 °C for 6 hours. During these reactions, dextrose equivalent (DE) values were followed under 3 concentrations of sorghum malt and of calcium chloride. Wheat flour presented the highest DE values and cassava flour had the highest hydrolysis yield. Thus, different dextrins were produced in a pilot plant and were analyzed by HPSEC and HPAEC-PAD for their molecular weight distribution and oligosaccharides composition respectively. The results indicated that oligosaccharides with broad molecular weight distributions were present in the dextrins produced and that the proportion of maltose was very high. [less ▲]

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See detailEtude comparative des composés phénoliques, du pouvoir antioxydant de différentes variétés de sorgho sénégalais et des enzymes amylolytiques de leur malt
Ba, Khady ULg; Tine, Emmanuel; Destain, Jacqueline ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2010), 14(1), 131-139

The study involved seven cultivars of white sorghum selected to ISRA Bambey and consumed in many regions of Senegal. Several characters and biochemical compounds of the grains (presence of pigmented testa ... [more ▼]

The study involved seven cultivars of white sorghum selected to ISRA Bambey and consumed in many regions of Senegal. Several characters and biochemical compounds of the grains (presence of pigmented testa, total phenols, condensed tannins and antioxidant activity) were determined in all cultivars. A three days malting was also carried out and amylolytic enzymes such as α-amylase, β-amylase and limit-dextrinase which are essential for a malt of good quality were proportioned. These enzymes are measured specifically with kits of Megazyme: Amylazyme (α-amylase), Betamyl (β-amylase) and Limit-Dextrizyme (limit-dextrinase). Two cultivars, CE 180-33 and CE 145-66 proved to be tannin sorghums, have the highest levels in total phenols and the most important antioxidant activities (ABTS and DPPH). The results of enzymatic analysis and the index of Kolbach indicated the F-2-20 like cultivar presenting the best potentialities for malt production [less ▲]

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See detailLa conservation du poisson au Senegal : utilisation d’une souche locale de Lactococcus lactis
Diop, Michel; Destain, Jacqueline ULg; Thonart, Philippe ULg et al

in Cahiers Agricultures (2009), 18(4), 337-342

L’effet antimicrobien du surnageant de culture neutralisé (SCN) bactéricide issu de Lactococcus lactis CWBI-B1410 d’origine locale, utilisé seul ou en combinaison avec du chlorure de sodium (NaCl) comme ... [more ▼]

L’effet antimicrobien du surnageant de culture neutralisé (SCN) bactéricide issu de Lactococcus lactis CWBI-B1410 d’origine locale, utilisé seul ou en combinaison avec du chlorure de sodium (NaCl) comme conservateurs, a été évalué sur poissons maigre (Pomadasys jubelini), moyennement gras (Polydactylus quadrifilis) et gras (Arius heudeloti)] au Sénégal. Les poissons ont été achetés dans un marché local, éviscérés, lavés avec de l’eau de robinet potable, puis filetés. Cent millilitres de SCN de CWBI-B1410 non salé et salé ont été additionnés dans 100 grammes de filets (concentration finale de NaCl entre 0 et 7 %) dans des bocaux en verre conservés à 10 °C. L’évolution de la flore mésophile totale (FMT) des filets a été comparée à celles de filets traités avec du SCN non salé et salé (concentration finale de NaCl entre 0 et 7 %), issu de L. lactis LMG6890 ne produisant pas de bactériocine. Un niveau de FMT de 106 micro-organismes par gramme (ufc/g) a été considéré comme la fin de la durée de conservation. Le niveau de la flore mésophile totale des filets crus atteignait 5,74 log ufc/g. L’ajout de SCN de CWBI-B1410 dans les filets de poisson maigre réduit la flore mésophile totale de 1 log ufc/g et stabilise la flore pendant 4 jours – correspondant à la durée de conservation de ces filets a` 10 °C, contre 0,5 jour pour le contrôle négatif. L’addition de SCN de CWBIB1410 salé sur les poissons (NaCl, 7 %) réduit davantage la FMT et retarde sa croissance à 10°C, entraînant comme résultat l’augmentation de la durée de conservation de respectivement 12, 7,5 et 8 jours pour le poisson maigre, moyennement gras et gras. Ces résultats suggèrent que cette stratégie peut constituer un moyen convenable d’améliorer la conservation des produits halieutiques tropicaux. [less ▲]

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See detailUse of a Nisin-Producing Starter Culture of Lactococcus lactis subsp. lactis To Improve Traditional Fish Fermentation in Senegal
Diop, Michel; Dubois Dauphin, Robin ULg; Destain, Jacqueline ULg et al

in Journal of Food Protection (2009), 72(9), 1930-1934

Lactococcus lactis subsp. lactis strain CWBI B1410, which produces various antibacterial compounds including organic acids and nisin, was used as a starter culture to improve the traditional Senegalese ... [more ▼]

Lactococcus lactis subsp. lactis strain CWBI B1410, which produces various antibacterial compounds including organic acids and nisin, was used as a starter culture to improve the traditional Senegalese fish fermentation in which fish are mostly transformed to guedj by spontaneous fermentation for 24 to 48 h at ambient temperatures near 30uC followed by salting (with NaCl) and sun drying. Assays were performed on lean fish (Podamasys jubelini) and fat fish (Arius heudelotii) purchased at a local market. The total viable microbial counts in raw fillets of P. jubelini and A. heudelotii were 5.78 and 5.39 log CFU/g, respectively. Populations of enteric bacteria (which can include pathogenic bacteria) in P. jubelini and A. heudelotii were 4.08 and 4.12 log CFU/g, respectively. Spontaneous fermentation of raw fillets at 30uC led to the proliferation of enteric bacteria to 9 log CFU/g after 24 h in fermented P. jubelini and A. heudelotii fillets with pH values of 6.83 and 7.50, respectively. When raw fish fillets were supplemented with glucose (1%, wt/wt) and inoculated with Lactococcus lactis (107 CFU/g), the pH decreased to about 4.60 after 10 h at 30uC, and nisin activity was detected in juice from the fillets. Traditionally fermented fillets of P. jubelini and A. heudelotii contained enteric bacteria at higher levels of 4 and 2 log CFU/g, respectively, than did fillets of the same fish supplemented with glucose and fermented with the starter culture. These data suggest that this new fish fermentation strategy combined with salting and drying can be used to enhance the safety of guedj [less ▲]

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See detailBacteriocin producers from traditional food products
Diop, Michel Bakar; Dubois Dauphin, Robin ULg; Tine, Emmanuel et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2007), 11(4), 275-281

A total of 220 strains of LAB isolated from 32 samples of traditional fermented food from Senegal were screened for bacteriocin production. Two bacteriocin producers, Lactococcus lactis subsp. lactis and ... [more ▼]

A total of 220 strains of LAB isolated from 32 samples of traditional fermented food from Senegal were screened for bacteriocin production. Two bacteriocin producers, Lactococcus lactis subsp. lactis and Enterococcus faecium, were identifi ed from 12 bacteriocin-producing isolates on the basis of phenotypic analyses and 16S rDNA sequence. Both bacteriocins produced by new isolates show antimicrobial activity against Listeria monocytogenes and Bacillus coagulans whereas only that produced by Lactococcus lactis has an activity against Bacillus cereus. Bacteriocin-producing Lactococcus lactis strains were found in a variety of traditional foods indicating a high potential of growth of this strain in variable ecological complex environment. Partial 16S rDNA of the two bacteriocin producers obtained in this study has been registered to Genbank databases under the accession number AY971748 for Lactococcus lactis subsp. lactis (named CWBI-B1410) and AY971749 for Enterococcus faecium (named CWBI-B1411). The new bacteriocin-producing Lactococcus lactis subsp. lactis strain has been selected for identification and application of the bacteriocin to food preservation. [less ▲]

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