References of "Tarayre, Cédric"
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See detailASTINPROD project – Production of an anticancer molecule by the symbiotic fungus Villosirosea asteris
Vassaux, Antoine; Tarayre, Cédric ULg

Scientific conference (2017, June)

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See detailAn anticancer molecule stemming from a plant root produced in a bioreactor
Vassaux, Antoine; Tarayre, Cédric ULg; Delvigne, Frank ULg et al

Poster (2017, May 24)

Secondary metabolites produced by plants or their symbionts have already shown specific properties: anticancer, anti-inflammatory, antibacterial, antifungal effects, etc. Astin C is a non-ribosomal ... [more ▼]

Secondary metabolites produced by plants or their symbionts have already shown specific properties: anticancer, anti-inflammatory, antibacterial, antifungal effects, etc. Astin C is a non-ribosomal peptide (secondary metabolite) produced by the fungus Villosirosea asteris, endosymbiont of the medicinal plant Aster tataricus, which has shown an interesting anticancer activity. The current challenge is the production of the molecule on a large scale and in higher quantities, either from the original fungus through fermentation technologies, or by a heterologous yeast strain having integrated the genes involved in the astin C biosynthesis pathway. The purpose of this workshop is to highlight the implementation possibilities of a strain producing a metabolite of interest. [less ▲]

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See detailLooking for phosphate-accumulating bacteria in activated sludge processes : a multidisciplinary approach
Tarayre, Cédric ULg; Charlier, Raphaëlle; Delepierre, Anissa ULg et al

in Environmental Science and Pollution Research (2017)

Over the past decades, an increasing need in renewable resources has progressively appeared. This trend concerns not only fossil fuels but also mineral resources. Wastewater and sewage sludge contain ... [more ▼]

Over the past decades, an increasing need in renewable resources has progressively appeared. This trend concerns not only fossil fuels but also mineral resources. Wastewater and sewage sludge contain significant concentrations in phosphate and can be considered as a fertilizer source of the utmost importance. In wastewater treatment plants, the biological uptake of phosphate is performed by a specific microbiota: the phosphate-accumulating organisms. These microorganisms are recovered in sewage sludge. Here, we aimed to investigate the occurrence of phosphate accumulators in four wastewater treatment plants. A 16S metagenetic analysis identified the main bacterial phyla extracted from the aerobic treatment: α-Proteobacteria, β-Proteobacteria, and Sphingobacteria. An enrichment stage was performed to stimulate the specific growth of phosphate-accumulating bacteria in an acetate medium. An analysis of metabolic activities of sulfur and phosphorus highlighted strong modifications related to phosphorus and much less distinguishable effects with sulfur. A solid acetate medium containing 5-Br-4-Cl-3-indolyl phosphate was used to select potential phosphate-accumulating bacteria from the enriched consortia. The positive strains have been found to belong in the genera Acinetobacter, Corynebacterium, and Pseudomonas. Finally, electron microscopy was applied to the strains and allowed to confirm the presence of polyphosphate granules. Some of these bacteria contained granules the size of which exceeded 100 nm. [less ▲]

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See detailThe Effect of Nutrients on the Degradation of Hydrocarbons in Mangrove Ecosystems by Microorganisms
Semboung Lang, Firmin ULg; Tarayre, Cédric ULg; Destain, Jacqueline ULg et al

in International Journal of Environmental Research (2016), 10(4), 583-592

Mangrove ecosystems are areas prone to various types of pollution, especially hydrocarbons. These hydrocarbons mostly stem from human activities such as spills coming from offshore oil operations, runoff ... [more ▼]

Mangrove ecosystems are areas prone to various types of pollution, especially hydrocarbons. These hydrocarbons mostly stem from human activities such as spills coming from offshore oil operations, runoff from surrounding urban areas or atmospheric deposition. This pollution causes the decline of mangroves, which results in an imbalance in the functioning of this particular ecosystem with damages to the microbiota. Biodegradation allows to restore these ecosystems. This biodegradation can only be effective in specific environmental conditions. The presence of nutrients, which stimulate bacterial growth and promote biodegradation, is a key parameter to be considered. During this experiment, we achieved biodegradation tests to assess the effect of nitrogen and phosphorus on the process. The results showed that the biodegradation rates were strongly bound to the presence of nutrients. The degradation rates depended on the medium. The treatment that reached the best rate of degradation of diesel after 10 days was the one using 20% of a nutrient solution (MSM) containing nitrogen and phosphorus. This treatment led to a maximal degradation of 84.7% ± 4.7% obtained in the flasks containing 20% of a nutrient solution (MSM) containing nitrogen and phosphorus. [less ▲]

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See detailCharacterisation of Phosphate Accumulating Organisms and Techniques for Polyphosphate Detection: A Review
Tarayre, Cédric ULg; Nguyen, Huu-Thanh; Brognaux, Alison ULg et al

in Sensors (2016), 16(797), 1-14

Phosphate minerals have long been used for the production of phosphorus-based chemicals used in many economic sectors. However, these resources are not renewable and the natural phosphate stocks are ... [more ▼]

Phosphate minerals have long been used for the production of phosphorus-based chemicals used in many economic sectors. However, these resources are not renewable and the natural phosphate stocks are decreasing. In this context, the research of new phosphate sources has become necessary. Many types of wastes contain non-negligible phosphate concentrations, such as wastewater. In wastewater treatment plants, phosphorus is eliminated by physicochemical and/or biological techniques. In this latter case, a specific microbiota, phosphate accumulating organisms (PAOs), accumulates phosphate as polyphosphate. This molecule can be considered as an alternative phosphate source, and is directly extracted from wastewater generated by human activities. This review focuses on the techniques which can be applied to enrich and try to isolate these PAOs, and to detect the presence of polyphosphate in microbial cells. [less ▲]

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See detailStudy of microbial phenotypic heterogeneity under bioprocess conditions using « single-cell » techniques
Delepierre, Anissa ULg; Brognaux, Alison ULg; Baert, Jonathan ULg et al

Poster (2016, May 20)

Presentation of single-cell techniques in order to characterize microbial phenotypic heterogeneity: fluorescent labelling, biosensors, automated flow cytometry combined to proteomics approach.

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See detailNew perspectives for the design of sustainable bioprocesses for phosphorus recovery from waste
Tarayre, Cédric ULg; De Clercq, Lies; Charlier, Raphaëlle et al

in Bioresource Technology (2016), 206

Phosphate rock has long been used for the production of phosphorus based chemicals. However, considering the depletion of the reservoirs and the decrease of the quality of phosphate rocks, a potential ... [more ▼]

Phosphate rock has long been used for the production of phosphorus based chemicals. However, considering the depletion of the reservoirs and the decrease of the quality of phosphate rocks, a potential market is now emerging for the recovery of phosphate from waste and its reuse for different applications. Notably, phosphate recovery from wastewater could be included in a circular economy approach. This review focuses on the use of microbial systems for phosphorus accumulation and recovery, by considering the actual range of analytical techniques available for the monitoring of phosphorus accumulating organisms, as well as the actual biochemical and metabolic engineering toolbox available for the optimization of bioprocesses. In this context, knowledge gathered from process, system and synthetic biology could potentially lead to innovative process design. [less ▲]

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See detailTechniques for nutrient recovery from manure and slurry
Camargo-Valero, Miller; De Clercq, Lies; Delvigne, Frank ULg et al

Report (2015)

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See detailTechniques for nutrient recovery from digestate derivatives
Bamelis, Lies; Blancke, Stieven; Camargo-Valero, Miller et al

Report (2015)

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See detailTechniques for nutrient recovery from household and industrial wastes
Camargo-Valero, Miller; Bamelis, Lies; De Clercq, Lies et al

Report (2015)

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See detailFluorescent reporter libraries as useful tools for optimizing microbial cell factories: a review of the current methods and applications
Delvigne, Frank ULg; Pêcheux, Hélène; Tarayre, Cédric ULg

in Frontiers in Bioengineering and Biotechnology (2015), 3

The use of genetically encoded fluorescent reporters allows speeding up the initial optimization steps of microbial bioprocesses. These reporters can be used for determining the expression level of a ... [more ▼]

The use of genetically encoded fluorescent reporters allows speeding up the initial optimization steps of microbial bioprocesses. These reporters can be used for determining the expression level of a particular promoter, not only the synthesis of a specific protein but also the content of intracellular metabolites. The level of protein/metabolite is thus proportional to a fluorescence signal. By this way, mean expression profiles of protein/ metabolites can be determined non-invasively at a high-throughput rate, allowing the rapid identification of the best producers. Actually, different kinds of reporter systems are available, as well as specific cultivation devices allowing the on-line recording of the fluorescent signal. Cell-to-cell variability is another important phenomenon that can be integrated into the screening procedures for the selection of more efficient microbial cell factories. [less ▲]

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See detailBIOREFINE Project – Recovery of useful nutrients from organic wastes produced in five European countries (3AV.3.55)
Tarayre, Cédric ULg; De Clercq, Lies; Michels, Evi et al

Poster (2015, June 01)

At this time, many wastes are used or eliminated through processes that do not really consider their potential applications. Such wastes contain useful nutrients (nitrogen, phosphorus and potassium), the ... [more ▼]

At this time, many wastes are used or eliminated through processes that do not really consider their potential applications. Such wastes contain useful nutrients (nitrogen, phosphorus and potassium), the importance of which has been demonstrated in agriculture for many years. The composition of wastes is highly heterogeneous, which makes treatment techniques more difficult to apply on a large scale. Sewage sludge is usually used as a fertilizer in agriculture, in energy production or in the field of construction. The main use of manure is agriculture, although considerable amounts of nutrients are lost and cause pollution. Digestate is also used in agriculture, but other alternatives have been proposed. Ashes should also be highlighted, although they do not contain nitrogen, which is lost into the atmosphere during the combustion process. Finally, household and industrial wastes are resources that should be considered as well. Those different types of wastes could be recycled to produce environment-friendly fertilizers. Here, we propose to investigate these opportunities inside five European countries (Belgium, France, Germany, United Kingdom and The Netherlands) through five work packages with the BioRefine Project. [less ▲]

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See detailMultiple analyses of microbial communities applied to the gut of the wood-feeding termite Reticulitermes flavipes fed on artificial diets
Tarayre, Cédric ULg; Bauwens, Julien ULg; Mattéotti, Christel et al

in Symbiosis (2015)

The purpose of this work was the observation of the differences between the microbial communities living in the gut of the termite Reticulitermes flavipes fed on different diets. The termites were fed on ... [more ▼]

The purpose of this work was the observation of the differences between the microbial communities living in the gut of the termite Reticulitermes flavipes fed on different diets. The termites were fed on poplar wood (original diet) and artificial diets consisting of crystalline cellulose (with and without lignin), α-cellulose (with and without lignin) and xylan. The termites were then dissected and the protist communities were analyzed through microscopy, leading to the conclusion that protist species are strongly influenced by diets. BIOLOG ECO Microplates® were used to assess the metabolic properties of the different types of consortia, highlighting strong differences on the basis of principal component analysis and calculation of similarity rates. The microorganisms were cultivated in liquid media corresponding to the artificial diets before being characterized through a metagenetic analysis of gut microbiota (16S ribosomal DNA). This analysis identified several phyla: Acidobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Fibrobacteres, Firmicutes, Nitrospirae, OP9, Planctomycetes, Proteobacteria, Spirochaetes, TM6, Tenericutes, Verrucomicrobia and WS3. The OTUs were also determined and confirmed the abundance of Proteobacteria, Bacteroidetes, Firmicutes and Verrucomicrobia. It was possible to isolate several strains from the liquid media, and one bacterium and several fungi were found to produce interesting enzymatic activities. The bacterium Chryseobacterium sp. XAvLW produced α-amylase, β-glucosidase, endo-1,4-β-D-glucanase, endo-1,4-β-D-xylanase and filter paper-cellulase, while the fungi Sarocladium kiliense CTGxxyl and Trichoderma virens CTGxAviL generated the same activities added with endo-1,3-β-D-glucanase. [less ▲]

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See detailDetermination of Zinc, Cadmium and Lead Bioavailability in Contaminated Soils at the Single-Cell Level by a Combination of Whole-Cell Biosensors and Flow Cytometry
Hurdebise, Quentin ULg; Tarayre, Cédric ULg; Fischer, Christophe ULg et al

in Sensors (2015)

Zinc, lead and cadmium are metallic trace elements (MTEs) that are widespread in the environment and tend to accumulate in soils because of their low mobility and non-degradability. The purpose of this ... [more ▼]

Zinc, lead and cadmium are metallic trace elements (MTEs) that are widespread in the environment and tend to accumulate in soils because of their low mobility and non-degradability. The purpose of this work is to evaluate the applicability of biosensors as tools able to provide data about the bioavailability of such MTEs in contaminated soils. Here, we tested the genetically-engineered strain Escherichia coli pPZntAgfp as a biosensor applicable to the detection of zinc, lead and cadmium by the biosynthesis of green fluorescent protein (GFP) accumulating inside the cells. Flow cytometry was used to investigate the fluorescence induced by the MTEs. A curvilinear response to zinc between 0 and 25 mg/L and another curvilinear response to cadmium between 0 and 1.5 mg/L were highlighted in liquid media, while lead did not produce exploitable results. The response relating to a Zn2+/Cd2+ ratio of 10 was further investigated. In these conditions, E. coli pPZntAgfp responded to cadmium only. Several contaminated soils with a Zn2+/Cd2+ ratio of 10 were analyzed with the biosensor, and the metallic concentrations were also measured by atomic absorption spectroscopy. Our results showed that E. coli pPZntAgfp could be used as a monitoring tool for contaminated soils being processed. [less ▲]

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See detailWood digestion in lower termites: multidisciplinary approaches based on differential feeding
Bauwens, Julien ULg; Brasseur, Catherine ULg; Tarayre, Cédric ULg et al

Poster (2014, December)

Termites digestive tract and hindgut especially still holds many secrets despites hundreds of years of research. The complexity of the symbiotic microbial community and the contrast of physio-chemical ... [more ▼]

Termites digestive tract and hindgut especially still holds many secrets despites hundreds of years of research. The complexity of the symbiotic microbial community and the contrast of physio-chemical environments found in lower termites paunch are potentially the key point to explain the efficiency of ligno-cellulose digestion. Contribution of advancing technologies accelerates the progress of our knowledge in this field. Here, we present multiple approaches combining old and recent techniques used to highlight the effect of ligno-cellulosic compounds on termite gut and the role of populations from the symbiotic microbial community. Termites Reticulitermes flavipes (Kollar) submitted to various artificial diets showed variations in flagellates populations profile and enzymatic activities. Differential protein expression was investigated using 2D-DIGE MALDI-TOF-TOF and 2D-LC-MS/MS using high resolution orbitrap analyzer. Results from both proteomic experiments tend to support each-other and bring complementary points of view. The gel-free analysis resulted in highly contrasted identification of enzymes involved in ligno-cellulose digestion and metabolism. Finally, differential feeding experiments leaded to in vivo selection of different symbiotic communities. These communities were characterized following some metabolism assays and allowed the cultivation of diverse microbial consortia using media closely related to the respective artificial diets. This work provides relevant data on termite and associated microbial community response to alimentary diets. [less ▲]

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See detailÉTUDE DU COMPLEXE ENZYMATIQUE DES SYMBIONTES DU TERMITE RETICULITERMES FLAVIPES (EX. SANTONENSIS)
Tarayre, Cédric ULg

Doctoral thesis (2014)

In the light of the economic, environmental and social context in which we live today, it has become obvious that the use of fossil fuels is not conceivable over the long term. Some alternatives have ... [more ▼]

In the light of the economic, environmental and social context in which we live today, it has become obvious that the use of fossil fuels is not conceivable over the long term. Some alternatives have therefore emerged in recent years. Second-generation biofuels are one of those alternatives and are based on the exploitation of vegetal biomass, also called lignocellulosic biomass. These materials require a hydrolysis step which can notably be achieved by enzymes. Some insects, such as termites, harbor complex microbial communities inside their digestive tracts. Those communities are able to produce enzymes which can be used in the field of the hydrolysis of vegetal biomass. This is what this thesis deals with. The main part of the work done focused on the research of enzyme-producing microorganisms (bacteria, mycetes and protists) responsible for the degradation of hemicelluloses and cellulose, the hydrolysis of which releases fermentable sugars. The insect which was used in this work was Reticulitermes flavipes (ex. santonensis), a lower termite, harboring a highly diversified internal microflora. This thesis describes the characterization of the microbial strains which were isolated and the enzymes they secrete. A complementary part of this research focussed on termites grown on artificial diets. The objective of this part was multiple : isolating enzyme-producing strains, not extractable according to the standard technique, and characterizing the microflora resulting from the applied artificial diets. This multidisciplinary approach was based on microscopy, proteomics, metagenomics and the assessment of metabolism applied to the different microbial consortia. [less ▲]

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