References of "Thomé, Jean-Pierre"
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See detailDistribution of inorganic and organic contaminants in the intertidal surface sediments along the Hugli River Estuary, eastern part of India
Das, Krishna ULg; Sarkar, Santosh; Thomé, Jean-Pierre ULg et al

Conference (2016, February 17)

Hooghly estuary and coastal environment in north east coast of India has been strongly affected by the rapid human settlements, intensive boating and tourist activities, deforestation and ongoing ... [more ▼]

Hooghly estuary and coastal environment in north east coast of India has been strongly affected by the rapid human settlements, intensive boating and tourist activities, deforestation and ongoing agricultural and aquacultural practices. The present work investigated trace elements (TEs, T-Hg, Al, V, Cr, Fe, Mn, Cu, Zn, Co, Ni, Ca, Sr, Li, Be, P, Mo, Ag, Cd, Sn, Sb, Ba, Pb, Bi, U, As, Se) and organohalogen compounds in surface sediment (top 0-10cm) considering 8 sampling sites along the Hugli River Estuary (HRE), India. The studied organic compounds include polychlorobiphenyls (PCBs), hexachlorocyclohexane isomers (HCHs), dichlorodiphenyltrichloroethane and metabolites (DDTs) and polybromodiphenyl ethers (PBDEs). Textural analyses of the sediment reveal an overall dominance of mud. All trace elements could be detected at variable concentrations in the following descending order: Al>Fe>Ca> P>Mn> Cu> Zn> Ba >V >Cr>Sr>Ni> Li >Co >Pb>As >Se >U >Be >Sn>Bi >Ag >Mo >Cd >Sb. A synchronous enrichment of majority of the elements were observed at the site Gangasagar (~100 km south megacity Calcutta), situated at the confluence of Hugli River and Bay of Bengal, which might be attributed to cumulative impact of the physicochemical processes and multiple anthropogenic sources. It is revealed that PCBs and DDTs were the dominant compounds among the organic pollutants accounting concentrations up to 23.5 ng.g-1 and 4.4 ng.g-1 respectively at the site Babughat, adjacent to the megacity Calcutta. The results have implications for pollution in complex estuarine environment in a meso-macrotidal setup where there is significant influx of sediments carrying huge load of inorganic and organic pollutants mainly due to rapid urbanization and industrialization. [less ▲]

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See detailMercury and persistent organic pollutant concentrations in free-ranging bottlenose dolphins from Lower Keys and Coastal Everglades (South Florida)
Damseaux, France ULg; Kiszka, Jeremy; Heithaus, Michael et al

Poster (2016, February 12)

The bottlenose dolphin (Tursiops truncatus) is a major apex predator and the most common cetacean species found in nearshore waters of South Florida, including the Lower Florida Keys (LFK) and the Florida ... [more ▼]

The bottlenose dolphin (Tursiops truncatus) is a major apex predator and the most common cetacean species found in nearshore waters of South Florida, including the Lower Florida Keys (LFK) and the Florida Coastal Everglades (FCE). The objective of this study was 1) to assess contamination levels of total mercury (T-Hg) in skin and persistent organic pollutants (PCBs, PBDEs, DDT, HCH, HCB, DLCs and PCDD/Fs) in blubber samples of bottlenose dolphins from the LFK (8 males and 16 females) and from the FCE (13 males and 9 females). Pollutants were analysed by the mean of Direct Mercury analyser (for T-Hg), GC-ECD (POPs) and GC-MS (DLCs and PCDD/Fs). The PCBs were the main compounds found in bottlenose dolphins from the LFK and FCE. The most present congeners where the CB no. 28, 52, 101, 138, 153 and 180 (ΣPCBs LFK males: 13420.5 ng.g-1 lipids, ΣPCBs LFK females: 9683.4 ng.g-1 lipids, ΣPCBs FCE males: 5637.9 ng.g-1 lipids, ΣPCBs FCE females: 1426.9 ng.g-1 lipids). PCBs concentrations were significantly higher in individuals from the LFK than those from the FCE but significantly lower than those from other locations in the south-eastern US. Unlike organic pollutants, T-Hg concentrations were significantly higher in FCE male dolphins (LFK: 2936.0 ng.g-1 dw, FCE: 10048.3 ng.g-1 dw). These high concentrations were the highest recorded in the south-eastern US and are most likely due to the presence of mangrove ecosystems. This study highlights the complexity of contaminant dynamics (inorganic vs. organic), even at small spatial scales. [less ▲]

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See detailImpact of three phtalate esters on the sexual reproduction of the Monogont rotifer, Brachionus calyciflorus
Cruciani, Valentina ULg; Iovine, Catherine; Thomé, Jean-Pierre ULg et al

in Ecotoxicology (2016), 25(1), 192-200

Phthalate esters are widespread contaminants that can cause endocrine disruption in vertebrates. Studies showed that molecules with hormonal activities in vertebrates and invertebrates can affect asexual ... [more ▼]

Phthalate esters are widespread contaminants that can cause endocrine disruption in vertebrates. Studies showed that molecules with hormonal activities in vertebrates and invertebrates can affect asexual and sexual reproduction in rotifers. We investigated the impact of di-hexylethyl phthalate (DEHP), di-butyl phthalate (DBP) and butylbenzyl phthalate (BBP), on the asexual and sexual reproduction of the freshwater monogonont rotifer Brachionus calyciflorus in order to determine a potential environmental risk for sexual reproduction. We observed that DEHP has no significant impact on both asexual and sexual reproduction up to 2 mg/L. DBP has a positive effect on asexual reproduction at concentrations from 0.05 to 1mg/L, but depresses it at 2 mg/L. Sexual reproduction is only affected with 2mg/L and the impact observed is negative. BBP displayed a negative impact on both asexual and sexual reproduction at 1 and 2 mg/L. However we showed that the impacts of BBP on fertilization rates observed are due to the decrease in population growth rates at these concentrations and are not due to a direct impact of BBP on the fertilization process. Similar conclusions are likely applicable to the impacts on mixis and production of cysts as well. Our results show that sexual reproduction in B. calyciflorus is not more sensitive than asexual reproduction to any of the substances tested which indicates the mode of action of these molecules is related to general toxicity, not to an interference with potential endocrine regulation of sexual reproduction. Comparison of effect concentrations and surface water contamination by phthalate esters suggests these compounds do not constitute a risk for these environments. [less ▲]

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See detailVitellogenin and vitellogenin receptor gene expression and 20-hydroxyecdysone concentration in Macrobrachium rosenbergii exposed to chlordecone
Lafontaine, Anne ULg; Hanikenne, Marc ULg; Boulangé-Lecomte, Céline et al

in Environmental Science and Pollution Research (2016)

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See detailTranscriptome of the freshwater amphipod Gammarus pulex hepatopancreas
Gismondi, Eric ULg; Thomé, Jean-Pierre ULg

in Genomics Data (2016), 8

So far, ecotoxicological studies used biomarkers of exposure or of effects in order to investigate the impacts of contaminated areas on biota (Peakall, 1994 [6]). However, although these results are ... [more ▼]

So far, ecotoxicological studies used biomarkers of exposure or of effects in order to investigate the impacts of contaminated areas on biota (Peakall, 1994 [6]). However, although these results are important in the ecotoxicological risk assessment, biomarkers are very specific and only provide information on the biological processes or physiological pathways targeted by the biomarkers experimenters choose to test (Monsinjon and Knigge, 2007 [5]). In recent years, proteomics have become a major tool in ecotoxicology, as they provide a global insight into the mechanism of action of pollutants without the need of hypothesis testing or any preconception on the biological processes likely impacted (Gismondi et al., 2015; Trapp et al., 2015 [7]; Truebano, 2016 [8]). However, the analysis of proteomic results is often limited due to the lack of database, especially for non-model organisms, such as Gammarus sp, commonly used as biological model in ecotoxicology (Sornom et al., 2012 [11]; Vellinger et al., 2013 [9]; Gismondi and Thomé, 2014 [1]; Lebrun et al., 2014 [3]). Here, we performed Illumina HiSeq sequencing to total RNA isolated from the hepatopancreas (i.e. detoxification tissue) of Gammarus pulex males and females coming from uncontaminated river and contaminated river (e.g. PCB, benzo(a)pyrene). Approximately 290Mpaired-end readswere assembled, filtered and sorted into 39,801 contigswhose 10.878 were similar of proteins available in databases. The assembled contigs could represent a reference hepatopancreas transcriptome for G. pulex, and constitute an important resource for future investigations on the impacts of pollutants on invertebrate biota, since itwould improve the understanding of the mechanisms of action involved in toxicity. In addition, the hepatopancreas transcriptome will also allow the identification of new potential biomarkers for the ecotoxicological risk assessments. Assembled contigswere deposited in the European Nucleotide Archive under the BioProject number PRJEB13055, with accession numbers FJVI01000001-FJVI01039801. [less ▲]

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See detailSpatial variations in the concentrations of mercury and persistent organic pollutants in free-ranging bottlenose dolphins from South Florida
Das, Krishna ULg; Damseaux, France ULg; Heithaus, M.R. et al

Poster (2015, December)

The bottlenose dolphin (Tursiops truncatus) is an abundant top predator found in nearshore waters of South Florida including heavily developed and remote coastal waters. The objective of this study was to ... [more ▼]

The bottlenose dolphin (Tursiops truncatus) is an abundant top predator found in nearshore waters of South Florida including heavily developed and remote coastal waters. The objective of this study was to quantify total mercury (T-Hg) and persistent organic pollutant (POPs: NDL-PCBs, PBDEs, DDT, HCH, HCB, PCDD/Fs and DL-PCBs) levels in bottlenose dolphins found off the highly populated island of Key West (n = 27) and from the undeveloped Florida Coastal Everglades (FCE; n = 20). T-Hg and POPs were analyzed in skin and blubber tissues, respectively, using a Direct Mercury analyzer (for T-Hg), GC-ECD (POPs) and GC-HRMS (DLCs). The 7 ICES PCBs were the main compounds found in bottlenose dolphins from Key West (8229 ng.g-1 lipids) and the FCE (2289 ng.g-1 lipids), while the concentrations of PCDD/Fs remained low (Key West: 104 pg.g-1 lipids, FCE: 102 pg.g-1 lipids). POP concentrations were higher in individuals from Key West compared to those from the FCE. However, POP concentrations in Key West dolphins were lower than those from other locations in Florida and around the world. Unlike organic pollutants, T-Hg concentrations were significantly higher in FCE dolphins (Key West: 2941 ng.g-1 dw versus, FCE: 9314 ng.g-1 dw), with the highest concentrations reported from the southeastern US. PCB concentrations remained under previously suggested threshold for adverse health effects (including immunosuppression) in marine mammals of 17 000 ng/kg lipid. To conclude, sources of T-Hg and POPs differed between Key West and the FCE as reflected by their concentrations in skin and blubber of free-ranging bottlenose dolphins highlighting their role as sentinels of their environment. [less ▲]

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See detailGender differences in responses in Gammarus pulex exposed to BDE-47: a gel-free proteomic approach
Gismondi, Eric ULg; Mazzucchelli, Gabriel ULg; De Pauw, Edwin ULg et al

in Ecotoxicology & Environmental Safety (2015), 122

Very few ecotoxicological studies have considered differences in toxic effects on male and female organisms. Here, we investigated protein expression differences in caeca of Gammarus pulex males and ... [more ▼]

Very few ecotoxicological studies have considered differences in toxic effects on male and female organisms. Here, we investigated protein expression differences in caeca of Gammarus pulex males and females under control conditions (unexposed) and after 96 h exposure to BDE-47. Using gel-free proteomic analysis, we have identified 45 proteins, of which 25 were significantly differently expressed according to sex and/or BDE-47 exposure. These proteins were involved in several biological processes such as energy metabolism, chaperone proteins, or transcription/translation. In unexposed amphipods, 11 proteins were significantly over-expressed in females, and 6 proteins were over-expressed in males. Under BDE-47 stress, 7 proteins were differently impacted according to sex. For example, catalase was over-expressed in exposed females and under-expressed in exposed males, as compared to respective controls. Conversely, proteins involved in energy metabolism were up-regulated in males and down-regulated in females. Our proteomic study showed differences in responses of males and females to BDE-47 exposure, emphasizing that sex is a confounding factor in ecotoxicological assessment. However, due to the limited information existing in databases on Gammarids, it was difficult to define a BDE-47 mechanism of action. The gel-free proteomic seems to be a promising method to develop in future ecotoxicological studies and thus, to improve our understanding of the mechanism of action of xenobiotics. [less ▲]

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See detailSTAtion of Reference and rEsearch on Change of local and global Anthropogenic Pressures on Mediterranean Ecosystems Drifts: The STARECAPMED project
Richir, Jonathan ULg; Abadie, Arnaud ULg; Binard, Marc ULg et al

Conference (2015, November 08)

The Marine and Oceanographic Research Station STARESO in the Calvi Bay, Corsica (France), is a unique tool in a preserved natural site that includes all the characteristic ecosystems of the Mediterranean ... [more ▼]

The Marine and Oceanographic Research Station STARESO in the Calvi Bay, Corsica (France), is a unique tool in a preserved natural site that includes all the characteristic ecosystems of the Mediterranean littoral. The station, established in 1970, has archived environmental data for decades. The STARECAPMED project, multidisciplinary, articulates itself around these two main features. Its objective is to understand how human activities can interact with the fundamental processes that govern the functioning of the different coastal ecosystems of a Mediterranean bay. The understanding of these interactions involves: (i) the identification of the anthropogenic pressures; (ii) the quantification of their impacts on the ecosystems; (iii) the prioritization of these impacts. STARECAPMED also aims to confirm the relevance of the use of the Calvi Bay as a reference in the study of local and global pressures and the changes they may cause on the structure and the functioning of Mediterranean coastal ecosytems. [less ▲]

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See detailSpatial variations in concentrations of mercury and persistent organic pollutants in coastal bottlenose dolphins, Tursiops truncatus, from the Lower Florida Keys and the coastal Everglades (South Florida)
Damseaux, France ULg; Kiszka, Jeremy; Heithaus, Michael et al

Conference (2015, October 09)

The bottlenose dolphin (Tursiops truncatus) is a major apex predator and the most common cetacean species found in nearshore waters of South Florida, including the Lower Florida Keys (LFK) and the ... [more ▼]

The bottlenose dolphin (Tursiops truncatus) is a major apex predator and the most common cetacean species found in nearshore waters of South Florida, including the Lower Florida Keys (LFK) and the Everglades National Park (ENP). The objective of this study was 1) to assess contamination levels of total mercury (T-Hg) in skin and persistent organic pollutants (PCBs, PBDEs, DDT, HCH, HCB, DLCs and PCDD/Fs) in blubber samples of bottlenose dolphins from the LFK (T-Hg : nmales = 10; POPs : nmales = 16, nfemales = 8) and the ENP (T-Hg : nmales = 13, nfemales = 9; POPs : nmales = 11, nfemales = 8). T-Hg and POPs were analysed by the mean of Direct Mercury analyser (for T-Hg), GC-ECD (POPs) and GC-MS (DLCs and PCDD/Fs). The PCBs were the main compounds found in bottlenose dolphins from the LFK and ENP. The most present congeners where the CB no. 28, 52, 101, 138, 153 and 180 (Σ 6 PCBs LFK males: 13420.5 ng.g-1 lipids, Σ 6 PCBs LFK females: 9683.4 ng.g-1 lipids, Σ 6 PCBs ENP males: 5637.9 ng.g-1 lipids, Σ 6 PCBs ENP females: 1426.9 ng.g-1 lipids), while the concentrations of DL PCBs remained low for both locations (LFK: 739 ng.g-1 lipids, ENP: 183 ng.g-1 lipids). PCBs concentrations were significantly higher in individuals from the LFK than those from the ENP. PCBs concentrations in LFK and ENP dolphins were significantly lower than those from other locations in the south-eastern US. Unlike organic pollutants, T-Hg concentrations were significantly higher in ENP male dolphins (LFK: 2936.0 ng.g-1 dw, ENP: 10048.3 ng.g-1 dw). These high concentrations were the highest recorded in the south-eastern US and are most likely due to the presence of mangrove ecosystems. This study highlights the complexity of contaminant dynamics (inorganic vs. organic), even at small spatial scales. [less ▲]

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See detailAssessment of Pollution in Sidi M'Hamed Benali Lake (Algeria) Based on Bioindicators and Physicochemical Parameters
El Badaoui, Naïma; Amar, Youcef; Joaquim-Justo, Célia ULg et al

in Iranica Journal of Energy & Environment (2015), 6(4), 308-315

This study was carried out to investigate the degree, the nature and the origin of pollution in Sidi M’hamed Benali Lake using the physicochemical parameters, saprobic index and cladocerans. For this ... [more ▼]

This study was carried out to investigate the degree, the nature and the origin of pollution in Sidi M’hamed Benali Lake using the physicochemical parameters, saprobic index and cladocerans. For this purpose, water and zooplankton sampling was collected from six sites in lake during five seasons. The average seasonal values of physicochemical parameters showed that the lake undergoes a slight anthropogenic and natural pollution in the dry and wet periods. Presence of certain toxic substances (CN-, Cr, Ni) require us to be more careful in irrigation, bathe and the consumption of fishes of that reservoir. Overall, oligo-mesosaprobic to beta-mesosaprobic rotifers have been prevailing in all five seasons indicating that the water was slightly or moderate polluted. The presence of Bosmina longirostris, Daphnia longispina, Daphnia cuculata, Daphnia ambiga and Sididae diaphonosoma brachyrum indicate bacterial contamination with the intense development of the phytoplankton in the lake, especially in springs and summer. Pearson correlation analysis revealed significant correlation between all of the physicochemical parameters. However, it revealed no significant correlation between zooplanktons occurrence and the majority environmental variables values. In present investigation, the Lake water is relatively little exposed to pollution and does not undergo strong organic pollution. [less ▲]

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See detailSTARECAPMED (STAtion of Reference and rEsearch on Change of local and global Anthropogenic Pressures on Mediterranean Ecosystems Drifts) - Année 2014. Rapport de recherches.
Richir, Jonathan ULg; Abadie, Arnaud ULg; Binard, Marc ULg et al

Report (2015)

La prise de conscience, par le grand public, de l'impact grandissant de l'homme sur l'océan est récente. Elle se traduit par une volonté politique sincère de correction par des mesures de protection, de ... [more ▼]

La prise de conscience, par le grand public, de l'impact grandissant de l'homme sur l'océan est récente. Elle se traduit par une volonté politique sincère de correction par des mesures de protection, de gestion et de développement durable. Ces politiques, et leurs conséquences économiques et sociétales lourdes, ne peuvent être acceptées que si les décisions se fondent sur des connaissances scientifiques incontestables et montrent des résultats scientifiquement prouvés. Par ailleurs, ces décisions doivent prendre en compte des impacts qui s'opèrent à des échelles de temps et d’espace très variables, de quelques heures à plusieurs dizaines d’années et de quelques mètres à plusieurs milliers de km. En termes politiques, l'information scientifique nécessaire à la prise de décision doit pouvoir couvrir les différentes échelles depuis le niveau local et régional, jusqu'à l'échelle nationale, européenne voire globale, et cela sur le plus long terme possible. Enfin, pour être complète, l'information scientifique sur les écosystèmes marins doit pouvoir répondre à trois questions objectives : (i) quel est l'état? (ii) quelle est l'évolution? (iii) quels sont les mécanismes et processus mis en jeux? et à une question plus prospective : (vi) que peux-t-on prévoir et comment agir? Le présent rapport ne peut pas, à lui seul, refléter toute la richesse du programme STARECAPMED. En 3 ans, STARECAPMED a généré plusieurs centaines de milliers de données nouvelles, ré-exploité plusieurs centaines de publications, rapports et autres enregistrements passés et présents. Le programme a aussi généré de nombreux documents, rapports et mémoires. Enfin, des publications internationales et des thèses de doctorat sont en cours de réalisation ou abouties. Afin de rester lisible, nous avons donc choisi de présenter ce rapport 2014 sous la forme de 12 exemples parmi les travaux en cours. Ces exemples sont traités selon un schéma identique en 4 points simples : (i) La présentation du cas d’étude dans le projet global ; (ii),Les approches innovatrices développées ; (iii),La présentation de résultats marquants ; (iv),Les délivrables de STARECAPMED pour les politiques publiques. [less ▲]

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See detailProteome variations in Macrobrachium rosenbergii exposed to chlordecone: a gel-free proteomic approach
Lafontaine, Anne ULg; De Pauw, Edwin ULg; Forget-Leray, Joelle et al

Poster (2015, May 06)

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See detailThe effects of perinatal exposure to polychlorinated biphenyls on hippocampal neurogenesis
Pinson, Anneline ULg; Parent, Anne-Simone ULg; chatzi, christina et al

Poster (2015, May)

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