References of "Thiry, Marc"
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See detailBiologie cellulaire: exercices et méthodes
Thiry, Marc ULg; Rigo, Pierre ULg; Racano, Sandra

Book published by Dunod - 2ème édition (2016)

Cet ouvrage propose aux étudiants des premières années d’études supérieures une méthode progressive et conviviale pour comprendre et appliquer les concepts fondamentaux de la biologie cellulaire. À la ... [more ▼]

Cet ouvrage propose aux étudiants des premières années d’études supérieures une méthode progressive et conviviale pour comprendre et appliquer les concepts fondamentaux de la biologie cellulaire. À la suite de rappels de cours, sous forme de fiches, chaque chapitre propose des exercices de difficulté croissante pour s’évaluer : QCM, questions Vrai/Faux et exercices de synthèse. Les corrigés détaillés mettent en évidence la méthodologie. Des ressources numériqes avec des exercices d’entraînement supplémentaires complètent l’ouvrage. Cette nouvelle édition, actualisée, s'enrichit de nouveaux QCM, Vrai/Faux et exercices. [less ▲]

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See detailBiologie moléculaire: exercices et méthodes
Thiry, Marc ULg; Bourmeyster, Nicolas; Dommes, Jacques ULg et al

Book published by Dunod (2016)

Cet ouvrage constitue une méthode progressive et efficace pour comprendre et appliquer les concepts fondamentaux de la biologie moléculaire. Il propose : des rappels de cours sous forme de fiches, des ... [more ▼]

Cet ouvrage constitue une méthode progressive et efficace pour comprendre et appliquer les concepts fondamentaux de la biologie moléculaire. Il propose : des rappels de cours sous forme de fiches, des exercices pour s'évaluer (QCM, questions Vrai/Faux, exercices de synthèse...), des corrigés détaillés. Des exercices d’entraînement supplémentaires sont téléchargeables sur le site dunod.com. [less ▲]

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See detailRole of vimentin in the hearing organ
Renauld, Justine ULg; Francart, Marie-Emilie ULg; Thelen, Nicolas ULg et al

Conference (2016, January 25)

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See detailExpanding the clinical and mutational spectrum of the Ehlers-Danlos syndrome Dermatosparaxis.
Van Damme, Tim; Colige, Alain ULg; Syx, Delfien et al

in Genetics in Medicine : Official Journal of the American College of Medical Genetics (2016)

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See detailChanges in the structure and the composition of microtubules within supporting cells of the organ of hearing during development.
Renauld, Justine ULg; Freeman, Stephen ULg; Thelen, Nicolas ULg et al

Poster (2016)

The auditory organ is a highly specialized structure composed by specific cellular types. The sensory cells are characterized by stereocilia at their apex and are necessary for the sound perception. These ... [more ▼]

The auditory organ is a highly specialized structure composed by specific cellular types. The sensory cells are characterized by stereocilia at their apex and are necessary for the sound perception. These cells are supported by supporting cells which contain up to 3000 microtubules and are formed by 15protofilaments (15pf) instead of the canonical 13, a unique fact among vertebrates. It was shown that a β-tubulin isoform can specify the microtubule architecture, such as the expression of the Moth β2 in the Drosophila testes imposed the 16pf structure on the corresponding subset of Drosophila microtubules, which normally contain 13pf. To determine the role of these tubulins in the auditory organ and their possible involvement in the formation of the unusual 15pf microtubules of supporting cells, we studied the spatiotemporal localization of five -tubulin isotypes (β1 to 5) as well as acetyl-α-tubulin within the hearing organ during development in rodents. By using confocal microscopy, we showed that with the exception of the β3-tubulin isoform that was specific to nerve fibres, all the different β-tubulin isoforms and acetyl-α-tubulin were mainly present in the supporting cells. Contrary to β1-4-tubulins, we also found that the β5-tubulin isoform appeared only at a key stage of the postnatal development. By using transmission electron microscopy, we examined the fine structure of microtubules at an early and a late postnatal stage. Our TEM study indicated that these cells are composed by 13pf microtubules at P2, but by 15pf microtubules at P25. We revealed further that this developmental stage coincide with the formation of two separate bundles of microtubules from a unique one in these supporting cells. In conclusion, the architecture and composition of microtubules present in the supporting cells change during development of the organ of Corti. Further experiments are now required to determine if these changes are related to the appearance of β5-tubulin. [less ▲]

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See detailTissue factor induced by epithelial-mesenchymal transition triggers a pro-coagulant state that drives metastasis of circulating tumor cells.
Bourcy, Morgane ULg; Suarez-Carmona, Meggy ULg; Lambert, Justine ULg et al

in Cancer Research (2016)

Epithelial-mesenchymal transition (EMT) is prominent in circulating tumor cells (CTC), but how it influences metastatic spread in this setting is obscure. Insofar as blood provides a specific ... [more ▼]

Epithelial-mesenchymal transition (EMT) is prominent in circulating tumor cells (CTC), but how it influences metastatic spread in this setting is obscure. Insofar as blood provides a specific microenvironment for tumor cells, we explored a potential link between EMT and coagulation that may provide EMT-positive CTC with enhanced colonizing properties. Here we report that EMT induces tissue factor (TF), a major cell-associated initiator of coagulation and related pro-coagulant properties in the blood. TF blockade by antibody or shRNA diminished the pro-coagulant activity of EMT-positive cells, confirming a functional role for TF in these processes. Silencing the EMT transcription factor ZEB1 inhibited both EMT-associated TF expression and coagulant activity, further strengthening the link between EMT and coagulation. Accordingly, EMT-positive cells exhibited a higher persistance/survival in the lungs of mice colonized after intravenous injection, a feature diminished by TF or ZEB1 silencing. In tumor cells with limited metastatic capability, enforcing expression of the EMT transcription factor Snail increased TF, coagulant properties and early metastasis. Clinically, we identified a subpopulation of CTC expressing vimentin and TF in the blood of metastatic breast cancer patients consistent with our observations. Overall, our findings define a novel EMT-TF regulatory axis which triggers local activation of coagulation pathways to support metastatic colonization of EMT-positive CTC. [less ▲]

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See detailMelanoma antigen-D2: a nucleolar protein undergoing delocalization during cell cycle and after cellular stress
Pirlot, Céline ULg; Thiry, Marc ULg; Trussart, Charlotte ULg et al

in BBA Molecular Cell Research (2016), 1853(3), 581-595

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See detailStudy of intact virus-like particles of human papillomavirus by capillary electrophoresis
Bettonville, Virginie ULg; Nicol, Jérôme ULg; Thelen, Nicolas ULg et al

in Electrophoresis (2016), 37

Virus-like particles of human papillomavirus (HPV-VLP), resulting from the self-assembly of the capsid proteins (L1 or L1 and L2), have been widely used to study HPV as they are similar to the native ... [more ▼]

Virus-like particles of human papillomavirus (HPV-VLP), resulting from the self-assembly of the capsid proteins (L1 or L1 and L2), have been widely used to study HPV as they are similar to the native virion. Moreover, two prophylactic vaccines, Gardasil® and Cervarix®, are based on HPV-VLP L1. Analytical techniques currently used to characterize HPV-VLP, such as SDS-PAGE, Western blot, ELISA, are time-consuming and semi-quantitative. In this study, capillary electrophoresis (CE) was evaluated for the analysis of intact HPV16-VLP. The usefulness of capillary inner wall coating as well as various BGEs, pH and detergent additives were investigated. Reproducible HPV-VLP analysis in CE was achieved using poly(ethylene oxide) coated capillary and a BGE containing high salt concentration and low SDS concentration. The developed method enables HPV-VLP detection in less than 10 min (migration times RSD : 1.6 %). The identity of HPV-VLP peak was confirmed by comparison with a sample obtained from a wild-type baculovirus and with VLP-based vaccine, Gardasil®, after adjuvant dissolution. Finally, we applied the developed methodology to VLP-based vaccines, demonstrating that CE could be successfully used for vaccine quality control. [less ▲]

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See detailMyoferlin plays a key role in VEGFA secretion and impacts tumor-associated angiogenesis in human pancreas cancer
Fahmy, Karim ULg; Gonzalez, Arnaud; Arafa, Mohammad et al

in International Journal of Cancer = Journal International du Cancer (2016), 138

Pancreatic ductal adenocarcinoma is one of the most deadly forms of cancers with no satisfactory treatment to date. Recent studies have identified myoferlin, a ferlin family member, in human pancreas ... [more ▼]

Pancreatic ductal adenocarcinoma is one of the most deadly forms of cancers with no satisfactory treatment to date. Recent studies have identified myoferlin, a ferlin family member, in human pancreas adenocarcinoma where its expression was associated to a bad prognosis. However, the function of myoferlin in pancreas adenocarcinoma has not been reported. In other cell types, myoferlin is involved in several key plasma membrane processes such as fusion, repair, endocytosis and tyrosine kinase receptor activity. In this study, we showed that myoferlin silencing in BxPC-3 human pancreatic cancer cells resulted in the inhibition of cell proliferation in vitro and in a significant reduction of the tumor volume in chick chorioallantoic membrane assay. In addition to be smaller, the tumors formed by the myoferlin-silenced cells showed a marked absence of functional blood vessels. We further demonstrated that this effect was due, at least in part, to an inhibition of VEGFA secretion by BxPC-3 myoferlin-silenced cells. Using immunofluorescence and electron microscopy, we linked the decreased VEGFA secretion to an impairment of VEGFA exocytosis. The clinical relevance of our results was further strengthened by a significant correlation between myoferlin expression in a series of human pancreatic malignant lesions and their angiogenic status evaluated by the determination of the blood vessel density. [less ▲]

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See detailMelanoma antigen D2, a substrate of ATM, is a nucleolar protein that shuttles with cell cycle and cellular stress.
Pirlot, Céline; Thiry, Marc ULg; Trussart, Charlotte ULg et al

Poster (2015, November 03)

ATM coordinates numerous facets of the highly regulated DDR network. In order to identify new ATM substrates we have performed a yeast two hybrid experiment with HEAT domains of ATM and an HeLa cDNA ... [more ▼]

ATM coordinates numerous facets of the highly regulated DDR network. In order to identify new ATM substrates we have performed a yeast two hybrid experiment with HEAT domains of ATM and an HeLa cDNA library. Melanoma antigen D2 (MAGED2) was one of the interacting proteins identified in this screen. MAGED2 belongs to the type II Melanoma AntiGEn (MAGE) family. MAGE homology domain, shared by all MAGE proteins, was shown to enhance E3 ligase activity. Actually, little is known about MAGED2 functions. Like every type II MAGE protein, it is expressed in all adult tissues. However, MAGE-D2 has the particularity to be over-expressed in numerous primary cancer and metastasis and it is now recognized as a tumour marker. This over-expression suggests that MAGED2 could be important for the process of cancerisation. MAGED2 was also shown to be a negative regulator of p53, but we did not confirm this property. Proteomic analyses also detected numerous phosphorylated or acetylated residues in response to stress and during cell cycle progression, suggesting a role in cellular signal transduction. We identified the residues targeted by ATM in MAGE-D2 and analysed the localisation of MAGED2 during the interphase and after genotoxic/nucleolar stresses. [less ▲]

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See detailHypervulnerability to Sound Exposure through Impaired Adaptive Proliferation of Peroxisomes.
Delmaghani, Sedigheh; Defourny, Jean; Aghaie, Asadollah et al

in Cell (2015), 163(4), 894-906

A deficiency in pejvakin, a protein of unknown function, causes a strikingly heterogeneous form of human deafness. Pejvakin-deficient (Pjvk(-/-)) mice also exhibit variable auditory phenotypes ... [more ▼]

A deficiency in pejvakin, a protein of unknown function, causes a strikingly heterogeneous form of human deafness. Pejvakin-deficient (Pjvk(-/-)) mice also exhibit variable auditory phenotypes. Correlation between their hearing thresholds and the number of pups per cage suggest a possible harmful effect of pup vocalizations. Direct sound or electrical stimulation show that the cochlear sensory hair cells and auditory pathway neurons of Pjvk(-/-) mice and patients are exceptionally vulnerable to sound. Subcellular analysis revealed that pejvakin is associated with peroxisomes and required for their oxidative-stress-induced proliferation. Pjvk(-/-) cochleas display features of marked oxidative stress and impaired antioxidant defenses, and peroxisomes in Pjvk(-/-) hair cells show structural abnormalities after the onset of hearing. Noise exposure rapidly upregulates Pjvk cochlear transcription in wild-type mice and triggers peroxisome proliferation in hair cells and primary auditory neurons. Our results reveal that the antioxidant activity of peroxisomes protects the auditory system against noise-induced damage. [less ▲]

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See detailOutils d’aide à la réussite dans le cadre du cours de Biologie en Premier bachelier en Faculté des Sciences à l’Université de Liège : présentation et analyse
Thiry, Marc ULg; Rigo, Pierre ULg; Poffé, Corentin ULg

Conference (2015, August 26)

A l’Université de Liège, les cours du premier quadrimestre sont communs à certaines sections du premier bachelier de la Faculté des Sciences. Le programme de ce quadrimestre commun reprend un cours de ... [more ▼]

A l’Université de Liège, les cours du premier quadrimestre sont communs à certaines sections du premier bachelier de la Faculté des Sciences. Le programme de ce quadrimestre commun reprend un cours de biologie. Depuis plusieurs années, le titulaire de ce cours de biologie met en place diverses actions visant à favoriser la transition secondaire-université : séminaires, remédiations, aides à l’étude, forum, livre de référence, etc. Ces différentes actions font l’objet d’un suivi didactique, permettant un monitoring précis et une régulation au fil du temps. Dans un premier temps, la communication présentera l’état des lieux des outils mis à la disposition des étudiants en vue de favoriser leur réussite pour le cours de biologie, mais aussi pour les autres cours, par transfert des compétences acquises. Dans un second temps, le dispositif de suivi didactique des séances d'aide à l'étude et ses principaux résultats seront présentés. [less ▲]

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See detailSpatio‑temporal dynamics of β‑tubulin isotypes during the development of the sensory auditory organ in rat
Renauld, Justine ULg; Johnen, Nicolas; Thelen, Nicolas ULg et al

in Histochemistry & Cell Biology (2015)

There are different β-tubulin isoforms in microtubules of vertebrate tissues. However, their functional significance is still largely unknown. In the present study, we investigated the localization of ... [more ▼]

There are different β-tubulin isoforms in microtubules of vertebrate tissues. However, their functional significance is still largely unknown. In the present study, we investigated the localization of five β-tubulin isotypes (β1– 5) within the hearing organ during development in rat. By using confocal microscopy, we showed that with the exception of the β3-tubulin isoform that was specific to nerve fibres, all the different β-tubulin isoforms were mainly present in the supporting cells. Contrary to β1–4-tubulins, we also found that the β5-tubulin isoform appeared only at a key stage of the post-natal development in specific cell types (pillar cells and Deiters’ cells). By using transmission electron microscopy, we revealed further that this developmental stage coincided with the formation of two separate bundles of microtubules from a unique one in these supporting cells. Together, these results suggest that the β5-tubulin isoform might be involved in the generation of new microtubule bundles from a pre-existing one. [less ▲]

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See detailIdentification of VZV ORF9p potential cellular partners that could be important for the viral egress.
Lebrun, Marielle ULg; riva, laura; Rambout, Xavier ULg et al

Poster (2015, July 26)

ORF9p (homologous to HSV-1 VP22) is a VZV tegument protein essential for the viral replication. During the lytic cycle it is the mostly expressed gene. We have recently demonstrated that it is a substrate ... [more ▼]

ORF9p (homologous to HSV-1 VP22) is a VZV tegument protein essential for the viral replication. During the lytic cycle it is the mostly expressed gene. We have recently demonstrated that it is a substrate of the viral kinase ORF47p and that its ORF47p-dependent phosphorylation is important for the secondary envelopment process. We also have identified an acidic cluster (AC) within the protein that is important for its correct localization in the infected cells and for the interaction with ORF47p. The recombinant VZV expressing ORF9p-ΔAC presents an accumulation of capsids in the perinuclear space. ORF9p seems then to play an important role in several steps of the egress process. In this context, we sought to identify cellular partners of ORF9p that might be important for these functions. We performed a yeast two hybrid screen against the human ORFeome 5.1. and picked out 44 candidates among which 5 proteins playing roles in membrane organization and targeting. We currently are trying to confirm these interactions in infected cells and to assess the role of these interactions for the viral lytic cycle. [less ▲]

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